Understanding microbial growth is essential to any research conducted in the fields of microbiology and biotechnology. Current methods of determining growth characteristics of microbes involve subjective graphical int...Understanding microbial growth is essential to any research conducted in the fields of microbiology and biotechnology. Current methods of determining growth characteristics of microbes involve subjective graphical interpretations of linearized logarithmic data. Reducing error in logistical data decreases disparity between graphical and analytical predictions of microbial characteristics. In this study, a method has been developed to calculate the kinetics of microbial characteristics utilizing a modified Maclaurin series. Convergence of this series approaches the true kinetic value of microbial characteristics to include specific growth rates. In this research, a modified Maclaurin series is used to evaluate microbial kinetics in comparison to graphical determinations.展开更多
The possibility of producing biogas and methane from two phases olive pomace was considered using anaerobic digestion and the microbial characteristic of digestate for the agrarian use was analyzed. In the work, the m...The possibility of producing biogas and methane from two phases olive pomace was considered using anaerobic digestion and the microbial characteristic of digestate for the agrarian use was analyzed. In the work, the main aim was to obtain biogas, made from at least 50% methane, and a digestate that can be used in the field of agronomy, from the anaerobic digestion of the substrates. The tests were carried out by digesting different mixtures of the two-phase pomace, mulberry leaves and mud civil wastewater （pre-digested） in a batch system and in anaerobic mesophilic conditions （35 ~C）. The substrates were properly homogenized in order to obtain mixtures of known and uniform composition. The initial and final STi （Total Solids） and initial SVi （Volatile Solids）, the concentration of chemical oxygen demand and total phenols were measured and the process yield （m3/t SV） was quantified with standard procedure. The objectives of the study were the analysis of microbial biodiversity developed during fermentation of mixtures based products and the microbial communities corresponding to Eubacteria, Archaea and Fungiwas analyzed. The suitability of the digestate for agronomical use was evaluated by estimating pathogens bacteria that may be present and by index of inhibition of plant organisms model.展开更多
Microfluidic technology provides opportunities to create in vitro models with physiological microenvironment for cell study.Introducing the identified key aspects,including tissue-tissue interfaces,spatiotemporal chem...Microfluidic technology provides opportunities to create in vitro models with physiological microenvironment for cell study.Introducing the identified key aspects,including tissue-tissue interfaces,spatiotemporal chemical gradients,and dynamic mechanical forces,of living organs into the microfluidic system,"organs-on-chips"display an unprecedented application potential in a lot of biological fields such as fundamental physiological and pathophysiological research,drug efficacy and toxicity testing,and clinical diagnosis.Here,we review the recent development of organs-on-chips and briefly discuss their future challenges.展开更多
文摘Understanding microbial growth is essential to any research conducted in the fields of microbiology and biotechnology. Current methods of determining growth characteristics of microbes involve subjective graphical interpretations of linearized logarithmic data. Reducing error in logistical data decreases disparity between graphical and analytical predictions of microbial characteristics. In this study, a method has been developed to calculate the kinetics of microbial characteristics utilizing a modified Maclaurin series. Convergence of this series approaches the true kinetic value of microbial characteristics to include specific growth rates. In this research, a modified Maclaurin series is used to evaluate microbial kinetics in comparison to graphical determinations.
文摘The possibility of producing biogas and methane from two phases olive pomace was considered using anaerobic digestion and the microbial characteristic of digestate for the agrarian use was analyzed. In the work, the main aim was to obtain biogas, made from at least 50% methane, and a digestate that can be used in the field of agronomy, from the anaerobic digestion of the substrates. The tests were carried out by digesting different mixtures of the two-phase pomace, mulberry leaves and mud civil wastewater （pre-digested） in a batch system and in anaerobic mesophilic conditions （35 ~C）. The substrates were properly homogenized in order to obtain mixtures of known and uniform composition. The initial and final STi （Total Solids） and initial SVi （Volatile Solids）, the concentration of chemical oxygen demand and total phenols were measured and the process yield （m3/t SV） was quantified with standard procedure. The objectives of the study were the analysis of microbial biodiversity developed during fermentation of mixtures based products and the microbial communities corresponding to Eubacteria, Archaea and Fungiwas analyzed. The suitability of the digestate for agronomical use was evaluated by estimating pathogens bacteria that may be present and by index of inhibition of plant organisms model.
基金supported by the Ministry of Science and Technology(2012AA022703,2012AA030608,2011CB933201 and 2009CB930001)the National Natural Science Foundation of China(31170905,21025520,51073045,31170905,GZ 614 and 91213305)+1 种基金the Chinese Academy of Sciences(KJCX2-YW-M15)the State Major Scientific and Technological Project of China(2013ZX09507005)
文摘Microfluidic technology provides opportunities to create in vitro models with physiological microenvironment for cell study.Introducing the identified key aspects,including tissue-tissue interfaces,spatiotemporal chemical gradients,and dynamic mechanical forces,of living organs into the microfluidic system,"organs-on-chips"display an unprecedented application potential in a lot of biological fields such as fundamental physiological and pathophysiological research,drug efficacy and toxicity testing,and clinical diagnosis.Here,we review the recent development of organs-on-chips and briefly discuss their future challenges.
