Ultrastructural changes in the sieve element and dense cells of nectariferous tissue during the development of floral nectary in Arabidopsis thaliana L. were investigated with transmission electron microscopy. Samples...Ultrastructural changes in the sieve element and dense cells of nectariferous tissue during the development of floral nectary in Arabidopsis thaliana L. were investigated with transmission electron microscopy. Samples were prepared with high pressure freezing and freeze substitution techniques. The ultrastructure of dense cell was similar to that of sieve element at its early developmental stage. With the concurrent agglutination of chromatin in the nucleus, the abnormal location of organelles and the high density of cytoplasm, the ultrastructural characteristics in die dense cells of the nectariferous tissue and in the sieve element are matched with those of the programmed cell death in animal and plant reported in recent years. The disorganization of nucleus and most organelles in the differentiation of sieve elements and dense cells is closely associated with the transportation and modification of pre-nectar and the transference of nectar. This suggests that the cytological changes in sieve element and nectariferous tissue are closely associated with the nectary functional activities.展开更多
[Objective] The experiment aimed to study chromatographic fingerprint in volatile components of acacia honey and provide scientific evaluation and effective control on quality of acacia honey.[Method] Using solid-phas...[Objective] The experiment aimed to study chromatographic fingerprint in volatile components of acacia honey and provide scientific evaluation and effective control on quality of acacia honey.[Method] Using solid-phase microextraction method to separate and detect volatile components and construct chromatographic fingerprint.[Result] The honey was preheated for 15 min in water bath at 40 ℃ and solid-phase microextraction 85 μmPA was used to extract in overhead air about 30 min,then put it into the injector and desorpted 3 min,which is in 230 ℃.The Supelco WaxTM10 30 m×0.25 mm×0.25 μm column and gradient heating program was the best method to separate volatile components from honey.83 fingerprint peaks were constructed,among which 17 common fingerprint peaks were comprised of chromatographic fingerprint of volatile components of acacia honey.[Conclusion] The chromatographic fingerprint could provide reference for quality control of acacia honey.展开更多
20 localities were randomly selected in Eastern Black Sea Region of Turkey and samples were collected from around the beehives from April to September. Total of 4,640 dead adult worker bees were examined during the st...20 localities were randomly selected in Eastern Black Sea Region of Turkey and samples were collected from around the beehives from April to September. Total of 4,640 dead adult worker bees were examined during the study. Total infection rate in worker bees was 21.23%. Nosema ceranae was identified in all localities with molecular techniques. Temperature and humidity values were measured from around the beehives during field studies. The infection rate ofN. ceranae increased proportionally with increasing temperature and humidity factors. Humidity was more effective than temperature on the infection rate ofN. ceranae. The seasonal activity ofN. ceranae was studied. The highest infection rates were observed in June and July. N. ceranae infection rate was higher in localities that were in low-altitude than in localities that were in high-altitude.展开更多
Propolis collected by stingless bees from various types of plants has been used as an antimicrobial agent in several previous studies. We assessed the effect of propolis produced by a stingless bee, Trigona apicalis, ...Propolis collected by stingless bees from various types of plants has been used as an antimicrobial agent in several previous studies. We assessed the effect of propolis produced by a stingless bee, Trigona apicalis, on Apis florea experimentally infected with Nosema ceranae, a parasite of honeybees. For parasite inoculation each Nosema free-bee was fed 2μL of 50% (w/v) sucrose solution containing N. ceranae spores at 40,000 spores/bee and 0 as a negative control (CO). Treated bees were provided with 0%, 10%, 20% and 50% propolis (w/v) in water, defined as 0P, 10P, 20P and 50P, respectively. We assessed the effects of propolis 14 days post inoculation. All propolis-treated bees had significantly higher survival than untreated bees. However, survival of Nosema-inoculated bees was lower than that of control bees. Bees treated with the highest propolis concentration (50P) had the highest survival ratio. No control bees became infected over the course of the study. However, N. ceranae infection rates of bees treated with 0P, 10P, 20P and 50P were 75 ± 1.4%, 72 ± 5.6%, 69± 4.2% and 47± 1.4%, respectively. In addition, propolis-treated bees had hypopharyngeal gland protein content that was significantly higher than 0P and CO bees. Overall, propolis treatment significantly reduced N. ceranae infection rate and bee mortality and was associated with increased hypopharyngeal gland protein concentration.展开更多
文摘Ultrastructural changes in the sieve element and dense cells of nectariferous tissue during the development of floral nectary in Arabidopsis thaliana L. were investigated with transmission electron microscopy. Samples were prepared with high pressure freezing and freeze substitution techniques. The ultrastructure of dense cell was similar to that of sieve element at its early developmental stage. With the concurrent agglutination of chromatin in the nucleus, the abnormal location of organelles and the high density of cytoplasm, the ultrastructural characteristics in die dense cells of the nectariferous tissue and in the sieve element are matched with those of the programmed cell death in animal and plant reported in recent years. The disorganization of nucleus and most organelles in the differentiation of sieve elements and dense cells is closely associated with the transportation and modification of pre-nectar and the transference of nectar. This suggests that the cytological changes in sieve element and nectariferous tissue are closely associated with the nectary functional activities.
基金Support by Department of Education Science and Technology Research Projects of Hebei Province(2008310)the National Special Fund for the Commonweal Industry(200810345)~~
文摘[Objective] The experiment aimed to study chromatographic fingerprint in volatile components of acacia honey and provide scientific evaluation and effective control on quality of acacia honey.[Method] Using solid-phase microextraction method to separate and detect volatile components and construct chromatographic fingerprint.[Result] The honey was preheated for 15 min in water bath at 40 ℃ and solid-phase microextraction 85 μmPA was used to extract in overhead air about 30 min,then put it into the injector and desorpted 3 min,which is in 230 ℃.The Supelco WaxTM10 30 m×0.25 mm×0.25 μm column and gradient heating program was the best method to separate volatile components from honey.83 fingerprint peaks were constructed,among which 17 common fingerprint peaks were comprised of chromatographic fingerprint of volatile components of acacia honey.[Conclusion] The chromatographic fingerprint could provide reference for quality control of acacia honey.
文摘20 localities were randomly selected in Eastern Black Sea Region of Turkey and samples were collected from around the beehives from April to September. Total of 4,640 dead adult worker bees were examined during the study. Total infection rate in worker bees was 21.23%. Nosema ceranae was identified in all localities with molecular techniques. Temperature and humidity values were measured from around the beehives during field studies. The infection rate ofN. ceranae increased proportionally with increasing temperature and humidity factors. Humidity was more effective than temperature on the infection rate ofN. ceranae. The seasonal activity ofN. ceranae was studied. The highest infection rates were observed in June and July. N. ceranae infection rate was higher in localities that were in low-altitude than in localities that were in high-altitude.
文摘Propolis collected by stingless bees from various types of plants has been used as an antimicrobial agent in several previous studies. We assessed the effect of propolis produced by a stingless bee, Trigona apicalis, on Apis florea experimentally infected with Nosema ceranae, a parasite of honeybees. For parasite inoculation each Nosema free-bee was fed 2μL of 50% (w/v) sucrose solution containing N. ceranae spores at 40,000 spores/bee and 0 as a negative control (CO). Treated bees were provided with 0%, 10%, 20% and 50% propolis (w/v) in water, defined as 0P, 10P, 20P and 50P, respectively. We assessed the effects of propolis 14 days post inoculation. All propolis-treated bees had significantly higher survival than untreated bees. However, survival of Nosema-inoculated bees was lower than that of control bees. Bees treated with the highest propolis concentration (50P) had the highest survival ratio. No control bees became infected over the course of the study. However, N. ceranae infection rates of bees treated with 0P, 10P, 20P and 50P were 75 ± 1.4%, 72 ± 5.6%, 69± 4.2% and 47± 1.4%, respectively. In addition, propolis-treated bees had hypopharyngeal gland protein content that was significantly higher than 0P and CO bees. Overall, propolis treatment significantly reduced N. ceranae infection rate and bee mortality and was associated with increased hypopharyngeal gland protein concentration.