Ebola virus （EBOV） and Marburg virus （MARV） are causative agents of severe hemorrhagic fever with high mortality rates in humans and non-human primates and there is currently no licensed vaccine or therapeutics. T...Ebola virus （EBOV） and Marburg virus （MARV） are causative agents of severe hemorrhagic fever with high mortality rates in humans and non-human primates and there is currently no licensed vaccine or therapeutics. To date, there is no specific laboratory diagnostic test in China, while there is a national need to provide differential diagnosis during outbreaks and for instituting acceptable quarantine procedures. In this study, the TaqMan RT-PCR assays targeting the nucleoprotein genes of the Zaire Ebolavirus （ZEBOV） and MARV were developed and their sensitivities and specificities were investigated. Our results indicated that the assays were able to make reliable diagnosis over a wide range of virus copies from 103 to 109, corresponding to the threshold of a standard RNA transcript. The results showed that there were about 101 RNA copies per milliliter of virus culture supernatant, equivalent to 10,000 RNA molecules per infectious virion, suggesting the presence of many non-infectious particles. These data indicated that the TaqMan RT-PCR assays developed in this study will be suitable展开更多
On basis of two definitions that 1. an induced subgraph by a vertex vi E G and its neighbors in G is defined a vertex adjacent closed subgraph denoted by Qi （=G[V（Nvi）]）, with the vertex vi called the hub; 2. A r...On basis of two definitions that 1. an induced subgraph by a vertex vi E G and its neighbors in G is defined a vertex adjacent closed subgraph denoted by Qi （=G[V（Nvi）]）, with the vertex vi called the hub; 2. A r（k,1）-1 vertices connected graph is called a （k,l）-Ramsey graph denoted by RG（k,l）,if and only if 1. RG（k,l） contains only cliques of degree k-1, and its complement contains only cliques of degree l-l; 2. the intersect Qi∩Qj of any two nonadjacent vertices vi and vj of RG（k,1） contains Kk.2, and the intersect Qi∩Qj of any two nonadjacent vertices vi and vj of its complement RG（l,k） contains KI.2. Two theorems that theoreml ： the biggest clique in G is contained in some Qi of G, and theorem2： r（k,l）= [ V（RG（k,I）） I ＋1 are put forward and proved in this paper. With those definitions and theorems as well as analysis of property of chords a method for quick inspection and building RG（k,I） is proposed. Accordingly, RG（10,3） and its complement are built, which are respectively the strongly 29-regular graph and the strongly 10-regular graph on orders 40. We have tested RG（10,3） and its complement RG（3,10）,and gotten r（3,10）=41.展开更多
Two definitions are given that Definitionl： an induced subgraph by a vertex vie G and its neighbors in G is defined as a vertex adjacent closed subgraph, and denoted by Qi （=G[V（Nvi）]）, with the vertex vi called ...Two definitions are given that Definitionl： an induced subgraph by a vertex vie G and its neighbors in G is defined as a vertex adjacent closed subgraph, and denoted by Qi （=G[V（Nvi）]）, with the vertex vi called the hub; and Definition2： A r（k,I）-I vertices graph is called the （k,l）-Ramsey graph, denoted by RG（k,1）, if RG（k,1） only contains cliques Kk.1 and the intersect QiNQj of any two nonadjacent vertices vi and vj of RG（k,I） contains only Kk-2. Meanwhile, the RG（k,l）＇s complement RG（I,k） contains only cliques Kl.l, and the intersect QiNQj of any two nonadjacent vertices vi and vj of RG（I,k） contains only Ki.2. On the basis of those definitions, two theorems are put forward and proved in this paper. They are Theoreml： the biggest clique in G is contained in some Qi of G, and Theorem 2： r（k,1） = [V（RG（k,I））I ＋ 1. With those definitions and theorems as well as analysis of chord property, a method for quick inspection and building RG（k,1） is proposed. Accordingly, RG（4,6） is built, it is a strongly 14-regular graph on order 35. We have tested RG（4,6） and its complement, as a result, they meet the defintion2, so we proclaim that r（4,6）=36.展开更多
基金Supported by Important National Science&Technology Specific Projects(2009ZX10004-504,2009ZX09301-014)National Natural Science Foundation of China(81072675)
文摘Ebola virus （EBOV） and Marburg virus （MARV） are causative agents of severe hemorrhagic fever with high mortality rates in humans and non-human primates and there is currently no licensed vaccine or therapeutics. To date, there is no specific laboratory diagnostic test in China, while there is a national need to provide differential diagnosis during outbreaks and for instituting acceptable quarantine procedures. In this study, the TaqMan RT-PCR assays targeting the nucleoprotein genes of the Zaire Ebolavirus （ZEBOV） and MARV were developed and their sensitivities and specificities were investigated. Our results indicated that the assays were able to make reliable diagnosis over a wide range of virus copies from 103 to 109, corresponding to the threshold of a standard RNA transcript. The results showed that there were about 101 RNA copies per milliliter of virus culture supernatant, equivalent to 10,000 RNA molecules per infectious virion, suggesting the presence of many non-infectious particles. These data indicated that the TaqMan RT-PCR assays developed in this study will be suitable
文摘On basis of two definitions that 1. an induced subgraph by a vertex vi E G and its neighbors in G is defined a vertex adjacent closed subgraph denoted by Qi （=G[V（Nvi）]）, with the vertex vi called the hub; 2. A r（k,1）-1 vertices connected graph is called a （k,l）-Ramsey graph denoted by RG（k,l）,if and only if 1. RG（k,l） contains only cliques of degree k-1, and its complement contains only cliques of degree l-l; 2. the intersect Qi∩Qj of any two nonadjacent vertices vi and vj of RG（k,1） contains Kk.2, and the intersect Qi∩Qj of any two nonadjacent vertices vi and vj of its complement RG（l,k） contains KI.2. Two theorems that theoreml ： the biggest clique in G is contained in some Qi of G, and theorem2： r（k,l）= [ V（RG（k,I）） I ＋1 are put forward and proved in this paper. With those definitions and theorems as well as analysis of property of chords a method for quick inspection and building RG（k,I） is proposed. Accordingly, RG（10,3） and its complement are built, which are respectively the strongly 29-regular graph and the strongly 10-regular graph on orders 40. We have tested RG（10,3） and its complement RG（3,10）,and gotten r（3,10）=41.
文摘Two definitions are given that Definitionl： an induced subgraph by a vertex vie G and its neighbors in G is defined as a vertex adjacent closed subgraph, and denoted by Qi （=G[V（Nvi）]）, with the vertex vi called the hub; and Definition2： A r（k,I）-I vertices graph is called the （k,l）-Ramsey graph, denoted by RG（k,1）, if RG（k,1） only contains cliques Kk.1 and the intersect QiNQj of any two nonadjacent vertices vi and vj of RG（k,I） contains only Kk-2. Meanwhile, the RG（k,l）＇s complement RG（I,k） contains only cliques Kl.l, and the intersect QiNQj of any two nonadjacent vertices vi and vj of RG（I,k） contains only Ki.2. On the basis of those definitions, two theorems are put forward and proved in this paper. They are Theoreml： the biggest clique in G is contained in some Qi of G, and Theorem 2： r（k,1） = [V（RG（k,I））I ＋ 1. With those definitions and theorems as well as analysis of chord property, a method for quick inspection and building RG（k,1） is proposed. Accordingly, RG（4,6） is built, it is a strongly 14-regular graph on order 35. We have tested RG（4,6） and its complement, as a result, they meet the defintion2, so we proclaim that r（4,6）=36.
