林木重要性状形成的分子基础研究进展

林业是国民经济的重要产业之一,深入研究以人类需求为目的的林木重要性状,解析其形成的分子基础,是建立高效育种技术体系的基本保障,对培育满足国家重大需求的突破性良种,保障我国林业可持续发展,服务国家木材安全、粮食安全、生态安全和乡村振兴等重大战略具有重要意义。随着生物技术的快速发展,针对林木性状形成的分子基础研究也更加深入。综述了国内外林木性状形成的分子基础,聚焦木材形成、林木经济性状和林木抗逆性的形成与调控机制,对当前的研究进展进行了梳理,并对未来林木性状研究趋势与技术发展进行了展望。

转基因与进化生态学研究

采用转基因步骤 ,从遗传上改变个体的某一性状并观察它在存活、生长、生殖、防御或竞争能力上的相应变化 ,是突破常规方法的局限及揭示生物在进化历程中表型性状的形成与自身存活。

Statistic Morphology Difference between Male and Female Individuals of Paramisgurnus dabryanus Sauvage

[Objective] The aim was to compare the morphology difference between male and female individuals of Paramisgurnus dabryanus Sauvage. [Method] The comparison of 10 morphological characteristics between male and female individuals of P. dabryanus was conducted with principal component analysis and t-test methods. [Result] The results of t-test indicated that three of 10 morphological characteristics showed extremely significant difference（P0.01）and one of 10 morphological characteristics was significantly different（P0.05）. Principal component analysis revealed four principal components,the contribution rate of which was 31.151%,18.844%,12.324% and 10.169%,respectively. The cumulative contribution rate had reached 72.487%. The morphological variations between male and female individuals were not significant,which was consistent with that of t-test. [Conclusion] The result in this study had provided theoretical basis for the breeding of P. dabryanus.

Finite element analysis and simulation for cold precision forging of a helical gear

To investigate the effects of billet geometry on the cold precision forging process of a helical gear, six different billet geometries were designed utilizing the relief-hole principle. And the influences of the billet geometry on the forming load and the deformation uniformity were analyzed by three-dimensional （3D） finite element method （FEM） under the commercial software DEFORM 3D. The billet geometry was optimized to meet lower forming load and better deformation uniformity requirement. Deformation mechanism was studied through the distribution of flow velocity field and effective strain field. The forging experiments of the helical gear were successfully performed using lead material as a model material under the same process conditions used in the FE simulations. The results show that the forming load decreases as the diameter of relief-hole do increases, but the effect of do on the deformation uniformity is very complicated. The forming load is lower and the deformation is more uniform when do is 10 mm.

Formability analysis of dissimilar tailor welded blanks welded with different tool pin profiles

An attempt has been made to study the tailor welded blanks （TWBs） made by friction stir welding （FSW） with five different tool pin profiles. The formability of welded blanks was evaluated at constant FSW process parameters. It has been found that the welded blanks with square pin profile tool produced better formability properties as compared to other tool pin profiles. It was found that the pulsating action of the square pin profile is said to be the reason for this improvement. A microstructural evaluation performed on the blanks revealed that the stir zone created by taper cylindrical and stepped cylindrical tools was less homogeneous than that of the square tool, resulting in less formability. The limiting dome height （LDH） of square pin profile tool was found to be 14 mm while those of taper cylindrical and stepped cylindrical tools are 12.5 mm and 12.4 mm, respectively.

Morphological comparison between a selected fast-growing strain and the common cultured strain of turbot Scophthalmus maximus

To evaluate the effect of genetic improvement of the turbot Scophthalmus maximus, we analyzed morphological differences between a fast-growing strain obtained by family selection and the common cultured strain, by principal component analysis, stepwise discriminant analysis, and t-tests. Although they clearly differed morphologically, plots of the principal components of the two strains partially overlapped. However, the difference between the strains was supported with very high precision by discriminant analysis. The t-tests revealed that 4 of the 13 morphological traits analyzed were highly significantly different (P<0.01), 4 traits also differed significantly (P<0.05), and the remainder did not differ significantly. The coefficients of difference of the 13 traits were all lower than the threshold value between subspecies (1.28). Together, the results indicate that a trend for segregation of characters from the common cultured strain have already appeared in the selected fast-growing strain but the degree of segregation have not risen to subspecies level.

Comparative Analysis of Morphologic Traits of 50 Large-flowered Chrysanthemum Varieties

With 50 large-flowered Chrysanthemum varieties from germplasm nursery of Wunaoshan Forest Farm in Macheng City as research objects, 64 morphological traits were investigated by field experiments adopting randomized block design. The morphological differences were observed by uniformity analysis, variability analysis, principal component analysis and cluster analysis. The result showed that the vari- able coefficients of 59 traits were greater than 15%; the contribution rate of first seven principal components reached 81.45%; and it was found by clustering analy- sis that the 50 germplasm resources could be divided into four clusters with obvious morphological differences, and plant type could be used as an index for classifica- tion.

Morphological Study of Ficus deltoidea Jack in Malaysia

Ficus deltoidea Jack （Moraceae） or Mas Cotek is a small shrub with a great morphological variation. Measurement of 40 morphological traits had been done on 50 accessions to find the most significant characters that enable differentiation being done according to its variety groups. The data were analyzed with principal component analysis （PCA） and cluster analysis （CA） using cluster software package programme to produce the scatter diagram and dendrogram relationship of the taxa. The results showed that there were 25 morphological characters having the value of factor analysis greater than 0.60 from its principal component （PC） with the Eigen value greater than 1.0. 16 out of 40 morphological characters had been identified as having high values of correlation coefficient ranging from -0.783 to 0.890. The analysis showed that the most responsible characters in grouping the samples into different groups are the shape and size of leaf, number and color of dots on the leaf surface and characters of syconium. The scatter diagram of the accessions on the PC1 against PC2 showed six major groups. The dendrogram displayed the relationship among the accessions and within the dissimilarity distance = 19, it classified the samples into five major groups. Observation on F. deltoidea resulted in the findings of high variability among the accessions. The most significant characters in grouping accessions are the shapes of leaf base （BL）, shape of leaf apex （SA）, ratio of lamina width to lamina length （R）, dots color at the lower midrib （DLM）, color of young syconium （CYS）, color of mature syconium （CMS） and the number of syconium on trees （DST）. This study provides basic information for introduction of some particular traits and effective conservation of the species breeding programme. The morphological traits were found to be useful for the diversity studies and in identifying the variation. The actual figures of F. deltoidea obtained through this study enable comparison being done to the previous and in future study. Hence, the varieties that are extinct could be recognised.

Acute myocardial infarction in patients of nephrotic syndrome： a case series

Thromboembolic complications have been frequently re- ported in patients with long-lasting nephrotic syndrome （NS）. Although thrombotic complications in the venous system are common in patients with NS, arterial thromboses associated with NS are much less common. However, coronary thromboses are extremely rarely observed. So, NS is a rare cause of acute coronary syndrome （ACS）. As such, the incidence, pathogenesis, and treatment of these patients have yet to be clearly defined. In the current litera- ture, publications contain less than 15 patients, most of whom are young children.

Leaf stable carbon isotope composition in Picea schrenkiana var. tianschanica in relation to leaf physiological and morphological characteristics along an altitudinal gradient

To understand the effects of leaf physiological and morphological characteristics on δ13C of alpine trees, we examined leaf δ13C value, LA, SD, LNC, LPC, LKC, Chla+b, LDMC, LMA and Narea in one-year-old needles of Picea schrenkiana var. tianschanica at ten points along an altitudinal gradient from 1420 m to 2300 m a.s.l. on the northern slopes of the Tianshan Mountains in northwest China. Our results indicated that all the leaf traits differed significantly among sampling sites along the altitudinal gradient(P<0.001). LA, SD, LPC, LKC increased linearly with increasing elevation, whereas leaf δ13C, LNC, Chla+b, LDMC, LMA and Narea varied non-linearly with changes in altitude. Stepwise multiple regression analyses showed that four controlled physiological and morphological characteristics influenced the variation of δ13C. Among these four controlled factors, LKC was the most profound physiological factor that affected δ13C values, LA was the secondary morphological factor, SD was the third morphological factor, LNC was the last physiological factor. This suggested that leaf δ13C was directly controlled by physiological and morphological adjustments with changing environmental conditions due to the elevation.

Gene editing: an instrument for practical application of gene biology to plant breeding

Plant breeding is well recognized as one of the most important means to meet food security challenges caused by the ever-increasing world population. During the past three decades, plant breeding has been empowered by both new knowledge on trait development and regulation(e.g., functional genomics) and new technologies(e.g., biotechnologies and phenomics). Gene editing, particularly by clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein(Cas) and its variants, has become a powerful technology in plant research and may become a game-changer in plant breeding. Traits are conferred by coding and non-coding genes. From this perspective, we propose different editing strategies for these two types of genes. The activity of an encoded enzyme and its quantity are regulated at transcriptional and post-transcriptional, as well as translational and post-translational, levels. Different strategies are proposed to intervene to generate gene functional variations and consequently phenotype changes. For non-coding genes, trait modification could be achieved by regulating transcription of their own or target genes via gene editing. Also included is a scheme of protoplast editing to make gene editing more applicable in plant breeding. In summary, this review provides breeders with a host of options to translate gene biology into practical breeding strategies, i.e., to use gene editing as a mechanism to commercialize gene biology in plant breeding.

Reinforcement as an initiator of population Jivergence and speciation

When hybridization results in reduced fitness, natural selection is expected to favor the evolution of traits that minimize the likelihood of hybridizing in the first place. This process, termed reinforcement （or, more generally, reproductive character displacement）, thereby contributes to the evolution of enhanced reproductive isolation between hybridizing groups. By enhancing reproductive isolation in this way, reinforcement plays an important role in the final stages of speciation. However, reinforcement can also contribute to the early stages of speciation. Specifically, because selection to avoid hybridization occurs only in sympatric populations, the unfolding of reinforcement can lead to the evolution of traits in sympatric populations that reduce reproduction between conspecifics in sympatry versus those in allopatry. Thus, reinforcement between species can lead to reproductive isolation--and possibly speciation-between populations in sympatry versus those in allopatry or among different sympatric populations. Here, I describe how this process can occur, the conditions under which it is most likely to occur, and the empirical data needed to evaluate the hypothesis that reinforcement can initiate speciation.

A New Dynamic Model for a Flexible Hub-Beam System

In this paper,a new dynamic model for the flexible hub-beam system is proposed by using the principle of continuum medium mechanics and the finite element discretization method.In the proposed model,the coupling deformation of any element of the beam is only related with the nodal coordinates of this element.So this model is suitable to the rotating beam in an arbitrary shape.Numerical examples of slender beams in straight and irregular shapes are carried out to demonstrate the validation of the proposed model.Simulation results indicate that the proposed model can be used valid for dynamic description of flexible rotating beam in irregular shape, and for both low and high rotation speeds.