[Objective] The aim of this study was to investigate the content changes and their correlations of the photosynthetic pigment,phenols,including total phenols,total flavonoids and anthocyanins,and total saponins of the...[Objective] The aim of this study was to investigate the content changes and their correlations of the photosynthetic pigment,phenols,including total phenols,total flavonoids and anthocyanins,and total saponins of the one-year-old P.notoginseng plants under supplemental UV-B stress in fields.[Method] The one-year-old plants were irradiated by UV-B in field for 1 min per day,and the plants under the UV-B lamp were regarded as a circle center,achieving an annular leaf-sampling.The photosynthetic pigment,phenols and total saponins of the leaves were determined spectrophotometrically.[Result] With the increase of sampling radius,the supplemental UV-B intensity decreased significantly,the contents of chlorophyll (Chl) a,Chl b,Chl (a+b),carotenoid (Car) and total photosynthetic pigment (Chl+Car) of the leaves increased extremely significantly,the Chl a/b and total phenol content (TPC) decreased extremely significantly,but the Chl (a+b)/Car changes were not significant.The contents of total flavonoids,anthocyanins and saponins all increased due to the increasing of UV-B,displaying dose effects.The UV-B intensity was positively correlated with the Chl a/b,and negatively with the Chl a,Chl b,Chl (a+ b),Car and (Chl+Car) contents; and the two of TPC,total flavonoid content (TFC),total anthocyanin content (TAC) and total saponin content (TSC) were positively correlated,all reaching extremely significant level.The UV-B intensity was positively and significantly correlated with the total flavonoid content (TFC),negatively and significantly with the Chl (a+b)/Car,and positively and insignificantly with the TPC,TAC and TSC.[Conclusion] For one-year-old plants of P.notoginseng,UV-B can decrease the contents of the Chl a,Chl b,Chl (a+b),Car and (Chl+Car) and increase the Chl a/b and TPC,and,furthermore,induce the increases of the TFC,TAC and TSC in a dose-dependent manner.However,UV-B can hardly change the Chl (a+b)/Car.The supplemental UV-B of well-suited dose might be one of the effective measures to improve the TSC of P.notoginseng.展开更多
Phaeodactylum tricornutum is a potential livestock for the combined production of eicosapentaenoic acid(EPA) and fucoxanthin. In this study, six marine diatom strains identified as P. tricornutum were cultured and the...Phaeodactylum tricornutum is a potential livestock for the combined production of eicosapentaenoic acid(EPA) and fucoxanthin. In this study, six marine diatom strains identified as P. tricornutum were cultured and their total lipid, fatty acid composition and major photosynthetic pigments determined. It was found that the cell dry weight concentration and mean growth rate ranged between 0.24–0.36 g/L and 0.31–0.33/d, respectively. Among the strains, SCSIO771 presented the highest total lipid content, followed by SCSIO828, and the prominent fatty acids in all strains were C16:0, C16:1, C18:1, and C20:5(EPA). Polyunsaturated fatty acids, including C16:2, C18:2, and EPA, comprised a significant proportion of the total fatty acids. EPA was markedly high in all strains, with the highest in SCSIO828 at 25.65% of total fatty acids. Fucoxanthin was the most abundant pigment in all strains, with the highest in SCSIO828 as well, at 5.50 mg/g. The collective results suggested that strain SCSIO828 could be considered a good candidate for the concurrent production of EPA and fucoxanthin.展开更多
氮沉降增加将影响草原生态系统固碳,但如何影响草原生态系统CO_2交换目前为止还没有定论。同时,不同类型和剂量氮素对生态系统CO_2交换影响的差异也不明确。选取内蒙古额尔古纳草甸草原,开展了不同类型氮肥和不同剂量氮素添加条件下生...氮沉降增加将影响草原生态系统固碳,但如何影响草原生态系统CO_2交换目前为止还没有定论。同时,不同类型和剂量氮素对生态系统CO_2交换影响的差异也不明确。选取内蒙古额尔古纳草甸草原,开展了不同类型氮肥和不同剂量氮素添加条件下生态系统CO_2交换的野外测定。实验设置尿素和缓释尿素2种类型氮肥各5个剂量水平(0、5.0、10.0、20.0和50.0g N·m^(–2)·a^(–1))。结果显示,生长季初期及中期降雨量低时,氮素添加抑制生态系统CO_2交换;而生长季末期降雨量较高时促进生态系统CO_2交换。随着氮素添加水平的提高,NEE和GEP均显著增加,当氮素添加量达到10 g N·m^(–2)·a^(–1)时,NEE和GEP的响应趋于饱和。2种氮肥(尿素和缓释尿素)仅在施氮量为5 g N·m^(–2)·a^(–1)时,缓释尿素对生态系统CO_2交换的促进作用显著大于尿素,在其它添加剂量时差异不显著。研究结果表明:氮素是该草甸草原生态系统的重要限制因子,但氮沉降增加对生态系统CO_2交换的影响强烈地受降雨量与降雨季节分配的限制,不同氮肥(尿素和缓释尿素)对生态系统CO_2交换作用存在差异。展开更多
The accurate measurement of the dynamics of photosynthesis in China’s subtropical evergreen forest ecosystems is an important contribution to carbon(C) sink estimates in global terrestrial ecosystems and their respon...The accurate measurement of the dynamics of photosynthesis in China’s subtropical evergreen forest ecosystems is an important contribution to carbon(C) sink estimates in global terrestrial ecosystems and their responses to climate change. Eddy covariance has historically been the only direct method to assess C flux of whole ecosystems with high temporal resolution, but it suffers from limited spatial resolution. During the last decade, continuous global monitoring of plant primary productivity from spectroradiometer sensors on flux towers and satellites has extended the temporal and spatial coverage of C flux observations. In this study, we evaluated the performance of two physiological remote sensing indices, fluorescence reflectance index(FRI) and photochemical reflectance index(PRI), to measure the seasonal variations of photosynthesis in a subtropical evergreen forest ecosystem using continuous canopy spectral and flux measurements in the Dinghushan Nature Reserve in southern China.The more commonly used NDVI has been shown to be saturated and mainly affected by illumination(R^2=0.88, p <0.001), but FRI and PRI could better track the seasonal dynamics of plant photosynthetic functioning by comparison and are less affected by illumination(R^2=0.13 and R^2=0.51, respectively) at the seasonal scale. FRI correlated better with daily gross primary production(GPP) in the morning hours than in the afternoon hours, in contrast to PRI which correlated better with light-use efficiency(LUE) in the afternoon hours. Both FRI and PRI could show greater correlations with GPP and LUE respectively in the senescence season than in the recovery-growth season. When incident PAR was taken into account, the relationship between GPP and FRI was improved and the correlation coefficient increased from 0.22 to 0.69(p < 0.001). The strength of the correlation increased significantly in the senescence season(R^2=0.79, p < 0.001). Our results demonstrate the application of FRI and PRI as physiological indices for the accurate measurement of the seasonal dynamics of plant community photosynthesis in a subtropical evergreen forest, and suggest these indices may be applied to carbon cycle models to improve the estimation of regional carbon budgets.展开更多
This study investigated the photosynthetic rate of the lichen Endocarpon pusillum at the Chinese Academy of Sciences Shapotou Desert Research Station and estimated its annual contribution to the carbon budget in the e...This study investigated the photosynthetic rate of the lichen Endocarpon pusillum at the Chinese Academy of Sciences Shapotou Desert Research Station and estimated its annual contribution to the carbon budget in the ecosystem. The software SigmaPlot 10.0 with "Macro-Area below curves" was used to calculate the carbon fixation capacity of the lichen. The total carbon budget (ΣC) of the lichen was obtained by subtracting the respiratory carbon loss (ΣDR) from the photosynthetic carbon gain (ΣNP). Because water from precipitation plays an important role in photosynthesis in this ecosystem, the annual carbon budget of E. pusillum at the station was estimated based on the three-year average precipitation data from 2009 to 2011. Our results indicate that the lichen fixes 14.6 g Cm-2 annually. The results suggest that artificial inoculation of the crust lichen in the Tengger Desert could not only help reduce the sand and dust storms but also offer a significant carbon sink, fixing a total of 438000 t of carbon over the 30000 km2 of the Tengger Desert. The carbon sink could potentially help mitigate the atmospheric greenhouse effect. Our study suggests that the carpet-like lichen E. pusillum is an excellent candidate for "Bio-carpet Engineering" of arid and semi-arid regions.展开更多
基金Supported by the National Natural Science Foundation of China(31060045,31260091)~~
文摘[Objective] The aim of this study was to investigate the content changes and their correlations of the photosynthetic pigment,phenols,including total phenols,total flavonoids and anthocyanins,and total saponins of the one-year-old P.notoginseng plants under supplemental UV-B stress in fields.[Method] The one-year-old plants were irradiated by UV-B in field for 1 min per day,and the plants under the UV-B lamp were regarded as a circle center,achieving an annular leaf-sampling.The photosynthetic pigment,phenols and total saponins of the leaves were determined spectrophotometrically.[Result] With the increase of sampling radius,the supplemental UV-B intensity decreased significantly,the contents of chlorophyll (Chl) a,Chl b,Chl (a+b),carotenoid (Car) and total photosynthetic pigment (Chl+Car) of the leaves increased extremely significantly,the Chl a/b and total phenol content (TPC) decreased extremely significantly,but the Chl (a+b)/Car changes were not significant.The contents of total flavonoids,anthocyanins and saponins all increased due to the increasing of UV-B,displaying dose effects.The UV-B intensity was positively correlated with the Chl a/b,and negatively with the Chl a,Chl b,Chl (a+ b),Car and (Chl+Car) contents; and the two of TPC,total flavonoid content (TFC),total anthocyanin content (TAC) and total saponin content (TSC) were positively correlated,all reaching extremely significant level.The UV-B intensity was positively and significantly correlated with the total flavonoid content (TFC),negatively and significantly with the Chl (a+b)/Car,and positively and insignificantly with the TPC,TAC and TSC.[Conclusion] For one-year-old plants of P.notoginseng,UV-B can decrease the contents of the Chl a,Chl b,Chl (a+b),Car and (Chl+Car) and increase the Chl a/b and TPC,and,furthermore,induce the increases of the TFC,TAC and TSC in a dose-dependent manner.However,UV-B can hardly change the Chl (a+b)/Car.The supplemental UV-B of well-suited dose might be one of the effective measures to improve the TSC of P.notoginseng.
基金Supported by the Knowledge Innovation Program of Chinese Academy of Sciences(No.SQ201213)the National Natural Science Foundation of China(No.31402320)+1 种基金the Guangdong Province and Chinese Academy of Science Cooperation Foundation(No.2012B091100268)the Technology Project of Ocean and Fisheries of Guangdong Province(No.A201101I04)
文摘Phaeodactylum tricornutum is a potential livestock for the combined production of eicosapentaenoic acid(EPA) and fucoxanthin. In this study, six marine diatom strains identified as P. tricornutum were cultured and their total lipid, fatty acid composition and major photosynthetic pigments determined. It was found that the cell dry weight concentration and mean growth rate ranged between 0.24–0.36 g/L and 0.31–0.33/d, respectively. Among the strains, SCSIO771 presented the highest total lipid content, followed by SCSIO828, and the prominent fatty acids in all strains were C16:0, C16:1, C18:1, and C20:5(EPA). Polyunsaturated fatty acids, including C16:2, C18:2, and EPA, comprised a significant proportion of the total fatty acids. EPA was markedly high in all strains, with the highest in SCSIO828 at 25.65% of total fatty acids. Fucoxanthin was the most abundant pigment in all strains, with the highest in SCSIO828 as well, at 5.50 mg/g. The collective results suggested that strain SCSIO828 could be considered a good candidate for the concurrent production of EPA and fucoxanthin.
文摘氮沉降增加将影响草原生态系统固碳,但如何影响草原生态系统CO_2交换目前为止还没有定论。同时,不同类型和剂量氮素对生态系统CO_2交换影响的差异也不明确。选取内蒙古额尔古纳草甸草原,开展了不同类型氮肥和不同剂量氮素添加条件下生态系统CO_2交换的野外测定。实验设置尿素和缓释尿素2种类型氮肥各5个剂量水平(0、5.0、10.0、20.0和50.0g N·m^(–2)·a^(–1))。结果显示,生长季初期及中期降雨量低时,氮素添加抑制生态系统CO_2交换;而生长季末期降雨量较高时促进生态系统CO_2交换。随着氮素添加水平的提高,NEE和GEP均显著增加,当氮素添加量达到10 g N·m^(–2)·a^(–1)时,NEE和GEP的响应趋于饱和。2种氮肥(尿素和缓释尿素)仅在施氮量为5 g N·m^(–2)·a^(–1)时,缓释尿素对生态系统CO_2交换的促进作用显著大于尿素,在其它添加剂量时差异不显著。研究结果表明:氮素是该草甸草原生态系统的重要限制因子,但氮沉降增加对生态系统CO_2交换的影响强烈地受降雨量与降雨季节分配的限制,不同氮肥(尿素和缓释尿素)对生态系统CO_2交换作用存在差异。
基金National Key Research and Development Program of China(2017YFC0503803)National Natural Science Foundation of China(41571192)+1 种基金Natural Science Foundation of Hebei,China(D2016302002)Science and Technology Planning Project of Hebei,China(17390313D)
文摘The accurate measurement of the dynamics of photosynthesis in China’s subtropical evergreen forest ecosystems is an important contribution to carbon(C) sink estimates in global terrestrial ecosystems and their responses to climate change. Eddy covariance has historically been the only direct method to assess C flux of whole ecosystems with high temporal resolution, but it suffers from limited spatial resolution. During the last decade, continuous global monitoring of plant primary productivity from spectroradiometer sensors on flux towers and satellites has extended the temporal and spatial coverage of C flux observations. In this study, we evaluated the performance of two physiological remote sensing indices, fluorescence reflectance index(FRI) and photochemical reflectance index(PRI), to measure the seasonal variations of photosynthesis in a subtropical evergreen forest ecosystem using continuous canopy spectral and flux measurements in the Dinghushan Nature Reserve in southern China.The more commonly used NDVI has been shown to be saturated and mainly affected by illumination(R^2=0.88, p <0.001), but FRI and PRI could better track the seasonal dynamics of plant photosynthetic functioning by comparison and are less affected by illumination(R^2=0.13 and R^2=0.51, respectively) at the seasonal scale. FRI correlated better with daily gross primary production(GPP) in the morning hours than in the afternoon hours, in contrast to PRI which correlated better with light-use efficiency(LUE) in the afternoon hours. Both FRI and PRI could show greater correlations with GPP and LUE respectively in the senescence season than in the recovery-growth season. When incident PAR was taken into account, the relationship between GPP and FRI was improved and the correlation coefficient increased from 0.22 to 0.69(p < 0.001). The strength of the correlation increased significantly in the senescence season(R^2=0.79, p < 0.001). Our results demonstrate the application of FRI and PRI as physiological indices for the accurate measurement of the seasonal dynamics of plant community photosynthesis in a subtropical evergreen forest, and suggest these indices may be applied to carbon cycle models to improve the estimation of regional carbon budgets.
基金supported by the National Natural Science Foundation of China (31070018)the Knowledge Innovation Program of the Chinese Academy of Sciences (2010-Biols-CAS-0104)Ministry of Science and Technology of China (2011BAC07B03)
文摘This study investigated the photosynthetic rate of the lichen Endocarpon pusillum at the Chinese Academy of Sciences Shapotou Desert Research Station and estimated its annual contribution to the carbon budget in the ecosystem. The software SigmaPlot 10.0 with "Macro-Area below curves" was used to calculate the carbon fixation capacity of the lichen. The total carbon budget (ΣC) of the lichen was obtained by subtracting the respiratory carbon loss (ΣDR) from the photosynthetic carbon gain (ΣNP). Because water from precipitation plays an important role in photosynthesis in this ecosystem, the annual carbon budget of E. pusillum at the station was estimated based on the three-year average precipitation data from 2009 to 2011. Our results indicate that the lichen fixes 14.6 g Cm-2 annually. The results suggest that artificial inoculation of the crust lichen in the Tengger Desert could not only help reduce the sand and dust storms but also offer a significant carbon sink, fixing a total of 438000 t of carbon over the 30000 km2 of the Tengger Desert. The carbon sink could potentially help mitigate the atmospheric greenhouse effect. Our study suggests that the carpet-like lichen E. pusillum is an excellent candidate for "Bio-carpet Engineering" of arid and semi-arid regions.