To establish a rapid quantification method for heparinase I during its production in recombinant Escherichia coli, a translational fusion vector was constructed by fusing the N terminus of heparinase I to the C termin...To establish a rapid quantification method for heparinase I during its production in recombinant Escherichia coli, a translational fusion vector was constructed by fusing the N terminus of heparinase I to the C terminus of a green fluorescent protein mutant (GFPmutl). As a result, not only was the functional recombinant expression of heparinase I in E. coli accomplished, but also a linear correlation was obtained between the GFP fluorescence intensity and heparinase I activity, allowing enzyme activity to be quantified rapidly during the fermentation.展开更多
Rapidly solidified Sn-9Zn-0.1Pr(/Nd) alloy foils were prepared by melt-spinning method. Through comparison, the effects of rapid solidification process and 0.1%Pr/Nd(mass fraction) addition on the microstructure, ther...Rapidly solidified Sn-9Zn-0.1Pr(/Nd) alloy foils were prepared by melt-spinning method. Through comparison, the effects of rapid solidification process and 0.1%Pr/Nd(mass fraction) addition on the microstructure, thermodynamic characteristic of Sn-9Zn solder alloy were analyzed. The tensile-shear tests were used to evaluate the mechanical properties of solder/Cu joints. The results show that the rapid solidification process can greatly refine the microstructure of Sn-9Zn-0.1Pr(/Nd) alloys. After rapid solidification, the effects of Pr/Nd addition on microstructure are depressed. The pasty range of the rapidly solidified Sn-Zn-RE solders is also reduced significantly. The mechanical properties of solder/Cu joints are obviously improved using the rapidly solidified Sn-9Zn-0.1Pr(/Nd) solder alloy, which results in the formation of uniform interface. The promotion effect of Nd addition in Sn-9Zn alloy on the interfacial reaction of solder/Cu joint is more remarkable than that of Pr.展开更多
With the fast development of highspeed railways,a call for fulfilling the notion of communication at "anytime,anywhere" for high-speed train passengers in the Train Operating Control System is on the way.In ...With the fast development of highspeed railways,a call for fulfilling the notion of communication at "anytime,anywhere" for high-speed train passengers in the Train Operating Control System is on the way.In order to make a realization of that,new railway wireless communication networks are needed.The most promising one is the Long Term Evolution for Railway which will provide broadband access,fast handover,and reliable communication for high mobility users.However,with the increase of speed,the system is subjected to high bit error rate,Doppler frequency shift and handover failure just like other system does.This paper is trying to solve these problems by employing MIMO technique.Specifically,the goal is to provide higher data rate,higher reliability,less delay,and other relative quality of services for passengers.MIMO performance analysis,resource allocation,and access control for handover and various services in a two-hop model are proposed in this paper.Analytical results and simulation results show that the proposed model and schemes perform well in improving the system performances.展开更多
Ubiquitous on earth,bacteriophages are the most abundant entities in every ecosystem,but human knowledge about them is still limited compared with that about other forms of organisms.To enrich human knowledge and prom...Ubiquitous on earth,bacteriophages are the most abundant entities in every ecosystem,but human knowledge about them is still limited compared with that about other forms of organisms.To enrich human knowledge and promote the utilization of bacteriophages,it is necessary to isolate and characterize as many as possible different bacteriophages.Here we describe the isolation of a T4-like bacteriophage IME08 and a rapid method for its genetic characterization.With this method we easily cloned a few random fragments of the bacteriophage genome.Sequence analysis of these random clones showed that bacteriophage IME08 shared the highest sequence similarity with T4-like Enterobacteria phage T4(94%identity),JS98(95% identity),JS10(95%identity) and RB14(94%identity) respectively,which suggested that IME08 belonged to T4-like bacteriophage genus.展开更多
On basis of two definitions that 1. an induced subgraph by a vertex vi E G and its neighbors in G is defined a vertex adjacent closed subgraph denoted by Qi (=G[V(Nvi)]), with the vertex vi called the hub; 2. A r...On basis of two definitions that 1. an induced subgraph by a vertex vi E G and its neighbors in G is defined a vertex adjacent closed subgraph denoted by Qi (=G[V(Nvi)]), with the vertex vi called the hub; 2. A r(k,1)-1 vertices connected graph is called a (k,l)-Ramsey graph denoted by RG(k,l),if and only if 1. RG(k,l) contains only cliques of degree k-1, and its complement contains only cliques of degree l-l; 2. the intersect Qi∩Qj of any two nonadjacent vertices vi and vj of RG(k,1) contains Kk.2, and the intersect Qi∩Qj of any two nonadjacent vertices vi and vj of its complement RG(l,k) contains KI.2. Two theorems that theoreml : the biggest clique in G is contained in some Qi of G, and theorem2: r(k,l)= [ V(RG(k,I)) I +1 are put forward and proved in this paper. With those definitions and theorems as well as analysis of property of chords a method for quick inspection and building RG(k,I) is proposed. Accordingly, RG(10,3) and its complement are built, which are respectively the strongly 29-regular graph and the strongly 10-regular graph on orders 40. We have tested RG(10,3) and its complement RG(3,10),and gotten r(3,10)=41.展开更多
Two definitions are given that Definitionl: an induced subgraph by a vertex vie G and its neighbors in G is defined as a vertex adjacent closed subgraph, and denoted by Qi (=G[V(Nvi)]), with the vertex vi called ...Two definitions are given that Definitionl: an induced subgraph by a vertex vie G and its neighbors in G is defined as a vertex adjacent closed subgraph, and denoted by Qi (=G[V(Nvi)]), with the vertex vi called the hub; and Definition2: A r(k,I)-I vertices graph is called the (k,l)-Ramsey graph, denoted by RG(k,1), if RG(k,1) only contains cliques Kk.1 and the intersect QiNQj of any two nonadjacent vertices vi and vj of RG(k,I) contains only Kk-2. Meanwhile, the RG(k,l)'s complement RG(I,k) contains only cliques Kl.l, and the intersect QiNQj of any two nonadjacent vertices vi and vj of RG(I,k) contains only Ki.2. On the basis of those definitions, two theorems are put forward and proved in this paper. They are Theoreml: the biggest clique in G is contained in some Qi of G, and Theorem 2: r(k,1) = [V(RG(k,I))I + 1. With those definitions and theorems as well as analysis of chord property, a method for quick inspection and building RG(k,1) is proposed. Accordingly, RG(4,6) is built, it is a strongly 14-regular graph on order 35. We have tested RG(4,6) and its complement, as a result, they meet the defintion2, so we proclaim that r(4,6)=36.展开更多
The paper proposes a robust digital audio watermarking scheme using blind source separation(BSS) based on the global optimization of independency metric(IM),which is formulated as a generalized eigenvalue(GE) problem....The paper proposes a robust digital audio watermarking scheme using blind source separation(BSS) based on the global optimization of independency metric(IM),which is formulated as a generalized eigenvalue(GE) problem.Compared with traditional information-theoretical approaches used in digital audio watermarking,such as fast independent component analysis(FastICA),the proposed scheme has lower complexity without timeconsuming iteration steps used in FastICA.To make full use of the multiresolution characteristic of discrete wavelet transform(DWT) and the energy compression characteristic of discrete cosine transform(DCT),the watermark is embedded in the middle DWT-DCT coefficients and the independent component analysis(ICA) approach based on IM is used in the detecting scheme.Simulation results based on Stirmark for Audio v02 show that the proposed scheme has strong robustness as well as the imperceptibility and security.展开更多
基金Supported by the National Natural Science Foundation of China (No.20336010 and No.20176025).
文摘To establish a rapid quantification method for heparinase I during its production in recombinant Escherichia coli, a translational fusion vector was constructed by fusing the N terminus of heparinase I to the C terminus of a green fluorescent protein mutant (GFPmutl). As a result, not only was the functional recombinant expression of heparinase I in E. coli accomplished, but also a linear correlation was obtained between the GFP fluorescence intensity and heparinase I activity, allowing enzyme activity to be quantified rapidly during the fermentation.
基金Project(50675234)supported by the National Natural Science Foundation of China
文摘Rapidly solidified Sn-9Zn-0.1Pr(/Nd) alloy foils were prepared by melt-spinning method. Through comparison, the effects of rapid solidification process and 0.1%Pr/Nd(mass fraction) addition on the microstructure, thermodynamic characteristic of Sn-9Zn solder alloy were analyzed. The tensile-shear tests were used to evaluate the mechanical properties of solder/Cu joints. The results show that the rapid solidification process can greatly refine the microstructure of Sn-9Zn-0.1Pr(/Nd) alloys. After rapid solidification, the effects of Pr/Nd addition on microstructure are depressed. The pasty range of the rapidly solidified Sn-Zn-RE solders is also reduced significantly. The mechanical properties of solder/Cu joints are obviously improved using the rapidly solidified Sn-9Zn-0.1Pr(/Nd) solder alloy, which results in the formation of uniform interface. The promotion effect of Nd addition in Sn-9Zn alloy on the interfacial reaction of solder/Cu joint is more remarkable than that of Pr.
基金the support from NSFC under Grant 61222105the 863 Plan of China under Grant 2014AA01A706+3 种基金the project of State Key Lab under Grant RCS2012ZT013the Key Project of Chinese Ministry of Education under Grant 313006the Key Project for Railway Ministry of China under Grant 2012X008-Athe project of State Key Lab under Grant No. RCS2011ZZ002
文摘With the fast development of highspeed railways,a call for fulfilling the notion of communication at "anytime,anywhere" for high-speed train passengers in the Train Operating Control System is on the way.In order to make a realization of that,new railway wireless communication networks are needed.The most promising one is the Long Term Evolution for Railway which will provide broadband access,fast handover,and reliable communication for high mobility users.However,with the increase of speed,the system is subjected to high bit error rate,Doppler frequency shift and handover failure just like other system does.This paper is trying to solve these problems by employing MIMO technique.Specifically,the goal is to provide higher data rate,higher reliability,less delay,and other relative quality of services for passengers.MIMO performance analysis,resource allocation,and access control for handover and various services in a two-hop model are proposed in this paper.Analytical results and simulation results show that the proposed model and schemes perform well in improving the system performances.
文摘Ubiquitous on earth,bacteriophages are the most abundant entities in every ecosystem,but human knowledge about them is still limited compared with that about other forms of organisms.To enrich human knowledge and promote the utilization of bacteriophages,it is necessary to isolate and characterize as many as possible different bacteriophages.Here we describe the isolation of a T4-like bacteriophage IME08 and a rapid method for its genetic characterization.With this method we easily cloned a few random fragments of the bacteriophage genome.Sequence analysis of these random clones showed that bacteriophage IME08 shared the highest sequence similarity with T4-like Enterobacteria phage T4(94%identity),JS98(95% identity),JS10(95%identity) and RB14(94%identity) respectively,which suggested that IME08 belonged to T4-like bacteriophage genus.
文摘On basis of two definitions that 1. an induced subgraph by a vertex vi E G and its neighbors in G is defined a vertex adjacent closed subgraph denoted by Qi (=G[V(Nvi)]), with the vertex vi called the hub; 2. A r(k,1)-1 vertices connected graph is called a (k,l)-Ramsey graph denoted by RG(k,l),if and only if 1. RG(k,l) contains only cliques of degree k-1, and its complement contains only cliques of degree l-l; 2. the intersect Qi∩Qj of any two nonadjacent vertices vi and vj of RG(k,1) contains Kk.2, and the intersect Qi∩Qj of any two nonadjacent vertices vi and vj of its complement RG(l,k) contains KI.2. Two theorems that theoreml : the biggest clique in G is contained in some Qi of G, and theorem2: r(k,l)= [ V(RG(k,I)) I +1 are put forward and proved in this paper. With those definitions and theorems as well as analysis of property of chords a method for quick inspection and building RG(k,I) is proposed. Accordingly, RG(10,3) and its complement are built, which are respectively the strongly 29-regular graph and the strongly 10-regular graph on orders 40. We have tested RG(10,3) and its complement RG(3,10),and gotten r(3,10)=41.
文摘Two definitions are given that Definitionl: an induced subgraph by a vertex vie G and its neighbors in G is defined as a vertex adjacent closed subgraph, and denoted by Qi (=G[V(Nvi)]), with the vertex vi called the hub; and Definition2: A r(k,I)-I vertices graph is called the (k,l)-Ramsey graph, denoted by RG(k,1), if RG(k,1) only contains cliques Kk.1 and the intersect QiNQj of any two nonadjacent vertices vi and vj of RG(k,I) contains only Kk-2. Meanwhile, the RG(k,l)'s complement RG(I,k) contains only cliques Kl.l, and the intersect QiNQj of any two nonadjacent vertices vi and vj of RG(I,k) contains only Ki.2. On the basis of those definitions, two theorems are put forward and proved in this paper. They are Theoreml: the biggest clique in G is contained in some Qi of G, and Theorem 2: r(k,1) = [V(RG(k,I))I + 1. With those definitions and theorems as well as analysis of chord property, a method for quick inspection and building RG(k,1) is proposed. Accordingly, RG(4,6) is built, it is a strongly 14-regular graph on order 35. We have tested RG(4,6) and its complement, as a result, they meet the defintion2, so we proclaim that r(4,6)=36.
基金the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry of Chinathe National Natural Science Foundation of China (No. 60802058)
文摘The paper proposes a robust digital audio watermarking scheme using blind source separation(BSS) based on the global optimization of independency metric(IM),which is formulated as a generalized eigenvalue(GE) problem.Compared with traditional information-theoretical approaches used in digital audio watermarking,such as fast independent component analysis(FastICA),the proposed scheme has lower complexity without timeconsuming iteration steps used in FastICA.To make full use of the multiresolution characteristic of discrete wavelet transform(DWT) and the energy compression characteristic of discrete cosine transform(DCT),the watermark is embedded in the middle DWT-DCT coefficients and the independent component analysis(ICA) approach based on IM is used in the detecting scheme.Simulation results based on Stirmark for Audio v02 show that the proposed scheme has strong robustness as well as the imperceptibility and security.