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乙烯利种子期雌性化处理后黄瓜性别信号蛋白质的研究——过氧化物同工酶酶谱分析
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作者 孙晶洁 《农家致富顾问》 2020年第6期162-162,共1页
在黄瓜种子期,分别用浓度为1000、2000、3000、4000、5000和6000mg/L(以蒸馏水为对照)的乙烯利进行雌性化处理后,对其体内的过氧化物同工酶从子叶期至第五真叶期进行聚丙烯酰胺凝胶电泳分离分析.结果表明,黄瓜种子期不同浓度乙烯利雌性... 在黄瓜种子期,分别用浓度为1000、2000、3000、4000、5000和6000mg/L(以蒸馏水为对照)的乙烯利进行雌性化处理后,对其体内的过氧化物同工酶从子叶期至第五真叶期进行聚丙烯酰胺凝胶电泳分离分析.结果表明,黄瓜种子期不同浓度乙烯利雌性化处理后,处理和对照的过氧化物酶同工酶酶谱无显著差异. 展开更多
关键词 黄瓜 乙烯利 种子期雌性化处理 性别信号蛋白质 过氧化物同工酶
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基于SIRT1/SOX2信号通路探究西黄丸抗乳腺癌作用及机制 被引量:1
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作者 吴海滨 马志强 +4 位作者 李帅 张冠男 苗雅云 湛喜梅 王文胜 《天津中医药》 CAS 2023年第6期775-781,共7页
[目的]基于沉默调节蛋白1(SIRT1)/性别决定区Y框蛋白2(SOX2)信号通路探究西黄丸抗乳腺癌作用及机制。[方法]将MDA-MB-231细胞分为对照组、西黄丸低剂量组(5 g/L)、西黄丸中剂量组(7.5 g/L)、西黄丸高剂量组(15 g/L)、SIRT1激活剂(SRT 17... [目的]基于沉默调节蛋白1(SIRT1)/性别决定区Y框蛋白2(SOX2)信号通路探究西黄丸抗乳腺癌作用及机制。[方法]将MDA-MB-231细胞分为对照组、西黄丸低剂量组(5 g/L)、西黄丸中剂量组(7.5 g/L)、西黄丸高剂量组(15 g/L)、SIRT1激活剂(SRT 1720)组(6μmol/L)、西黄丸高剂量+SRT 1720组(15 g/L+6μmol/L);CCK-8法和平板克隆实验检测细胞增殖;划痕实验检测细胞迁移;Transwell法检测细胞侵袭;蛋白免疫印迹法(Western Blot)检测细胞中增殖细胞核抗原(PCNA)、基质金属蛋白酶(MMP)-2、MMP-9、SIRT1、SOX2蛋白表达;体内移植瘤实验检测各组裸鼠肿瘤体积、质量及PCNA、MMP-2、MMP-9、SIRT1、SOX2蛋白表达。[结果]西黄丸可抑制MDA-MB-231细胞增殖、迁移、侵袭及裸鼠体内移植瘤的生长,且呈剂量依赖性;SIRT1激活剂SRT 1720促进MDA-MB-231细胞增殖、迁移、侵袭及裸鼠体内移植瘤的生长;高剂量西黄丸加SIRT1激活剂组可以减弱高剂量西黄丸对MDA-MB-231细胞增殖、迁移、侵袭及裸鼠体内移植瘤的生长的抑制作用;同时西黄丸可呈剂量依赖性地抑制MDA-MB-231细胞及裸鼠移植瘤中SIRT1、SOX2蛋白表达,抑制SIRT1/SOX2信号通路活性,并通过降低PCNA、MMP-2、MMP-9表达来抑制MDA-MB-231细胞增殖、迁移、侵袭及裸鼠体内移植瘤的生长。[结论]西黄丸可能通过抑制SIRT1/SOX2信号通路抑制MDA-MB-231细胞增殖、迁移、侵袭及裸鼠体内移植瘤生长。 展开更多
关键词 西黄丸 沉默调节蛋白1/性别决定区Y框蛋白2信号通路 乳腺癌 增殖 迁移
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黄芩总黄酮对人骨关节炎软骨细胞增殖凋亡及COL10A1/SOX9信号通路的影响 被引量:4
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作者 王冠贤 李宝林 +1 位作者 唐爱民 黄永湘 《中国药师》 CAS 2021年第4期693-697,共5页
目的:探讨黄芩总黄酮对人骨关节炎(OA)软骨细胞增殖凋亡及X型胶原蛋白α1链(COL10A1)/性别决定区Y框蛋白9(SOX9)信号通路的影响。方法:从5例行膝关节置换术的OA患者的关节软骨分离软骨细胞,用甲苯胺蓝染色进行鉴定。实验分为对照组、黄... 目的:探讨黄芩总黄酮对人骨关节炎(OA)软骨细胞增殖凋亡及X型胶原蛋白α1链(COL10A1)/性别决定区Y框蛋白9(SOX9)信号通路的影响。方法:从5例行膝关节置换术的OA患者的关节软骨分离软骨细胞,用甲苯胺蓝染色进行鉴定。实验分为对照组、黄芩总黄酮低、中、高剂量组(25,50,100 mg·L-1)和塞来昔布组(9 mg·L-1)。噻唑蓝(MTT)法测定人OA软骨细胞增殖情况,流式细胞仪测定细胞凋亡水平,免疫印迹法检测人OA软骨细胞中COL10A1、SOX9、细胞外信号调节激酶1/2(ERK1/2)、B细胞淋巴瘤/白血病-2(Bcl-2)和Bcl-2相关X蛋白(Bax)蛋白水平。结果:人OA软骨细胞附着于培养皿后呈多边形或长条形,甲苯胺蓝染色可见OA软骨细胞胞浆以蓝紫色为主,异染颗粒散布于细胞间或细胞周围;与对照组比较,黄芩总黄酮低、中、高剂量组和塞来昔布组人OA软骨细胞增殖率、COL10A1、SOX9、Bcl-2蛋白和Bcl-2/Bax水平升高,凋亡率、ERK1/2和Bax蛋白水平降低(P<0.05);随着黄芩总黄酮浓度的增加,黄芩总黄酮各剂量组人OA软骨细胞增殖率、COL10A1、SOX9、Bcl-2蛋白和Bcl-2/Bax水平逐渐升高,凋亡率、ERK1/2和Bax蛋白水平逐渐降低(P<0.05);黄芩总黄酮高剂量组与塞来昔布组作用相似,上述指标差异无统计学意义(P>0.05)。结论:黄芩总黄酮可能通过激活COL10A1/SOX9信号通路,抑制人OA软骨细胞凋亡,促进OA软骨细胞增殖。 展开更多
关键词 黄芩总黄酮 骨关节炎 细胞增殖 细胞凋亡 X型胶原蛋白α1链/性别决定区Y框蛋白9信号通路
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Analysis on pulse features of coronary heart disease patients with or without a history of ischemic stroke
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作者 LI Xin LI Wei +4 位作者 NG Man-In PARRY Natalie Ann LI Siqi LI Rui GUO Rui 《Digital Chinese Medicine》 CAS CSCD 2024年第3期264-273,共10页
Objective To evaluate the capability of wrist pulse analysis in distinguishing three physiolog-ical and pathological conditions:healthy individuals,coronary heart disease(CHD)patients without a history of ischemic str... Objective To evaluate the capability of wrist pulse analysis in distinguishing three physiolog-ical and pathological conditions:healthy individuals,coronary heart disease(CHD)patients without a history of ischemic stroke,and CHD patients with a history of ischemic stroke.Methods Study participants were recruited from Shuguang East Hospital,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,and Shanghai Municipal Hospital of Traditional Chinese Medicine,affiliated with Shanghai University of Traditional Chinese Medicine,from April 15 to September 15,2021.They were categorized into three groups:healthy controls(Group 1),CHD patients without a history of ischemic stroke(Group 2),and CHD patients with a history of ischemic stroke(Group 3).The wrist pulse signals of the study participants were non-invasively collected using a pulse diagnosis instrument.The linear time-domain features and nonlinear time-series multiscale entropy(MSE)features of the pulse signals were extracted using time-domain analysis and the MSE methods,which were subsequently compared between groups.Based on these extracted features,a recognition model was developed using a random forest(RF)algorithm.The classification performance of the models was evaluated using metrics,including accuracy,precision,recall,and F1-score derived from confusion matrix as well as the area under the receiver operating characteristics(ROC)curve(AUC).Results A total of 189 participants were enrolled,with 63 in Group 1,61 in Group 2,and 65 in Group 3.Compared with Group 1,Group 2 showed significant increases in pulse features H2/H1,H3/H1,W1,W2,and W2/T,and decreased in MSE_(1)-MSE7(P<0.05),while Group 3 showed significant increases in pulse features T5/T4,T,H1/T1,W1,W2,AS,and Ad,and de-creased in MSE_(1)-MSE_(20)(P<0.05).Compared with Group 2,Group 3 demonstrated notable increases in H1/T1 and As(P<0.05).The RF model achieved precision of 80.00%,61.54%,and 61.54%,recall of 74.29%,60.00%,and 68.97%,F1-scores of 70.04%,60.76%,and 65.04%,and AUC values of 0.92,0.74,and 0.81 for Groups 1,2,and 3,respectively.The overall accuracy was 67.69%,with micro-average AUC of 0.83 and macro-average AUC of 0.82.Conclusion Differences in pulse features reflect variations in arterial compliance,peripheral resistance,cardiac afterload,and pulse signal complexity among healthy individuals,CHD patients without a history of ischemic stroke,and those with such a history.The developed pulse-based recognition model holds the potential in distinguishing between these three groups,offering a novel diagnostic reference for clinical practice. 展开更多
关键词 Pulse diagnosis Coronary heart disease(CHD) Ischemic stroke Signal processing Pattern recognition
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And Gate Recognition System for Short Range Targets 被引量:1
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作者 王军波 周忠来 施聚生 《Journal of Beijing Institute of Technology》 EI CAS 1997年第4期54-60,共7页
Being aimed at the weakness of short range target′s threshold value recognition system,the double passage And Gate recognition system was put forward on the correlativity of target signals and randomness of noise ... Being aimed at the weakness of short range target′s threshold value recognition system,the double passage And Gate recognition system was put forward on the correlativity of target signals and randomness of noise signals Through state analysis and inference of state transition probability,both the reliability and early burst probability of the system were obtained in theory 展开更多
关键词 signal recognition RELIABILITY target detector
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爱美与进化
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作者 黄斌 《大自然探索》 2002年第11期55-56,共2页
有人说,在人类“爱情不可言喻的神秘与伟大之下,还隐藏着一些基本的生物学法则和基因法则”。
关键词 生物进化 色彩 性别信号 交配
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比翼双飞的背后
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作者 马特 《浙江林业》 2000年第6期28-28,共1页
一只雌性家燕在空中飞来飞去,寻找配偶。她很挑剔,决不想随便凑合。突然,一抹亮色在她眼前闪过:是一位雄武的追求者。她马上就被他吸引住了。他那鲜红的喉囊太迷人了,她实在无法抗拒。
关键词 动物 性别选择 性别信号 类胡萝卜素 免疫功能
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Cluster analysis of the domain of microseismic event attributes for fl oor water inrush warning in the working face
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作者 Shang Guo-Jun Liu Xiao-Fei +3 位作者 Li Li Zhao Li-Song Shen Jin-Song Huang Wei-Lin 《Applied Geophysics》 SCIE CSCD 2022年第3期409-423,471,472,共17页
Differences are found in the attributes of microseismic events caused by coal seam rupture,underground structure activation,and groundwater movement in coal mine production.Based on these differences,accurate classific... Differences are found in the attributes of microseismic events caused by coal seam rupture,underground structure activation,and groundwater movement in coal mine production.Based on these differences,accurate classification and analysis of microseismic events are important for the water inrush warning of the coal mine working facefloor.Cluster analysis,which classifies samples according to data similarity,has remarkable advantages in nonlinear classification.A water inrush early warning method for coal minefloors is proposed in this paper.First,the short time average over long time average(STA/LTA)method is used to identify effective events from continuous microseismic records to realize the identification of microseismic events in coal mines.Then,ten attributes of microseismic events are extracted,and cluster analysis is conducted in the attribute domain to realize unsupervised classification of microseismic events.Clustering results of synthetic andfield data demonstrate the effectiveness of the proposed method.The analysis offield data clustering results shows that thefirst kind of events with time change rules is of considerable importance to the early warning of water inrush from the coal mine working facefloor. 展开更多
关键词 signal detection attribute extraction cluster analysis and water disaster warning
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Practical Identity-Based Aggregate Signature from Bilinear Maps 被引量:1
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作者 王竹 吴倩 +1 位作者 叶顶锋 陈辉焱 《Journal of Shanghai Jiaotong university(Science)》 EI 2008年第6期684-687,共4页
A new identity-based (ID-based) aggregate signature scheme which does not need any kind of interaction among the signers was proposed to provide partial aggregation. Compared with the existing ID-based aggregate sig... A new identity-based (ID-based) aggregate signature scheme which does not need any kind of interaction among the signers was proposed to provide partial aggregation. Compared with the existing ID-based aggregate signatures, the scheme is more efficient in terms of computational cost, Security in the random oracle model based on a variant of the computation Diflle-Hellman (CDH) problem is captured. 展开更多
关键词 identity-based signature bilinear map aggregate signature: batch verification
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Adult meerkats modify close call rate in the presence of pups 被引量:1
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作者 Megan T. WYMAN Pearl R. RIVERS +2 位作者 Coline MULLER Pauline TONI Marta B. MANSER 《Current Zoology》 SCIE CAS CSCD 2017年第3期349-355,共7页
In animals, signaling behavior is often context-dependent, with variation in the probability of emitting certain signals dependent on fitness advantages. Senders may adjust signaling rate depending on receiver identit... In animals, signaling behavior is often context-dependent, with variation in the probability of emitting certain signals dependent on fitness advantages. Senders may adjust signaling rate depending on receiver identity, presence of audiences, or noise masking the signal, all of which can affect the benefits and costs of signal production. In the cooperative breeding meerkat Suricata suricatta, group members emit soft contact calls, termed as "close calls", while foraging in order to maintain group cohesion. Here, we investigated how the close calling rate during foraging was affected by the presence of pups, that produce continuous, noisy begging calls as they follow older group members. Adults decreased their overall close call rate substantially when pups were foraging with the group in comparison to periods when no pups were present. We suggest this decrease was likely due to a masking effect of the loud begging calls, which makes the close call function of maintaining group cohesion partly redundant as the centrally located begging calls can be used in- stead to maintain cohesion. There was some support that adults use close calls strategically to at- tract specific pups based on fitness advantages, that is, as the philopatric sex, females should call more than males and more to female pups than male pups. Dominant females called more than dominant males when a pup was in close proximity, while subordinates showed no sex-based dif- ferences. The sex of the nearest pup did not affect the calling rate of adults. The study shows that meerkats modify their close call production depending on benefits gained from calling and provides an example of the flexible use of one calling system in the presence of another, here contact calls versus begging calls, within the same species. 展开更多
关键词 call rate communication contact calls cooperation flexibility group cohesion.
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