建立快速协同浊点萃取-荧光分光光度法测定食品塑料包装材料中浸出苯酚的方法。以直链型的非离子表面活性剂聚乙二醇6000为萃取剂,正辛醇作浊点的诱导协同剂,与无水硫酸钠共同作用将聚乙二醇6000浊点温度降至室温(约20℃),整个实验过程...建立快速协同浊点萃取-荧光分光光度法测定食品塑料包装材料中浸出苯酚的方法。以直链型的非离子表面活性剂聚乙二醇6000为萃取剂,正辛醇作浊点的诱导协同剂,与无水硫酸钠共同作用将聚乙二醇6000浊点温度降至室温(约20℃),整个实验过程仅需3 min,并对影响苯酚萃取率的因素(聚乙二醇6000、无水硫酸钠和正辛醇的用量、溶液pH)进行考察。结果表明,溶液pH6.0、0.2 g/m L聚乙二醇6000 8.0 m L、无水硫酸钠3.0 g及正辛醇5.0 m L的萃取效果最佳。苯酚在0.02~1.60μg/m L范围内线性良好,R2为0.9998,检出限为0.0064μg/m L,相对标准偏差为4.49%(n=8)。对于50 m L的样品溶液,苯酚萃取率可达89.8%,加标回收率为90.5%~109.5%。5种食品塑料包装材料中,只有样品2检出苯酚0.0310μg/m L,但未超标,其它4种塑料包装材料均未检出。该方法简便、快速、省时、灵敏度高,适用于食品塑料包装材料中浸出苯酚的测定。展开更多
Coagulation test, in principle, is an immunodiagnostics technique, in which immunoglobuline G of antibody is bound to protein A from Staphylococcus aureus. The aim of study is to develop a rapid test kit for detecting...Coagulation test, in principle, is an immunodiagnostics technique, in which immunoglobuline G of antibody is bound to protein A from Staphylococcus aureus. The aim of study is to develop a rapid test kit for detecting iridovirus infection in fish. Method was summarized as follows： （1） vaccine of iridovirus was injected to rabbit four times with a dosage as 0.5 mL, 1 mL, 2mL, 3 mL each week. Serum was collected at the fifth week as a coagglutination test kit; （2） through the positif polymerase chain reaction （PCR） test, the kidney and spleen sample infected with iridovirus are homogenized by using the phosphate buffered saline （PBS） solution of pH 7.2 with ratio 1：2 （WN）; （3） the supernatant material is collected after centrifugation at 8,000 rpm for 15 min; （4） filtrate/supernatant from sample was dropped on a slide an added with coagglutination test kit with the same volume （l：l）; （5） the agglutination observation is done after the 30, 60 and 90 min incubate at room temperature. The coagglutination test gave positive result in 25% of the test samples.展开更多
文摘建立快速协同浊点萃取-荧光分光光度法测定食品塑料包装材料中浸出苯酚的方法。以直链型的非离子表面活性剂聚乙二醇6000为萃取剂,正辛醇作浊点的诱导协同剂,与无水硫酸钠共同作用将聚乙二醇6000浊点温度降至室温(约20℃),整个实验过程仅需3 min,并对影响苯酚萃取率的因素(聚乙二醇6000、无水硫酸钠和正辛醇的用量、溶液pH)进行考察。结果表明,溶液pH6.0、0.2 g/m L聚乙二醇6000 8.0 m L、无水硫酸钠3.0 g及正辛醇5.0 m L的萃取效果最佳。苯酚在0.02~1.60μg/m L范围内线性良好,R2为0.9998,检出限为0.0064μg/m L,相对标准偏差为4.49%(n=8)。对于50 m L的样品溶液,苯酚萃取率可达89.8%,加标回收率为90.5%~109.5%。5种食品塑料包装材料中,只有样品2检出苯酚0.0310μg/m L,但未超标,其它4种塑料包装材料均未检出。该方法简便、快速、省时、灵敏度高,适用于食品塑料包装材料中浸出苯酚的测定。
文摘Coagulation test, in principle, is an immunodiagnostics technique, in which immunoglobuline G of antibody is bound to protein A from Staphylococcus aureus. The aim of study is to develop a rapid test kit for detecting iridovirus infection in fish. Method was summarized as follows： （1） vaccine of iridovirus was injected to rabbit four times with a dosage as 0.5 mL, 1 mL, 2mL, 3 mL each week. Serum was collected at the fifth week as a coagglutination test kit; （2） through the positif polymerase chain reaction （PCR） test, the kidney and spleen sample infected with iridovirus are homogenized by using the phosphate buffered saline （PBS） solution of pH 7.2 with ratio 1：2 （WN）; （3） the supernatant material is collected after centrifugation at 8,000 rpm for 15 min; （4） filtrate/supernatant from sample was dropped on a slide an added with coagglutination test kit with the same volume （l：l）; （5） the agglutination observation is done after the 30, 60 and 90 min incubate at room temperature. The coagglutination test gave positive result in 25% of the test samples.
