To characterize acute-phase hepatitis B virus (HBV)-specific T cell responses associated with self-limited and persistent HBV infections, we compared a patient with acute HBV/HCV coinfection, who was able to control H...To characterize acute-phase hepatitis B virus (HBV)-specific T cell responses associated with self-limited and persistent HBV infections, we compared a patient with acute HBV/HCV coinfection, who was able to control HCV but developed chronic hepatitis B, with patients who resolved acute HBV infection spontaneously. Acute-phase CD4 responses were efficient in self-limited infections but undetectable in the coinfected patient with HBV persistence. CD8 responses were multispecific irrespective of the outcome of infection, but the CD8 repertoire associated with HBV persistence lacked the most dominant specificities detectable in self-limited infections. In conclusion, insufficient CD4 help and defective CD8 repertoire may play a role at the early stages of infection in influencing HBV persistence.展开更多
Background/Aims:Characteristics of the intrahepatic virus-specific T-cell response in patients with acute hepatitis B virus(HBV) infection have not been studied due to the risk of complications associated with standar...Background/Aims:Characteristics of the intrahepatic virus-specific T-cell response in patients with acute hepatitis B virus(HBV) infection have not been studied due to the risk of complications associated with standard liver biopsies.In this study we aimed to characterize the virus-specific CD8 + T-cell response in the liver of patients with acute HBV infection using fine-needle aspiration-biopsy(FNAB) .Methods:In HLA-A2 positive patients with acute HBV infection a FNAB was performed at first presentation,at the time of HBsAg-seroconversion and 3 months after HBsAg-seroconversion.HLA-A2 tetramers were used to identify HBV-specific CD8 + T-cells in FNAB-cytology and peripheral blood(PB) .Results:At first presentation there was a correlation between the frequency of intrahepatic CD8 + T-cells and the degree of liver damage.At all time points there was sequestering of HBV-specific CD8 + T-cells in the liver,and the percentage of intrahepatic HLA-DR expressing HBV-specific CD8 + T-cells was higher than in PB.Three months after HBsAg-seroconversion the frequency of intrahepatic HBV-specific CD8 + T-cells remained high.Conclusions:HBV-specific CD8 + T-cells are compartmentalized in the liver during acute HBV infection.Their presence in the liver may suggest a role in the resolution of the infection.Intrahepatic HBV-specific CD8 + T cells remain detectable at high frequencies after HBsAg-seroconversion.展开更多
文摘To characterize acute-phase hepatitis B virus (HBV)-specific T cell responses associated with self-limited and persistent HBV infections, we compared a patient with acute HBV/HCV coinfection, who was able to control HCV but developed chronic hepatitis B, with patients who resolved acute HBV infection spontaneously. Acute-phase CD4 responses were efficient in self-limited infections but undetectable in the coinfected patient with HBV persistence. CD8 responses were multispecific irrespective of the outcome of infection, but the CD8 repertoire associated with HBV persistence lacked the most dominant specificities detectable in self-limited infections. In conclusion, insufficient CD4 help and defective CD8 repertoire may play a role at the early stages of infection in influencing HBV persistence.
文摘Background/Aims:Characteristics of the intrahepatic virus-specific T-cell response in patients with acute hepatitis B virus(HBV) infection have not been studied due to the risk of complications associated with standard liver biopsies.In this study we aimed to characterize the virus-specific CD8 + T-cell response in the liver of patients with acute HBV infection using fine-needle aspiration-biopsy(FNAB) .Methods:In HLA-A2 positive patients with acute HBV infection a FNAB was performed at first presentation,at the time of HBsAg-seroconversion and 3 months after HBsAg-seroconversion.HLA-A2 tetramers were used to identify HBV-specific CD8 + T-cells in FNAB-cytology and peripheral blood(PB) .Results:At first presentation there was a correlation between the frequency of intrahepatic CD8 + T-cells and the degree of liver damage.At all time points there was sequestering of HBV-specific CD8 + T-cells in the liver,and the percentage of intrahepatic HLA-DR expressing HBV-specific CD8 + T-cells was higher than in PB.Three months after HBsAg-seroconversion the frequency of intrahepatic HBV-specific CD8 + T-cells remained high.Conclusions:HBV-specific CD8 + T-cells are compartmentalized in the liver during acute HBV infection.Their presence in the liver may suggest a role in the resolution of the infection.Intrahepatic HBV-specific CD8 + T cells remain detectable at high frequencies after HBsAg-seroconversion.
