WT8.BZ]The effects of postgrowth rapid thermal annealing have been studied on the optical properties of 3-nm-height InAs/GaAs quantum dots covered by 3-nm-thick In xGa 1-x As (x=0,0 1 and 0 2) overgrowth layer...WT8.BZ]The effects of postgrowth rapid thermal annealing have been studied on the optical properties of 3-nm-height InAs/GaAs quantum dots covered by 3-nm-thick In xGa 1-x As (x=0,0 1 and 0 2) overgrowth layer.At a higher annealing temperature (T≥750℃),the photoluminescence peak of InGaAs layer has been observed at the lower-energy side of InAs quantum-dot peak.In addition,a similar blueshift in photoluminescence (PL) emission energy is observed for all samples when the annealing temperature increases from 650 to 850℃.However,the trend of photoluminescence linewidth towards narrowing is totally different for InAs quantum dots with different In mole fraction in InGaAs overgrowth layer.The results suggest that the intermixing in the lateral direction plays an important role in obtaining a better understanding of the modification of optical properties induced by the rapid thermal annealing.展开更多
The urban population and urbanized land in China have both increased markedly since the 1980 s. Urban and suburban developments have grown at unprecedented rates with unknown consequences for ecosystem functions. In p...The urban population and urbanized land in China have both increased markedly since the 1980 s. Urban and suburban developments have grown at unprecedented rates with unknown consequences for ecosystem functions. In particular, the effect of rapid urbanization on the storage of soil carbon has not been studied extensively. In this study, we compared the soil carbon stocks of different land use types in Beijing Municipality. We collected 490 top-soil samples(top 20 cm) from urban and suburban sites within the Sixth Ring Road of Beijing, which cover approximately 2400 km2, and the densities of soil organic carbon(SOC), soil inorganic carbon(SIC), and total carbon(TC) were analyzed to determine the spatial distribution of urban and suburban soil carbon characteristics across seven land use types. The results revealed significant differences in soil carbon densities among land use types. Additionally, urban soil had significantly higher SOC and SIC densities than suburban soil did, and suburban shelterbelts and productive plantations had lower SIC densities than the other land use types. The comparison of coefficients of variance(CVs) showed that carbon content of urban topsoil had a lower variability than that of suburban topsoil. Further findings revealed that soil carbon storage increased with built-up age. Urban soil built up for more than 20 years had higher densities of SOC, SIC and TC than both urban soil with less than 10 years and suburban soil. Correlation analyses indicated the existence of a significantly negative correlation between the SOC, SIC, and TC densities of urban soil and the distance to the urban core, and the distance variable alone explained 23.3% of the variation of SIC density and 13.8% of the variation of TC density. These results indicate that SOC and SIC accumulate in the urban topsoil under green space as a result of the conversion of agricultural land to urban land due to the urbanization in Beijing.展开更多
The hallmark of development in the Yangtze River Delta(YRD) of East China has been sprawling urbanization. However, air pollution is a significant problem in these urban areas. In this paper, we investigated and analy...The hallmark of development in the Yangtze River Delta(YRD) of East China has been sprawling urbanization. However, air pollution is a significant problem in these urban areas. In this paper, we investigated and analyzed the air pollution index(API) in four cities(Shanghai, Nanjing, Hangzhou and Ningbo) in the YRD from 2001 to 2012. We attempted to empirically examine the relationship between meteorological factors and air quality in the urban areas of the YRD. According to the monitoring data, the API in Shanghai, Nanjing, Hangzhou slightly declined and that in Ningbo increased over the study period. We analyzed the inter-annual, seasonal, and monthly variations of API, from which we found that the air quality had different temporal changes in the four cities. It was indicated that air quality was poor in winter and spring and best in summer. Furthermore, different weather conditions affected air quality level. The wind direction was considered as an important and influential factor to air pollution, which has an impact on the accumulating or cleaning processes of pollutants. The air quality was influenced by the different wind directions that varied with seasons and cities.展开更多
The complicated life cycle ofAurelia spp., comprising benthic asexually-reproducing polyps and sexually-reproducing medusae, makes it hard for researchers to identify and track them, especially for early stage individ...The complicated life cycle ofAurelia spp., comprising benthic asexually-reproducing polyps and sexually-reproducing medusae, makes it hard for researchers to identify and track them, especially for early stage individuals, such as planulae. To solve this problem, we developed a real-time PCR assay (SYBR Green I) to identify planulae in both cultured and natural seawater samples. Species-specific primers targeting Aurelia sp.1 mitochondrial 16S rDNA (mr 16S rDNA) regions were designed. Using a calibration curve constructed with plasmids containing the Aurelia sp. 1 mt 16S rDNA fragment and a standard curve for planulae, the absolute number of mt 16S rDNA copies per planula was determined and from that the total number ofplanulae per sample was estimated. For the field samples, a 100-fold dilution of the sample DNA combined with a final concentration of 0.2 μg/μL BSA in the PCR reaction mixture was used to remove real- time PCR inhibitors. Samples collected in Jiaozhou Bay from July to September 2012 were subsequently analyzed using this assay. Peak Aurelia sp.1 planula abundance occurred in July 2012 at stations near Hongdao Island and Qingdao offshore; abundances were very low in August and September. The real-time PCR assay (SYBR Green I) developed here negates the need for traditional microscopic identification, which is laborious and time-consuming, and can detect and quantify jellyfish planulae in field plankton samples rapidly and specifically.展开更多
The observed meridional overtuming circula- tion (MOC) and meridional heat transport (MHT) estimated from the Rapid Climate Change/Meridional Circu- lation and Heat Flux Array (RAPID/MOCHA) at 26.5°N are us...The observed meridional overtuming circula- tion (MOC) and meridional heat transport (MHT) estimated from the Rapid Climate Change/Meridional Circu- lation and Heat Flux Array (RAPID/MOCHA) at 26.5°N are used to evaluate the volume and heat transport in the eddy-resolving model LASG/IAP Climate system Ocean Model (LICOM). The authors find that the Florida Cur- rent transport and upper mid-ocean transport of the model are underestimated against the observations. The simulated variability of MOC and MHT show a high correlation with the observations, exceeding 0.6. Both the simulated and observed MOC and MHT show a significant seasonal variability. According to the power spectrum analysis, LICOM can represent the mesoscale eddy characteristic of the MOC similar to the observation. The model shows a high correlation of 0.58 for the internal upper mid-ocean transport (MO) and a density difference between the western and eastern boundaries, as noted in previous studies.展开更多
Detection of sulfur-oxidizing bacteria has largely been dependent on targeted gene sequencing technology or traditional cell cultivation, which usually takes from days to months to carry out. This clearly does not mee...Detection of sulfur-oxidizing bacteria has largely been dependent on targeted gene sequencing technology or traditional cell cultivation, which usually takes from days to months to carry out. This clearly does not meet the requirements of analysis for time-sensitive samples and/or complicated environmental samples. Since energy-dispersive X-ray spectrometry(EDS) can be used to simultaneously detect multiple elements in a sample, including sulfur, with minimal sample treatment, this technology was applied to detect sulfur-oxidizing bacteria using their high sulfur content within the cell. This article describes the application of scanning electron microscopy imaging coupled with EDS mapping for quick detection of sulfur oxidizers in contaminated environmental water samples, with minimal sample handling. Scanning electron microscopy imaging revealed the existence of dense granules within the bacterial cells, while EDS identified large amounts of sulfur within them. EDS mapping localized the sulfur to these granules. Subsequent 16S rRNA gene sequencing showed that the bacteria detected in our samples belonged to the genus Chromatium, which are sulfur oxidizers. Thus, EDS mapping made it possible to identify sulfur oxidizers in environmental samples based on localized sulfur within their cells, within a short time(within 24 h of sampling). This technique has wide ranging applications for detection of sulfur bacteria in environmental water samples.展开更多
The paper proposes a robust digital audio watermarking scheme using blind source separation(BSS) based on the global optimization of independency metric(IM),which is formulated as a generalized eigenvalue(GE) problem....The paper proposes a robust digital audio watermarking scheme using blind source separation(BSS) based on the global optimization of independency metric(IM),which is formulated as a generalized eigenvalue(GE) problem.Compared with traditional information-theoretical approaches used in digital audio watermarking,such as fast independent component analysis(FastICA),the proposed scheme has lower complexity without timeconsuming iteration steps used in FastICA.To make full use of the multiresolution characteristic of discrete wavelet transform(DWT) and the energy compression characteristic of discrete cosine transform(DCT),the watermark is embedded in the middle DWT-DCT coefficients and the independent component analysis(ICA) approach based on IM is used in the detecting scheme.Simulation results based on Stirmark for Audio v02 show that the proposed scheme has strong robustness as well as the imperceptibility and security.展开更多
Rapid diagnosis is important for efficient treatment in clinical medicine.This study aimed at development of a method for rapid and reliable diagnosis using near-infrared(NIR)spectra of human serum samples with the he...Rapid diagnosis is important for efficient treatment in clinical medicine.This study aimed at development of a method for rapid and reliable diagnosis using near-infrared(NIR)spectra of human serum samples with the help of chemometric modelling.The NIR spectra of sera from 48 healthy individuals and 16 patients with suspected kidney disease were analyzed.Discrete wavelet transform(DWT)and variable selection were adopted to extract the useful information from the spectra.Principal component analysis(PCA),linear discriminant analysis(LDA)and partial least squares discriminant analysis(PLSDA)were used for discrimination of the samples.Classification of the two-class sera was obtained using LDA and PLSDA with the help of DWT and variable selection.DWT-LDA produced 93.8%and 83.3%of the recognition rates for the validation samples of the two classes,and 100%recognition rates were obtained using DWT-PLSDA.The results demonstrated that the tiny differences between the spectra of the sera were effectively explored using DWT and variable selection,and the differences can be used for discrimination of the sera from healthy and possible patients.NIR spectroscopy and chemometrics may be a potential technique for fast diagnosis of kidney disease.展开更多
文摘WT8.BZ]The effects of postgrowth rapid thermal annealing have been studied on the optical properties of 3-nm-height InAs/GaAs quantum dots covered by 3-nm-thick In xGa 1-x As (x=0,0 1 and 0 2) overgrowth layer.At a higher annealing temperature (T≥750℃),the photoluminescence peak of InGaAs layer has been observed at the lower-energy side of InAs quantum-dot peak.In addition,a similar blueshift in photoluminescence (PL) emission energy is observed for all samples when the annealing temperature increases from 650 to 850℃.However,the trend of photoluminescence linewidth towards narrowing is totally different for InAs quantum dots with different In mole fraction in InGaAs overgrowth layer.The results suggest that the intermixing in the lateral direction plays an important role in obtaining a better understanding of the modification of optical properties induced by the rapid thermal annealing.
基金Under the auspices of National Key Technology Research and Development Program(No.2007BAC28B01)Innovation Project of State Key Laboratory of Urban and Regional Ecology of China
文摘The urban population and urbanized land in China have both increased markedly since the 1980 s. Urban and suburban developments have grown at unprecedented rates with unknown consequences for ecosystem functions. In particular, the effect of rapid urbanization on the storage of soil carbon has not been studied extensively. In this study, we compared the soil carbon stocks of different land use types in Beijing Municipality. We collected 490 top-soil samples(top 20 cm) from urban and suburban sites within the Sixth Ring Road of Beijing, which cover approximately 2400 km2, and the densities of soil organic carbon(SOC), soil inorganic carbon(SIC), and total carbon(TC) were analyzed to determine the spatial distribution of urban and suburban soil carbon characteristics across seven land use types. The results revealed significant differences in soil carbon densities among land use types. Additionally, urban soil had significantly higher SOC and SIC densities than suburban soil did, and suburban shelterbelts and productive plantations had lower SIC densities than the other land use types. The comparison of coefficients of variance(CVs) showed that carbon content of urban topsoil had a lower variability than that of suburban topsoil. Further findings revealed that soil carbon storage increased with built-up age. Urban soil built up for more than 20 years had higher densities of SOC, SIC and TC than both urban soil with less than 10 years and suburban soil. Correlation analyses indicated the existence of a significantly negative correlation between the SOC, SIC, and TC densities of urban soil and the distance to the urban core, and the distance variable alone explained 23.3% of the variation of SIC density and 13.8% of the variation of TC density. These results indicate that SOC and SIC accumulate in the urban topsoil under green space as a result of the conversion of agricultural land to urban land due to the urbanization in Beijing.
基金Under the auspices of Special Research Fund of the Ministry of Land and Resources for the Non-Profit Sector(No201411014-03)National Key Technology Research and Development Program of China(No.2012BAH28B04)
文摘The hallmark of development in the Yangtze River Delta(YRD) of East China has been sprawling urbanization. However, air pollution is a significant problem in these urban areas. In this paper, we investigated and analyzed the air pollution index(API) in four cities(Shanghai, Nanjing, Hangzhou and Ningbo) in the YRD from 2001 to 2012. We attempted to empirically examine the relationship between meteorological factors and air quality in the urban areas of the YRD. According to the monitoring data, the API in Shanghai, Nanjing, Hangzhou slightly declined and that in Ningbo increased over the study period. We analyzed the inter-annual, seasonal, and monthly variations of API, from which we found that the air quality had different temporal changes in the four cities. It was indicated that air quality was poor in winter and spring and best in summer. Furthermore, different weather conditions affected air quality level. The wind direction was considered as an important and influential factor to air pollution, which has an impact on the accumulating or cleaning processes of pollutants. The air quality was influenced by the different wind directions that varied with seasons and cities.
基金Supported by the National Basic Research Program of China(973 Program)(No.2011CB403602)the National Natural Science Foundation of China(No.41076085)the National Special Research Fund for Non-Profit Marine Sector(No.201205031)
文摘The complicated life cycle ofAurelia spp., comprising benthic asexually-reproducing polyps and sexually-reproducing medusae, makes it hard for researchers to identify and track them, especially for early stage individuals, such as planulae. To solve this problem, we developed a real-time PCR assay (SYBR Green I) to identify planulae in both cultured and natural seawater samples. Species-specific primers targeting Aurelia sp.1 mitochondrial 16S rDNA (mr 16S rDNA) regions were designed. Using a calibration curve constructed with plasmids containing the Aurelia sp. 1 mt 16S rDNA fragment and a standard curve for planulae, the absolute number of mt 16S rDNA copies per planula was determined and from that the total number ofplanulae per sample was estimated. For the field samples, a 100-fold dilution of the sample DNA combined with a final concentration of 0.2 μg/μL BSA in the PCR reaction mixture was used to remove real- time PCR inhibitors. Samples collected in Jiaozhou Bay from July to September 2012 were subsequently analyzed using this assay. Peak Aurelia sp.1 planula abundance occurred in July 2012 at stations near Hongdao Island and Qingdao offshore; abundances were very low in August and September. The real-time PCR assay (SYBR Green I) developed here negates the need for traditional microscopic identification, which is laborious and time-consuming, and can detect and quantify jellyfish planulae in field plankton samples rapidly and specifically.
基金jointly supported by the National Basic Research Program of China (Grant No. 2010CB950502)"Strategic Priority Research Program-Climate Change: Carbon Budget and Related Issues" of the Chinese Academy of Sciences(Grant No. XDA05110302)+2 种基金the National High Technology Research and Development Program of China (863 Program, Grant No.2010AA012304)the National Natural Science Foundation of China (Grant No. 40975065)Data from the RAPID-MOCHA program are funded by the U.S. National Science Foundation
文摘The observed meridional overtuming circula- tion (MOC) and meridional heat transport (MHT) estimated from the Rapid Climate Change/Meridional Circu- lation and Heat Flux Array (RAPID/MOCHA) at 26.5°N are used to evaluate the volume and heat transport in the eddy-resolving model LASG/IAP Climate system Ocean Model (LICOM). The authors find that the Florida Cur- rent transport and upper mid-ocean transport of the model are underestimated against the observations. The simulated variability of MOC and MHT show a high correlation with the observations, exceeding 0.6. Both the simulated and observed MOC and MHT show a significant seasonal variability. According to the power spectrum analysis, LICOM can represent the mesoscale eddy characteristic of the MOC similar to the observation. The model shows a high correlation of 0.58 for the internal upper mid-ocean transport (MO) and a density difference between the western and eastern boundaries, as noted in previous studies.
基金Supported by the Basic Scientific Fund for National Public Research Institutes of China(Nos.GY02-2011T10,2015P07)the Qingdao Talent Program(No.13-CX-20)+1 种基金the National Natural Science Foundation of China(Nos.31100567,41176061)the National Natural Science Foundation for Creative Groups(No.41521064)
文摘Detection of sulfur-oxidizing bacteria has largely been dependent on targeted gene sequencing technology or traditional cell cultivation, which usually takes from days to months to carry out. This clearly does not meet the requirements of analysis for time-sensitive samples and/or complicated environmental samples. Since energy-dispersive X-ray spectrometry(EDS) can be used to simultaneously detect multiple elements in a sample, including sulfur, with minimal sample treatment, this technology was applied to detect sulfur-oxidizing bacteria using their high sulfur content within the cell. This article describes the application of scanning electron microscopy imaging coupled with EDS mapping for quick detection of sulfur oxidizers in contaminated environmental water samples, with minimal sample handling. Scanning electron microscopy imaging revealed the existence of dense granules within the bacterial cells, while EDS identified large amounts of sulfur within them. EDS mapping localized the sulfur to these granules. Subsequent 16S rRNA gene sequencing showed that the bacteria detected in our samples belonged to the genus Chromatium, which are sulfur oxidizers. Thus, EDS mapping made it possible to identify sulfur oxidizers in environmental samples based on localized sulfur within their cells, within a short time(within 24 h of sampling). This technique has wide ranging applications for detection of sulfur bacteria in environmental water samples.
基金the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry of Chinathe National Natural Science Foundation of China (No. 60802058)
文摘The paper proposes a robust digital audio watermarking scheme using blind source separation(BSS) based on the global optimization of independency metric(IM),which is formulated as a generalized eigenvalue(GE) problem.Compared with traditional information-theoretical approaches used in digital audio watermarking,such as fast independent component analysis(FastICA),the proposed scheme has lower complexity without timeconsuming iteration steps used in FastICA.To make full use of the multiresolution characteristic of discrete wavelet transform(DWT) and the energy compression characteristic of discrete cosine transform(DCT),the watermark is embedded in the middle DWT-DCT coefficients and the independent component analysis(ICA) approach based on IM is used in the detecting scheme.Simulation results based on Stirmark for Audio v02 show that the proposed scheme has strong robustness as well as the imperceptibility and security.
基金supported by the National Natural Science Foundation of China(21475068)MOE Innovation Team (IRT13022) of China
文摘Rapid diagnosis is important for efficient treatment in clinical medicine.This study aimed at development of a method for rapid and reliable diagnosis using near-infrared(NIR)spectra of human serum samples with the help of chemometric modelling.The NIR spectra of sera from 48 healthy individuals and 16 patients with suspected kidney disease were analyzed.Discrete wavelet transform(DWT)and variable selection were adopted to extract the useful information from the spectra.Principal component analysis(PCA),linear discriminant analysis(LDA)and partial least squares discriminant analysis(PLSDA)were used for discrimination of the samples.Classification of the two-class sera was obtained using LDA and PLSDA with the help of DWT and variable selection.DWT-LDA produced 93.8%and 83.3%of the recognition rates for the validation samples of the two classes,and 100%recognition rates were obtained using DWT-PLSDA.The results demonstrated that the tiny differences between the spectra of the sera were effectively explored using DWT and variable selection,and the differences can be used for discrimination of the sera from healthy and possible patients.NIR spectroscopy and chemometrics may be a potential technique for fast diagnosis of kidney disease.