The effect of gonadal hormones on brain tubulin synthesis has been studied in the castrated middle-aged male mouse and testes intact oldaged male mouse.Estrogen exerts potent influences on stimulating tubulin synthesi...The effect of gonadal hormones on brain tubulin synthesis has been studied in the castrated middle-aged male mouse and testes intact oldaged male mouse.Estrogen exerts potent influences on stimulating tubulin synthesis.Treatment with estradiol benzoate or estradiol benzoate and hydroxyprogesterone caproate increase the amount of tubulin by almost 37%-43%.Administration of testosterone propionate can produce a 13%-23% increase.Both estrogen and androgen can stimulate tubulin synthesis after the critical period of brain development but, estrogen plays a stronger role than androgen in stimulating tubulin synthesis in male mouse.展开更多
AIM: To investigate the effect of glutamine (Gln)-containing parenteral nutrition on phagocytic activity and to elucidate the possible roles of Gln in the secretion of anabolic hormones and nitrogen balance in rats un...AIM: To investigate the effect of glutamine (Gln)-containing parenteral nutrition on phagocytic activity and to elucidate the possible roles of Gln in the secretion of anabolic hormones and nitrogen balance in rats undergoing a gastrectomy. METHODS: Rats with an internal jugular catheter were divided into 2 experimental groups and received total parenteral nutrition (TPN). The TPN solutions were isonitrogenous and identical in nutrient compositions except for differences in amino acid content. One group received conventional TPN (control), and in the other group, 25% of the total amino acid nitrogen was replaced with Gin. After receiving TPN for 3 d, one-third of the rats in each experimental group were sacrificed as the baseline group. The remaining rats underwent a partial gastrectomy and were killed 1 and 3 d, respectively, after surgery. Plasma, peritoneal lavage fluid (PLF), and urine samples were collected for further analysis. RESULTS: The Gln group had fewer nitrogen losses 1 and 2 d after surgery (d1, 16.6±242.5 vs-233.4±205.9 mg/d, d2, 31.8±238.8 vs-253.4±184.6 mg/d, P<0.05). There were no differences in plasma growth hormone (GH) and insulin-like growth factor-1 levels between the 2 groups before or after surgery. The phagocytic activity of peritoneal macrophages was higher in the Gln group than in the control group 1 d after surgery (A 1185±931 vs323±201, P<0.05). There were no differences in the phagocytic activities of blood polymorphonuclear neutrophils between the 2 groups at the baseline or on the postoperative days. No significant differences in interleukin-1β or interleukin-6 concentrations in PLF were observed between the 2 groups. However, tumor necrosis factor-α level in PLF was significantly lower in the Gln group than in the control group on postoperative d 3. CONCLUSION: TPN supplemented with Gln can improve the nitrogen balance, and enhance macrophage phagocytic activity at the site of injury. However, Gin supplementation has no effect on phagocytic cell activity in the systemic circulation, GH and insulin-like growth factor-1 might not be responsible for attenuating nitrogen losses in rats with a partial gastrectomy.展开更多
Objective: To study the effects of quercetin on hepatocyte stimulating factor production from mouse peritoneal macrophages. Methods: Hepatocyte stimulating factor was evaluated by the amount of fibrinogen synthesized ...Objective: To study the effects of quercetin on hepatocyte stimulating factor production from mouse peritoneal macrophages. Methods: Hepatocyte stimulating factor was evaluated by the amount of fibrinogen synthesized in Hep3B cells. Interleukin-6 activity was measured by B9 cell proliferation methyl thiazolyl tetrazolium colorimetric method. Hep3B cell supernatant fibrinogen was quantitated with ELISA. Results: LPS induced the synthesis of hepatocyte stimulating factor in mouse peritoneal macrophages, and hepatocyte stimulating factor promotes the synthesis of fibrinogen from Hep3B cells. Quercetin(5 to 40μmol/ L) inhibited the synthesis of hepatocyte stimulating factor stimulated by LPS. Quercetin(5 to 20μmol/ L) inhibited release of interleukin-6 from mouse peritoneal macrophages induced by 0. 5 g/ L fibrin fibrinogen degradation products. Conclusion: Quercetin inhibits the synthesis of hepatocyte stimulating factor in macrophages.展开更多
The effect of epidermal growth factor(EGF) on follicular development and steroidogenesis was investigated using an in vitro perfused immature rat ovary model. Type 3a and type 3b follicles were counted in the section...The effect of epidermal growth factor(EGF) on follicular development and steroidogenesis was investigated using an in vitro perfused immature rat ovary model. Type 3a and type 3b follicles were counted in the sections of perfused ovaries. To determine action of EGF on amortize activity, effect of testosterone(10-5 M) plus EGF(10 ng/ml) and that of testosterone(10-5 M) alone were compared. EGF(10 ng/ml) significantly increased the number of both types of follicles. At time-course experiment, EGF(10 ng/ml) did not enhance the number of both types of follicles after 10 hr perfusion, but significantly augmented it after perfusion of 20 hrs. EGF(10 ng/m) also stimulated progesterone production. There was no significant difference in estradiol level when ovaries were treated with EGF alone. The addition of testosterone significantly increased estradiol production. EGF inhibited testosterone-derived ovary estradiol production. These results suggest that EGF induces development of the primordial and primary follicles and plays an important role in controlling development by regulating function of granulosa cells.展开更多
The effect of sex hormones on the biogenesis of brain tubulin has been studied in the ovariectomized middle-aged and ovary-intact old-aged female mouse.The results show the much stronger role estrogen plays in stimula...The effect of sex hormones on the biogenesis of brain tubulin has been studied in the ovariectomized middle-aged and ovary-intact old-aged female mouse.The results show the much stronger role estrogen plays in stimulating tubulin synthesis than testosterone. Estradiol increased the contents of brain tubulin by 15%-41%. Estradiol and progesterone together can produce a 23%-49% increase. Maybe this experiment revealed a kind of new action of estrogen, i.e., stimulates brain tubulin syntheses in adult mouse.It is important that the effect of estrogen on stimulating tubulin synthesis takes place after the critical period of brain development. All of these mean that estrogen still plays its role in stimulating tubulin synthesis wile thyroid hormones can't play this kind of role during this time.展开更多
Objective： To investigate male reproductive parameters via changes of potential testicular protein markers in restraint-stress rats. Methods： Male Sprague-Dawley rats were divided into two groups （non-immobilized c...Objective： To investigate male reproductive parameters via changes of potential testicular protein markers in restraint-stress rats. Methods： Male Sprague-Dawley rats were divided into two groups （non-immobilized control and restraint-immobilized/stress groups, n=8 each group）. The stress animals were immobilized （12 h/d） by a restraint cage for 7 consecutive days. All reproductive parameters, morphology and histology were observed and compared between groups. In addition, the expression of steroidogenic acute regulatory （STAR） and phosphotyrosine proteins （previously localized in Sertoli and late spermatid cells） in testicular lysate was assayed by immuno-Western blotting. Results： Testosterone level, sperm concentration and sperm head normality of stress rats were significantly decreased while the corticosterone level was increased as compared with the control （P〈0.05）. Histologically, stress rats showed low sperm mass in epididymal lumen and some atrophy of seminiferous tubules. Although the expression of testicular STAR protein was not significantly different between groups, changed patterns of the 131, 95, and 75 kDa testicular phosphorylated proteins were observed in the stress group compared with the control group. The intensity of a tes- ticular 95-kDa phosphorylated protein was significantly decreased in stress rats. Conclusions： This study has demonstrated the alteration of testicular phosphorylated protein patterns, associated with adverse male reproductive parameters in stress rats. It could be an explanation of some infertility in stress males.展开更多
文摘The effect of gonadal hormones on brain tubulin synthesis has been studied in the castrated middle-aged male mouse and testes intact oldaged male mouse.Estrogen exerts potent influences on stimulating tubulin synthesis.Treatment with estradiol benzoate or estradiol benzoate and hydroxyprogesterone caproate increase the amount of tubulin by almost 37%-43%.Administration of testosterone propionate can produce a 13%-23% increase.Both estrogen and androgen can stimulate tubulin synthesis after the critical period of brain development but, estrogen plays a stronger role than androgen in stimulating tubulin synthesis in male mouse.
基金Supported by Research Grant from National Science Council,Taipei, Taiwan, China No. 91-2815-C-038-007-B
文摘AIM: To investigate the effect of glutamine (Gln)-containing parenteral nutrition on phagocytic activity and to elucidate the possible roles of Gln in the secretion of anabolic hormones and nitrogen balance in rats undergoing a gastrectomy. METHODS: Rats with an internal jugular catheter were divided into 2 experimental groups and received total parenteral nutrition (TPN). The TPN solutions were isonitrogenous and identical in nutrient compositions except for differences in amino acid content. One group received conventional TPN (control), and in the other group, 25% of the total amino acid nitrogen was replaced with Gin. After receiving TPN for 3 d, one-third of the rats in each experimental group were sacrificed as the baseline group. The remaining rats underwent a partial gastrectomy and were killed 1 and 3 d, respectively, after surgery. Plasma, peritoneal lavage fluid (PLF), and urine samples were collected for further analysis. RESULTS: The Gln group had fewer nitrogen losses 1 and 2 d after surgery (d1, 16.6±242.5 vs-233.4±205.9 mg/d, d2, 31.8±238.8 vs-253.4±184.6 mg/d, P<0.05). There were no differences in plasma growth hormone (GH) and insulin-like growth factor-1 levels between the 2 groups before or after surgery. The phagocytic activity of peritoneal macrophages was higher in the Gln group than in the control group 1 d after surgery (A 1185±931 vs323±201, P<0.05). There were no differences in the phagocytic activities of blood polymorphonuclear neutrophils between the 2 groups at the baseline or on the postoperative days. No significant differences in interleukin-1β or interleukin-6 concentrations in PLF were observed between the 2 groups. However, tumor necrosis factor-α level in PLF was significantly lower in the Gln group than in the control group on postoperative d 3. CONCLUSION: TPN supplemented with Gln can improve the nitrogen balance, and enhance macrophage phagocytic activity at the site of injury. However, Gin supplementation has no effect on phagocytic cell activity in the systemic circulation, GH and insulin-like growth factor-1 might not be responsible for attenuating nitrogen losses in rats with a partial gastrectomy.
基金Supported by the National Natural Science Foundation of China(No.39370798,30200344)
文摘Objective: To study the effects of quercetin on hepatocyte stimulating factor production from mouse peritoneal macrophages. Methods: Hepatocyte stimulating factor was evaluated by the amount of fibrinogen synthesized in Hep3B cells. Interleukin-6 activity was measured by B9 cell proliferation methyl thiazolyl tetrazolium colorimetric method. Hep3B cell supernatant fibrinogen was quantitated with ELISA. Results: LPS induced the synthesis of hepatocyte stimulating factor in mouse peritoneal macrophages, and hepatocyte stimulating factor promotes the synthesis of fibrinogen from Hep3B cells. Quercetin(5 to 40μmol/ L) inhibited the synthesis of hepatocyte stimulating factor stimulated by LPS. Quercetin(5 to 20μmol/ L) inhibited release of interleukin-6 from mouse peritoneal macrophages induced by 0. 5 g/ L fibrin fibrinogen degradation products. Conclusion: Quercetin inhibits the synthesis of hepatocyte stimulating factor in macrophages.
文摘The effect of epidermal growth factor(EGF) on follicular development and steroidogenesis was investigated using an in vitro perfused immature rat ovary model. Type 3a and type 3b follicles were counted in the sections of perfused ovaries. To determine action of EGF on amortize activity, effect of testosterone(10-5 M) plus EGF(10 ng/ml) and that of testosterone(10-5 M) alone were compared. EGF(10 ng/ml) significantly increased the number of both types of follicles. At time-course experiment, EGF(10 ng/ml) did not enhance the number of both types of follicles after 10 hr perfusion, but significantly augmented it after perfusion of 20 hrs. EGF(10 ng/m) also stimulated progesterone production. There was no significant difference in estradiol level when ovaries were treated with EGF alone. The addition of testosterone significantly increased estradiol production. EGF inhibited testosterone-derived ovary estradiol production. These results suggest that EGF induces development of the primordial and primary follicles and plays an important role in controlling development by regulating function of granulosa cells.
文摘The effect of sex hormones on the biogenesis of brain tubulin has been studied in the ovariectomized middle-aged and ovary-intact old-aged female mouse.The results show the much stronger role estrogen plays in stimulating tubulin synthesis than testosterone. Estradiol increased the contents of brain tubulin by 15%-41%. Estradiol and progesterone together can produce a 23%-49% increase. Maybe this experiment revealed a kind of new action of estrogen, i.e., stimulates brain tubulin syntheses in adult mouse.It is important that the effect of estrogen on stimulating tubulin synthesis takes place after the critical period of brain development. All of these mean that estrogen still plays its role in stimulating tubulin synthesis wile thyroid hormones can't play this kind of role during this time.
基金Project supported by the Postgraduate Study Support Grant and Invitation Research Grant(IN59134),Faculty of MedicineKhon Kaen University,Khon Kaen,Thailand
文摘Objective： To investigate male reproductive parameters via changes of potential testicular protein markers in restraint-stress rats. Methods： Male Sprague-Dawley rats were divided into two groups （non-immobilized control and restraint-immobilized/stress groups, n=8 each group）. The stress animals were immobilized （12 h/d） by a restraint cage for 7 consecutive days. All reproductive parameters, morphology and histology were observed and compared between groups. In addition, the expression of steroidogenic acute regulatory （STAR） and phosphotyrosine proteins （previously localized in Sertoli and late spermatid cells） in testicular lysate was assayed by immuno-Western blotting. Results： Testosterone level, sperm concentration and sperm head normality of stress rats were significantly decreased while the corticosterone level was increased as compared with the control （P〈0.05）. Histologically, stress rats showed low sperm mass in epididymal lumen and some atrophy of seminiferous tubules. Although the expression of testicular STAR protein was not significantly different between groups, changed patterns of the 131, 95, and 75 kDa testicular phosphorylated proteins were observed in the stress group compared with the control group. The intensity of a tes- ticular 95-kDa phosphorylated protein was significantly decreased in stress rats. Conclusions： This study has demonstrated the alteration of testicular phosphorylated protein patterns, associated with adverse male reproductive parameters in stress rats. It could be an explanation of some infertility in stress males.
