[Objective] This study aimed to investigate the acute oral toxicity of crude monkshood （Radix aconiti lateralis preparata）. [Method] Monkshood alcohol extract was prepared using soxhlet extractor. The maximum drug d...[Objective] This study aimed to investigate the acute oral toxicity of crude monkshood （Radix aconiti lateralis preparata）. [Method] Monkshood alcohol extract was prepared using soxhlet extractor. The maximum drug dose, which was the minimum dose causing 100% lethally rate in the preliminary test, was diluted into seven con- centrations in formal test. Then every mouse was orally administrated with 0.04 ml/g of the monkshood solution at single time for 7 d. Median lethal dose （LD50） and 95% confidence limit were calculated by the improved Karber method formulas. [Result] LD50 of monkshood ethanol extract was 230.12 mg/kg and 95% confidence limit of LD50 was 80.39-658.57 mg/kg. [Conclusion] Crude monkshood alcohol extract can quickly induce acute toxicity in mice.展开更多
[Objective] This study was conducted to determine the safe dose of Tongmai Tangyanming capsule in clinic by evaluating its acute toxicity, so as to ensure clinical medication safety. [Method] The test was designed acc...[Objective] This study was conducted to determine the safe dose of Tongmai Tangyanming capsule in clinic by evaluating its acute toxicity, so as to ensure clinical medication safety. [Method] The test was designed according to ＂Technique Requirements of Research of New Chinese Medicine＂ and ＂Guide for Research of New Chinese Medicine＂, mice were intragastrically administrated with Tongmai Tangyanming capsule at a maximum concentration and a maximum volume, and the acute toxic response of mice was observed, so as to determine medi- na lethal dose （LDso） and maximum administration dosage of Tongmai Tangyanming capsule. [Result] The LD^o could not be detected in the test, and the maximum administration dosage of mouse was calculated to be 112.104 g of dried herbs/（kg.d）, equivalent to 260 times of clinical dose of adult. [Conclusion] Chinese herbal compound Tongmai Tangyanming capsule has low acute toxicity, and its clinical dose is safe and reliable.展开更多
采用静水式试验方法,在水温为20±1℃的条件下,研究夹竹桃提取物对鲤鱼、鳊鱼和白鲢的毒性效应,以期为该药物在渔病防治中的使用提供用药的参考依据。试验结果表明,夹竹桃提取物对鲤鱼的24 h LC_(50)、48 h LC_(50)、96 h LC_(50)...采用静水式试验方法,在水温为20±1℃的条件下,研究夹竹桃提取物对鲤鱼、鳊鱼和白鲢的毒性效应,以期为该药物在渔病防治中的使用提供用药的参考依据。试验结果表明,夹竹桃提取物对鲤鱼的24 h LC_(50)、48 h LC_(50)、96 h LC_(50)分别为149．55 mg/L、78．76 mg/L、66．09 mg/L;安全浓度为6．61 mg/L。对鳊鱼的24 h LC_(50)、48 h LC_(50)、96 h LC_(50)分别为269．41 mg/L、94．54 mg/L、40．63 mg/L;安全浓度为4．06 mg/L。对白鲢的24 h LC^(50)、48 h LC^(50)、96 h LC^(50)分别为91．37 mg/L、69．09 mg/L、48．71 mg/L,安全浓度为4．87 mg/L。展开更多
基金Supported by the National Natural Science Foundation of China (31201951)~~
文摘[Objective] This study aimed to investigate the acute oral toxicity of crude monkshood （Radix aconiti lateralis preparata）. [Method] Monkshood alcohol extract was prepared using soxhlet extractor. The maximum drug dose, which was the minimum dose causing 100% lethally rate in the preliminary test, was diluted into seven con- centrations in formal test. Then every mouse was orally administrated with 0.04 ml/g of the monkshood solution at single time for 7 d. Median lethal dose （LD50） and 95% confidence limit were calculated by the improved Karber method formulas. [Result] LD50 of monkshood ethanol extract was 230.12 mg/kg and 95% confidence limit of LD50 was 80.39-658.57 mg/kg. [Conclusion] Crude monkshood alcohol extract can quickly induce acute toxicity in mice.
基金Supported by Modern Pharmaceutical Program of Guiyang Scientific and Technological Planning Project in 2013(ZKHT[2013204]4-6)Special Fund for Research and Development of Traditional Chinese Medicine(TCM)Modernization Technology Industry(QKHZYZ[2011]5012)~~
文摘[Objective] This study was conducted to determine the safe dose of Tongmai Tangyanming capsule in clinic by evaluating its acute toxicity, so as to ensure clinical medication safety. [Method] The test was designed according to ＂Technique Requirements of Research of New Chinese Medicine＂ and ＂Guide for Research of New Chinese Medicine＂, mice were intragastrically administrated with Tongmai Tangyanming capsule at a maximum concentration and a maximum volume, and the acute toxic response of mice was observed, so as to determine medi- na lethal dose （LDso） and maximum administration dosage of Tongmai Tangyanming capsule. [Result] The LD^o could not be detected in the test, and the maximum administration dosage of mouse was calculated to be 112.104 g of dried herbs/（kg.d）, equivalent to 260 times of clinical dose of adult. [Conclusion] Chinese herbal compound Tongmai Tangyanming capsule has low acute toxicity, and its clinical dose is safe and reliable.
文摘采用静水式试验方法,在水温为20±1℃的条件下,研究夹竹桃提取物对鲤鱼、鳊鱼和白鲢的毒性效应,以期为该药物在渔病防治中的使用提供用药的参考依据。试验结果表明,夹竹桃提取物对鲤鱼的24 h LC_(50)、48 h LC_(50)、96 h LC_(50)分别为149．55 mg/L、78．76 mg/L、66．09 mg/L;安全浓度为6．61 mg/L。对鳊鱼的24 h LC_(50)、48 h LC_(50)、96 h LC_(50)分别为269．41 mg/L、94．54 mg/L、40．63 mg/L;安全浓度为4．06 mg/L。对白鲢的24 h LC^(50)、48 h LC^(50)、96 h LC^(50)分别为91．37 mg/L、69．09 mg/L、48．71 mg/L,安全浓度为4．87 mg/L。
