目的探讨新辅助化疗对宫颈癌患者急性血液学毒性和免疫抑制的影响。方法选择2016年9月至2018年9月西安交通大学第一附属医院收治的82例宫颈癌患者为研究对象,依据随机数字表法将入选患者分为观察组和对照组,每组各41例。对照组患者接受...目的探讨新辅助化疗对宫颈癌患者急性血液学毒性和免疫抑制的影响。方法选择2016年9月至2018年9月西安交通大学第一附属医院收治的82例宫颈癌患者为研究对象,依据随机数字表法将入选患者分为观察组和对照组,每组各41例。对照组患者接受常规化疗,观察组患者接受新辅助化疗,均于化疗结束后行肿瘤细胞减灭术。比较两组患者近期疗效、化疗前后生存质量、化疗前后髓源性抑制细胞(myeloid-derived suppressor cell,MDSC)和调节性T细胞(regulatory T cell,Treg)水平、化疗期间急性血液学毒性反应发生情况。结果观察组患者治疗总有效率显著高于对照组(P<0.05)。两组患者化疗后世界卫生组织生存质量测定量表简表(World Health Organization quality of life-brief version,WHOQOL-BREF)评分、MDSC和Treg水平均显著高于本组化疗前(均P<0.05);观察组患者化疗后WHOQOL-BREF评分显著高于对照组(P<0.05),MDSC和Treg水平均显著低于对照组(均P<0.05)。两组患者化疗期间急性血液学毒性反应发生率比较差异均无统计学意义(均P>0.05)。结论新辅助化疗对宫颈癌患者近期疗效良好,对免疫功能影响小,且急性血液学毒性反应发生率较低。展开更多
The acute （15 minutes-4 hours） effects of exposure to the inhaled DEE （diesel exhaust emitted） on blood parameters of Guinea pigs Cavia porcellus were assessed via CBC （complete blood count） as an indicator usin...The acute （15 minutes-4 hours） effects of exposure to the inhaled DEE （diesel exhaust emitted） on blood parameters of Guinea pigs Cavia porcellus were assessed via CBC （complete blood count） as an indicator using an automated blood analyzer machines （Cell DYN 1700）. The results showed significant changes in some parameters in comparison with control animals started as early as 15 minutes and intensified by the continuous exposure time, leading to significant increase （p 〈 0.05） in the number of both WBC （white blood cells）, PLT （blood platelets） and decrease in numbers of RBC （red blood corpuscles） while hemoglobin percentages （Hb%） showed an insignificant （p 〉 0.05） fluctuating rhythm. The percentages of both Neutrophil and Lymphocyt recorded a significant increase （p 〈 0.05） 1 hour post-exposure to the DEE while other WBCs showed less changes. It is concluded that blood components are so sensitive to DEE and do respond quicker to inhaled pollutants via changes in some components of WBC% but decrease in RBC% and the likelihood of blood clotting due to increase in blood platelets. These results indicate the acute toxic risks of the exposure to DEE of mechanics and technicians who work in vicinity of DEE sources or/and spend most daily hours in semi closed areas.展开更多
OBJECTIVE: To examine the acute toxicity of an aqueous extract of Aspidopterys obcordata(A. obcordata) in Sprague Dawley rats.METHODS: The rats were orally administered a dose of 5000 mg/kg body weight and observed co...OBJECTIVE: To examine the acute toxicity of an aqueous extract of Aspidopterys obcordata(A. obcordata) in Sprague Dawley rats.METHODS: The rats were orally administered a dose of 5000 mg/kg body weight and observed continuously for 6 h and then daily for 14 days. Control rats were administered distilled water. The effect of the extract on general behavior, body weight, and food and water intake were measured.After 14 days, the rats were sacrificed and their organs(liver, heart, spleen, lungs, kidney, adrenal glands, ovaries, and testes) were removed for macroscopic examination. The body and organ weights in addition to hematology(e.g., hemoglobin and white blood cell counts) and clinical blood biochemistry(e.g., albumin and bilirubin) were also examined.RESULTS: There were no deaths recorded, and the rats treated with A. obcordata showed no signs of toxicity. All measured parameters in rats treated with A. obcordata were unaffected when compared with those in control rats. The acute toxicity(LD_(50))was estimated to be > 5000 mg/kg body weight.CONCLUSION: Our results demonstrate the safety of an acute oral administration of an aqueous extract of A. obcordata in rats and indicate that future subacute and long-term toxicity testing of A. obcordata is warranted.展开更多
文摘目的探讨新辅助化疗对宫颈癌患者急性血液学毒性和免疫抑制的影响。方法选择2016年9月至2018年9月西安交通大学第一附属医院收治的82例宫颈癌患者为研究对象,依据随机数字表法将入选患者分为观察组和对照组,每组各41例。对照组患者接受常规化疗,观察组患者接受新辅助化疗,均于化疗结束后行肿瘤细胞减灭术。比较两组患者近期疗效、化疗前后生存质量、化疗前后髓源性抑制细胞(myeloid-derived suppressor cell,MDSC)和调节性T细胞(regulatory T cell,Treg)水平、化疗期间急性血液学毒性反应发生情况。结果观察组患者治疗总有效率显著高于对照组(P<0.05)。两组患者化疗后世界卫生组织生存质量测定量表简表(World Health Organization quality of life-brief version,WHOQOL-BREF)评分、MDSC和Treg水平均显著高于本组化疗前(均P<0.05);观察组患者化疗后WHOQOL-BREF评分显著高于对照组(P<0.05),MDSC和Treg水平均显著低于对照组(均P<0.05)。两组患者化疗期间急性血液学毒性反应发生率比较差异均无统计学意义(均P>0.05)。结论新辅助化疗对宫颈癌患者近期疗效良好,对免疫功能影响小,且急性血液学毒性反应发生率较低。
文摘The acute （15 minutes-4 hours） effects of exposure to the inhaled DEE （diesel exhaust emitted） on blood parameters of Guinea pigs Cavia porcellus were assessed via CBC （complete blood count） as an indicator using an automated blood analyzer machines （Cell DYN 1700）. The results showed significant changes in some parameters in comparison with control animals started as early as 15 minutes and intensified by the continuous exposure time, leading to significant increase （p 〈 0.05） in the number of both WBC （white blood cells）, PLT （blood platelets） and decrease in numbers of RBC （red blood corpuscles） while hemoglobin percentages （Hb%） showed an insignificant （p 〉 0.05） fluctuating rhythm. The percentages of both Neutrophil and Lymphocyt recorded a significant increase （p 〈 0.05） 1 hour post-exposure to the DEE while other WBCs showed less changes. It is concluded that blood components are so sensitive to DEE and do respond quicker to inhaled pollutants via changes in some components of WBC% but decrease in RBC% and the likelihood of blood clotting due to increase in blood platelets. These results indicate the acute toxic risks of the exposure to DEE of mechanics and technicians who work in vicinity of DEE sources or/and spend most daily hours in semi closed areas.
基金National Natural Science Foundation of China(Transport Characters and Mechanism Research of Multiplicity Ingredient TCM Utilizing Sensitive Bioactivity in Caco-2 Cell Model,No.81173645)Program for Innovative Research Team in IMPLAD(Discovery and foundation of new drug of TCM)+2 种基金a grant from the Basic Scientific Research Special of the Central Public Welfare Research Institutes of IMPLAD(Research on the Kidney Stone Activities and Preliminary Mechanisms of Aspidopterys Obcordata,No.YZYN-15-06)PUMC Youth Fund(Study of Anti-Tumor Activities of Pegylation Artemisinin Prodrugs Based on PK-PD Binding Model,No.33320140076)Beijing Municipal Natural Science Foundation(Preparation of Silica-Based Nanoparticles/PDMS Hybrid Membranes for Pervaporation of Ethanol/Water Mixtures,No.2132010)
文摘OBJECTIVE: To examine the acute toxicity of an aqueous extract of Aspidopterys obcordata(A. obcordata) in Sprague Dawley rats.METHODS: The rats were orally administered a dose of 5000 mg/kg body weight and observed continuously for 6 h and then daily for 14 days. Control rats were administered distilled water. The effect of the extract on general behavior, body weight, and food and water intake were measured.After 14 days, the rats were sacrificed and their organs(liver, heart, spleen, lungs, kidney, adrenal glands, ovaries, and testes) were removed for macroscopic examination. The body and organ weights in addition to hematology(e.g., hemoglobin and white blood cell counts) and clinical blood biochemistry(e.g., albumin and bilirubin) were also examined.RESULTS: There were no deaths recorded, and the rats treated with A. obcordata showed no signs of toxicity. All measured parameters in rats treated with A. obcordata were unaffected when compared with those in control rats. The acute toxicity(LD_(50))was estimated to be > 5000 mg/kg body weight.CONCLUSION: Our results demonstrate the safety of an acute oral administration of an aqueous extract of A. obcordata in rats and indicate that future subacute and long-term toxicity testing of A. obcordata is warranted.
