A new,simple,rapid microbial assay was put forward for the detection of chloramphenicol(CAP)residues in Turbot muscles using luminescent bacteria DB(Photobacterium phosphoreum)as test organ-ism.By the luminescence inh...A new,simple,rapid microbial assay was put forward for the detection of chloramphenicol(CAP)residues in Turbot muscles using luminescent bacteria DB(Photobacterium phosphoreum)as test organ-ism.By the luminescence inhibition of DB,the concentrations of CAP were determined.CAP spiked inTurbot muscle tissues ranging from 0.5 to 100μg/kg was extracted using ethyl acetate and hexane.Themethod had a good recovery of 93.69% with a minimum detection limit of 0.65μg/kg.It can be per-formed within 30min following simple extraction.The method therefore proved to be advantageous overchromatographic procedures as it was inexpensive,quite sensitive and can be adopted for rapid screeningof CAP in Turbot tissues.展开更多
基金Supported by the National Natural Science Foundation of China ( No. 30500384)the High Technology Research and Development Programme of China (No. 2006AA09Z431)
文摘A new,simple,rapid microbial assay was put forward for the detection of chloramphenicol(CAP)residues in Turbot muscles using luminescent bacteria DB(Photobacterium phosphoreum)as test organ-ism.By the luminescence inhibition of DB,the concentrations of CAP were determined.CAP spiked inTurbot muscle tissues ranging from 0.5 to 100μg/kg was extracted using ethyl acetate and hexane.Themethod had a good recovery of 93.69% with a minimum detection limit of 0.65μg/kg.It can be per-formed within 30min following simple extraction.The method therefore proved to be advantageous overchromatographic procedures as it was inexpensive,quite sensitive and can be adopted for rapid screeningof CAP in Turbot tissues.
