【目的】青田田鱼在浅水环境中经常面临急性低温胁迫,探究青田田鱼在急性低温和复温环境下的生理响应机制,为青田田鱼科学越冬和耐低温品种选育提供理论依据。【方法】通过液相色谱—质谱(LC-MS)非靶向代谢组学技术,提取9.0℃胁迫6 h(CO...【目的】青田田鱼在浅水环境中经常面临急性低温胁迫,探究青田田鱼在急性低温和复温环境下的生理响应机制,为青田田鱼科学越冬和耐低温品种选育提供理论依据。【方法】通过液相色谱—质谱(LC-MS)非靶向代谢组学技术,提取9.0℃胁迫6 h(CO组)、升温至28.0℃复温恢复6 h(RE组)和28.0℃对照组(Con组)青田田鱼鳃组织样本代谢峰。预处理后的代谢组数据与数据库匹配鉴定代谢物种类。通过主成分分析(PCA)和正交最小偏二乘判别分析(OPLS-DA)鉴定代谢组学数据的可靠性。根据Student’s test检验的P值(P<0.05)和OPLS-DA模型得到的变量权重值(VIP>1)筛选组间差异代谢物。对差异代谢物进行KEGG信号通路富集分析,并用Fisher精确检验分析筛选与急性低温和复温最相关的代谢通路。对重要代谢通路制作聚类热图,显示组间差异和代谢物水平变化趋势。【结果】PCA和OPLS-DA分析结果显示,组内样本聚集,组间样本分离,组间的代谢物水平存在显著差异。OPLS-DA得分图显示各组Q2累计值均在0.5以上,R2Y累积值均接近1.0,模型具有较高的解释度和可靠性;共鉴定到1222个代谢物,其中CO vs Con,RE vs CO和RE vs Con分别筛选鉴定出232、238和300种显著差异代谢物(P<0.05)。各组间差异代谢物主要富集在花生四烯酸代谢、甘油磷脂代谢、类固醇激素生物合成和嘌呤代谢等代谢途径;急性低温胁迫下,皮质醇水平上调,而花生四烯酸及其代谢物和甘油磷脂代谢物下调。复温后花生四烯酸、甘油磷脂代谢物和嘌呤代谢物相对Con组下调,皮质醇含量下调,牛磺酸含量上调。【结论】急性低温可导致青田田鱼免疫、物质转运和信号传递等功能异常。皮质醇的合成和代谢可能是青田田鱼抵抗急性低温胁迫的重要机制。复温后青田田鱼鳃组织损伤在短期内无法得到完全恢复。牛磺酸合成可能是青田田鱼在复温过程中的重要恢复机制。展开更多
为探究急性低温胁迫对子二代中华鲟抗氧化、免疫及消化酶活性的影响,将健康子二代中华鲟[体质量(1.72±0.33) kg]由23 ℃水体中直接转入17、11 ℃水体中,急性胁迫2 h后取样,检测各项指标。试验结果显示,血清抗氧化指标中,超氧化物...为探究急性低温胁迫对子二代中华鲟抗氧化、免疫及消化酶活性的影响,将健康子二代中华鲟[体质量(1.72±0.33) kg]由23 ℃水体中直接转入17、11 ℃水体中,急性胁迫2 h后取样,检测各项指标。试验结果显示,血清抗氧化指标中,超氧化物歧化酶、过氧化氢酶活性随水温骤降温差增大,呈先升后降的趋势;而谷胱甘肽、丙二醛含量则呈先降后升的趋势( P >0.05);总抗氧化能力逐渐升高。肝脏抗氧化指标中,超氧化物歧化酶和过氧化氢酶活性均呈先升后降的趋势;谷胱甘肽含量逐渐下降,而丙二醛含量却显著增加( P <0.05);总抗氧化能力出现波动( P >0.05)。免疫指标中,血清和肝脏溶菌酶活性随水温骤降幅度的增大,均呈先升后降的趋势,而免疫球蛋白M含量递减( P >0.05)。消化酶指标中,胃、肠和肝脏淀粉酶活性随水温骤降温差的增大,均不断下降;胃、肝脏的脂肪酶活性却持续显著增加( P <0.05);胃蛋白酶活性则出现先降后升的趋势( P <0.05)。试验结果表明,急性低温胁迫对子二代中华鲟的抗氧化、免疫和消化酶活性均产生了较大影响,并对其肝脏产生了一定程度的不利影响。因此,在中华鲟的养殖、运输及人工放流过程中,应尽量避免水温骤降造成鱼体损伤。展开更多
The objective of the study was to explore the effect of acute low temperature stress on VO2 and Vf of Silurus meridionalis and Pelteobag vachelli after 10 minutes cold water bath with different temperature.The investi...The objective of the study was to explore the effect of acute low temperature stress on VO2 and Vf of Silurus meridionalis and Pelteobag vachelli after 10 minutes cold water bath with different temperature.The investigation was operated under the temperature of 24 ℃.It was found that the VO2 and Vf of Silurus meridionalis after 6 and 0 ℃ stress showed a decrease-increase-decrease trend while other groups showed a rapid increase then slowly recovery trend.The VO2 and Vf of Pelteobag vachelli after 0 ℃stress showed a decrease-increase-decrease trend while other groups showed an increase then slowly recovery process.It was suggested that Pelteobag vachelli was more adaptive to acute cold stress,but it cost more energy adapting to cold stress compared to Silurus meridionalis.展开更多
AIM： To investigate the relationship between donor liver cold preservation, lung surfactant （LS） changes and acute lung injury （ALI） after liver transplantation. METHODS： Liver transplantation models were estab-...AIM： To investigate the relationship between donor liver cold preservation, lung surfactant （LS） changes and acute lung injury （ALI） after liver transplantation. METHODS： Liver transplantation models were estab- lished using male Wistar rats. Donor livers were pre- served in University of Wisconsin solution at 4 ℃ for different lengths of time. The effect of ammonium pyr- rolidinedithiocarbamate （PDTC） on ALI was also detect- ed. All samples were harvested after 3 h reperfusion.The severity of AU was evaluated by lung weight/body weight ratio, lung histopathological score, serum nitric oxide （NO） and endothelin （ET）-I levels, lung tumor necrosis factor （TNF）-α and interleukin （IL）-1β levels. Lung surfactants （LSs） were determined by micellar electrokinetic capillary chromatography. RESULTS： With extended donor liver cold preservation time （CPT）, lung histopathological scores, serum ET-i levels, lung weight/body weight ratio and the level of TNF-α and IL-1β in lung were increased significantly in the 180-min group compared with the sham group （3.16± 0.28 vs 1.12 ± 0.21, P 〈 0.001; 343.59±53.97 vs 141.53± 48.48, P 〈 0.001; 0.00687 ±0.00037 vs 0.00557 ±0.00056, P 〈 0.001; 17.5 ± 3.0 vs 1,3 ± 0.3, P 〈 0.001; 10.8± 2.3 vs 1.8 ± 0.4, P 〈 0.001）, but serum NO levels decreased remarkably （74.67 ± 10.01 vs 24.97 ± 3.18, P 〈 0.001）. The expression of lung phos- phatidylcholine （PC）, phosphatidylethanolamine （PE）, phosphatidylinositol （PI） and phosphatidylserine （PS） increased when CPT was 〈 120 rain, and decreased when CPT was 〉 180 min （PC： 1318.89 ± 54.79 vs 1011.18± 59.99, P 〈 0.001; PE： 1504.45± 119.96 vs 1340.80±76.39, P = 0.0019; PI： 201.23 ± 34.82 vs 185.88 ± 17.04, P = 0.2265; PS： 300.43±32.95 vs 286.55± 55.55, P = 0.5054）. All these ALI-associated indexes could be partially reversed by PDTC treatment.展开更多
To observe the changes of evoked potentials after severe brain injury and the effect of mild hypothermia on acute severe brain injury. Methods: A total of 44 patients with severe closed head injury (GCS 3 8, admitted ...To observe the changes of evoked potentials after severe brain injury and the effect of mild hypothermia on acute severe brain injury. Methods: A total of 44 patients with severe closed head injury (GCS 3 8, admitted within 10 hours from injury) admitted from May 1998 to March 1999 were selected for this study. All patients were admitted into the intensive care unit and divided into 2 groups, Group A (GCS 3 5) and Group B (GCS 6 8). Patients were also randomly assigned to either normothermia or hypothermia subgroups. Patients in the hypothermia group were cooled to 32 34℃. Median nerve short latency somatosensory evoked potentials (SLSEP) and brain stem auditory evoked potentials (BAEP) were recorded before cooling and 4, 24, 48, 72, 96 and 120 hours, respectively after cooling and temperature resuming. SLSEP and BAEP were measured at the same time in the normothermia group (control group). The changes of evoked potentials (EP) were analyzed by statistical methods. Results: In the Group B, N 20 amplitudes in SLSEP and I/V amplitudes in BAEP after mild hypothermia treatment in the hypothermia group differed significantly from those in the control group (P< 0.05 ). However, in the Group A, no significant difference in all parameters was found. Conclusions: These results demonstrate that mild hypothermia treatment (32 34℃) in the Group B has a significant neuroelectrophysiological effect on severe brain injury. Nevertheless, the effect of mild hypothermia in the Group A is not apparent and needs further studying.展开更多
文摘【目的】青田田鱼在浅水环境中经常面临急性低温胁迫,探究青田田鱼在急性低温和复温环境下的生理响应机制,为青田田鱼科学越冬和耐低温品种选育提供理论依据。【方法】通过液相色谱—质谱(LC-MS)非靶向代谢组学技术,提取9.0℃胁迫6 h(CO组)、升温至28.0℃复温恢复6 h(RE组)和28.0℃对照组(Con组)青田田鱼鳃组织样本代谢峰。预处理后的代谢组数据与数据库匹配鉴定代谢物种类。通过主成分分析(PCA)和正交最小偏二乘判别分析(OPLS-DA)鉴定代谢组学数据的可靠性。根据Student’s test检验的P值(P<0.05)和OPLS-DA模型得到的变量权重值(VIP>1)筛选组间差异代谢物。对差异代谢物进行KEGG信号通路富集分析,并用Fisher精确检验分析筛选与急性低温和复温最相关的代谢通路。对重要代谢通路制作聚类热图,显示组间差异和代谢物水平变化趋势。【结果】PCA和OPLS-DA分析结果显示,组内样本聚集,组间样本分离,组间的代谢物水平存在显著差异。OPLS-DA得分图显示各组Q2累计值均在0.5以上,R2Y累积值均接近1.0,模型具有较高的解释度和可靠性;共鉴定到1222个代谢物,其中CO vs Con,RE vs CO和RE vs Con分别筛选鉴定出232、238和300种显著差异代谢物(P<0.05)。各组间差异代谢物主要富集在花生四烯酸代谢、甘油磷脂代谢、类固醇激素生物合成和嘌呤代谢等代谢途径;急性低温胁迫下,皮质醇水平上调,而花生四烯酸及其代谢物和甘油磷脂代谢物下调。复温后花生四烯酸、甘油磷脂代谢物和嘌呤代谢物相对Con组下调,皮质醇含量下调,牛磺酸含量上调。【结论】急性低温可导致青田田鱼免疫、物质转运和信号传递等功能异常。皮质醇的合成和代谢可能是青田田鱼抵抗急性低温胁迫的重要机制。复温后青田田鱼鳃组织损伤在短期内无法得到完全恢复。牛磺酸合成可能是青田田鱼在复温过程中的重要恢复机制。
文摘为探究急性低温胁迫对子二代中华鲟抗氧化、免疫及消化酶活性的影响,将健康子二代中华鲟[体质量(1.72±0.33) kg]由23 ℃水体中直接转入17、11 ℃水体中,急性胁迫2 h后取样,检测各项指标。试验结果显示,血清抗氧化指标中,超氧化物歧化酶、过氧化氢酶活性随水温骤降温差增大,呈先升后降的趋势;而谷胱甘肽、丙二醛含量则呈先降后升的趋势( P >0.05);总抗氧化能力逐渐升高。肝脏抗氧化指标中,超氧化物歧化酶和过氧化氢酶活性均呈先升后降的趋势;谷胱甘肽含量逐渐下降,而丙二醛含量却显著增加( P <0.05);总抗氧化能力出现波动( P >0.05)。免疫指标中,血清和肝脏溶菌酶活性随水温骤降幅度的增大,均呈先升后降的趋势,而免疫球蛋白M含量递减( P >0.05)。消化酶指标中,胃、肠和肝脏淀粉酶活性随水温骤降温差的增大,均不断下降;胃、肝脏的脂肪酶活性却持续显著增加( P <0.05);胃蛋白酶活性则出现先降后升的趋势( P <0.05)。试验结果表明,急性低温胁迫对子二代中华鲟的抗氧化、免疫和消化酶活性均产生了较大影响,并对其肝脏产生了一定程度的不利影响。因此,在中华鲟的养殖、运输及人工放流过程中,应尽量避免水温骤降造成鱼体损伤。
基金Supported by the National Natural Science Foundation of China(30371121)the Natural Science Foundation of Chongqing the Educa-tion Committee Foundation of Chongqing~~
文摘The objective of the study was to explore the effect of acute low temperature stress on VO2 and Vf of Silurus meridionalis and Pelteobag vachelli after 10 minutes cold water bath with different temperature.The investigation was operated under the temperature of 24 ℃.It was found that the VO2 and Vf of Silurus meridionalis after 6 and 0 ℃ stress showed a decrease-increase-decrease trend while other groups showed a rapid increase then slowly recovery trend.The VO2 and Vf of Pelteobag vachelli after 0 ℃stress showed a decrease-increase-decrease trend while other groups showed an increase then slowly recovery process.It was suggested that Pelteobag vachelli was more adaptive to acute cold stress,but it cost more energy adapting to cold stress compared to Silurus meridionalis.
基金Supported by Grants from the National Natural Science Foundation of China,No.30200269Science and technology projects in Shannxi Province,No.2006k12-G3(6)Science and technology projects in Xi’an city,No.SF1025(3)
文摘AIM： To investigate the relationship between donor liver cold preservation, lung surfactant （LS） changes and acute lung injury （ALI） after liver transplantation. METHODS： Liver transplantation models were estab- lished using male Wistar rats. Donor livers were pre- served in University of Wisconsin solution at 4 ℃ for different lengths of time. The effect of ammonium pyr- rolidinedithiocarbamate （PDTC） on ALI was also detect- ed. All samples were harvested after 3 h reperfusion.The severity of AU was evaluated by lung weight/body weight ratio, lung histopathological score, serum nitric oxide （NO） and endothelin （ET）-I levels, lung tumor necrosis factor （TNF）-α and interleukin （IL）-1β levels. Lung surfactants （LSs） were determined by micellar electrokinetic capillary chromatography. RESULTS： With extended donor liver cold preservation time （CPT）, lung histopathological scores, serum ET-i levels, lung weight/body weight ratio and the level of TNF-α and IL-1β in lung were increased significantly in the 180-min group compared with the sham group （3.16± 0.28 vs 1.12 ± 0.21, P 〈 0.001; 343.59±53.97 vs 141.53± 48.48, P 〈 0.001; 0.00687 ±0.00037 vs 0.00557 ±0.00056, P 〈 0.001; 17.5 ± 3.0 vs 1,3 ± 0.3, P 〈 0.001; 10.8± 2.3 vs 1.8 ± 0.4, P 〈 0.001）, but serum NO levels decreased remarkably （74.67 ± 10.01 vs 24.97 ± 3.18, P 〈 0.001）. The expression of lung phos- phatidylcholine （PC）, phosphatidylethanolamine （PE）, phosphatidylinositol （PI） and phosphatidylserine （PS） increased when CPT was 〈 120 rain, and decreased when CPT was 〉 180 min （PC： 1318.89 ± 54.79 vs 1011.18± 59.99, P 〈 0.001; PE： 1504.45± 119.96 vs 1340.80±76.39, P = 0.0019; PI： 201.23 ± 34.82 vs 185.88 ± 17.04, P = 0.2265; PS： 300.43±32.95 vs 286.55± 55.55, P = 0.5054）. All these ALI-associated indexes could be partially reversed by PDTC treatment.
文摘To observe the changes of evoked potentials after severe brain injury and the effect of mild hypothermia on acute severe brain injury. Methods: A total of 44 patients with severe closed head injury (GCS 3 8, admitted within 10 hours from injury) admitted from May 1998 to March 1999 were selected for this study. All patients were admitted into the intensive care unit and divided into 2 groups, Group A (GCS 3 5) and Group B (GCS 6 8). Patients were also randomly assigned to either normothermia or hypothermia subgroups. Patients in the hypothermia group were cooled to 32 34℃. Median nerve short latency somatosensory evoked potentials (SLSEP) and brain stem auditory evoked potentials (BAEP) were recorded before cooling and 4, 24, 48, 72, 96 and 120 hours, respectively after cooling and temperature resuming. SLSEP and BAEP were measured at the same time in the normothermia group (control group). The changes of evoked potentials (EP) were analyzed by statistical methods. Results: In the Group B, N 20 amplitudes in SLSEP and I/V amplitudes in BAEP after mild hypothermia treatment in the hypothermia group differed significantly from those in the control group (P< 0.05 ). However, in the Group A, no significant difference in all parameters was found. Conclusions: These results demonstrate that mild hypothermia treatment (32 34℃) in the Group B has a significant neuroelectrophysiological effect on severe brain injury. Nevertheless, the effect of mild hypothermia in the Group A is not apparent and needs further studying.
