微生物动力学优化算法

为了解决一类函数优化问题,利用带时滞影响的混杂食物链微生物培养动力学理论提出一种微生物动力学优化(MDO)算法。在该算法中,假设有多个微生物种群在一个培养系统中培养,微生物种群的生长不但受注入到培养系统中的培养液流量、营养物质和有害物质的浓度影响,而且受种群之间相互作用的影响;定期注入的培养液会突然增加营养物质和有毒物质的浓度,从而会突然加大对种群的影响。利用上述特点构造出了吸收算子、攫取算子、混杂算子和毒素算子;利用这些算子和种群的生长变化,能够快速求解优化问题的全局最优解。仿真实验结果表明,MDO算法对求解维数较高的优化问题具有一定的优势。

口服制剂车间生产污水处理工艺的优化研究

以口服制剂车间实际生产污水为研究对象进行实验,通过对污水进行化学需氧量C O D和原料药A P I残留量的检测,相对高浓污水可直接排入低浓水池进行处理,改变了以前口服剂车间污水先收集稀释再排放的传统处理方式。实验结果显示,车间污水经一氧化池处理后,COD含量明显降低,经后续处理后完全可以达到市政排放要求;API含量已降到市政要求标准限值之下,后续处理可使A P I含量持续降低直至检测不出。研究可为口服车间污水排放系统设计、减少污水处理成本提供参考。

Screening of One Microbial Flocculant for Treatment of Methyl Orange Wastewater and its Decolorization Effect

[Objective] To screen out a microbial flocculant with good decolorization effect on methyl orange wastewater,and study the effect of different cultural conditions on decolorization effect of methyl orange.[Method] Absorbance of methyl orange solution before and after decolorization was determined by spectrophotometer,and the decolorization rate was calculated to compare the effects of different cultural conditions on removal rate of methyl orange.[Result]An optimal actinomycete stain（F-1-2） was screened out,and the best cultural condition was as follows：with sucrose as carbon source and NaNO3 as nitrogen source,cultured in constant temperature oscillator at 150 r/min,30℃ for 72 h.Under the optimal condition,the removal rate against methyl orange could reach 68.4%.[Conclusion]Different culture conditions have great impact on decolorization effect of strain.

Optimization of Cr(VI) bioremediation in contaminated soil using indigenous bacteria

Bench-scale soil column experiments were carried out to evaluate the effectiveness of Cr（VI） bioremediation process in soils by using indigenous bacteria with the addition of bacteria nutrient media. Effects of particle size, spray intensity, initial Cr（VI） concentration, circulation mode and soil depth on Cr（VI） remediation were studied. Results show that soils after 6 d remediation with spray intensity controlled in the range of 29.6-59.2 mL/min could well fulfill the requirement of concrete aggregate and roadbed material usage, for the leaching toxicity concentration of the Cr（VI） in treated soils under the chosen condition is far less than 5 mg/L The leaching toxicity and fractions of both hexavalent chromium and trivalent chromium from remediated soils were determined and compared with that of untreated soil. The results show that water soluble Cr（VI） declines from 1520.54 mg/kg to 0.68 mg/kg, exchangeable Cr（VI） decreases from 34.83 mg/kg to 0.01 mg/kg and carbonates-bonded Cr（V1） falls from 13.55 mg/kg to 0.68 mg/kg. Meanwhile, a corresponding increase in carbonate-bonded Cr（III）, Fe and Mn oxides-bonded Cr（III） and organic matter-bonded Cr（III） are found. It reveals that indigenous bacteria can leach out water soluble Cr（VI）, exchangeable Cr（VI） and carbonates-bonded Cr（VI） from contaminated soil followed by converting into carbonate-bonded Cr（III）, Fe and Mn oxides-bonded Cr（IlI）, organic matter-bonded Cr（III） and residual Cr（III）.

Remote sensing Extraction model of redtide biomass by airborne hyperspectral technique

Our work is based on the known research results of inherent optical quality of ocean color constituents.According to optimized parameters and induced fluorescence term of chlorophyll, this paper puts forward a remote sensing reflectance model of sea water, which is fitted in Liaodong Bay of Bohai. An inverse model that can evaluate redtide biomass according to chlorophyll retrieval is provided by inducing a functional extreme problem. The calculation example of the model indicates that the inversion model has explicit mathematic and physical meaning, but its practicability needs to be verified.

Study on Optimal Fermentation and Storage Time of Soybean Food （Thua-Nao）in Thailand

The soybean cultivar Tadang Muangpai was used to improve the productive quality of Thua-Nao and reduce the concentration of aflatoxin to less than 20 ppb. It was conducted at CMFCRC, Chiangmai, Thailand between Dec. 2006 and Mar. 2007. Soybean was boiled for 5 hours and then fermented at different time to create natural bacterial species, mainly Bacillus spp. Thua-Nao could be stored up to 90 days after in storage. Nutritional value, food value, and microorganisms content were investigated during fermentation and storage. Also, aflatoxin content of Thua-Nao was recorded during storage. The results showed that 3 days of soybean fermentation gave the best performance of Thua-Nao in term of nutritional value （protein = 47.12%）, food value, and content of Bacillus spp. （2.78 × 10^9 CFU/g）. Without being harmed from aflatoxin, Thua-Nao could be stored not more than 23 days in normal room （Tmax. = 33.9 ℃, Tmin. = 15.8 ℃） and not more than 36 days in climate-controlled room （Tmax. = 20 ℃, Tmin. = 15 ℃）.

一株新海洋粘质沙雷氏菌产灵菌红素发酵条件优化

灵菌红素是由微生物产生的一种红色次级代谢产物,因其具有抗菌、抗癌和抗疟疾等功效,受到了生物医药领域的广泛关注。微生物发酵是当前产灵菌红素的主要方法,分离筛选高产灵菌红素的微生物、优化发酵条件是提高灵菌红素产率的重要途径。本研究从深圳湾筛选出一株含有红色色素的菌株,并基于16S rRNA基因序列对该菌株进行了系统发育分析和物种鉴定。紫外可见光全波长扫描和HPLC-MS图谱分析证明,该菌株所产红色色素为灵菌红素。进一步通过单因素试验和正交优化法优化了该菌产灵菌红素的发酵条件和发酵培养基组分。系统发育分析表明,该菌株为一株海洋粘质沙雷氏菌(Serratia marcescens),并将其命名为S.marcescens SOCE 001。产灵菌红素的最佳发酵条件为:温度28℃、振荡培养转速220r/min、培养基pH 7。发酵培养基最佳组合为:果糖添加量2 g/L、蛋白胨添加量10 g/L,MgSO_(4)添加量2 g/L。优化发酵条件后,经24 h培养,灵菌红素产量可达2.468 g/L。

高效COD消解菌DZG-F1在海南省高位池养殖水质管理中的应用潜力

从海南省高位池养殖水中分离出一株高效消解化学需氧量COD (Chemical Oxygen Demand)的菌株DZG-F1,经16SrDNA序列鉴定,初步鉴定为芽孢杆菌属(Bacillus),命名为Bacillus sp.DZG-F1,在淀粉7%、pH 7.0、温度35℃、K2HPO4添加量3‰的培养条件下,能有效促进该菌株生长;微生物固定材料中固菌能力为改性椰丝纤维>海藻酸钠微球>椰丝纤维,其中改性椰丝纤维固定后的菌株在水产养殖废水处理中COD消解能力最佳,其48 h COD消解率为50.75%。本研究表明,固定化DZG-F1菌株对海水养殖废水中的COD具有良好的消解能力,在海水养殖水质管理中具有较大的应用潜力。