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小鼠心室肌细胞分离方法的改进
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作者 徐明 周士胜 +1 位作者 李龙天 迟庆 《中国临床康复》 CSCD 北大核心 2006年第16期112-113,F0003,共3页
目的:探索更简单实用和重复性好的小鼠心肌细胞分离技术。方法:实验于2005-05/09在大连大学医学院医学研究中心完成。选择8~16周龄昆明小鼠150只,雌雄不拘,体质量30~40g,由大连大学医学院动物中心提供。采用原位主动脉插管和动脉夹固... 目的:探索更简单实用和重复性好的小鼠心肌细胞分离技术。方法:实验于2005-05/09在大连大学医学院医学研究中心完成。选择8~16周龄昆明小鼠150只,雌雄不拘,体质量30~40g,由大连大学医学院动物中心提供。采用原位主动脉插管和动脉夹固定法酶解分离小鼠心室肌细胞,并在室温下进行全细胞膜片钳记录。结果:可将主动脉插管的时间控制在1.5min内,所获心室肌细胞横纹清晰,一次性复钙可获得50%~60%的耐钙细胞,分离所得细胞可在全细胞膜片钳方式下记录到一过性外向钾电流(Ito)。结论:原位主动脉插管和动脉夹固定酶解分离心室肌细胞的方法简便易行,重复性好,克服了原有方法体外心脏插管时间长,每次插管时间差异较大,分离效果不稳定的弊端。 展开更多
关键词 心肌细胞/细胞学 细胞分离 膜片钳术
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Role of cytoskeleton in the mechanisms of stretch-induced cardiomyocytical hypertrophy in vitro
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作者 冯兵 覃军 +1 位作者 何作云 王德文 《Journal of Medical Colleges of PLA(China)》 CAS 2001年第1期1-3,共3页
Objective: To study in vitro the role of cytoskeleton in the mechanisms of stretch-induced cardiomyocyte hypertrophy. Methods: After cultured on a deformable membrane, the myocardial cells were incorporated with 3H-le... Objective: To study in vitro the role of cytoskeleton in the mechanisms of stretch-induced cardiomyocyte hypertrophy. Methods: After cultured on a deformable membrane, the myocardial cells were incorporated with 3H-leucine (3H-leu) to determine the hypertrophic rate. The contents of angiotensin II and endothelin in the supernatant of the culture medium were measured with radioimmunoassay. Results: Colchicine at 4 μmol/L partially inhibited 3H-leu incorporation rate of the stretch-induced cardiomyocytes but cytochalasin B showed no such effect. The radioactivity of 3H-leu incorporation in the supernatant of the culture medium was significantly lower in the cardiomyocyte culture treated with colchicines (μmol/L) or cytochalasin (0.4μmol/L) than in simple myocardial cell culture. In addition, the 2 agents markedly inhibited the myocardial cells from secreting angiotensin II and endothin. Conclusion: The cytoskeleton plays a role in the stretch-induced mycardiocyte hypertrophy by mediating the secretion of the cell growth factors by the cells themselves. 展开更多
关键词 CYTOSKELETON STRETCHING myocardial cells HYPERTROPHY AUTOCRINE
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