A long procedure is needed to quarantine pinewood nematodes because only the dispersal third-stage larvae could be isolated and it cannot be identified directly before being cultured to adult nematodes. To shorten the...A long procedure is needed to quarantine pinewood nematodes because only the dispersal third-stage larvae could be isolated and it cannot be identified directly before being cultured to adult nematodes. To shorten the procedure, the effects of different temperatures and culture durations on the development of B. xylophilus larvae were studies with three fungus species as the culture media. The result showed that temperature had differential influence on development of the larvae in the three fungus media, and the influence was not significant. There was significantly different effect of the cold-stored fungi media on the larvae development. Fusarium sp. was the best, Pestalotia sp. was the second, Botrytis cinerea was the worst in promoting larvae development. Cold-stored B. cinerea was not suitable for the rapid quarantine of the nematode. When the L_Ⅲ larvae of B.xylophilus were abundant the quarantine could be accomplished in 48 h by culturing the L_Ⅲ larvae with cold-stored Fusarium sp. or Pestalotia sp. at 30展开更多
对江苏、安徽 2疫区的黑松、赤松、马尾松、火炬松、湿地松等 5个树种松材线虫病 Bursaphe-lenchus xylophilus的病、健木 p H值差异性分析结果表明 :感病品种黑松、赤松、马尾松病木 p H值降低 ,且差异显著 ;较抗病的湿地松虽然也降低 ...对江苏、安徽 2疫区的黑松、赤松、马尾松、火炬松、湿地松等 5个树种松材线虫病 Bursaphe-lenchus xylophilus的病、健木 p H值差异性分析结果表明 :感病品种黑松、赤松、马尾松病木 p H值降低 ,且差异显著 ;较抗病的湿地松虽然也降低 ,但差异不显著 ;抗病性强的火炬松病木 p H值反而高于健木 ,且差异显著。由此可见 ,不同松树品种松材线虫病的病、健木 p H值的差异性也不同 ,对松材线虫越敏感和受害越严重的 ,其病木 p H值降低的就越多 ,与健木 p H值差异就越显著。该分析结果初步为我国松材线虫为害比较严重的几种主要松树品种 。展开更多
文摘A long procedure is needed to quarantine pinewood nematodes because only the dispersal third-stage larvae could be isolated and it cannot be identified directly before being cultured to adult nematodes. To shorten the procedure, the effects of different temperatures and culture durations on the development of B. xylophilus larvae were studies with three fungus species as the culture media. The result showed that temperature had differential influence on development of the larvae in the three fungus media, and the influence was not significant. There was significantly different effect of the cold-stored fungi media on the larvae development. Fusarium sp. was the best, Pestalotia sp. was the second, Botrytis cinerea was the worst in promoting larvae development. Cold-stored B. cinerea was not suitable for the rapid quarantine of the nematode. When the L_Ⅲ larvae of B.xylophilus were abundant the quarantine could be accomplished in 48 h by culturing the L_Ⅲ larvae with cold-stored Fusarium sp. or Pestalotia sp. at 30
文摘对江苏、安徽 2疫区的黑松、赤松、马尾松、火炬松、湿地松等 5个树种松材线虫病 Bursaphe-lenchus xylophilus的病、健木 p H值差异性分析结果表明 :感病品种黑松、赤松、马尾松病木 p H值降低 ,且差异显著 ;较抗病的湿地松虽然也降低 ,但差异不显著 ;抗病性强的火炬松病木 p H值反而高于健木 ,且差异显著。由此可见 ,不同松树品种松材线虫病的病、健木 p H值的差异性也不同 ,对松材线虫越敏感和受害越严重的 ,其病木 p H值降低的就越多 ,与健木 p H值差异就越显著。该分析结果初步为我国松材线虫为害比较严重的几种主要松树品种 。
