微波辅助水提联用泡沫分离富集皂荚皂苷的工艺研究

为优化微波辅助水提联用泡沫分离富集皂荚(Gleditsia sinensis Lam.)皂苷提取条件,通过单因素试验和响应面试验,以微波功率、微波时间、液料比、原料粒径为试验因素探究微波辅助水提取的理想提取条件,再进一步以富集比为考察因素探究气体流速、温度、装液量、进料液浓度对富集皂荚皂苷的影响。结果表明:微波辅助水提法的优化条件为微波时间33 s,微波功率1000 W,原料粒径100目,液料比35 mL/g。泡沫分离富集法的优化富集条件为气体流速400 mL/min,装液量30 mL,进料液浓度7.13 mg/mL,温度70℃。微波辅助水提联用泡沫分离富集皂荚皂苷,相比于传统醇提以及水煎法有提取效率高,绿色环保且设备简单的优点。

水提法和微波助提法在蛹虫草多糖提取中的应用

目的:探讨蛹虫草菌丝体多糖提取方法的应用。方法:利用水提法和微波助提法提取蛹虫草菌丝体中多糖成分,利用苯酚-硫酸法测定其多糖的含量。结果:水提法粗多糖的提取率为22.26%,微波助提法的提取率为15.16%。结论:菌丝体粗多糖提取过程中应用传统水提法提取率高,但其工艺复杂、耗时,微波助提法工艺简便、快速。

微波浸提法提取大枣多糖的工艺研究

[目的]优化微波浸提法提取大枣多糖的最佳工艺条件。[方法]采用微波浸提法提取大枣多糖,初步考察了料液比、微波功率、微波处理时间对大枣多糖提取率的影响。[结果]微波浸提法提取大枣多糖的最佳工艺条件为:料液比1∶50 g/ml、微波功率420 W、微波处理时间8 min,在此工艺条件下提取率达到7.99%。[结论]微波辅助工艺在一定程度上提高了大枣多糖的提取率,可为进一步开发利用大枣资源提供参考依据。

微波辅助浸提法提取野生龙葵果红色素的工艺筛选

以野生龙葵果实为原料,采用微波辅助萃取法,对龙葵果红色素的最佳浸提及浸提溶剂条件进行了筛选,结果表明:以1.5%柠檬酸溶液为提取剂,微波火力为中高火,料液比为1∶20,微波处理时间1 m in是微波辅助提取龙葵果红色素的最佳浸提条件。与传统的加热提取和常温提取相比,此法极大地缩短了提取时间,而且提取率也明显提高。

微波压力浸提法测定板蓝根总酸度

介绍了一种微波浸提板蓝根、快速测定其总酸度的新方法。分析了微波功率、微波时间、料液比等因素对测定结果的影响,与经典的分析方法相对照,没有显著性差异,结果令人满意。该法操作简便、省时、省事、节约能源、结果准确可靠、测定速度快,测定效率高,宜于批量样品的测定,为板蓝根总酸度的测定提供了一个快速、准确、便捷的分析方法。

微波预处理水提法提取甘孜虎掌菌多糖的工艺优化

为更好地开发利用四川甘孜州野生虎掌菌,对比传统水提法与微波预处理水提法提取虎掌菌多糖的最佳工艺,以多糖得率为考察指标,采用正交试验分别设计L9(34)和L16(45)得到最佳的工艺条件。结果表明:提取虎掌菌多糖的最佳方法为微波预处理水提法,在微波预处理时间70s、乙醇浓度100%、微波功率550 W、浸提时间2h、料液比为1∶20的条件下,甘孜虎掌菌中粗多糖得率为15.56%,是传统水提法的2.04倍。

玉米须总皂苷提取方法比较研究

以玉米须为原料,对玉米须总皂苷的提取方法进行研究。考察有机试剂浸提法、超声法、微波法对玉米须总皂苷提取率的影响,并对这三种方法的动力学特性进行对比。试验结果表明:有机溶剂浸提法提取玉米须总皂苷得率较高,但有机溶剂用量较大,实验时间较长;微波法和超声法试验相对简单,时间较短、温度较低、提取速率较快。因此,微波法和超声法作为玉米须总皂苷的提取方法值得推广。

蛹虫草虫草素提取工艺的研究

以蛹虫草[Cordyceps militaris(Linn.)Link]子实体为材料,首先通过单因素分析法探索虫草素的最佳提取溶剂,结果表明,70%乙醇是虫草素的最佳提取溶剂,并通过正交试验考察了微波助提法中微波功率、微波时间、提取次数和料液比4个因素对虫草素提取功率的影响,最终建立了提取虫草素的最佳工艺条件,即微波功率350 W,微波处理时间4 min,提取2次,料液比1∶50(g∶m L),提取率可达6.87%。

芦笋老茎中多糖提取工艺的优化

[目的]优化微波直提法和微波-索氏提取法提取芦笋老茎中多糖的工艺。[方法]以多糖提取率为考察指标,通过正交试验优化2种提取方法的提取工艺。[结果]微波直提法的最佳工艺条件为:料液比为1∶30,提取时间为20 min,提取温度为60℃,微波功率为600 W,在此条件下多糖的提取率为4.35%;微波-索氏提取法的最佳工艺条件为:料液比为1∶33,微波时间为10 min,微波功率为600W,提取温度为70℃,在此条件下多糖的提取率为2.29%。[结论]微波直提法具有提取效率高,提取温度低,能量消耗小等特点,可用于实际生产中。

野生和栽培马齿苋黄酮含量的比较研究

采用微波辅助乙醇浸提的方法提取马齿苋黄酮,并通过正交试验确定了最佳提取条件。结果表明,微波辅助提取的影响因素顺序为:料液比>微波处理时间>乙醇浓度>微波强度;马齿苋黄酮微波辅助乙醇浸提的最佳条件为:料液比1:50(g/ml)、微波处理时间120s、乙醇浓度70%、微波强度为中高火档(648W)。提取得到野生型和栽培型马齿苋黄酮含量分别为:5.432%和5.506%。

Study on Extraction Process of Polysaccharides from Chrysanthemum

[Objective] The aim was to study the optimum extraction process of polysaccharide from chrysanthemum.[Method] By dint of orthogonal and single-factor experiments,the influences of microwave efficiency,extraction time,and liquid ratio on the extraction rate of polysaccharides from chrysanthemum were studied.The optimum operation condition by microwave extraction method was determined and was compared with that by traditional extraction method.[Result] The optimum extraction conditions were:800 W,1:15(solid-liquid ratio),and 15 min.The polysaccharides extraction yield under such optimum condition was 5.59%.The extraction yield of impregnation was generally about 2.73%.[Conclusion] Compared with the traditional extraction method,the microwave extraction was simple,convenient,energy saving,and efficient,and was suitable for industrial extraction of polysaccharide.

Optimization of the microwave-assisted extraction of phlorotannins from Saccharina japonica Aresch and evaluation of the inhibitory effects of phlorotannin-containing extracts on HepG2 cancer cells

The use of a microwave-assisted extraction （MAE） method for the extraction ofphlorotannins from Saccharinajaponica Aresch （S.japonica） has been evaluated with particular emphasis on the influential parameters, including the ethanol concentration, solid/liquid ratio, extraction time, extraction temperature, and microwave power. The MAE procedure was optimized using single-factor design and orthogonal array design （OAD）. The content of total phlorotannins in S. japonica was determined using a Folin-Ciocalteu （FC） assay. A maximum total phlorotannin content of 0.644 mg of phloroglucinol equivalent per gram of dry weight plant （mg PGE/g DW） was obtained using the optimized model, which included an ethanol concentration of 55%, solid/liquid ratio of 1：8, extraction time of 25 min, irradiation power of 400 W, and temperature of 60~C. Under similar conditions, the application of a conventional extraction method led to a lower phlorotarmin yield of 0.585 mg PGE/g WD. These results demonstrated that the MAE approach provided better results for the extraction ofphlorotarmins from S.japonica and was a promising technique for the extraction of phenolic compounds from S. japonica and other materials. In addition, screening tests for the inhibitory activity showed that the phlorotannin-containing extracts significantly inhibited the growth of human hepatocellular carcinoma cells （HepG2） by inducing their apoptosis. The morphological changes that occurred during cell apoptosis were characterized using Hoechst33258 staining.

五角枫叶绿素的提取研究

采用微波助提法提取五角枫叶绿素,所得最佳提取条件如下:提取时间120 s、料液比1∶10、提取液pH值为7时,提取效果较好,提取完全、误差小、效率高。

茶薪菇菌丝体多糖提取方法的研究

茶薪菇(又名茶树菇)是一种食药用价值很高的真菌，不仅食味独特，营养丰富，而且具有抗衰老、降低胆固醇、防癌和抗癌等特殊功效。目前，来源于食药用多糖的部分产品已进入临床，用于癌症等疾病的免疫治疗．而茶薪菇多糖的研究目前国内尚未见报道。本文通过正交试验等方法研究了茶薪菇菌丝体多糖提取方法，即热水浸提法、复合酶解法、微波辅助水浸提法等，

The Influence of Drying Temperature and Extraction Methods on a-Mangostin in Mangosteen Pericarp

The effects of drying temperature and extraction methods on α-mangostin content in mangosteen pericarp （Garcinia mangostana L.） powder were investigated. In the first part of experiment suitable drying temperature for retention α-mangostin content was determined. Three levels of drying temperatures （55, 65 and 75 ℃） were used in this study. The drying rates were increased with drying temperature. Room temperature extraction method was performed to investigate the effect of drying temperature on retention α-mangostin content in mangosteen pericarp. The α-mangostin content extracted at three different drying temperatures （55, 65 and 75 ℃） was 35.98 ± 0.49%, 40.32 ± 0.24%, and 37.79±0.34% w/w, respectively. The results showed that the suitable temperature for drying mangosteen pericarp was 65 ℃ that gave the highest of α-mangostin content. The second part of experiment was the comparison between extraction methods, such as shaking water bath extraction （SWE）, soxhlet extraction （SE） and microwave-assisted extraction （MAE）. The results show that MAE gave the highest extraction rate and α-mangostin content as compare to SWE and SE. The α-mangostin content extraction from SWE, SE and MAE are 45.83 ± 0.02, 34.82± 0.17 and 49.79 ± 0.15% w/w of crude extract, respectively.

A Comparative Study of Process Parameter Optimization and Process of Flavonoid in Planted Trollius Chinensis through Backflow Method, Ultrasonic Wave Method and MicrowaveMethod

It takes flavonoid extraction rate as indicator and adopts the method of orthogonal experiment to study process of total flavonoid in Trollius chinensis through ultrasonic wave assisted extraction, microwave assisted extraction and backflow extraction, as well as optimization of process parameter. The result indicates that in terms of extraction efficiency, microwave extraction method is better than ultrasonic radiation extraction method, which is better than backflow extraction method. Optimal process parameter through backflow extraction is： backflow temperature 60℃, fluid material ratio 1：20, ethanol density 65%, backflow time 60min; optimal process parameter through ultrasonic wave assisted extraction is： ultrasonic radiation temperature 70℃, fluid material ratio 1：20, ethanol density 65%, ultrasonic wave radiation time 45rain; optimal process parameter of microwave assisted extraction is： microwave radiation temperature 60℃, fluid material ratio 1：20, ethanol density 75%, microwave radiation time 45min.

银杏叶中有效成分的微波提取及抗氧化活性研究

用正交试验设计优选出银杏叶黄酮类化合物的微波提取工艺,并采用化学发光法对银杏叶的粗提物、正丁醇萃取物及其余水层清除自由基和抗氧化性能进行了研究.结果表明:当固液比(m/m)为1∶30,微波中档400 W浸提时间为1 h,然后70℃水浴浸提1 h,黄酮的提取率最高.3种提取物均有较强的抗氧化活性.其中粗提物的抗氧化活性比正丁醇萃取物好,同时发现萃取后的水层部分虽然黄酮类化合物含量很低,但也有较强的抗氧化活性.证明银杏叶提取物是一组复杂的、而又有相互协同增效作用的混合物,进一步分离纯化可能对提高其抗氧化活性作用不显著.