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微流控多层纸芯片研究乳腺癌组织的微环境酸化 被引量:7
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作者 严伟 张琼 +4 位作者 陈斌 梁广铁 李伟萱 周小棉 刘大渔 《分析化学》 SCIE EI CAS CSCD 北大核心 2013年第6期822-827,共6页
将多层纸芯片技术用于肿瘤微环境酸化研究。将种植有乳腺癌细胞的8层硝酸纤维素薄膜叠放并封装于芯片中,用以模拟3D乳腺癌组织。灌流培养多层纸芯片乳腺癌组织数天后拆分多层纸芯片,以检测各层薄膜上细胞生存、增殖和胞内乳酸含量,解析... 将多层纸芯片技术用于肿瘤微环境酸化研究。将种植有乳腺癌细胞的8层硝酸纤维素薄膜叠放并封装于芯片中,用以模拟3D乳腺癌组织。灌流培养多层纸芯片乳腺癌组织数天后拆分多层纸芯片,以检测各层薄膜上细胞生存、增殖和胞内乳酸含量,解析不同深度下肿瘤细胞微环境酸化程度。实验表明,细胞酸化程度受灌流速度影响,高灌流速度可以增加纸层上细胞密度,酸性代谢产物排出增加。缺氧也是导致微环境酸化的重要因素。随着氧气扩散距离的增加,酸化程度加重,并且肿瘤细胞生存率和增殖率相应降低。 展开更多
关键词 流控芯片 乳腺癌 微环境酸化 三维细胞培养 纸芯片细胞培养
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Analyses of concrete microcosmic structure in multi-media environment
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作者 闫波 姜安玺 +2 位作者 王幼青 刘丽艳 徐桂芹 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2004年第3期286-290,共5页
The structure of concrete generally serves in multi-media environments; various environments act differently on concrete. The compound action of some severe environments will threaten the duration of concrete and decr... The structure of concrete generally serves in multi-media environments; various environments act differently on concrete. The compound action of some severe environments will threaten the duration of concrete and decrease the service life of a concrete structure if improperly handled. In this paper the microstructure of concrete is observed by using Scanned Electric Microscope (SEM) through contrasting experiments in media of acid, alkali and salt with that of freezing-thawing in the same medium environment. This study is to supply a certain basis for changing traditional thinking of mechanical design and to combine construction reliability design with durability of concrete design. 展开更多
关键词 medium environment freezing-thawing acid erosion freezing-(thawing) alkali erosion freezing-thawing salt erosion alternate freezing-thawing microcosmic structure
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