诗性美学思维文化视域或意识的共融可以包括认识个体生理感官上的经验体验和思维意识通/同感,亦包括认知主体作为社会、文化、历史人属性的通/同感。”通感”原来是传统文艺理论的美学鉴赏范畴和概念,根据Oxford English Dictionary(上...诗性美学思维文化视域或意识的共融可以包括认识个体生理感官上的经验体验和思维意识通/同感,亦包括认知主体作为社会、文化、历史人属性的通/同感。”通感”原来是传统文艺理论的美学鉴赏范畴和概念,根据Oxford English Dictionary(上海外语教育出版社2004版)synaesthesia的释义：1)pschnology．The production of mental sense-impression relating to one sense by the stimula- tion of another sense,as in colored hearing,also a sensation produced in one part of the body by stimulating of another part,2) The use of metaphors in which terms relating to one kind of sense -impression are used to describe sense-impression of other kinds.3)un...展开更多
Objective To develop a cellular model of preconditioning by a brief period of hypoxia in isolated guinea pig cardiomyocytes and to determine whether or not an ATP sensitive K+ (KATP) channel is involved in ischemic p...Objective To develop a cellular model of preconditioning by a brief period of hypoxia in isolated guinea pig cardiomyocytes and to determine whether or not an ATP sensitive K+ (KATP) channel is involved in ischemic preconditioning. Methods Single myocytes were isolated from the ventricle of adult guinea pigs. The experimental chamber allowed the cells to be exposed to low O2 pressure. During hypoxic preconditioning, the cells were equilibrated with normaxic solution for 10 minutes and then exposed to hypoxia for 5 minutes, followed by 10 minutes of reoxygenation. The cells were then subjected to 20-180 minutes of hypoxia and reoxygenation. Ionic currents were studied with the patch clamp technique in whole-cell and cell-attached configurations. Results A 5-minute hypoxic preconditioning offered a significant protection from cell injury in subsequent hypoxia-reoxygenation. After a latency of more than 15 minutes, hypoxia induced a time-independent outward K+ current which could be blocked by 5?μmol/L glibenclamide. At 10?mV, the current increased from 78±15?pA to 1581±153?pA (P<0.01, n=18). However, the latency to develop KATP channel currents (IKATP) was greatly shortened in preconditioned cells, and the current was increased acceleratively. At 10?mV, the current more than 4?nA was recorded in preconditioning cells. In the single channel recordings, the time interval from the first channel opening to maximum opening was also markedly abbreviated in preconditioned cells. Conclusion Isolated guinea pig cardiomyocytes can be preconditioned with a brief period of hypoxia. This hypoxic preconditioning may modify the KATP channel, and make the channel open more readily during the second hypoxia.展开更多
文摘诗性美学思维文化视域或意识的共融可以包括认识个体生理感官上的经验体验和思维意识通/同感,亦包括认知主体作为社会、文化、历史人属性的通/同感。”通感”原来是传统文艺理论的美学鉴赏范畴和概念,根据Oxford English Dictionary(上海外语教育出版社2004版)synaesthesia的释义：1)pschnology．The production of mental sense-impression relating to one sense by the stimula- tion of another sense,as in colored hearing,also a sensation produced in one part of the body by stimulating of another part,2) The use of metaphors in which terms relating to one kind of sense -impression are used to describe sense-impression of other kinds.3)un...
基金theLeadingSpecialityFundingsof Shanghai! (No 94 Ⅲ 0 0 1)
文摘Objective To develop a cellular model of preconditioning by a brief period of hypoxia in isolated guinea pig cardiomyocytes and to determine whether or not an ATP sensitive K+ (KATP) channel is involved in ischemic preconditioning. Methods Single myocytes were isolated from the ventricle of adult guinea pigs. The experimental chamber allowed the cells to be exposed to low O2 pressure. During hypoxic preconditioning, the cells were equilibrated with normaxic solution for 10 minutes and then exposed to hypoxia for 5 minutes, followed by 10 minutes of reoxygenation. The cells were then subjected to 20-180 minutes of hypoxia and reoxygenation. Ionic currents were studied with the patch clamp technique in whole-cell and cell-attached configurations. Results A 5-minute hypoxic preconditioning offered a significant protection from cell injury in subsequent hypoxia-reoxygenation. After a latency of more than 15 minutes, hypoxia induced a time-independent outward K+ current which could be blocked by 5?μmol/L glibenclamide. At 10?mV, the current increased from 78±15?pA to 1581±153?pA (P<0.01, n=18). However, the latency to develop KATP channel currents (IKATP) was greatly shortened in preconditioned cells, and the current was increased acceleratively. At 10?mV, the current more than 4?nA was recorded in preconditioning cells. In the single channel recordings, the time interval from the first channel opening to maximum opening was also markedly abbreviated in preconditioned cells. Conclusion Isolated guinea pig cardiomyocytes can be preconditioned with a brief period of hypoxia. This hypoxic preconditioning may modify the KATP channel, and make the channel open more readily during the second hypoxia.
