AIM: To compare the results for endoscopic ultrasound (EUS)-guided drainage of clear fluid pancreatic pseudocysts with the results for abscess drainage. METHODS: All patients referred for endoscopic drainage of a flui...AIM: To compare the results for endoscopic ultrasound (EUS)-guided drainage of clear fluid pancreatic pseudocysts with the results for abscess drainage. METHODS: All patients referred for endoscopic drainage of a fluid collection were prospectively included. The outcome was recorded. RESULTS: Altogether 26 pseudocysts or abscesses were treated in 25 (6 female) patients. One endoscopist performed the procedures. Non-infected pseudocysts were present in 15 patients and 10 patients had infected fluid collections. The cyst size ranged between 28 cm × 13 cm and 5 cm × 5 cm. The EUS drainage was successful in 94% of the pseudocysts and in 80% of the abscesses (P = 0.04). The complication rate in pseudocysts was 6% and in abscesses was 30% (P = 0.02). Recurrence of a pseudocyst occurred in one patient (4%) after 6 mo; the patient was successfully retreated. CONCLUSION: EUS-guided drainage of pseudocysts is associated with a higher success rate and a lower complication rate compared with abscess drainage.展开更多
The present trial was conducted with starry flounder Platichthys stellatus broodstock to evaluate the effects of dietary vitamin A, ascorbic acid, and a-tocopherol on the gonadal development and reproductive performan...The present trial was conducted with starry flounder Platichthys stellatus broodstock to evaluate the effects of dietary vitamin A, ascorbic acid, and a-tocopherol on the gonadal development and reproductive performance. 8 000 IU/kg diet vitaminA （VA group）, 500 mg/kg diet ascorbic acid （Vcpp group）, or 250 mg/kg diet a-tocopherol （a-TA group） was added into basal diet to create 3 vitamin experimental diets, respectively. Each diet was fed to 450 starry flounder broodstock for 104 days. Samples were collected weekly. The gonadosomatic index （GSI） of 3 vitamin experimental groups first decreased and then increased. Maximum GSI of Vcpp group was higher than that of ct-TA group but lower than that of VA group. The spawning periods of 3 vitamin experimental groups lasted 49, 56, and 45 days, respectively. No mature eggs were observed in the control group during the trial. The absolute fecundity （AF） and relative fecundity （RF） of a-TA group was higher than that of Vcpp group but lower than that of VA group. The results suggest that different vitamins play different roles in the fish reproductive process. Vitamin A stimulated the maturation of the ovary, ascorbic acid prolonged the spawning period, and a-tocopherol affected the development of the eggs.展开更多
The limited knowledge about the relationships between environmental factors and the maturation and reproductive seasonality of nemerteans is mostly obtained from field studies, and in many cases is conjectural. Few sp...The limited knowledge about the relationships between environmental factors and the maturation and reproductive seasonality of nemerteans is mostly obtained from field studies, and in many cases is conjectural. Few species have been studied under well-controlled laboratory conditions. In this work, the effects of temperature and photoperiod on gonad development and spawning of Cephalothrix hongkongiensis were observed by rearing worms under different temperatures and photoperiods. Worms reared at 4~C and 6~C showed no growth or gonadal development, and all died within 109 days. At 8~C and 12~C, gonads could be seen from 90 days to the termination of the 360-day experiment, but no spawning was observed. Worms maintained at 16, 24, and 28~C showed both gonadal development and spawning, and matured faster at higher temperatures. Under a fixed temperature （16℃）, photoperiod did not have any significant impact on maturation and spawning. It is likely that temperature plays a major role in regulating the maturation and reproductive cycle of C. hongkongiensis.展开更多
A twelve week experiment was conducted to compare the effects of diets with plant-based and animal-based ingredients on growth and gonad development of Clarias gariepinus. One hundred and 12 sub-adult C. gariepinus wi...A twelve week experiment was conducted to compare the effects of diets with plant-based and animal-based ingredients on growth and gonad development of Clarias gariepinus. One hundred and 12 sub-adult C. gariepinus with an average weight of 205 ×5.09 g, were stocked in six concrete tanks （9 × 4 × 2 m3） containing 20 fish each. Experiment had 3 replicates and animals fed daily at 5% body weights. There was no significant difference （P 〈 0.05） in proximate compositions of the two experimental feeds and controls. Fecundity, gonad weight and gonasomatic index were higher in fish fed diet 13 than A and C. Similarly, growth indices were higher in fish fed diet B than in A and C. Histology of gonads showed a faster development of oocytes of eggs in fish fed animal-based ingredients than plant-based and combined plant and animal diets. Although there were slight differences in growth parameters and gonad development in favor of feed with animal-based ingredients, plant-based feed compared favorably in the growth and gonad development of C. gariepinus. Plant-based ingredients are recommended on the basis of affordability and availability as substitute for animal-based ingredients in C. gariepinus feed.展开更多
IntroductionBreast cancer is one of the most commonly seen malignant tumors in the female population, and it ranks the first in the incidence of malignancies among women. The skeleton is a common metastatic site for a...IntroductionBreast cancer is one of the most commonly seen malignant tumors in the female population, and it ranks the first in the incidence of malignancies among women. The skeleton is a common metastatic site for advanced breast cancer and is where spinal metastasis is most frequently seen. The incidence rate of spinal metastasis in descending order is as follows： thoracic vertebrae, lumbar vertebrae, sacrococcygeal vertebrae and cervical vertebrae.展开更多
Objective To clarify the role of vitamin D receptor (VDR) expression in parathyroid proliferation and resistance of parathyroid glands to 1,25(OH) 2D 3 with secondary hyperparathyroidism (SHPT) Methods This study...Objective To clarify the role of vitamin D receptor (VDR) expression in parathyroid proliferation and resistance of parathyroid glands to 1,25(OH) 2D 3 with secondary hyperparathyroidism (SHPT) Methods This study used archive parathyroid with 7 uremic patients The expression of proliferation cell nuclear antigen (PCNA) and VDR was evaluated in nineteen surgically excised parathyroid tissues, including 11 diffuse hyperplasia (DH type) and 8 nodular hyperplasia (NH type) of parathyroid glands, by immunohistochemistry (avidin biotin complex method) Results The weight of parathyroid in SHPT was remarkably increased by 16 1 times The numbers of parathyroid cells were increased by 1 86 times The rate of PCNA was remarkably increased in parathyroid hyperplasia with SHPT compared with that in control group [(6 35±3 36)‰ vs (1 73±1 31)‰, P <0 001] The number of PCNA in DH type was lower than that in NH type ( P <0 001) The density of VDR in the parathyroid with SHPT was significantly decreased [(40 28±13 13)% vs (83 79±3 77)%, P <0 001], VDR immunoreactivity expression in NH type was lower than that in DH type [(27 14±4 12)% vs (49 84±7 33)%, P <0 001] A significantly negative correlation was found between VDR density and the weight of the parathyroid ( r =-0 46, P <0 05), the same as VDR and PCNA ( r = -0 75, P <0 001) Conclusion VDR density was significantly decreased in parathyroid tissue of uremic patients showing nodular hyperplasia compared with that in diffuse hyperplasia and there was significantly negative correlation between VDR density and the weight of the parathyroid, and this may contribute to the progression of SHPT Furthermore, VDR deficiency may cause the resistance of parathyroid cells to 1, 25(OH) 2D 3, in part展开更多
基金Supported by The Health and Medical Care Executive Board of the Vstra Gtaland Region, Sweden
文摘AIM: To compare the results for endoscopic ultrasound (EUS)-guided drainage of clear fluid pancreatic pseudocysts with the results for abscess drainage. METHODS: All patients referred for endoscopic drainage of a fluid collection were prospectively included. The outcome was recorded. RESULTS: Altogether 26 pseudocysts or abscesses were treated in 25 (6 female) patients. One endoscopist performed the procedures. Non-infected pseudocysts were present in 15 patients and 10 patients had infected fluid collections. The cyst size ranged between 28 cm × 13 cm and 5 cm × 5 cm. The EUS drainage was successful in 94% of the pseudocysts and in 80% of the abscesses (P = 0.04). The complication rate in pseudocysts was 6% and in abscesses was 30% (P = 0.02). Recurrence of a pseudocyst occurred in one patient (4%) after 6 mo; the patient was successfully retreated. CONCLUSION: EUS-guided drainage of pseudocysts is associated with a higher success rate and a lower complication rate compared with abscess drainage.
基金Supported by the Public Science and Technology Research Funds Projects of Ocean(No.201205025)the Platform of Aquatic Nutrition and Feed(No.201304001)the Taishan Scholar Fellowship of Shandong Province
文摘The present trial was conducted with starry flounder Platichthys stellatus broodstock to evaluate the effects of dietary vitamin A, ascorbic acid, and a-tocopherol on the gonadal development and reproductive performance. 8 000 IU/kg diet vitaminA （VA group）, 500 mg/kg diet ascorbic acid （Vcpp group）, or 250 mg/kg diet a-tocopherol （a-TA group） was added into basal diet to create 3 vitamin experimental diets, respectively. Each diet was fed to 450 starry flounder broodstock for 104 days. Samples were collected weekly. The gonadosomatic index （GSI） of 3 vitamin experimental groups first decreased and then increased. Maximum GSI of Vcpp group was higher than that of ct-TA group but lower than that of VA group. The spawning periods of 3 vitamin experimental groups lasted 49, 56, and 45 days, respectively. No mature eggs were observed in the control group during the trial. The absolute fecundity （AF） and relative fecundity （RF） of a-TA group was higher than that of Vcpp group but lower than that of VA group. The results suggest that different vitamins play different roles in the fish reproductive process. Vitamin A stimulated the maturation of the ovary, ascorbic acid prolonged the spawning period, and a-tocopherol affected the development of the eggs.
基金Supported by the National Natural Science Foundation of China(Nos.30970333,31172046)
文摘The limited knowledge about the relationships between environmental factors and the maturation and reproductive seasonality of nemerteans is mostly obtained from field studies, and in many cases is conjectural. Few species have been studied under well-controlled laboratory conditions. In this work, the effects of temperature and photoperiod on gonad development and spawning of Cephalothrix hongkongiensis were observed by rearing worms under different temperatures and photoperiods. Worms reared at 4~C and 6~C showed no growth or gonadal development, and all died within 109 days. At 8~C and 12~C, gonads could be seen from 90 days to the termination of the 360-day experiment, but no spawning was observed. Worms maintained at 16, 24, and 28~C showed both gonadal development and spawning, and matured faster at higher temperatures. Under a fixed temperature （16℃）, photoperiod did not have any significant impact on maturation and spawning. It is likely that temperature plays a major role in regulating the maturation and reproductive cycle of C. hongkongiensis.
文摘A twelve week experiment was conducted to compare the effects of diets with plant-based and animal-based ingredients on growth and gonad development of Clarias gariepinus. One hundred and 12 sub-adult C. gariepinus with an average weight of 205 ×5.09 g, were stocked in six concrete tanks （9 × 4 × 2 m3） containing 20 fish each. Experiment had 3 replicates and animals fed daily at 5% body weights. There was no significant difference （P 〈 0.05） in proximate compositions of the two experimental feeds and controls. Fecundity, gonad weight and gonasomatic index were higher in fish fed diet 13 than A and C. Similarly, growth indices were higher in fish fed diet B than in A and C. Histology of gonads showed a faster development of oocytes of eggs in fish fed animal-based ingredients than plant-based and combined plant and animal diets. Although there were slight differences in growth parameters and gonad development in favor of feed with animal-based ingredients, plant-based feed compared favorably in the growth and gonad development of C. gariepinus. Plant-based ingredients are recommended on the basis of affordability and availability as substitute for animal-based ingredients in C. gariepinus feed.
文摘IntroductionBreast cancer is one of the most commonly seen malignant tumors in the female population, and it ranks the first in the incidence of malignancies among women. The skeleton is a common metastatic site for advanced breast cancer and is where spinal metastasis is most frequently seen. The incidence rate of spinal metastasis in descending order is as follows： thoracic vertebrae, lumbar vertebrae, sacrococcygeal vertebrae and cervical vertebrae.
文摘Objective To clarify the role of vitamin D receptor (VDR) expression in parathyroid proliferation and resistance of parathyroid glands to 1,25(OH) 2D 3 with secondary hyperparathyroidism (SHPT) Methods This study used archive parathyroid with 7 uremic patients The expression of proliferation cell nuclear antigen (PCNA) and VDR was evaluated in nineteen surgically excised parathyroid tissues, including 11 diffuse hyperplasia (DH type) and 8 nodular hyperplasia (NH type) of parathyroid glands, by immunohistochemistry (avidin biotin complex method) Results The weight of parathyroid in SHPT was remarkably increased by 16 1 times The numbers of parathyroid cells were increased by 1 86 times The rate of PCNA was remarkably increased in parathyroid hyperplasia with SHPT compared with that in control group [(6 35±3 36)‰ vs (1 73±1 31)‰, P <0 001] The number of PCNA in DH type was lower than that in NH type ( P <0 001) The density of VDR in the parathyroid with SHPT was significantly decreased [(40 28±13 13)% vs (83 79±3 77)%, P <0 001], VDR immunoreactivity expression in NH type was lower than that in DH type [(27 14±4 12)% vs (49 84±7 33)%, P <0 001] A significantly negative correlation was found between VDR density and the weight of the parathyroid ( r =-0 46, P <0 05), the same as VDR and PCNA ( r = -0 75, P <0 001) Conclusion VDR density was significantly decreased in parathyroid tissue of uremic patients showing nodular hyperplasia compared with that in diffuse hyperplasia and there was significantly negative correlation between VDR density and the weight of the parathyroid, and this may contribute to the progression of SHPT Furthermore, VDR deficiency may cause the resistance of parathyroid cells to 1, 25(OH) 2D 3, in part
