Esox reieherti Dybowsk genomic microsateUites were developed by using enrichment protocols combined with radioactive hybridization protocol. Four hundred to nine hundred base pair fragments were selected for the whole...Esox reieherti Dybowsk genomic microsateUites were developed by using enrichment protocols combined with radioactive hybridization protocol. Four hundred to nine hundred base pair fragments were selected for the whole genome. DNA PCR amplification after digestion with restriction endonuclease Sau 3A Ⅰ, and (CA)12, (GA)12 probes marked with biotin were used for microsateUite DNA enrichment. The product fragments were connected with carder pGEM-T and transferred into DH5α Escherichia coli competent cells, and radioactive isotope probes marked with γ^-32 p were used for the second hybridization. As a result, a total of 1600 bacteria were obtained in the microsatellite genomic libraries, positive clones accounted for 90.91% before hybridization and 81.25% after hybridization, amounting to 1300. One hundred and ninety-six positive clones were selected for sequencing, and 192 clones included microsateUite sequences. The microsateUite sequences obtained, mono-nucleotide, quad-nucleotide and quint-nucleotide repeat motifs were observed beside double-base-pairs CA/GT, GA/CT. Seventy primers were designed according to the flanking sequences by using software Primer Premier 5.0, and 32 primers were selected to be synthesized. After optimizing PCR reaction conditions, 28 primers were amplified and produced clear purpose bands. The aim of our research was to promote the development and utilization of E. reieherti genomic resource, and lay the foundation for optimizing E. reieherti breeding strain in order to detect the genetic diversity and construct a genetic map.展开更多
Conventional mechanical method and mechanical method combined with vacuum freeze-drying technology were used to make the ultra-fine powders of edible fungus (Auricularia auricular). The content of basic nutrients, ami...Conventional mechanical method and mechanical method combined with vacuum freeze-drying technology were used to make the ultra-fine powders of edible fungus (Auricularia auricular). The content of basic nutrients, amino acid, micro structure and their properties of raw edible fungus and the edible fungus powders obtained with the two methods were analyzed and compared. The granularity size and micro-structure of the pulverized samples were analyzed by SEM and TEM technology. The average granularity size of the edible fungus powder obtained with mechanical method was 1–5 μm, while that obtained with mechanical method combined with vacuum freeze-drying process was 0.5–1 μm. The ultra-fine powders of edible fungus obtained with the two methods had better water recovery capability and quality, and their preserving time was longer than that of raw edible fungus. All the properties of the ultra-fine powders of edible fungus obtained with the vacuum freeze-drying technology were evidently superior to that of the conventional mechanical method. Keywords Auricularia auricula - Edible fungus - Ultra-fine powders - Vacuum freeze-drying CLC number TS205 Document code B Biography: YANG Chun-yu (1975), female, Ph. D. in Engineering Technology Center of Forestry and Wood Workine, Machinery, Northeast Forestry University, Harbin 150040, P. R. China.Responsible editor: Zhu Hong展开更多
In order to improve the anticoagulant function of Auricularia auricula, Auricularia auricula polysaccharide (AAP) was converted into its derivatives by the microbial fermentation method and then polysaccharide deriv...In order to improve the anticoagulant function of Auricularia auricula, Auricularia auricula polysaccharide (AAP) was converted into its derivatives by the microbial fermentation method and then polysaccharide derivatives with stronger anticoagulant activity were prepared. The optimal conditions for fermenting the polysaccharide from A. auricula were examined and the in vitro anticoagulant activities of transformed and untransformed polysaccharides were compared. Response surface tests and an orthogonal experiment indicated that the best conditions for microbial conversion of AAP3 were an AAP3 concentration of 4.0 mg/ mL, a ratio of substrate (AAP3 ) to donor (p-hydroxybenzoic acid) of 40:1, and a pH of 6.0. Bacillus subtilis Bs-07 was inoculated and then placed on a rotary shaker ( 120 r/min), followed by fermentation for 48 h at 35 ℃. The conversion rate was found to be greater than 40%. The result of in vitro anticoagulant activity showed that the transformed polysaccharide improved activated partial thromboplastin time, prothrombin time, and thrombin time values and greatly enhanced anticoagulant activity compared to the untransformed polysaccharide.展开更多
The Aronia melanocarpa El oit was used as test material. The microstruc-ture of normal and vitrified shoots and the characteristics of their stomas on leaf surface were compared by paraffin section and leaf epidermis-...The Aronia melanocarpa El oit was used as test material. The microstruc-ture of normal and vitrified shoots and the characteristics of their stomas on leaf surface were compared by paraffin section and leaf epidermis-tearing method. The results showed the palisade tissue of Aronia melanocarpa El oit consists of 2-3 lay-ers of cells. The stomas on lower epidermis cave in, and are smal and dense. There are abundant vessels and sieve tubes in stems. ln contrast, the main veins of vitrified shoots are unobvious, messy and irregular. The boundary between pal-isade tissue and spongy tissue is not obvious. The stomas open circularly and bigly. The stems are swel ing and thick, but the pith parenchyma cells are broken.展开更多
To prepare potassium titanate catalyst, a novel citrate acid complex-combustion method using CH3COOK and Ti(OC4H9)4 as raw materials was developed. The crystalline phase and surface morphology of K2Ti205 were invest...To prepare potassium titanate catalyst, a novel citrate acid complex-combustion method using CH3COOK and Ti(OC4H9)4 as raw materials was developed. The crystalline phase and surface morphology of K2Ti205 were investigated by X-ray diffraction (XRD), field emission scanning electron microscope (FE-SEM) and transmission electron microscope (TEM). The impact of some factors, such as the type of contact between K2Ti205 and soot, the content of water vapor and SO2 in exhaust, and the repeated use on catalytic activity of K2Ti205 were studied by temperature programmed reaction (TPR). A comparison between the new method and the reported ones on catalytic activity of potassium titanate was investigated. The results showed that K2Ti205 had high catalytic activity and good stability.展开更多
Religious, occasional and annual fire work festivals worldwide are found to introduce high concentrations of pollutant gases and particulate matter into the atmosphere. These in turn alters the air quality of a region...Religious, occasional and annual fire work festivals worldwide are found to introduce high concentrations of pollutant gases and particulate matter into the atmosphere. These in turn alters the air quality of a region in the short and long time scales and affect human health adversely. The effect of fireworks on short-term variation in air quality of an urban location Dibrugarh, set amidst the rural environment of north-east India, was assessed from the ambient concentrations of 03, SO2, NOx, CO and BC (black carbon) during the Diwali festival during Nov. 13-14, 2012. The firework activity in general peaks during evening to midnight hours. Substantial increase in concentrations of O3, SO2, NOx, CO and BC was observed during the peak hours of firework activity (16:00 h to 22:00 h) in the Day 1 and Day 2 of the Diwali festival. However, the increase in concentrations from the background level was found to be highest on the post-Diwali day due to their accumulation in the atmosphere. The firework activity has not affected the regular diurnal pattern of the measured species during this episode.展开更多
A successful design, previously adapted for treatment of complex wastewaters in a microbial fuel cell (MFC), was used to fabricate two MFCs, with a few changes for cost reduction and ease of construction. Performanc...A successful design, previously adapted for treatment of complex wastewaters in a microbial fuel cell (MFC), was used to fabricate two MFCs, with a few changes for cost reduction and ease of construction. Performance and electrochemical characteristics of MFCs were evaluated in different environmental conditions (in complete darkness and presence of light), and different flow patterns of batch and continuous in four hydraulic retention times from 8 to 30 h. Changes in chemical oxygen demand, and nitrate and phosphate concentrations were evaluated. In contrast to the microbial fuel cell operated in darkness (D-MFC) with a stable open circuit voltage of 700 mV, presence of light led to growth of other species, and consecutively low and unsteady open circuit voltage. Although the performance of the MFC subjected to light (L-MFC) was quite low and unsteady in dynamic state (internal resistance = 100 Ω, power density = 5.15 W.m-3). it reached power density of 9.2 W.m-3 which was close to performance of D-MFC (internal resistance = 50 d, power density = 10.3 W.m-3). Evaluated only for D-MFC, the coulombic efficiency observed in batch mode (30%) was quite higher than the maximum acquired in continuous mode (9.6%) even at the highest hydraulic retention time. In this study, changes in phosphate and different types of nitrogen existing in dairy wastewater were investigated for the first time. At hydraulic retention time of 8 h, the orthophosphate concentration in effluent was 84% higher compared to influent. Total nitrogen and total Kjeldahl nitrogen were reduced 70% and 99% respectively at hydraulic retention time of 30 h, while nitrate and nitrite concentrations increased. The microbial electrolysis cell (MEC), revamped from D-MEC, showed the maximum gas production of 0.2 m3 H2·m-3·d-1 at 700 mV applied voltage.展开更多
The effect of surfactant polyoxyethylenesorbitan monolaurate (Tween-20) on the nickel bioleaching from pre-treated chromite overburden (COB), Sukinda with fungal strain Aspergillus niger, was examined in shake fla...The effect of surfactant polyoxyethylenesorbitan monolaurate (Tween-20) on the nickel bioleaching from pre-treated chromite overburden (COB), Sukinda with fungal strain Aspergillus niger, was examined in shake flasks. Along with the nickel recovery from COB by the fungal bioleaching, the effect of surfactant on the growth of the A. niger was also investigated. Results show that the addition of surfactant in low concentration was favorable for the recovery of nickel from pre-treated COB. Normally, the carbon source (sucrose) in the culture medium was utilized by the A. niger for its cellular metabolism and organic metabolites (bio acids) were produced, which were responsible for the bioleaching of minerals. However, the addition of surfactant (Tween-20) accelerated the rate of sucrose consumption by the fungi, and thus enhancing the extraction of nickel from pre-treated COB. During the study, around 39% nickel extraction was achieved in A. niger mediated bioleaching performed at 2% pulp density of pre-treated COB at 30 °C, in the presence of surfactant whereas only 24% nickel was extracted without surfactant.展开更多
文摘Esox reieherti Dybowsk genomic microsateUites were developed by using enrichment protocols combined with radioactive hybridization protocol. Four hundred to nine hundred base pair fragments were selected for the whole genome. DNA PCR amplification after digestion with restriction endonuclease Sau 3A Ⅰ, and (CA)12, (GA)12 probes marked with biotin were used for microsateUite DNA enrichment. The product fragments were connected with carder pGEM-T and transferred into DH5α Escherichia coli competent cells, and radioactive isotope probes marked with γ^-32 p were used for the second hybridization. As a result, a total of 1600 bacteria were obtained in the microsatellite genomic libraries, positive clones accounted for 90.91% before hybridization and 81.25% after hybridization, amounting to 1300. One hundred and ninety-six positive clones were selected for sequencing, and 192 clones included microsateUite sequences. The microsateUite sequences obtained, mono-nucleotide, quad-nucleotide and quint-nucleotide repeat motifs were observed beside double-base-pairs CA/GT, GA/CT. Seventy primers were designed according to the flanking sequences by using software Primer Premier 5.0, and 32 primers were selected to be synthesized. After optimizing PCR reaction conditions, 28 primers were amplified and produced clear purpose bands. The aim of our research was to promote the development and utilization of E. reieherti genomic resource, and lay the foundation for optimizing E. reieherti breeding strain in order to detect the genetic diversity and construct a genetic map.
文摘Conventional mechanical method and mechanical method combined with vacuum freeze-drying technology were used to make the ultra-fine powders of edible fungus (Auricularia auricular). The content of basic nutrients, amino acid, micro structure and their properties of raw edible fungus and the edible fungus powders obtained with the two methods were analyzed and compared. The granularity size and micro-structure of the pulverized samples were analyzed by SEM and TEM technology. The average granularity size of the edible fungus powder obtained with mechanical method was 1–5 μm, while that obtained with mechanical method combined with vacuum freeze-drying process was 0.5–1 μm. The ultra-fine powders of edible fungus obtained with the two methods had better water recovery capability and quality, and their preserving time was longer than that of raw edible fungus. All the properties of the ultra-fine powders of edible fungus obtained with the vacuum freeze-drying technology were evidently superior to that of the conventional mechanical method. Keywords Auricularia auricula - Edible fungus - Ultra-fine powders - Vacuum freeze-drying CLC number TS205 Document code B Biography: YANG Chun-yu (1975), female, Ph. D. in Engineering Technology Center of Forestry and Wood Workine, Machinery, Northeast Forestry University, Harbin 150040, P. R. China.Responsible editor: Zhu Hong
基金Heilongjiang Province Application Technology Research and Development Plan(No.GA13B202)Heilongjiang Postdoctoral Foundation(No.LBH-Z13008)
文摘In order to improve the anticoagulant function of Auricularia auricula, Auricularia auricula polysaccharide (AAP) was converted into its derivatives by the microbial fermentation method and then polysaccharide derivatives with stronger anticoagulant activity were prepared. The optimal conditions for fermenting the polysaccharide from A. auricula were examined and the in vitro anticoagulant activities of transformed and untransformed polysaccharides were compared. Response surface tests and an orthogonal experiment indicated that the best conditions for microbial conversion of AAP3 were an AAP3 concentration of 4.0 mg/ mL, a ratio of substrate (AAP3 ) to donor (p-hydroxybenzoic acid) of 40:1, and a pH of 6.0. Bacillus subtilis Bs-07 was inoculated and then placed on a rotary shaker ( 120 r/min), followed by fermentation for 48 h at 35 ℃. The conversion rate was found to be greater than 40%. The result of in vitro anticoagulant activity showed that the transformed polysaccharide improved activated partial thromboplastin time, prothrombin time, and thrombin time values and greatly enhanced anticoagulant activity compared to the untransformed polysaccharide.
文摘The Aronia melanocarpa El oit was used as test material. The microstruc-ture of normal and vitrified shoots and the characteristics of their stomas on leaf surface were compared by paraffin section and leaf epidermis-tearing method. The results showed the palisade tissue of Aronia melanocarpa El oit consists of 2-3 lay-ers of cells. The stomas on lower epidermis cave in, and are smal and dense. There are abundant vessels and sieve tubes in stems. ln contrast, the main veins of vitrified shoots are unobvious, messy and irregular. The boundary between pal-isade tissue and spongy tissue is not obvious. The stomas open circularly and bigly. The stems are swel ing and thick, but the pith parenchyma cells are broken.
基金the financial supports provided for this research by the Education Department of Liaoning Province of China(No.2009T061)the Ministry of Education of China(No.[2010]1561)
文摘To prepare potassium titanate catalyst, a novel citrate acid complex-combustion method using CH3COOK and Ti(OC4H9)4 as raw materials was developed. The crystalline phase and surface morphology of K2Ti205 were investigated by X-ray diffraction (XRD), field emission scanning electron microscope (FE-SEM) and transmission electron microscope (TEM). The impact of some factors, such as the type of contact between K2Ti205 and soot, the content of water vapor and SO2 in exhaust, and the repeated use on catalytic activity of K2Ti205 were studied by temperature programmed reaction (TPR). A comparison between the new method and the reported ones on catalytic activity of potassium titanate was investigated. The results showed that K2Ti205 had high catalytic activity and good stability.
文摘Religious, occasional and annual fire work festivals worldwide are found to introduce high concentrations of pollutant gases and particulate matter into the atmosphere. These in turn alters the air quality of a region in the short and long time scales and affect human health adversely. The effect of fireworks on short-term variation in air quality of an urban location Dibrugarh, set amidst the rural environment of north-east India, was assessed from the ambient concentrations of 03, SO2, NOx, CO and BC (black carbon) during the Diwali festival during Nov. 13-14, 2012. The firework activity in general peaks during evening to midnight hours. Substantial increase in concentrations of O3, SO2, NOx, CO and BC was observed during the peak hours of firework activity (16:00 h to 22:00 h) in the Day 1 and Day 2 of the Diwali festival. However, the increase in concentrations from the background level was found to be highest on the post-Diwali day due to their accumulation in the atmosphere. The firework activity has not affected the regular diurnal pattern of the measured species during this episode.
基金supported by Sharif University of Technology,Vice President for Research Grant G930111
文摘A successful design, previously adapted for treatment of complex wastewaters in a microbial fuel cell (MFC), was used to fabricate two MFCs, with a few changes for cost reduction and ease of construction. Performance and electrochemical characteristics of MFCs were evaluated in different environmental conditions (in complete darkness and presence of light), and different flow patterns of batch and continuous in four hydraulic retention times from 8 to 30 h. Changes in chemical oxygen demand, and nitrate and phosphate concentrations were evaluated. In contrast to the microbial fuel cell operated in darkness (D-MFC) with a stable open circuit voltage of 700 mV, presence of light led to growth of other species, and consecutively low and unsteady open circuit voltage. Although the performance of the MFC subjected to light (L-MFC) was quite low and unsteady in dynamic state (internal resistance = 100 Ω, power density = 5.15 W.m-3). it reached power density of 9.2 W.m-3 which was close to performance of D-MFC (internal resistance = 50 d, power density = 10.3 W.m-3). Evaluated only for D-MFC, the coulombic efficiency observed in batch mode (30%) was quite higher than the maximum acquired in continuous mode (9.6%) even at the highest hydraulic retention time. In this study, changes in phosphate and different types of nitrogen existing in dairy wastewater were investigated for the first time. At hydraulic retention time of 8 h, the orthophosphate concentration in effluent was 84% higher compared to influent. Total nitrogen and total Kjeldahl nitrogen were reduced 70% and 99% respectively at hydraulic retention time of 30 h, while nitrate and nitrite concentrations increased. The microbial electrolysis cell (MEC), revamped from D-MEC, showed the maximum gas production of 0.2 m3 H2·m-3·d-1 at 700 mV applied voltage.
文摘The effect of surfactant polyoxyethylenesorbitan monolaurate (Tween-20) on the nickel bioleaching from pre-treated chromite overburden (COB), Sukinda with fungal strain Aspergillus niger, was examined in shake flasks. Along with the nickel recovery from COB by the fungal bioleaching, the effect of surfactant on the growth of the A. niger was also investigated. Results show that the addition of surfactant in low concentration was favorable for the recovery of nickel from pre-treated COB. Normally, the carbon source (sucrose) in the culture medium was utilized by the A. niger for its cellular metabolism and organic metabolites (bio acids) were produced, which were responsible for the bioleaching of minerals. However, the addition of surfactant (Tween-20) accelerated the rate of sucrose consumption by the fungi, and thus enhancing the extraction of nickel from pre-treated COB. During the study, around 39% nickel extraction was achieved in A. niger mediated bioleaching performed at 2% pulp density of pre-treated COB at 30 °C, in the presence of surfactant whereas only 24% nickel was extracted without surfactant.