The dynamic distribution of phosphorylated Histone H3 on Ser10 (phospho-H3) in cells was investigated to determineits function during mitosis. Human breast adenocarcinoma cells MCF-7, and Chinese hamster cells CHO wer...The dynamic distribution of phosphorylated Histone H3 on Ser10 (phospho-H3) in cells was investigated to determineits function during mitosis. Human breast adenocarcinoma cells MCF-7, and Chinese hamster cells CHO were analyzedby indirect immunofluorescence staining with an antibody against phospho-H3. We found that the phosphorylationbegins at early prophase, and spreads throughout the chromosomes at late prophase. At metaphase, most of the phospho-H3 aggregates at the end of the condensed entity of chromosomes at equatorial plate. During anaphase and telophase,the fluorescent signal of phospho-H3 is detached from chromosomes into cytoplasm. At early anaphase, phospho-H3shows ladder bands between two sets of separated chromosome, and forms “sandwich-like structure” when the chro-mosomes condensed. With the cleavage progressing, the “ladders” of the histone contract into a bigger bright dot. Thenthe histone aggregates and some of compacted microtubules in the midbody region are composed into a “bar-like”complex to separate daughter cells. The daughter cells seal their plasma membrane along with the ends of the “bar”,inside which locates microtubules and modified histones, to finish the cytokinesis and keep the “bar complex” out of thecells. The specific distribution and kinetics of phospho-H3 in cytoplasm suggest that the modified histones may takepart in the formation of midbody and play a crucial role in cytokinesis.展开更多
Presence of fine dust in air causes serious health hazard for mine operators resulting in such serious problems as coal workers’pneumoconiosis and silicosis.Major sources of dust appear of course along the mining fac...Presence of fine dust in air causes serious health hazard for mine operators resulting in such serious problems as coal workers’pneumoconiosis and silicosis.Major sources of dust appear of course along the mining face where the minerals are extracted.Proper control and management are required to ensure safe working environment in the mine.Here,we utilize the computational fluid dynamic(CFD)approach to evaluate various methods used for mitigating dust dispersion from the mining face and for ensuring safe level of dust concentration in the mine tunnel for safety of the operators.The methods used include:application of blowing and exhaust fans,application of brattice and combination of both.The results suggest that among the examined methods,implementation of appropriately located brattice to direct the flow from the main shaft to the mining face is the most effective method to direct dust particles away from the mining face.展开更多
Governing valve is the necessary passage through which steam enters into the steam turbine. The instability of the gas flow inside valve is the main reason that can induce the valve vibration, especially the valve rod...Governing valve is the necessary passage through which steam enters into the steam turbine. The instability of the gas flow inside valve is the main reason that can induce the valve vibration, especially the valve rod vibration. In order to reduce the vibration and improve the performance of the governing valve such as the security and economy of the steam turbine, we try to find the method by experimental investigation. As to commonly used governing valve such as ball governing valve in this paper, a number of micro pressure sensors that have high frequencies and nice dynamic capability are employed successfully. The micro sensors are inserted directly in key positions of the valve, such as positions of valve seat throat, valve disc top and so on. The collection and measurement of many different working conditions are carried out and the conclusion of the valve instability is obtained. Therefore, vibration induced by fluid flow is controlled and reduced by means of regulating operation conditions and valve structure. Meanwhile, by numerical simulation of ball governing valve, valve disc adhered flow and asymmetric collision force are considered as main factor to cause oscillation under the condition of small lift as well as small and middle pressure ratio.展开更多
Entosis, a ceU-in-ceU process, has been implicated in the formation of aneuploidy associated with an aberrant cell division control. Microtubule plus-end-tracking protein TI P150 facilitates the loading of MCAK onto t...Entosis, a ceU-in-ceU process, has been implicated in the formation of aneuploidy associated with an aberrant cell division control. Microtubule plus-end-tracking protein TI P150 facilitates the loading of MCAK onto the microtubule plus ends and orchestrates micro- tubule plus-end dynamics during cell division. Here we show that TIP150 cooperates with MCAK to govern entosis via a regulatory cir- cuitry that involves Aurora A-mediated phosphorylation of MCAK. Our biochemical analyses show that MCAK forms an intra-molecular association, which is essential for TIP150 binding. Interestingly, Aurora A-mediated phosphorylation of MCAK modulates its intra-mo- lecular association, which perturbs the MCAK-TI P150 interaction in vitro and inhibits entosis in vivo. To probe if MCAK-TIP150 inter- action regulates microtubule plasticity to affect the mechanical properties of ceUs during entosis, we used an optical trap to measure the mechanical rigidity of live MCF7 ceils. We find that the MCAK cooperates with TIP150 to promote microtubule dynamics and modulate the mechanical rigidity of the cells during entosis. Our results show that a dynamic interaction of MCAK-TI P150 orchestrated by Aurora A-mediated phosphorylation governs entosis via regulating microtubule plus-end dynamics and cell rigidity. These data reveal a previously unknown mechanism of Aurora A regulation in the control of microtubule plasticity during ceU-in-ceU pro- cesses.展开更多
The large-scale assembly and fabrication method for single-walled carbon nanotube(SWCNT) nano devices was implemented.Assembly of SWCNT field effect transistor(FET) was realized by floating potential dielectrophoresis...The large-scale assembly and fabrication method for single-walled carbon nanotube(SWCNT) nano devices was implemented.Assembly of SWCNT field effect transistor(FET) was realized by floating potential dielectrophoresis approach.The simulation of floating potential distribution of the chip was performed by comsol multiphysics coupling software.Six hundred devices were assembled on the area of less than one square centimeter.The fabricated devices were characterized by atomic force microscopy and scanning electron microscopy.The experimental results showed that large-scale assembly had been realized,and the success rate of ideal assembly for SWCNT FET had been assessed.展开更多
文摘The dynamic distribution of phosphorylated Histone H3 on Ser10 (phospho-H3) in cells was investigated to determineits function during mitosis. Human breast adenocarcinoma cells MCF-7, and Chinese hamster cells CHO were analyzedby indirect immunofluorescence staining with an antibody against phospho-H3. We found that the phosphorylationbegins at early prophase, and spreads throughout the chromosomes at late prophase. At metaphase, most of the phospho-H3 aggregates at the end of the condensed entity of chromosomes at equatorial plate. During anaphase and telophase,the fluorescent signal of phospho-H3 is detached from chromosomes into cytoplasm. At early anaphase, phospho-H3shows ladder bands between two sets of separated chromosome, and forms “sandwich-like structure” when the chro-mosomes condensed. With the cleavage progressing, the “ladders” of the histone contract into a bigger bright dot. Thenthe histone aggregates and some of compacted microtubules in the midbody region are composed into a “bar-like”complex to separate daughter cells. The daughter cells seal their plasma membrane along with the ends of the “bar”,inside which locates microtubules and modified histones, to finish the cytokinesis and keep the “bar complex” out of thecells. The specific distribution and kinetics of phospho-H3 in cytoplasm suggest that the modified histones may takepart in the formation of midbody and play a crucial role in cytokinesis.
基金financially supported by the Singapore Economic Development Board (EDB) through Minerals Metals and Materials Technology Centre (M3TC) (No.R261501013414)
文摘Presence of fine dust in air causes serious health hazard for mine operators resulting in such serious problems as coal workers’pneumoconiosis and silicosis.Major sources of dust appear of course along the mining face where the minerals are extracted.Proper control and management are required to ensure safe working environment in the mine.Here,we utilize the computational fluid dynamic(CFD)approach to evaluate various methods used for mitigating dust dispersion from the mining face and for ensuring safe level of dust concentration in the mine tunnel for safety of the operators.The methods used include:application of blowing and exhaust fans,application of brattice and combination of both.The results suggest that among the examined methods,implementation of appropriately located brattice to direct the flow from the main shaft to the mining face is the most effective method to direct dust particles away from the mining face.
文摘Governing valve is the necessary passage through which steam enters into the steam turbine. The instability of the gas flow inside valve is the main reason that can induce the valve vibration, especially the valve rod vibration. In order to reduce the vibration and improve the performance of the governing valve such as the security and economy of the steam turbine, we try to find the method by experimental investigation. As to commonly used governing valve such as ball governing valve in this paper, a number of micro pressure sensors that have high frequencies and nice dynamic capability are employed successfully. The micro sensors are inserted directly in key positions of the valve, such as positions of valve seat throat, valve disc top and so on. The collection and measurement of many different working conditions are carried out and the conclusion of the valve instability is obtained. Therefore, vibration induced by fluid flow is controlled and reduced by means of regulating operation conditions and valve structure. Meanwhile, by numerical simulation of ball governing valve, valve disc adhered flow and asymmetric collision force are considered as main factor to cause oscillation under the condition of small lift as well as small and middle pressure ratio.
文摘Entosis, a ceU-in-ceU process, has been implicated in the formation of aneuploidy associated with an aberrant cell division control. Microtubule plus-end-tracking protein TI P150 facilitates the loading of MCAK onto the microtubule plus ends and orchestrates micro- tubule plus-end dynamics during cell division. Here we show that TIP150 cooperates with MCAK to govern entosis via a regulatory cir- cuitry that involves Aurora A-mediated phosphorylation of MCAK. Our biochemical analyses show that MCAK forms an intra-molecular association, which is essential for TIP150 binding. Interestingly, Aurora A-mediated phosphorylation of MCAK modulates its intra-mo- lecular association, which perturbs the MCAK-TI P150 interaction in vitro and inhibits entosis in vivo. To probe if MCAK-TIP150 inter- action regulates microtubule plasticity to affect the mechanical properties of ceUs during entosis, we used an optical trap to measure the mechanical rigidity of live MCF7 ceils. We find that the MCAK cooperates with TIP150 to promote microtubule dynamics and modulate the mechanical rigidity of the cells during entosis. Our results show that a dynamic interaction of MCAK-TI P150 orchestrated by Aurora A-mediated phosphorylation governs entosis via regulating microtubule plus-end dynamics and cell rigidity. These data reveal a previously unknown mechanism of Aurora A regulation in the control of microtubule plasticity during ceU-in-ceU pro- cesses.
基金supported by the National Natural Science Foundation of China (Grant No. 51005230)China Postdoctoral Science Foundation(Grant No. 2012M520654)the Education Department of Liaoning Province Science and Technology Research Projects (Grant No. L2012213)
文摘The large-scale assembly and fabrication method for single-walled carbon nanotube(SWCNT) nano devices was implemented.Assembly of SWCNT field effect transistor(FET) was realized by floating potential dielectrophoresis approach.The simulation of floating potential distribution of the chip was performed by comsol multiphysics coupling software.Six hundred devices were assembled on the area of less than one square centimeter.The fabricated devices were characterized by atomic force microscopy and scanning electron microscopy.The experimental results showed that large-scale assembly had been realized,and the success rate of ideal assembly for SWCNT FET had been assessed.
