以12 000 k N船用构件力学性能检测平台油缸座为研究对象,根据测试平台的尺寸和实现的功能设计了油缸座结构,对相关参数进行计算,给出其三维模型;利用有限元方法对油缸座进行了静力学分析与冲击动力学分析。分析结果表明,油缸座在极限...以12 000 k N船用构件力学性能检测平台油缸座为研究对象,根据测试平台的尺寸和实现的功能设计了油缸座结构,对相关参数进行计算,给出其三维模型;利用有限元方法对油缸座进行了静力学分析与冲击动力学分析。分析结果表明,油缸座在极限静载荷及冲击载荷作用下的最大应力均未超过材料屈服极限,验证了油缸座结构的合理性。当试样断裂时,油缸座的应力峰值比静力学分析结果提高了54%左右,因此对于大型结构试验机而言,有必要对关键受力零部件进行冲击动力学分析。展开更多
To develop the high-performance fluorescent bio-sensors, the metal nanoparticles were employed as nanoquenchers and at- tracted reasonable attention in the design of fluorescent biosensors. In this work, silver nanopa...To develop the high-performance fluorescent bio-sensors, the metal nanoparticles were employed as nanoquenchers and at- tracted reasonable attention in the design of fluorescent biosensors. In this work, silver nanoparticles (AgNPs) were obtained via reduction of Ag+ on FAM-labeled DNA template. For the tight binding between AgNPs and DNA, the tem- plate-synthesized AgNPs turned out high quenching efficiency and could be applied as super nanoquenchers to establish the biosensing platform for fluorescent detection. As an example, the template-synthesized DNA-AgNPs conjugates were em- ployed in sensing thiols. By forming S-Ag bonds, thiols interact intensely with AgNPs and replace the FAM-labeled DNA off from the surface of AgNPs, resulting in a fluorescence enhancement. Besides the advantages of lower background and higher signal-to-background ratio (S/B), the conjugates present better stability, making them applicable in complicated biological fluids. To further evidence the feasibility of sensing thiols in real samples, the thiols in human urine were detected. The total amount of free thiols found in human urine was ranging from 229 μM to 302μM with the proposed sensor. To conclude the reliability, low content of Cys was added and the recovery was 98%-103%.展开更多
文摘以12 000 k N船用构件力学性能检测平台油缸座为研究对象,根据测试平台的尺寸和实现的功能设计了油缸座结构,对相关参数进行计算,给出其三维模型;利用有限元方法对油缸座进行了静力学分析与冲击动力学分析。分析结果表明,油缸座在极限静载荷及冲击载荷作用下的最大应力均未超过材料屈服极限,验证了油缸座结构的合理性。当试样断裂时,油缸座的应力峰值比静力学分析结果提高了54%左右,因此对于大型结构试验机而言,有必要对关键受力零部件进行冲击动力学分析。
基金supported by the National Natural Foundation of China (21075032 & 21005026)National Key Basic Research Program (2011CB911000)Hunan Province Key Project of Scientific & Tech-nical Programs (2010TP4013-1)
文摘To develop the high-performance fluorescent bio-sensors, the metal nanoparticles were employed as nanoquenchers and at- tracted reasonable attention in the design of fluorescent biosensors. In this work, silver nanoparticles (AgNPs) were obtained via reduction of Ag+ on FAM-labeled DNA template. For the tight binding between AgNPs and DNA, the tem- plate-synthesized AgNPs turned out high quenching efficiency and could be applied as super nanoquenchers to establish the biosensing platform for fluorescent detection. As an example, the template-synthesized DNA-AgNPs conjugates were em- ployed in sensing thiols. By forming S-Ag bonds, thiols interact intensely with AgNPs and replace the FAM-labeled DNA off from the surface of AgNPs, resulting in a fluorescence enhancement. Besides the advantages of lower background and higher signal-to-background ratio (S/B), the conjugates present better stability, making them applicable in complicated biological fluids. To further evidence the feasibility of sensing thiols in real samples, the thiols in human urine were detected. The total amount of free thiols found in human urine was ranging from 229 μM to 302μM with the proposed sensor. To conclude the reliability, low content of Cys was added and the recovery was 98%-103%.