The reorientation of microtubules (MTS) in roots of Oryza sativa L. treated with 2,4_D was studied using confocal laser scanning microscopy. In the control (the roots were not treated with 2,4_D), different distributi...The reorientation of microtubules (MTS) in roots of Oryza sativa L. treated with 2,4_D was studied using confocal laser scanning microscopy. In the control (the roots were not treated with 2,4_D), different distribution patterns of MTS were observed in the different growth zones of root tips. MTS of the cortical cells were randomly aligned in the zone of cell division. They were transversely arranged in the cortical cells of the zone of cell elongation; and obliquely oriented in the root hair zone. After treatment with 2,4_D, MTS displayed distinct changes with reorientation in the cortex of the root tip coupling with the inhibition of root growth. MTS changed their orientation in the cortical cells of the zone of cell division from being randomly oriented to transversely oriented when incubated in 1 mg/L 2,4_D for 1 h. However, they were recovered and became randomly oriented when the roots were treated with 1 mg/L 2,4_D for 24 h. An array of MTS, which was different from that in the control, was observed in the cortical cells of the zone of cell elongation in the roots treated with 1 mg/L 2,4_D for 1 h. After treatment with 10 mg/L 2,4_D for 1 h, the MTS in the cortical cells of the zone of cell division became transversely reorientated, but the pattern of MTS distribution was different from that in the 1_hour treatment of 1 mg/L 2,4_D. MTS were hardly detected in the same type of cells when roots were submerged in 10 mg/L 2,4_D for 24 h. When roots were incubated in 10 mg/L 2,4_D for 1 h, the MTS of the cortical cells in the zone of cell elongation became randomly oriented, and much more randomly when the roots were treated for 24 h.展开更多
We conducted experiments on specially designed microfluidic chips that generate droplets through a microfluidic ow-focusing approach. The fluid flow in the microfluidic channel produced a shear flow field at low Reyno...We conducted experiments on specially designed microfluidic chips that generate droplets through a microfluidic ow-focusing approach. The fluid flow in the microfluidic channel produced a shear flow field at low Reynolds numbers. The droplets in the microfluidic system exhibited special droplet pattern formations similar to periodic crystal-like lattices because of the competition between shear forces and surface tension. By adjusting the flow rate ratio of the water (droplet phase) to oil (continuous phase) phases and changing the outlet channel widths, the droplets formed monolayer dispersion to double-layer formation to monolayer squeezing when the outlet channel widths were 250 or 300 μm. We also obtained droplets with monolayer dispersion, three-layer arrangements, double-layer squeezing, and monolayer squeezing when the outlet channel width was 350 μm. The outlet channel width was increased to 400 μm, and four-layer arrangements were observed. We also studied the translation of droplet formation, which resulted in a detailed strategy to control drop size and droplet pattern formation for emulsi cation in microfluidic devices. We expect that our strategy can provide theoretical guidance to synthesize dispersion or polydisperse colloid particles.展开更多
To improve the sensitivity of protein microarray, a prism surface replaces the surface of the common microscope slide.The protein targets arrayed on the surface are hybridized and labelled by fluorescent probes. Evane...To improve the sensitivity of protein microarray, a prism surface replaces the surface of the common microscope slide.The protein targets arrayed on the surface are hybridized and labelled by fluorescent probes. Evanescent excitation occurs when the convergent laser reaches the surface, and a photomultiplier tube detects the emitted fluorescent signal. A two-dimensional actuator scans the whole surface to achieve planar laser excitation and fluorescence collection. The penetration depth of the evanescent field into the protein targets is only some hundred nanometers and can be controlled by different incident angle of the laser beam, so the undesired background signals are reduced dramatically and the detection sensitivity is improved by a factor of 50 to 100 comparing to confocal excitation. This approach can detect low abundance analytes without signal amplification.展开更多
Poly(vinylidene fluoride)(PVDF) is a semi-crystalline thermoplastic polymer with excellent thermal stability,electrochemical stability and corrosion resistance, which has been widely studied and applied in industrial ...Poly(vinylidene fluoride)(PVDF) is a semi-crystalline thermoplastic polymer with excellent thermal stability,electrochemical stability and corrosion resistance, which has been widely studied and applied in industrial nonmetallic heat exchanger and piezoelectric-film sensor. In this study, polyaniline(PANI) nanofibers were synthesized using dodecylbenzene sulfonic acid as the surfactant. The obtained PANI nanofibers were blended in PVDF matrix to enhance thermal conductivity and tensile strength of composite materials. Electric field was applied for the orientation of membrane structure during membrane formation. Scanning electron microscope(SEM) images exhibited that the PANI nanofibers were well-dispersed in the composite membranes. The structure of composite membranes was more orderly after alignment. X-ray diffraction(XRD) and differential scanning calorimetry(DSC) indicated that the content of PANI nanofibers contributed to the transformation of PVDF from α-phase to β-phase. Both the tensile strength and thermal conductivity of composite membranes were significantly improved. This tendency was further enhanced by the application of electric field. The maximum tensile strength was obtained when the content of PANI nanofibers was 3 wt%, which was 46.44% higher than that of pure PVDF membrane. The maximum thermal conductivity of composite membranes after alignment was 84.5% greater than that of pure PVDF membrane when the content of PANI nanofibers was 50 wt%. The composite membrane is a promising new potential material in heat transfer field and the mechanism explored in this study would be informative for further development of similar thermal conductive polymeric materials.展开更多
Substitution permutation network (SPN) is one important structure of block cipher cryptosystems. Prior work has shown different fault analyses on SPN. The formalization of fault analysis of both attack and protect on ...Substitution permutation network (SPN) is one important structure of block cipher cryptosystems. Prior work has shown different fault analyses on SPN. The formalization of fault analysis of both attack and protect on SPN have been given. The overhead and time tolerance of fault detection have been discussed. The pseudo-blinding method to detect fault attack is introduced, and the balance of the security, overhead and time tolerance based on the evaluation could be made.展开更多
The landslide disaster caused by the argillaceous interlayer not only destroys buildings,cultivated land,and roads but also seriously endangers human life and safety.This study concerns the mineral composition of sele...The landslide disaster caused by the argillaceous interlayer not only destroys buildings,cultivated land,and roads but also seriously endangers human life and safety.This study concerns the mineral composition of selected argillaceous interlayer and their strength characteristics.To study the mineral composition of argillaceous interlayers,8 kinds of samples in the southern Jiangsu region of China were analyzed utilizing X-ray diffraction(XRD).The repeated direct shear strength tests(RDST)were carried out on the undisturbed specimens of the argillaceous interlayer.The results show that the argillaceous interlayer with high content of kaolinite shows ductile failure mode,which means that there is no obvious residual strength in the shear process.The arrangement of mineral particles on the shear surface of the specimens after different shear displacements was observed under the scanning electron microscope(SEM).It was observed that mineral particles on the shear surface showed a more directional arrangement with the increase of shear displacement.Furthermore,the influence of shear direction on the argillaceous interlayer with completely oriented mineral particles was studied through numerical experiments with four shear strength mechanisms proposition proposed.The influence of the mineral arrangement on the action occasion and magnitude of dilatancy component of shear strength is clarified in the shear mechanism.展开更多
The combustion characteristics and particulate emission during combustion of Thai lignite with 30% of secondary air to total air (SA:TA) in a fixed bed combustor have been investigated in real-time. The results hav...The combustion characteristics and particulate emission during combustion of Thai lignite with 30% of secondary air to total air (SA:TA) in a fixed bed combustor have been investigated in real-time. The results have shown that particle formation is governed by competing reaction between the formation of the nucleated sized-particles (Dp 〈 0.1μm) and the coagulated particle (Dp 0.1-1 μm). Temperature and burning rate are the highest priority factors to control the emission of particulate. Furthermore, the co-firing of coal/rice husks at 60:40% mass fraction with 10%SA:TA could be the alternative options to further reduction of particulate and to be recommended.展开更多
Retina is a multilayer and highly specialized tissue important in converting light into neural signals. In humans, the critical period for the formation of complex multiplayer structure takes place during embryogenesi...Retina is a multilayer and highly specialized tissue important in converting light into neural signals. In humans, the critical period for the formation of complex multiplayer structure takes place during embryogenesis be- tween 12 and 28 weeks. The morphologic changes during retinal development in humans have been studied but little is known about the molecular events essential for the formation of the retina. To gain further insights into this process, cDNA microarrays containing 16361 human gene probes were used to measure the gene expression levels in retinas. Of the 16361 genes, 68.7%, 71.4% and 69.7% showed positive hybridiza- tion with cDNAs made from 12—16 week fetal, 22—26 week fetal and adult retinas. A total of 814 genes showed a mini- mum of 3-fold changes between the lowest and highest ex- pression levels among three time points and among them, 106 genes had expression levels with the hybridization intensity above 100 at one or more time points. The clustering analysis suggested that the majority of differentially expressed genes were down-regulated during the retinal development. The differentially expressed genes were further classified accord- ing to functions of known genes, and were ranked in de- creasing order according to frequency: development, differ- entiation, signal transduction, protein synthesis and transla- tion, metabolism, DNA binding and transcription, DNA syn- thesis-repair-recombination, immuno-response, ion channel- transport, cell receptor, cytoskeleton, cell cycle, pro-oncogene, stress and apoptosis related genes. Among these 106 differen- tially expressed genes, 60 are already present in NEI retina cDNA or EST Databank but the remaining 46 genes are ab- sent and thus identified as “function unknown”. To validate gene expression data from the microarray, real-time RT-PCR was performed for 46 “function unknown” genes and 6 known retina specific expression genes, and β-actin was used as internal control. Twenty-seven of these genes showed very similar expression profiles between the microarray and real-time RT-PCR data. In situ hybridization revealed both expression level and cellular distribution of NNAT in retina. Finally, the chromosomal locations of 106 differentially ex- pressed genes were also searched and one of these genes is associated with autosomal dominant cone or cone-rod dys- trophy. The data from present study provide insights into understanding genetic programs during human retinal de- velopment and help identify additional retinal disease genes.展开更多
By using the generalized Hadamard product, difference matrix and projection matrices, we present a class of orthogonal projection matrices and related orthogonal arrays of strength two. A new class of orthogonal array...By using the generalized Hadamard product, difference matrix and projection matrices, we present a class of orthogonal projection matrices and related orthogonal arrays of strength two. A new class of orthogonal arrays are constructed.展开更多
In this paper, a family of non-monomial permutations over the finite field F2n with differential uniformity at most 6 is proposed, where n is a positive integer. The algebraic degree of these functions is also determi...In this paper, a family of non-monomial permutations over the finite field F2n with differential uniformity at most 6 is proposed, where n is a positive integer. The algebraic degree of these functions is also determined.展开更多
High-temperature (150-220 ℃) growth leads to the formation of some peptide nanotube/microtube (NT/MT) arrays but the NTs/MTs exhibit closed ends, irreversible phase modification and eliminations of piezoelectric ...High-temperature (150-220 ℃) growth leads to the formation of some peptide nanotube/microtube (NT/MT) arrays but the NTs/MTs exhibit closed ends, irreversible phase modification and eliminations of piezoelectric and hydrophilic properties. Here we demonstrate the fabrication of unidirectionally aligned and stable diphenylalanine NT/MT arrays with centimeter scale area at room temperature by utilizing an external electric field. The interactions between the applied electric field and dipolar electric field on the NTs and surface positive charges are responsible for the formation. The unidirectionally aligned MT array exhibits a supercapacitance of 1,000 μF·cm^-2 at a scanning rate of 50 mV·s^-1; this is much larger than the values reported previously in peptide NT/MT arrays.展开更多
It is shown how the extended Routh array (ERA), which naturally extends the Routh array to the complex case, can handle the appearance of vanishing leading array elements, and how after minor modifications it can be u...It is shown how the extended Routh array (ERA), which naturally extends the Routh array to the complex case, can handle the appearance of vanishing leading array elements, and how after minor modifications it can be used to test the stability in the wide sense of systems of differential equations with complex coefficients and of arbitrary dimension. The recent result advanced for the asymptotic or strict sense stability of such systems falls out as a special case.展开更多
文摘The reorientation of microtubules (MTS) in roots of Oryza sativa L. treated with 2,4_D was studied using confocal laser scanning microscopy. In the control (the roots were not treated with 2,4_D), different distribution patterns of MTS were observed in the different growth zones of root tips. MTS of the cortical cells were randomly aligned in the zone of cell division. They were transversely arranged in the cortical cells of the zone of cell elongation; and obliquely oriented in the root hair zone. After treatment with 2,4_D, MTS displayed distinct changes with reorientation in the cortex of the root tip coupling with the inhibition of root growth. MTS changed their orientation in the cortical cells of the zone of cell division from being randomly oriented to transversely oriented when incubated in 1 mg/L 2,4_D for 1 h. However, they were recovered and became randomly oriented when the roots were treated with 1 mg/L 2,4_D for 24 h. An array of MTS, which was different from that in the control, was observed in the cortical cells of the zone of cell elongation in the roots treated with 1 mg/L 2,4_D for 1 h. After treatment with 10 mg/L 2,4_D for 1 h, the MTS in the cortical cells of the zone of cell division became transversely reorientated, but the pattern of MTS distribution was different from that in the 1_hour treatment of 1 mg/L 2,4_D. MTS were hardly detected in the same type of cells when roots were submerged in 10 mg/L 2,4_D for 24 h. When roots were incubated in 10 mg/L 2,4_D for 1 h, the MTS of the cortical cells in the zone of cell elongation became randomly oriented, and much more randomly when the roots were treated for 24 h.
基金This work was supported by the National Natural Science Foundation of China (No.20934004 and No.91127046) and the National Basic Research Program of China (No.2012CB821500 and No.2010CB934500).
文摘We conducted experiments on specially designed microfluidic chips that generate droplets through a microfluidic ow-focusing approach. The fluid flow in the microfluidic channel produced a shear flow field at low Reynolds numbers. The droplets in the microfluidic system exhibited special droplet pattern formations similar to periodic crystal-like lattices because of the competition between shear forces and surface tension. By adjusting the flow rate ratio of the water (droplet phase) to oil (continuous phase) phases and changing the outlet channel widths, the droplets formed monolayer dispersion to double-layer formation to monolayer squeezing when the outlet channel widths were 250 or 300 μm. We also obtained droplets with monolayer dispersion, three-layer arrangements, double-layer squeezing, and monolayer squeezing when the outlet channel width was 350 μm. The outlet channel width was increased to 400 μm, and four-layer arrangements were observed. We also studied the translation of droplet formation, which resulted in a detailed strategy to control drop size and droplet pattern formation for emulsi cation in microfluidic devices. We expect that our strategy can provide theoretical guidance to synthesize dispersion or polydisperse colloid particles.
文摘To improve the sensitivity of protein microarray, a prism surface replaces the surface of the common microscope slide.The protein targets arrayed on the surface are hybridized and labelled by fluorescent probes. Evanescent excitation occurs when the convergent laser reaches the surface, and a photomultiplier tube detects the emitted fluorescent signal. A two-dimensional actuator scans the whole surface to achieve planar laser excitation and fluorescence collection. The penetration depth of the evanescent field into the protein targets is only some hundred nanometers and can be controlled by different incident angle of the laser beam, so the undesired background signals are reduced dramatically and the detection sensitivity is improved by a factor of 50 to 100 comparing to confocal excitation. This approach can detect low abundance analytes without signal amplification.
基金Supported by the Science and Technology Project of Tianjin(Grant No.12ZCZDSF02200)the Innovation Service Platform Project of Desalination and Comprehensive Utilization(Grant No.CXSF2014-34-C)
文摘Poly(vinylidene fluoride)(PVDF) is a semi-crystalline thermoplastic polymer with excellent thermal stability,electrochemical stability and corrosion resistance, which has been widely studied and applied in industrial nonmetallic heat exchanger and piezoelectric-film sensor. In this study, polyaniline(PANI) nanofibers were synthesized using dodecylbenzene sulfonic acid as the surfactant. The obtained PANI nanofibers were blended in PVDF matrix to enhance thermal conductivity and tensile strength of composite materials. Electric field was applied for the orientation of membrane structure during membrane formation. Scanning electron microscope(SEM) images exhibited that the PANI nanofibers were well-dispersed in the composite membranes. The structure of composite membranes was more orderly after alignment. X-ray diffraction(XRD) and differential scanning calorimetry(DSC) indicated that the content of PANI nanofibers contributed to the transformation of PVDF from α-phase to β-phase. Both the tensile strength and thermal conductivity of composite membranes were significantly improved. This tendency was further enhanced by the application of electric field. The maximum tensile strength was obtained when the content of PANI nanofibers was 3 wt%, which was 46.44% higher than that of pure PVDF membrane. The maximum thermal conductivity of composite membranes after alignment was 84.5% greater than that of pure PVDF membrane when the content of PANI nanofibers was 50 wt%. The composite membrane is a promising new potential material in heat transfer field and the mechanism explored in this study would be informative for further development of similar thermal conductive polymeric materials.
基金National Natural Science Foundation ofChina(No.60573031)Foundation of Na-tional Laboratory for Modern Communica-tions(No.51436060205JW0305)Founda-tion of Senior Visiting Scholarship of Fu-dan University
文摘Substitution permutation network (SPN) is one important structure of block cipher cryptosystems. Prior work has shown different fault analyses on SPN. The formalization of fault analysis of both attack and protect on SPN have been given. The overhead and time tolerance of fault detection have been discussed. The pseudo-blinding method to detect fault attack is introduced, and the balance of the security, overhead and time tolerance based on the evaluation could be made.
基金Project(41672258) supported by the National Natural Science Foundation of ChinaProject(2018045) supported by the Land and Resources Science&Technology Project of Jiangsu Province,China。
文摘The landslide disaster caused by the argillaceous interlayer not only destroys buildings,cultivated land,and roads but also seriously endangers human life and safety.This study concerns the mineral composition of selected argillaceous interlayer and their strength characteristics.To study the mineral composition of argillaceous interlayers,8 kinds of samples in the southern Jiangsu region of China were analyzed utilizing X-ray diffraction(XRD).The repeated direct shear strength tests(RDST)were carried out on the undisturbed specimens of the argillaceous interlayer.The results show that the argillaceous interlayer with high content of kaolinite shows ductile failure mode,which means that there is no obvious residual strength in the shear process.The arrangement of mineral particles on the shear surface of the specimens after different shear displacements was observed under the scanning electron microscope(SEM).It was observed that mineral particles on the shear surface showed a more directional arrangement with the increase of shear displacement.Furthermore,the influence of shear direction on the argillaceous interlayer with completely oriented mineral particles was studied through numerical experiments with four shear strength mechanisms proposition proposed.The influence of the mineral arrangement on the action occasion and magnitude of dilatancy component of shear strength is clarified in the shear mechanism.
文摘The combustion characteristics and particulate emission during combustion of Thai lignite with 30% of secondary air to total air (SA:TA) in a fixed bed combustor have been investigated in real-time. The results have shown that particle formation is governed by competing reaction between the formation of the nucleated sized-particles (Dp 〈 0.1μm) and the coagulated particle (Dp 0.1-1 μm). Temperature and burning rate are the highest priority factors to control the emission of particulate. Furthermore, the co-firing of coal/rice husks at 60:40% mass fraction with 10%SA:TA could be the alternative options to further reduction of particulate and to be recommended.
文摘Retina is a multilayer and highly specialized tissue important in converting light into neural signals. In humans, the critical period for the formation of complex multiplayer structure takes place during embryogenesis be- tween 12 and 28 weeks. The morphologic changes during retinal development in humans have been studied but little is known about the molecular events essential for the formation of the retina. To gain further insights into this process, cDNA microarrays containing 16361 human gene probes were used to measure the gene expression levels in retinas. Of the 16361 genes, 68.7%, 71.4% and 69.7% showed positive hybridiza- tion with cDNAs made from 12—16 week fetal, 22—26 week fetal and adult retinas. A total of 814 genes showed a mini- mum of 3-fold changes between the lowest and highest ex- pression levels among three time points and among them, 106 genes had expression levels with the hybridization intensity above 100 at one or more time points. The clustering analysis suggested that the majority of differentially expressed genes were down-regulated during the retinal development. The differentially expressed genes were further classified accord- ing to functions of known genes, and were ranked in de- creasing order according to frequency: development, differ- entiation, signal transduction, protein synthesis and transla- tion, metabolism, DNA binding and transcription, DNA syn- thesis-repair-recombination, immuno-response, ion channel- transport, cell receptor, cytoskeleton, cell cycle, pro-oncogene, stress and apoptosis related genes. Among these 106 differen- tially expressed genes, 60 are already present in NEI retina cDNA or EST Databank but the remaining 46 genes are ab- sent and thus identified as “function unknown”. To validate gene expression data from the microarray, real-time RT-PCR was performed for 46 “function unknown” genes and 6 known retina specific expression genes, and β-actin was used as internal control. Twenty-seven of these genes showed very similar expression profiles between the microarray and real-time RT-PCR data. In situ hybridization revealed both expression level and cellular distribution of NNAT in retina. Finally, the chromosomal locations of 106 differentially ex- pressed genes were also searched and one of these genes is associated with autosomal dominant cone or cone-rod dys- trophy. The data from present study provide insights into understanding genetic programs during human retinal de- velopment and help identify additional retinal disease genes.
基金The research is supported by the National Natural Science Foundation of China under Grant No. 10571045University Backbone Teachers Foundation of the Education Department of Henan ProvinceNatural Science Foundation of Henan Province under Grant No. 0411011100.
文摘By using the generalized Hadamard product, difference matrix and projection matrices, we present a class of orthogonal projection matrices and related orthogonal arrays of strength two. A new class of orthogonal arrays are constructed.
基金supported by the National Science Foundation of China under Grant Nos.11401172 and 61672212
文摘In this paper, a family of non-monomial permutations over the finite field F2n with differential uniformity at most 6 is proposed, where n is a positive integer. The algebraic degree of these functions is also determined.
基金Acknowledgements This work was supported by the National Basic Research Programs of China under Grants Nos. 2011CB922102 and 2013CB932901 and the National Natural Science Foundation of China (Nos. 11374141 and 21203098 ).
文摘High-temperature (150-220 ℃) growth leads to the formation of some peptide nanotube/microtube (NT/MT) arrays but the NTs/MTs exhibit closed ends, irreversible phase modification and eliminations of piezoelectric and hydrophilic properties. Here we demonstrate the fabrication of unidirectionally aligned and stable diphenylalanine NT/MT arrays with centimeter scale area at room temperature by utilizing an external electric field. The interactions between the applied electric field and dipolar electric field on the NTs and surface positive charges are responsible for the formation. The unidirectionally aligned MT array exhibits a supercapacitance of 1,000 μF·cm^-2 at a scanning rate of 50 mV·s^-1; this is much larger than the values reported previously in peptide NT/MT arrays.
文摘It is shown how the extended Routh array (ERA), which naturally extends the Routh array to the complex case, can handle the appearance of vanishing leading array elements, and how after minor modifications it can be used to test the stability in the wide sense of systems of differential equations with complex coefficients and of arbitrary dimension. The recent result advanced for the asymptotic or strict sense stability of such systems falls out as a special case.