高效液相色谱法同时测定心动颗粒中阿魏酸和欧前胡素的含量

目的建立高效液相色谱法(HPLC)同时测定心动颗粒中阿魏酸和欧前胡素含量。方法色谱柱为Welchrom@C18反相色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈(A)-水(10 mmol/L磷酸)(B),梯度洗脱:0~3.5 min,15%A;3.5~12 min,15%A→25%A;12~21 min,25%A);流速1.0 m L/min;检测波长310 nm;柱温35℃。结果阿魏酸和欧前胡素的质量浓度分别在5.19~51.90μg/m L和5.20~52.05μg/m L浓度范围内呈良好的线性关系(r2>0.9995),平均回收率分别为98.34%和98.06%,RSD分别为1.02%和0.96%(n=6)。结论本测定方法简便、准确,稳定性、重复性好,适用于心动颗粒中阿魏酸和欧前胡素的含量测定。

PARTICULATE SUSPENSION FLOW INDUCED BY SINUSOIDAL PERISTALTIC WAVES THROUGH ECCENTRIC CYLINDERS： THREAD ANNULAR

This paper describes a new model for obtaining analytical solutions of peristaltic flow through eccentric annuli. A mathematical model of peristaltic pumping of a fluid mixture （as blood model） in a circular eccentric cylinders is presented and it is motivated due to the fact that thread injection is a promising method for placing medical implants within the human body with minimum surgical trauma. For the eccentric annuli, the inner cylinder is rigid and moving with a constant velocity V, and the outer one is hollow flexible cylinder that has a sinusoidal wave traveling down its wall. The coupled differential equations for both the fluid and the particle phases have been solved by using two methods and the expressions for the velocity distribution of fluid and particle phase, flow rate, pressure drop, friction forces at the inner and outer cylinders have been derived. The results obtained are discussed in brief. The significance of the particle concentration and the eccentricity parameter as well as the nature of the basic flow has been well explained.