活血祛痰法对自发性高血压大鼠心肌线粒体膜的影响

【目的】探讨活血祛痰法对自发性高血压大鼠 (SHR)心肌线粒体超微结构及其Ca2 +-Mg2 +ATP酶、膜脂质过氧化的作用。【方法】以正常Wistar大鼠为对照组 ,将SHR分为 14周龄组、 2 8周龄组和中药治疗组 ,分别在 14周和 2 8周取材 ,透射电镜分析线粒体膜形态计量 ,并测定线粒体膜Ca2 +-Mg2 +ATP酶活性、膜丙二醛含量。【结果】SHR各组线粒体比表面小于对照组 ,心肌线粒体Ca2 +-Mg2 +ATP酶活性均低于正常对照组 ,而丙二醛含量高于正常对照组 ,且随着SHR病程延长而呈上升趋势 ;中药治疗能降低该趋势。

硒对大鼠心肌线粒体膜保护作用的观察

用低硒和低硒饲料补硒饲养大鼠，１４周后对比观察大鼠心肌线粒体膜脂质过氧化物（ＬＰＯ）含量、谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）和超氧化物歧化物酶（ＳＯＤ）活性。结果显示，补硒组大鼠心肌线粒体ＬＰＯ含量降低，ＧＳＨ－Ｐｘ活性升高，但补硒对线粒体ＳＯＤ活性无明显影响。

对缺氧代谢敏感的鼠心肌线粒体膜^(99)Tc^m-MIBI特异性结合蛋白的研究

目的 探讨99Tcm 甲氧基异丁基异腈 (MIBI)心肌线粒体非膜电位依赖性结合机制。方法 分离SD大鼠心肌线粒体片段和制作心肌超薄切片。用薄层层析法 (TLC)和SDS聚丙酰胺凝胶电泳法 (SDS PAGE)分析99Tcm MIBI特异性结合蛋白。采用有氧 缺氧 再给氧程序分析99Tc MIBI对心肌摄取和清除99Tcm MIBI的影响。结果 在TLC上可见 2个结合99Tcm MIBI计数峰。其中之一Kd 值为 (5 8± 1 7)nmol/L ,Bmax值为 (11 5± 3 1)pmol/mg蛋白质。此结合能被 2 0 μmol/L99Tc MIBI持久替代 ,被 5 0 μmol/L环孢素A短暂替代。在SDS PAGE上展现出 3个99Tcm MIBI标记蛋白质 ,相对分子质量分别为 4 3× 10 4、2 3× 10 4和 1 0× 10 4。未结合的游离99Tcm MIBI仅为 1 6%。 15 μmol/L99Tc MIBI明显加速心肌清除99Tcm MIBI,而不影响其摄取。缺氧代谢也能促进心肌清除99Tcm MIBI,但与99Tc MIBI有协同作用。结论 鼠心肌线粒体膜存在 1种对缺氧代谢敏感的99Tcm MIBI特异性结合蛋白 ,该蛋白质至少包括

克山病心肌线粒体膜氧化损伤的实验研究及其发病学意义

经过多年研究证明克山病是一种以心肌线粒体膜损害为主要特征的地方性心肌病,这次通过用克山病区低硒粮模拟病区居民生活喂养幼豚鼠15周,结果发现血及心、肝等组织,线粒体、微粒体及上清中的硒水平及谷胱甘肽过氧化物酶活性下降,谷胱甘肽转硫酶活性未见升高,甚而降低。脂质过氧化物、氢过氧化物及荧光色脂增加,线粒体膜脂中的心磷脂减少,同时线粒体内膜结合酶的细胞色素氧化酶(CCO)活性下降,即出现了膜的氧化损伤和膜功能障碍。在向提纯的 CCO 中加心磷脂的重组复性试验中实现了 CCO 活性的明显增加,可见线粒体膜结构与功能的异常是克山病患者心肌能量代谢障碍乃至心肌坏死等病理改变的基础。

Effects of seawater immersion on the functions of mitochondria of myocardium and hepatocyte in hemorrhagic shock rats

Objective: To investigate the effects of seawater immersion on the function of myocardium and hepatocyte mitochondria in experimental hemorrhagic shock rats. Methods: Twenty-four male Wistar rats were divided into three groups (n=8 in each group): control group, HSL group (hemorrhagic shock group on land) and HSS group (hemorrhagic shock group in seawater). The hemodynamic parameters, activities of H+-ATPase (adenosinetriphosphatase), succinate dehydrogenase (SDH) and Ca 2+-Mg 2+-ATPase, the calcium contents in myocardium and hepatocyte mitochondria were measured and the changes of proton translocation across the inner mitochondrial membrane were analyzed. Results: The hemodynamic indexes and the activities of H+-ATPase, SDH, Ca 2+-Mg 2+-ATPase in HSS group were significantly lower than those in control group and HSL group (P< 0.05). In HSS group the calcium levels in tissue and mitochondria of myocardium and hepatocyte were elevated significantly compared with control group and HSL group(P< 0.05). There was no significant difference in proton translocation among three groups. Conclusions: This investigation demonstrates that seawater immersion can aggravate the conditions of hemorrhagic shock rats.