探讨抗性/易感小鼠DNA识别受体的组织表达差异与抗病性的关系。方法不同抗性品系小鼠感染ECTV后,采用实时荧光定量PCR(qRT-PCR)技术分析其组织的重要DNA识别受体差异表达谱。结果TLR9、DAI、cGAS、RNA pol III和RIG-I这5个与DNA识别相...探讨抗性/易感小鼠DNA识别受体的组织表达差异与抗病性的关系。方法不同抗性品系小鼠感染ECTV后,采用实时荧光定量PCR(qRT-PCR)技术分析其组织的重要DNA识别受体差异表达谱。结果TLR9、DAI、cGAS、RNA pol III和RIG-I这5个与DNA识别相关的受体在不同抗性小鼠组织中均呈广泛性表达,但鼠痘抗性小鼠C57BL/6品系的表达明显整体高于BALB/c小鼠;在ECTV感染后易感小鼠BALB/c品系的这些基因表达明显上调,而C57BL/6小鼠变化不大甚至下调。结论这种差异表达特征表明这些受体分子可能与小鼠的天然抗病力密切相关。展开更多
[Objective] This study aimed to avoid and delay the generation of resis- tance in Botrytis cinerea to boscalid. [Method] The sensitivity of Botrytis cinerea to boscalid was tested by the mycelial growth rate method. T...[Objective] This study aimed to avoid and delay the generation of resis- tance in Botrytis cinerea to boscalid. [Method] The sensitivity of Botrytis cinerea to boscalid was tested by the mycelial growth rate method. The distribution of sensitivity variation of B. cinerea to boscalid in different regions was cleared, and the sen- sitivity baseline of B. cinerea from Liaoning Province to boscalid was established. [Result] The ECso values of B. cinerea strains from Liaoning Province ranged from 0.080 0 to 7.787 2μg/ml, and the highest ECho value was 97.34 times higher than the minimum. The average EC50 value （1.973μg/ml） of the 158 strains was treated as the baseline sensitivity of B. cinerea from Liaoning Province to boscalid. [Conclu- sion] It is essential to conduct a risk assessment of drug resistance in B. cinerea to boscalid.展开更多
[Objective] This study aimed to investigate the hereditary stability of sFat-1 transgenic pigs and the differences in disease susceptivity between sFat-1 transgenic pigs and non-transgenic pigs. [Method] The integrati...[Objective] This study aimed to investigate the hereditary stability of sFat-1 transgenic pigs and the differences in disease susceptivity between sFat-1 transgenic pigs and non-transgenic pigs. [Method] The integration of sFat-1 gene in pigs was detected by PCR; the infection of transgenic pig to pseudorabies, leptospirosis, swine dysentery, brucellosis, Mycobacterium tuberculosis, rotavirus and mycoplasma hyopneumoniae was detected by using ELISA and PCR. [Result] The positive ratio of F3 generation sFat-1 transgenic pigs was 18.5%; the susceptivity of positive sFat- 1 transgenic and negative pigs to seven infectious diseases showed no significant difference. [Conclusion] Exogenous gene in sFat-1 transgenic pigs can not be stably inherited. The overall physical condition of positive transgenic and negative pigs was similar.展开更多
In order to improve the accuracy of travel demand forecast and considering the distribution of travel behaviors within time dimension, a trip chaining pattern recognition model was established based on activity purpos...In order to improve the accuracy of travel demand forecast and considering the distribution of travel behaviors within time dimension, a trip chaining pattern recognition model was established based on activity purposes by applying three methods： the support vector machine （SVM） model, the radial basis function neural network （RBFNN） model and the multinomial logit （MNL） model. The effect of explanatory factors on trip chaining behaviors and their contribution to model performace were investigated by sensitivity analysis. Results show that the SVM model has a better performance than the RBFNN model and the MNL model due to its higher overall and partial accuracy, indicating its recognition advantage under a smai sample size scenario. It is also proved that the SVM model is capable of estimating the effect of multi-category factors on trip chaining behaviors more accurately. The different contribution of explanatory, factors to trip chaining pattern recognition reflects the importance of refining trip chaining patterns ad exploring factors that are specific to each pattern. It is shown that the SVM technology in travel demand forecast modeling and analysis of explanatory variable effects is practical.展开更多
In order to find out how the climatic characteristics affect people＇s adaptability to thermal environments,experimental studies in a climate chamber are conducted on the effects of transition seasons（from spring to ...In order to find out how the climatic characteristics affect people＇s adaptability to thermal environments,experimental studies in a climate chamber are conducted on the effects of transition seasons（from spring to summer）and the occupants＇ native areas on indoor thermal sensations.Results reveal that people＇s tolerances to cool and warm indoor environments are different in the transition season.When the outdoor temperature is higher,the occupants have a weaker tolerance to a cool indoor environment,but a stronger tolerance to a warm indoor environment.Besides,it is found that the occupants＇ thermal sensations depend on both the climatic characteristics of the season and their native areas.The people from southern China present a greater tolerance to both warm and cool indoor environments than those from northern China.The reason can be explained according to the occupants＇ adaptability to the climatic characteristics and the indoor thermal environments of their native areas in different climate zones.展开更多
Uniaxial compressive experiments of ultrafine-grained Al fabricated by equal channel angular pressing(ECAP) method were performed at wide temperature and strain rate range. The influence of temperature on flow stress,...Uniaxial compressive experiments of ultrafine-grained Al fabricated by equal channel angular pressing(ECAP) method were performed at wide temperature and strain rate range. The influence of temperature on flow stress, strain hardening rate and strain rate sensitivity was investigated experimentally. The results show that both the effect of temperature on flow stress and its strain rate sensitivity of ECAPed Al is much larger than those of the coarse-grained Al. The temperature sensitivity of ultrafine-grained Al is comparatively weaker than that of the coarse-grained Al. Based on the experimental results, the apparent activation volume was estimated at different temperatures and strain rates. The forest dislocation interactions is the dominant thermally activated mechanism for ECAPed Al compressed at quasi-static strain rates, while the viscous drag plays an important role at high strain rates.展开更多
Inflammation has been shown to play an important role in the progression of Alzheimer＇s disease （AD）. Recent epidemical study indicates that the incidence of AD in some populations is substantially influenced by th...Inflammation has been shown to play an important role in the progression of Alzheimer＇s disease （AD）. Recent epidemical study indicates that the incidence of AD in some populations is substantially influenced by the gene polymorphisms of the inflammation mediators. Meanwhile, an ensured risk factor, the ApoE ε4 allele is also reported to directly promote inflammation. Accordingly, it appears that an individual genetic background has partly determined his predisposition for AD by the extent of the inflammation response to the chronic stimulus by β-amyloid peptide （Aβ） deposits and other antigen stressor in the elderly. Hence we present a hypothesis that the inflammation genotypes may contribute to AD susceptibility. This may provide a new orientation both for future identification of individuals at risk and for personalized medication.展开更多
Eight indica ( Oryza sativa L.) environment-sensitive genic male-sterile (EGMS) lines, 2-2S, K1405S, F131S, 2136S, Pei-Ai 64S, 1290S, GD-IS and N17S, were sequentially seeded with 10-15 d interval at three sites, Wuha...Eight indica ( Oryza sativa L.) environment-sensitive genic male-sterile (EGMS) lines, 2-2S, K1405S, F131S, 2136S, Pei-Ai 64S, 1290S, GD-IS and N17S, were sequentially seeded with 10-15 d interval at three sites, Wuhan in 1997, Guiyang in 1997 and Sanya in 1997 and 1998, China. The results of investigations on self-sterilities showed that all of eight EGMS lines had stable sterile periods of longer than 30 d at Wuhan. They can be used for seed production of two-line hybrid rice, but can not reproduce themselves. Their stable sterile periods were shorter than 30 d at Guiyang, they can reproduce themselves and can not be used for hybrid seed production. In Sanya, their stable sterile periods were longer than 150 d, all of eight lines can be used for seed production in summer and autumn and reproduce themselves in winter. The fertility of all eight lines were sensitive to temperature. The sensitive stages, sensitive duration and critical point of temperatures (CPT) of fertility alteration in various lines were different. The sensitive stages of 2-2S and K1405S were from 18 d to 9 d before heading, the sensitive durations were 7-10 d and the CPTs were 23.7-24.5 degreesC. The sensitive stage, sensitive duration and CPT of F131S were from 17 to 5 d before heading, 13 d and 24.3-24.7 degreesC, respectively. The sensitive stage, sensitive duration and CPT of 2136S were from 18 to 12 d before heading, 7 d and 24.6-25.1 degreesC:, respectively. The sensitive stages, sensitive durations of Pei-Ai 64S, 1290S, N17S and GD-1S were from 24 to 13 d before heading and 10-13 d. And their CPTs were 24.6-25.1 degreesC, 25.5-26.2 degreesC, 25.4-26.1 degreesC,, and 24.1-24.7 degreesC, respectively.展开更多
文摘探讨抗性/易感小鼠DNA识别受体的组织表达差异与抗病性的关系。方法不同抗性品系小鼠感染ECTV后,采用实时荧光定量PCR(qRT-PCR)技术分析其组织的重要DNA识别受体差异表达谱。结果TLR9、DAI、cGAS、RNA pol III和RIG-I这5个与DNA识别相关的受体在不同抗性小鼠组织中均呈广泛性表达,但鼠痘抗性小鼠C57BL/6品系的表达明显整体高于BALB/c小鼠;在ECTV感染后易感小鼠BALB/c品系的这些基因表达明显上调,而C57BL/6小鼠变化不大甚至下调。结论这种差异表达特征表明这些受体分子可能与小鼠的天然抗病力密切相关。
基金Supported by Special Fund for Agro-scientific Research in the Public Interest of China(201303025)~~
文摘[Objective] This study aimed to avoid and delay the generation of resis- tance in Botrytis cinerea to boscalid. [Method] The sensitivity of Botrytis cinerea to boscalid was tested by the mycelial growth rate method. The distribution of sensitivity variation of B. cinerea to boscalid in different regions was cleared, and the sen- sitivity baseline of B. cinerea from Liaoning Province to boscalid was established. [Result] The ECso values of B. cinerea strains from Liaoning Province ranged from 0.080 0 to 7.787 2μg/ml, and the highest ECho value was 97.34 times higher than the minimum. The average EC50 value （1.973μg/ml） of the 158 strains was treated as the baseline sensitivity of B. cinerea from Liaoning Province to boscalid. [Conclu- sion] It is essential to conduct a risk assessment of drug resistance in B. cinerea to boscalid.
基金Supported by National Major Program of Genetically Modified Organism for New Species Cultivation of China(2011ZX08011-004)Project from Hubei Agricultural Science and Technology Innovation Center(2011-620-001-003)~~
文摘[Objective] This study aimed to investigate the hereditary stability of sFat-1 transgenic pigs and the differences in disease susceptivity between sFat-1 transgenic pigs and non-transgenic pigs. [Method] The integration of sFat-1 gene in pigs was detected by PCR; the infection of transgenic pig to pseudorabies, leptospirosis, swine dysentery, brucellosis, Mycobacterium tuberculosis, rotavirus and mycoplasma hyopneumoniae was detected by using ELISA and PCR. [Result] The positive ratio of F3 generation sFat-1 transgenic pigs was 18.5%; the susceptivity of positive sFat- 1 transgenic and negative pigs to seven infectious diseases showed no significant difference. [Conclusion] Exogenous gene in sFat-1 transgenic pigs can not be stably inherited. The overall physical condition of positive transgenic and negative pigs was similar.
基金The Fundamental Research Funds for the Central Universities,the Scientific Innovation Research of College Graduates in Jiangsu Province(No.KYLX_0177)
文摘In order to improve the accuracy of travel demand forecast and considering the distribution of travel behaviors within time dimension, a trip chaining pattern recognition model was established based on activity purposes by applying three methods： the support vector machine （SVM） model, the radial basis function neural network （RBFNN） model and the multinomial logit （MNL） model. The effect of explanatory factors on trip chaining behaviors and their contribution to model performace were investigated by sensitivity analysis. Results show that the SVM model has a better performance than the RBFNN model and the MNL model due to its higher overall and partial accuracy, indicating its recognition advantage under a smai sample size scenario. It is also proved that the SVM model is capable of estimating the effect of multi-category factors on trip chaining behaviors more accurately. The different contribution of explanatory, factors to trip chaining pattern recognition reflects the importance of refining trip chaining patterns ad exploring factors that are specific to each pattern. It is shown that the SVM technology in travel demand forecast modeling and analysis of explanatory variable effects is practical.
基金The Major Project of the National Natural Science Foundation of China(No.50838003)the National Key Technology R&D Program of China during the 11th Five-Year Plan Period(No.2006BAJ02A06)
文摘In order to find out how the climatic characteristics affect people＇s adaptability to thermal environments,experimental studies in a climate chamber are conducted on the effects of transition seasons（from spring to summer）and the occupants＇ native areas on indoor thermal sensations.Results reveal that people＇s tolerances to cool and warm indoor environments are different in the transition season.When the outdoor temperature is higher,the occupants have a weaker tolerance to a cool indoor environment,but a stronger tolerance to a warm indoor environment.Besides,it is found that the occupants＇ thermal sensations depend on both the climatic characteristics of the season and their native areas.The people from southern China present a greater tolerance to both warm and cool indoor environments than those from northern China.The reason can be explained according to the occupants＇ adaptability to the climatic characteristics and the indoor thermal environments of their native areas in different climate zones.
基金Projects(11272267,11102168,10932008)supported by the National Natural Science Foundation of ChinaProject(B07050)supported by Northwestern Polytechnical University
文摘Uniaxial compressive experiments of ultrafine-grained Al fabricated by equal channel angular pressing(ECAP) method were performed at wide temperature and strain rate range. The influence of temperature on flow stress, strain hardening rate and strain rate sensitivity was investigated experimentally. The results show that both the effect of temperature on flow stress and its strain rate sensitivity of ECAPed Al is much larger than those of the coarse-grained Al. The temperature sensitivity of ultrafine-grained Al is comparatively weaker than that of the coarse-grained Al. Based on the experimental results, the apparent activation volume was estimated at different temperatures and strain rates. The forest dislocation interactions is the dominant thermally activated mechanism for ECAPed Al compressed at quasi-static strain rates, while the viscous drag plays an important role at high strain rates.
基金the National Basic Research Development Program of China (No. 2006cb500706)the National Natural Science Foundation of China (No. 30700251)+1 种基金Shanghai Key Project of Basic Science Research (No. 04DZ14005)the Program for Outstanding Medical Academic Leader (No. LJ 06003).
文摘Inflammation has been shown to play an important role in the progression of Alzheimer＇s disease （AD）. Recent epidemical study indicates that the incidence of AD in some populations is substantially influenced by the gene polymorphisms of the inflammation mediators. Meanwhile, an ensured risk factor, the ApoE ε4 allele is also reported to directly promote inflammation. Accordingly, it appears that an individual genetic background has partly determined his predisposition for AD by the extent of the inflammation response to the chronic stimulus by β-amyloid peptide （Aβ） deposits and other antigen stressor in the elderly. Hence we present a hypothesis that the inflammation genotypes may contribute to AD susceptibility. This may provide a new orientation both for future identification of individuals at risk and for personalized medication.
文摘Eight indica ( Oryza sativa L.) environment-sensitive genic male-sterile (EGMS) lines, 2-2S, K1405S, F131S, 2136S, Pei-Ai 64S, 1290S, GD-IS and N17S, were sequentially seeded with 10-15 d interval at three sites, Wuhan in 1997, Guiyang in 1997 and Sanya in 1997 and 1998, China. The results of investigations on self-sterilities showed that all of eight EGMS lines had stable sterile periods of longer than 30 d at Wuhan. They can be used for seed production of two-line hybrid rice, but can not reproduce themselves. Their stable sterile periods were shorter than 30 d at Guiyang, they can reproduce themselves and can not be used for hybrid seed production. In Sanya, their stable sterile periods were longer than 150 d, all of eight lines can be used for seed production in summer and autumn and reproduce themselves in winter. The fertility of all eight lines were sensitive to temperature. The sensitive stages, sensitive duration and critical point of temperatures (CPT) of fertility alteration in various lines were different. The sensitive stages of 2-2S and K1405S were from 18 d to 9 d before heading, the sensitive durations were 7-10 d and the CPTs were 23.7-24.5 degreesC. The sensitive stage, sensitive duration and CPT of F131S were from 17 to 5 d before heading, 13 d and 24.3-24.7 degreesC, respectively. The sensitive stage, sensitive duration and CPT of 2136S were from 18 to 12 d before heading, 7 d and 24.6-25.1 degreesC:, respectively. The sensitive stages, sensitive durations of Pei-Ai 64S, 1290S, N17S and GD-1S were from 24 to 13 d before heading and 10-13 d. And their CPTs were 24.6-25.1 degreesC, 25.5-26.2 degreesC, 25.4-26.1 degreesC,, and 24.1-24.7 degreesC, respectively.
