Ubiquitous on earth,bacteriophages are the most abundant entities in every ecosystem,but human knowledge about them is still limited compared with that about other forms of organisms.To enrich human knowledge and prom...Ubiquitous on earth,bacteriophages are the most abundant entities in every ecosystem,but human knowledge about them is still limited compared with that about other forms of organisms.To enrich human knowledge and promote the utilization of bacteriophages,it is necessary to isolate and characterize as many as possible different bacteriophages.Here we describe the isolation of a T4-like bacteriophage IME08 and a rapid method for its genetic characterization.With this method we easily cloned a few random fragments of the bacteriophage genome.Sequence analysis of these random clones showed that bacteriophage IME08 shared the highest sequence similarity with T4-like Enterobacteria phage T4（94%identity）,JS98（95% identity）,JS10（95%identity） and RB14（94%identity） respectively,which suggested that IME08 belonged to T4-like bacteriophage genus.展开更多
The paper proposes a robust digital audio watermarking scheme using blind source separation(BSS) based on the global optimization of independency metric(IM),which is formulated as a generalized eigenvalue(GE) problem....The paper proposes a robust digital audio watermarking scheme using blind source separation(BSS) based on the global optimization of independency metric(IM),which is formulated as a generalized eigenvalue(GE) problem.Compared with traditional information-theoretical approaches used in digital audio watermarking,such as fast independent component analysis(FastICA),the proposed scheme has lower complexity without timeconsuming iteration steps used in FastICA.To make full use of the multiresolution characteristic of discrete wavelet transform(DWT) and the energy compression characteristic of discrete cosine transform(DCT),the watermark is embedded in the middle DWT-DCT coefficients and the independent component analysis(ICA) approach based on IM is used in the detecting scheme.Simulation results based on Stirmark for Audio v02 show that the proposed scheme has strong robustness as well as the imperceptibility and security.展开更多
文摘Ubiquitous on earth,bacteriophages are the most abundant entities in every ecosystem,but human knowledge about them is still limited compared with that about other forms of organisms.To enrich human knowledge and promote the utilization of bacteriophages,it is necessary to isolate and characterize as many as possible different bacteriophages.Here we describe the isolation of a T4-like bacteriophage IME08 and a rapid method for its genetic characterization.With this method we easily cloned a few random fragments of the bacteriophage genome.Sequence analysis of these random clones showed that bacteriophage IME08 shared the highest sequence similarity with T4-like Enterobacteria phage T4（94%identity）,JS98（95% identity）,JS10（95%identity） and RB14（94%identity） respectively,which suggested that IME08 belonged to T4-like bacteriophage genus.
基金the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry of Chinathe National Natural Science Foundation of China (No. 60802058)
文摘The paper proposes a robust digital audio watermarking scheme using blind source separation(BSS) based on the global optimization of independency metric(IM),which is formulated as a generalized eigenvalue(GE) problem.Compared with traditional information-theoretical approaches used in digital audio watermarking,such as fast independent component analysis(FastICA),the proposed scheme has lower complexity without timeconsuming iteration steps used in FastICA.To make full use of the multiresolution characteristic of discrete wavelet transform(DWT) and the energy compression characteristic of discrete cosine transform(DCT),the watermark is embedded in the middle DWT-DCT coefficients and the independent component analysis(ICA) approach based on IM is used in the detecting scheme.Simulation results based on Stirmark for Audio v02 show that the proposed scheme has strong robustness as well as the imperceptibility and security.
