Micropipette aspiration(MA) is widely applied in cell mechanics, however, at small deformations a common model corresponding to the MA is the half-space model wherein the finite cell size and cell compressibility are ...Micropipette aspiration(MA) is widely applied in cell mechanics, however, at small deformations a common model corresponding to the MA is the half-space model wherein the finite cell size and cell compressibility are neglected. This study extends the half-space model by accounting for the influence of cell geometry and compressibility(sphere model). Using a finite element analysis of cell aspiration into a micropipette, an elastic approximation formula of the aspirated length was derived for the sphere model. The approximation formula includes the geometry parameter of the sphere model(ζ = R/a, R is the radius of the cell, and a is the inner radius of the micropipette) and the Poisson's ratio v of the cell. The results indicate that the parameter and Poisson's ratio v markedly affect the aspirated length, particularly for small and v. When ζ→∞ and v→0.5,the approximation formula tends to the analytical solution for the half-space model. In the incompressible case(v = 0.5), within the general experimental range(ζ varying from 2 to 4), the difference between the analytical solution and the approximate one is significant, and is up to 29% of the approximation solution when ζ= 2. Additionally, parametere was introduced to evaluate the error of elastic moduli between the half-space model and sphere model. Based on the approximation formula, the ζ thresholds, beyond which e becomes larger than 10% and 20%, were derived.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.11032008)the Youth Fund of Taiyuan University of Technology
文摘Micropipette aspiration(MA) is widely applied in cell mechanics, however, at small deformations a common model corresponding to the MA is the half-space model wherein the finite cell size and cell compressibility are neglected. This study extends the half-space model by accounting for the influence of cell geometry and compressibility(sphere model). Using a finite element analysis of cell aspiration into a micropipette, an elastic approximation formula of the aspirated length was derived for the sphere model. The approximation formula includes the geometry parameter of the sphere model(ζ = R/a, R is the radius of the cell, and a is the inner radius of the micropipette) and the Poisson's ratio v of the cell. The results indicate that the parameter and Poisson's ratio v markedly affect the aspirated length, particularly for small and v. When ζ→∞ and v→0.5,the approximation formula tends to the analytical solution for the half-space model. In the incompressible case(v = 0.5), within the general experimental range(ζ varying from 2 to 4), the difference between the analytical solution and the approximate one is significant, and is up to 29% of the approximation solution when ζ= 2. Additionally, parametere was introduced to evaluate the error of elastic moduli between the half-space model and sphere model. Based on the approximation formula, the ζ thresholds, beyond which e becomes larger than 10% and 20%, were derived.
