Amphioctopus fangsiao is one of the most economically important species and has been considered to be a candidate for aquaculture. In order to facilitate its fine-scale genetic analyses, we constructed a normalized fu...Amphioctopus fangsiao is one of the most economically important species and has been considered to be a candidate for aquaculture. In order to facilitate its fine-scale genetic analyses, we constructed a normalized full-length library successfully and developed a set of microsatellite markers in this study. The normalized full-length library had a storage capacity of 6.9×105 independent clones. The recombination efficiency was 95% and the average size of inserted fragments was longer than 1000 bp. A total of 3440 high quality ESTs were obtained, which were assembled into 1803 unigenes. Of these unigenes, 450(25%) were assigned into 33 Gene Ontology terms, 576(31.9%) into 153 Kyoto Encyclopedia of Genes and Genomes pathways, and 275(15.3%) into 22 Clusters of Orthologous Groups. Seventy-six polymorphic microsatellite markers were identified. The number of alleles per locus ranged from 4 to 17, and the observed and expected heterozygosities varied between 0.167 and 0.967 and between 0.326 and 0.944, respectively. Twelve loci were significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction and no linkage disequilibrium was found between different loci. This study provided not only a useful resource for the isolation of the functional genes, but also a set of informative microsatellites for the assessment of population structure and conservation genetics of A. fangsiao.展开更多
Objective： To investigate the effects of moxibustion on the serum metabolism in healthy human body based on the 1H nuclear magnetic resonance （1H NMR） metabolomics technology, and to find the differences in metabol...Objective： To investigate the effects of moxibustion on the serum metabolism in healthy human body based on the 1H nuclear magnetic resonance （1H NMR） metabolomics technology, and to find the differences in metabolites, as well as to elucidate the effects of moxibustion on healthy human body from the viewpoint of global metabolism. Methods： Sixty subjects of healthy young men from the enrolled students were randomly divided into a moxibustion group and a control group using random number table, with 30 cases in each group. Subjects in the moxibustion group accepted mild moxibustion on the right Zusanli （ST 36）, once a day, 15 min for each time, and continuous treatment for 10 d; those in the control group did not receive any intervention. There were 28 cases in the moxibustion group and 23 cases in the control group after interventions. On the 2st day, 5th day and lOth day of the intervention, serum samples were collected from subjects of the two groups, and metabolic spectra were obtained by the 1H NMR technology. Results： Before and after the intervention, serum 1H NMR of the moxibustion group was significantly different, while the difference was insignificant in the control group. Metabolite changes in the moxibustion group were mainly in low density lipoprotein （LDL）/very low density lipoprotein （VLDL）, valine, isoleucine, leucine, lactic acid, glutamine, citric acid, polyunsaturated fatty acids, creatine, glycine, glycerol, glucose, tyrosine, histidine, formic acid, alanine, lysine, acetic acid, and glutamic acid. Conclusion： Moxibustion can cause changes of serum metabolic patterns in healthy human by influencing the concentrations of branched-chain amino acids, polyunsaturated fatty acids, and other metabolites to strengthen body＇s metabolisms of amino acids and fatty acid.展开更多
Eospirifer,the oldest known genus of the spiriferide group(Brachiopoda),first appeared on the Zhe-Gan Platform of South China paleoplate during late Katian(Late Ordovician) represented by E.praecursor.It survived the ...Eospirifer,the oldest known genus of the spiriferide group(Brachiopoda),first appeared on the Zhe-Gan Platform of South China paleoplate during late Katian(Late Ordovician) represented by E.praecursor.It survived the end-Ordovician mass extinction,and reappeared on the Upper Yangtze Platform of South China paleoplate by the end of Ordovician near the upper part of the Normalograptus persculptus Biozone.Starting from the beginning of Silurian,Eospirifer experienced some morphological innovations and expanded its geographical distribution substantially.It reached its species diversity acme and the widest geographic distribution in Wenlock(middle Silurian),with the diversity hotspots in Laurentia,Avalonia,and Baltica.Various shell size frequency curves of E.praecursor under different paleogeographic settings suggest that this pioneer species of Eospirifer,with several macroevolutionary novelties,adopted a range of life strategies to adapt to the changing environments during early spiriferide evolution.There are also some morphological macroevolutionary trends during the evolutionary history of Eospirifer from Late Ordovician to the end of Silurian,such as the ever enlarging shell sizes and the width/length ratios from late Katian to Wenlock,but decreasing apparently of both parameters from Wenlock to Pridoli.展开更多
基金the National Natural Science Foundation of China (Nos. 31302215, 31272643)the Shandong Provincial Natural Science Foundation (Nos. BS2014NY010, ZR2013CQ030)the Shandong Provincial Primary Research and Development Projects (No. 2015GNC110017)
文摘Amphioctopus fangsiao is one of the most economically important species and has been considered to be a candidate for aquaculture. In order to facilitate its fine-scale genetic analyses, we constructed a normalized full-length library successfully and developed a set of microsatellite markers in this study. The normalized full-length library had a storage capacity of 6.9×105 independent clones. The recombination efficiency was 95% and the average size of inserted fragments was longer than 1000 bp. A total of 3440 high quality ESTs were obtained, which were assembled into 1803 unigenes. Of these unigenes, 450(25%) were assigned into 33 Gene Ontology terms, 576(31.9%) into 153 Kyoto Encyclopedia of Genes and Genomes pathways, and 275(15.3%) into 22 Clusters of Orthologous Groups. Seventy-six polymorphic microsatellite markers were identified. The number of alleles per locus ranged from 4 to 17, and the observed and expected heterozygosities varied between 0.167 and 0.967 and between 0.326 and 0.944, respectively. Twelve loci were significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction and no linkage disequilibrium was found between different loci. This study provided not only a useful resource for the isolation of the functional genes, but also a set of informative microsatellites for the assessment of population structure and conservation genetics of A. fangsiao.
基金supported by National Basic Research Program of China 973 Program(No.2015CB554502)National Natural Science Foundation of China(No.81202770+4 种基金No.81574082)Special Research Fund for the Doctoral Program of Higher Education of China for New Teachers(No.20124323120002)Foundation for the Author of Excellent Doctoral Dissertation of Hunan Province(No.YB2013B037)Fund Project of Hunan Province Education Office(No.14B129)2015 Graduate Student Research Innovation Project of Hunan Province(No.CX2015B320)~~
文摘Objective： To investigate the effects of moxibustion on the serum metabolism in healthy human body based on the 1H nuclear magnetic resonance （1H NMR） metabolomics technology, and to find the differences in metabolites, as well as to elucidate the effects of moxibustion on healthy human body from the viewpoint of global metabolism. Methods： Sixty subjects of healthy young men from the enrolled students were randomly divided into a moxibustion group and a control group using random number table, with 30 cases in each group. Subjects in the moxibustion group accepted mild moxibustion on the right Zusanli （ST 36）, once a day, 15 min for each time, and continuous treatment for 10 d; those in the control group did not receive any intervention. There were 28 cases in the moxibustion group and 23 cases in the control group after interventions. On the 2st day, 5th day and lOth day of the intervention, serum samples were collected from subjects of the two groups, and metabolic spectra were obtained by the 1H NMR technology. Results： Before and after the intervention, serum 1H NMR of the moxibustion group was significantly different, while the difference was insignificant in the control group. Metabolite changes in the moxibustion group were mainly in low density lipoprotein （LDL）/very low density lipoprotein （VLDL）, valine, isoleucine, leucine, lactic acid, glutamine, citric acid, polyunsaturated fatty acids, creatine, glycine, glycerol, glucose, tyrosine, histidine, formic acid, alanine, lysine, acetic acid, and glutamic acid. Conclusion： Moxibustion can cause changes of serum metabolic patterns in healthy human by influencing the concentrations of branched-chain amino acids, polyunsaturated fatty acids, and other metabolites to strengthen body＇s metabolisms of amino acids and fatty acid.
基金supported by Chinese Academy of Sciences (Grant No. KZCX2-YW-Q05-01)National Natural Science Foundation of China(Grant No. 40825006)the CAS/SAFEA International Partnership Program for Creative Research Teams and the State Key Laboratory of Paleobiology and Stratigraphy
文摘Eospirifer,the oldest known genus of the spiriferide group(Brachiopoda),first appeared on the Zhe-Gan Platform of South China paleoplate during late Katian(Late Ordovician) represented by E.praecursor.It survived the end-Ordovician mass extinction,and reappeared on the Upper Yangtze Platform of South China paleoplate by the end of Ordovician near the upper part of the Normalograptus persculptus Biozone.Starting from the beginning of Silurian,Eospirifer experienced some morphological innovations and expanded its geographical distribution substantially.It reached its species diversity acme and the widest geographic distribution in Wenlock(middle Silurian),with the diversity hotspots in Laurentia,Avalonia,and Baltica.Various shell size frequency curves of E.praecursor under different paleogeographic settings suggest that this pioneer species of Eospirifer,with several macroevolutionary novelties,adopted a range of life strategies to adapt to the changing environments during early spiriferide evolution.There are also some morphological macroevolutionary trends during the evolutionary history of Eospirifer from Late Ordovician to the end of Silurian,such as the ever enlarging shell sizes and the width/length ratios from late Katian to Wenlock,but decreasing apparently of both parameters from Wenlock to Pridoli.
