The fabrication of functional microcarriers capable of achieving in vivo-like three-dimensional cell culture is important for many tissue engineering applications. Here,inspired by the structure of Buddha beads, which...The fabrication of functional microcarriers capable of achieving in vivo-like three-dimensional cell culture is important for many tissue engineering applications. Here,inspired by the structure of Buddha beads, which are generally composed of moveable beads strung on a rope, we present novel cell microcarriers with controllable macropores and heterogeneous microstructures by using a capillary array microfluidic technology. Microfibers with a string of moveable and releasable microcarriers could be achieved by an immediate gelation reaction of sodium alginate spinning and subsequent polymerization of cell-dispersed gelatin methacrylate emulsification. The sizes of the microcarriers and their inner macropores could be well tailored by adjusting the flow rates of the microfluidic phases; this was of great importance in guaranteeing a sufficient supply of nutrients during cell culture. In addition, by infusing multiple cell-dispersed pregel solutions into the capillaries, the microcarriers with spatially heterogeneous cell encapsulations for mimicking physiological structures and functions could also be achieved.展开更多
Porous Fe3O4 sub-micro particles with sphere-like, cube-like and walnut-like morphologies were obtained by a two-step process, and the electromagnetic properties of the Fe3O4 particle/wax composites were investigated....Porous Fe3O4 sub-micro particles with sphere-like, cube-like and walnut-like morphologies were obtained by a two-step process, and the electromagnetic properties of the Fe3O4 particle/wax composites were investigated. The reflect loss was less than -20 dB for all of the composites in different frequency ranges. The cube-like and walnut-like Fe3O4 composites exhibit improved complex permittivity and permeability and dual-frequency and wide bandwidth absorption characteristics, which is mainly attributed to the larger shape anisotropy. Such a high absorption property indicates that these porous Fe3O4 particles with various morphologies are very promising for electromagnetic wave absorptive materials.展开更多
基金supported by the National Natural Science Foundation of China(21473029 and 51522302)the NSAF Foundation of China(U1530260)+3 种基金the Natural Science Foundation of Jiangsu(BK20140028)the Program for New Century Excellent Talents in Universitythe Scientific Research Foundation of Southeast Universitythe Scientific Research Foundation of Graduate School of Southeast University
文摘The fabrication of functional microcarriers capable of achieving in vivo-like three-dimensional cell culture is important for many tissue engineering applications. Here,inspired by the structure of Buddha beads, which are generally composed of moveable beads strung on a rope, we present novel cell microcarriers with controllable macropores and heterogeneous microstructures by using a capillary array microfluidic technology. Microfibers with a string of moveable and releasable microcarriers could be achieved by an immediate gelation reaction of sodium alginate spinning and subsequent polymerization of cell-dispersed gelatin methacrylate emulsification. The sizes of the microcarriers and their inner macropores could be well tailored by adjusting the flow rates of the microfluidic phases; this was of great importance in guaranteeing a sufficient supply of nutrients during cell culture. In addition, by infusing multiple cell-dispersed pregel solutions into the capillaries, the microcarriers with spatially heterogeneous cell encapsulations for mimicking physiological structures and functions could also be achieved.
基金supported by the National Natural Science Foundation of China (Grant Nos. 51072038, 50772025 and 21001035)NECT, Outstanding Youth Foundation of Heilongjiang Province (Grant No. JC201008)+4 种基金the Natural Science Foundation of Heilongjiang Province, China (Grant No. F200828)the Ministry of Science and Technology of China (Grant No. 2008DFR20420)the Fundamental Research Funds for the Central Universities (Grant Nos. HEUCFT1010, HEUCF101016, HEUCF20111124 and HEUCF101016)the National Basic Research Program of China (Grant No. 2007CB310500)Harbin Key Sci-Tech Project (Grant No. 2010AA4BG004)
文摘Porous Fe3O4 sub-micro particles with sphere-like, cube-like and walnut-like morphologies were obtained by a two-step process, and the electromagnetic properties of the Fe3O4 particle/wax composites were investigated. The reflect loss was less than -20 dB for all of the composites in different frequency ranges. The cube-like and walnut-like Fe3O4 composites exhibit improved complex permittivity and permeability and dual-frequency and wide bandwidth absorption characteristics, which is mainly attributed to the larger shape anisotropy. Such a high absorption property indicates that these porous Fe3O4 particles with various morphologies are very promising for electromagnetic wave absorptive materials.
