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试论藏医三因学说——与哈尔滨德格藏医医院心隆病治疗的临床疗效观察
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作者 四朗青佩 《中外医疗》 2018年第8期127-128,134,共3页
目的采用藏医三因学说对心隆病患者治疗的临床疗效观察。方法方便选取2014年1月—2016年12月该院收治的心隆病患者60例为研究对象,并采用随机数字表法分为对照组和治疗组,每组30例,其中对照组采用西医治疗,辅以藏药七十味珊瑚和二十五... 目的采用藏医三因学说对心隆病患者治疗的临床疗效观察。方法方便选取2014年1月—2016年12月该院收治的心隆病患者60例为研究对象,并采用随机数字表法分为对照组和治疗组,每组30例,其中对照组采用西医治疗,辅以藏药七十味珊瑚和二十五味珍珠丸,治疗组采用藏药进行治疗,比较两组的临床有效率。结果治疗组的临床有效率96.67%要明显优于对照组80.00%,差异有统计学意义(χ~2=4.043,P<0.05)。结论在藏医三因学说的基础上运用藏药进行治疗,多数患者症状基本消失,病情得到很好的控制,心电检查显示正常疗效显著,值得广泛推广。 展开更多
关键词 三因学说 心隆病 赤巴 培根
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Detection of Bluetongue Virus Group-specific Antigen Using Monoclonal Antibody Based Sandwich ELISA 被引量:4
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作者 Pradeep Narayan Gandhale Veerakyathappa Bhanuprakash +3 位作者 Vinayagamurthy Balamurugan Madhusudhan Hosamani Gnanavel Venkatesan Raj Kumar Singh 《Virologica Sinica》 SCIE CAS CSCD 2010年第6期390-400,共11页
A monoclonal antibody (MAb) specific for the bluetongue virus (BTV) group specific antigen (VP7) was characterized for its reactivity with purified virus and recombinant BTV VP7 (rVP7) protein and its suitability for ... A monoclonal antibody (MAb) specific for the bluetongue virus (BTV) group specific antigen (VP7) was characterized for its reactivity with purified virus and recombinant BTV VP7 (rVP7) protein and its suitability for use in the sandwich ELISA.The MAb,designated as 5B5 was specific to VP7 and belongs to IgG2a subclass and was selected for the development of the sELISA in this study.The MAb had a titer of 1:25 with BTV and 1:2 with the rVP7 protein.The sELISA is based on capturing of BTV antigen with VP7 specific MAb followed by detection using BTV polyclonal antiserum raised in rabbits.The assay was evaluated with six cell culture adapted serotypes of BTV that have been isolated from India,1,2,15,17,18 and 23.The assay could detect BTV antigen as early as day 8 in blood.It was also successfully applied for the detection of BTV group specific antigen in clinical samples of blood,washed RBCs,buffy coat and plasma.A total of 102 field samples from animals,suspected of being infected with BTV,were tested and 29.42% were positive.The blood samples were also amplified in cell culture which improved the sensitivity of the assay.Results confirmed that the sELISA is rapid and specific. 展开更多
关键词 BLUETONGUE DIAGNOSIS ELISA Monoclonal antibody Sandwich ELISA
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