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总枝状毛霉对去氢表雄酮的生物转化研究 被引量:5
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作者 葛文中 李楠 +2 位作者 单丽红 王彦杰 刘宏民 《微生物学杂志》 CAS CSCD 2007年第1期14-17,共4页
从土样中筛选到1株能转化甾体的菌株,经形态观察,鉴定为总枝状毛霉(Mucor racemosus)。利用该菌株对去氢表雄酮进行生物转化研究,转化产物分离后,通过红外、质谱和二维核磁波谱分析,确定了化学结构。研究结果表明转化产物是7α-羟基去... 从土样中筛选到1株能转化甾体的菌株,经形态观察,鉴定为总枝状毛霉(Mucor racemosus)。利用该菌株对去氢表雄酮进行生物转化研究,转化产物分离后,通过红外、质谱和二维核磁波谱分析,确定了化学结构。研究结果表明转化产物是7α-羟基去氢表雄酮和7β-羟基去氢表雄酮。 展开更多
关键词 生物转化 总枝状毛霉 7α羟基化 去氢表雄酮 甾体
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用微生物转化法合成4-孕甾-3,20-二酮的羟基类衍生物
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作者 李合平 黄利华 +2 位作者 单丽红 刘宏民 葛文中 《郑州大学学报(医学版)》 CAS 北大核心 2006年第2期256-257,共2页
目的合成具有潜在活性的4-孕甾-3,20-二酮的羟基类衍生物。方法和结果利用本中心筛选的总枝状毛霉(Macorracemosus)进行微生物转化,得到的产物经熔点、红外、核磁和质谱分析,确定为预期产物14β-羟基-孕甾-3,20-二酮和7β,14β二羟基-孕... 目的合成具有潜在活性的4-孕甾-3,20-二酮的羟基类衍生物。方法和结果利用本中心筛选的总枝状毛霉(Macorracemosus)进行微生物转化,得到的产物经熔点、红外、核磁和质谱分析,确定为预期产物14β-羟基-孕甾-3,20-二酮和7β,14β二羟基-孕甾-3,20-二酮。结论用微生物转化法可以制备甾体的羟基类衍生物。 展开更多
关键词 4-孕甾-3 20-二酮 总枝状毛霉 生物转化
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Isolation of Mucor circinelloides Z4 and Mucor racemosus Z8 from heavy metal-contaminated soil and their potential in promoting phytoextraction with Guizhou oilseed rap 被引量:1
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作者 朱生翠 汤建新 +3 位作者 曾晓希 魏本杰 杨少迪 黄斌 《Journal of Central South University》 SCIE EI CAS CSCD 2015年第1期88-94,共7页
Fungi Z4 and Z8, isolated from the heavy metal polluted soil, have strong resistance to Cd and Pb. The strains were identified on the base of their morphology and internal transcribed spacers(ITS) region. Pot experime... Fungi Z4 and Z8, isolated from the heavy metal polluted soil, have strong resistance to Cd and Pb. The strains were identified on the base of their morphology and internal transcribed spacers(ITS) region. Pot experiments were conducted to study the effect of two strains(Z4 and Z8) on the growth and accumulation of Cd and Pb of Guizhou oilseed rape. The results show that strains Z4 and Z8 belong to Mucor circinelloides and Mucor racemosus, respectively. The heights of Guizhou oilseed rape inoculated with strain Z8 increase by 47.90% than the control. The highest fresh mass is found in the plant with Z4/Z8, which is enhanced by160.81%. Pot experiments show that Z4/Z8 inoculums can accelerate accumulation of heavy metals in the plant. The contents of Cd and Pb are increased by 117.60% and 63.48%, respectively. Meanwhile, the heavy metal concentrations in potting soil with the two strains are found to be lower than those of the control, and the concentrations of Cd and Pb are decreased by 60.57% and 27.12%,respectively. 展开更多
关键词 phytoextraction fungus cadmium lead oilseed rape
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