To prepare a hand-made micropore membrane culture plate insert forco-culture. Methods The plate insert was made using plastic centrifuge tube and micropore membrane.After seeding brain capillary endothelial cells (BCE...To prepare a hand-made micropore membrane culture plate insert forco-culture. Methods The plate insert was made using plastic centrifuge tube and micropore membrane.After seeding brain capillary endothelial cells (BCECs) on it (under the effect ofastrocyte-conditioned medium), the plate insert was assessed by analysis of trans-endothelialelectrical resistance (TEER). Results The plate insert has a stability of at least 15 d underculture condition. TEER increased significantly under co-culture condition from (66.1 +- 13.3)Ωcm^2 to (182.2 +- 6.7) Ωcm^2. Conclusion This micropore membrane culture plate insert can beeasily made, on which BCEC culture can be successfully performed. Moreover, it is adjustable andrecyclable. It follows that the plate insert is a useful tool for co-culture and the relatedresearch fields.展开更多
In this paper, the spatial Hill lunar problem is investigated, and the existence of invariant tori of hyperbolic type in a neighborhood of its equilibrium is shown. Moreover,the author checks the non-degenerate condit...In this paper, the spatial Hill lunar problem is investigated, and the existence of invariant tori of hyperbolic type in a neighborhood of its equilibrium is shown. Moreover,the author checks the non-degenerate condition analytically and obtains two-dimensional elliptic invariant tori on its central manifold as well.展开更多
In this paper, we consider a diffusive density-dependent predator-prey model with Crowley-Martin functional responses subject to Neumann boundary condition. We ana- lyze the stability of the positive equilibrium and t...In this paper, we consider a diffusive density-dependent predator-prey model with Crowley-Martin functional responses subject to Neumann boundary condition. We ana- lyze the stability of the positive equilibrium and the existence of spatially homogeneous and inhomogeneous periodic solutions through the distribution of the eigenvalues. The direction and stability of Hopf bifurcation are determined by the normal form theory and the center manifold theory. Finally, numerical simulations are given to verify our theoretical analysis.展开更多
基金NationalMedicine 863Project (No .2 0 0 2AA2Z3 43C)
文摘To prepare a hand-made micropore membrane culture plate insert forco-culture. Methods The plate insert was made using plastic centrifuge tube and micropore membrane.After seeding brain capillary endothelial cells (BCECs) on it (under the effect ofastrocyte-conditioned medium), the plate insert was assessed by analysis of trans-endothelialelectrical resistance (TEER). Results The plate insert has a stability of at least 15 d underculture condition. TEER increased significantly under co-culture condition from (66.1 +- 13.3)Ωcm^2 to (182.2 +- 6.7) Ωcm^2. Conclusion This micropore membrane culture plate insert can beeasily made, on which BCEC culture can be successfully performed. Moreover, it is adjustable andrecyclable. It follows that the plate insert is a useful tool for co-culture and the relatedresearch fields.
文摘In this paper, the spatial Hill lunar problem is investigated, and the existence of invariant tori of hyperbolic type in a neighborhood of its equilibrium is shown. Moreover,the author checks the non-degenerate condition analytically and obtains two-dimensional elliptic invariant tori on its central manifold as well.
文摘In this paper, we consider a diffusive density-dependent predator-prey model with Crowley-Martin functional responses subject to Neumann boundary condition. We ana- lyze the stability of the positive equilibrium and the existence of spatially homogeneous and inhomogeneous periodic solutions through the distribution of the eigenvalues. The direction and stability of Hopf bifurcation are determined by the normal form theory and the center manifold theory. Finally, numerical simulations are given to verify our theoretical analysis.
