Fabrication of a novel electrochemical sensor based on MnFe_(2)O_(4)/graphene modified glassy carbon electrode for the sensitive detection of bisphenol A

Manganese ferrite(MnFe_(2)O_(4))has the advantages of simple preparation,high resistivity,and high crystal symmetry.Herein,we have developed an electrochemical sensor utilizing graphene and MnFe_(2)O_(4) nanocomposites modified glassy carbon electrode(GCE),which is very efficient and sensitive to detect bisphenol A(BPA).MnFe_(2)O_(4)/graphene(GR)was synthesized by immobilizing the MnFe_(2)O_(4) microspheres on the graphene nanosheets via a simple one-pot solvothermal method.The morphology and structure of the MnFe_(2)O_(4)/GR nanocomposite have been characterized through scanning electron microscopy(SEM),Fourier transform infrared spectroscopy(FT-IR),X-ray diffraction(XRD)and X-ray photoelectron spectroscopy(XPS).In addition,electrochemical properties of the modified materials are comparably explored by means of cyclic voltammetry(CV),electrochemical impedance spectroscopy(EIS)and differential pulse voltammetry(DPV).Under the optimal conditions,the proposed electrochemical sensor for the detection of BPA has a linear range of 0.8-400μmol/L and a detection limit of 0.0235μmol/L(S/N=3)with high sensitivity,good selectivity and high stability.In addition,the proposed sensor was used to measure the content of BPA in real water samples with a recovery rate of 97.94%-104.56%.At present,the synthesis of MnFe_(2)O_(4)/GR provides more opportunities for the electrochemical detection of BPA in practical applications.

寄主的物理和化学因子对马尾松毛虫产卵行为的作用

实验表明,交尾后马尾松毛虫雌虫产卵时对寄主的选择行为受基质的形状和化学组成的影响,但看来化学因子更为重要。生测表明,活性物质溶于石油醚、乙醚、乙醇和水,并且可用水蒸馏法蒸出。这些物质主要分布于松针的表面。被马尾松毛虫取食过的松针其相应抽提物则失去了活性。气相色谱分析显示,取食过的松针抽提物含有正常松针抽提物所具有的全部化学成分,但有些物质的含量却变化了,并且出现了新的化合物。用切除手术实验证明雌虫接受寄主影响雌虫产卵行为的化学物质的感化器主要分布在其中垫上。

Computational analysis of genetic loci required for amphid structure and functions and their possibly corresponding microRNAs in C. elegans

Objective To examine the important roles of microRNAs （miRNAs） in regulating amphid structure and function, we performed a computational analysis for the genetic loci required for the sensory perception and their possibly corresponding miRNAs in C. elegans. Methods Total 55 genetic loci required for the amphid structure and function were selected. Sequence alignment was combined with E value evaluation to investigate and identify the possible corresponding miRNAs. Results Total 30 genes among the 55 genetic loci selected have their possible corresponding regulatory miRNA（s）, and identified genes participate in the regulation of almost all aspects of amphid structure and function. In addition, our data suggest that both the amphid structure and the amphid functions might be regulated by a series of network signaling pathways. Moreover, the distribution of miRNAs along the 3＇ untranslated region （UTR） of these 30 genes exhibits different patterns. Conclusion We present the possible miRNA-mediated signaling pathways involved in the regulation of chemosensation and thermosensation by controlling the corresponding sensory neuron and interneuron functions. Our work will be useful for better understanding of the miRNA-mediated control of the chemotaxis and thermotaxis in C. elegans.

Electrocatalytic Oxidation of Saccharides at MoOx/AuNPs Modified Electrode Towards Analytical Application

The MoOx/AuNPs composite film modified glassy carbon electrode was fabricated by electro-depositing simultaneously gold nanoparticles and molybdenum oxides using cyclic voltammetry. The morphology and topography of the MoOx/AuNPs composite were char-acterized by scan electron microscopy and X-ray photoelectron spectroscopy respectively, and the electrocatalytic oxidation of glucose at the MoOx/AuNPs composite film was inves-tigated and analyzed in detail. It was shown that the MoOx/AuNPs composite was of strong electrocatalytic activity towards oxidation of glucose as well as other saccharides, so that an attempt was made for direct voltammetric determination of glucose. Then the positive scan polarization reverse catalytic voltammetry was proposed for the first time. Based on this method, the pure oxidation current was extracted by subtraction of the blank current in the reverse scan. The current sensitivity was enhanced tremendously and the signal to noise ra-tio was improved adequately. The electrocatalytic oxidation of glucose at the MoOx/AuNPs modified electrode was performed in alkaline medium, a wide linear range from 0.01 mmol/L to 4.0 mmol/L of glucose, a higher current sensitivity of 2.35 mA/（mmol/L·cm2）, and a lower limit of detection of 9.01 μmol/L （at signal/noise=3） were achieved. In addition, the electrocatalytic oxidation of other saccharides such as lactose, fructose and sucrose was also evaluated.

5Gb/s 0.25μm CMOS Limiting Amplifier

A limiting amplifier (LA) IC implemented in TSMC standard 0.25μm CMOS technology is described.Active inductor loads and direct-coupled technology are employed to increase the gain,broaden the bandwidth,reduce the power dissipation,and keep a tolerable noise performance.Under a 3.3V supply voltage,the LA core achieves a gain of 50-dB with a power consumption below 40mW.The measured input sensitivity of the amplifier is better than 5m V _ pp .It can operate at bit rates up to 7Gb/s with an rms jitter of 0.03 UI or less.The chip area is only 0.70mm×0.70mm.According to the measurement results,this IC is expected to work at the standard bit rate levels of 2.5,3.125,and 5Gb/s.

PLASMA RESONANCE FIBER OPTIC SENSOR FOR CURE MONITORING OF EPOXY COMPOSITE

The plasma resonance fiber optic sensor has a research values in theory and is widely used in engineering because of its simple structure and high sensitivity. It is a simple and sensitive method to measure the refractive index with optical fiber plasma wave. We make use of this characteristic to manufacture the plasma resonance fiber optic sensor which can detect the cure of epoxy compo site. We study the method of testing the solutions which have different refractive index with plasma resonance fiber optic sensor. A fiber optic sensing probe which has reliable performance and convenient operation for detecting the refractive index has been designed. The system for detecting the solution refractive index is developed and used to measure the refractive index of epoxy during the different phases in the cure process. Result shows that this system is credible and stable, the parameters tested are in accord with the facts.

Sensors layout optimization in explosion overpressure field reconstruction

The paper proposes four indicators to guide sensors layout in practical experiment on explosion overpressure filed construction based on tomographic method with high reconstruction accuracy and the least sensors. First, genetic algorithm is adopted to conduct global search and sensor layout optimization method is selected to satisfy four indicators. Then, by means of Matlab, the variation of these four indicators with different sensor layouts and reconstruction accuracy are analyzed and discussed. The results indicate that the sensor layout method proposed by this paper can reconstruct explosion overpressure field at the highest precision by a minimum number of sensors. It will guide actual explosion experiments in a cost-effective way.

Cyclin Dl antisense oligodexoyneucleotides inhibits growth and enhances chemosensitivity in gastric carcinoma cells

AIM： To examine the effects of cyclin D1 antisense oligodexoyneucleotides （ASODN） on growth and chemosensitivity of gastric carcinoma cell lines SGC7901 and its mechanism. METHODS： Phosphorothioate modified cyclin D1 ASODN was encapsulated by LipofectAMINE2000 （LF2000） and transfected into cells, the dose-effect curves and growth curves were observed. 5-FU, MTX, CDDP of different concentrations were given after transfecting cells with cyclin D1 ASODN for 24 h, the dose-effect responses were observed and IC50s were calculated. The mRNA expression of cyclin D1, thymidylate synthase （TS）, thymidine phosphorylase （TP） and dihydrofolate reductase （DHFR） was detected by reverse transcription-PCR （RTPCR） at 24 h and 48 h after transfection. RESULTS： Dose-dependent inhibitory effect was caused by cyclin D1 ASODN in SGC7901 cells. Transfecting gastric carcinoma cells with 0.2 μmol/L cyclin D1 ASODN for 24 h could inhibit growth significantly and reduce expression of cyclin D1 mRNA. Cyclin D1 ASODN could increase the chemosensitivity to 5-FU, MTX, CDDP in cells, The IC50s of different chemotherapeutic agents in ASODN plus chemotherapy groups were significantly lower than those in controls. Transfection with cyclin D1 ASODN leaded to an increase in TS and DHFR mRNA and a decrease in TP mRNA as determined by RT-PCR at 24 h, the alterations were more significant at 48 h. CONCLUSIONS： Cyclin D1 ASODN can decrease mRNA expression of cyclin D1, inhibit growth and enhance the chemosensitivity by changing the expression of enzymes related to metabolism of chemotherapeutic agents in SGC7901 gastric carcinoma cells.

Securing Sensor Networks Based on Optimization of Weighted Confidence

In this paper, an optimized rmlicious nodes detection algorithm, based on Weighted Confidence Filter （WCF）, is proposed to protect sensor networks from attacks. In this algorithm, each cluster head in a cluster-based hierarchical network figures out an average confidence degree by means of messages from its child nodes. The cluster head only accepts a message from the child node whose confidence degree is higher than the average. Meanwhile, it updates the confidence degrees for each of its child nodes by comparing the aggregation value and the received messages, and regards them as the weight of exactness of messages from nodes. A sensor node is judged to be rmlicious if its weight value is lower than the predefined threshold. Comparative simulation results verify that the proposed WCF algorithm is better than the Weighted Trust Evaluation （WTE） in terms of the detection ratio and the false alarm ratio. More specifically, with the WCF, the detection ratio is significantly improved and the false alarm ratio is observably reduced, especially when the malicious node ratio is 0.25 or greater. When 40% of 100 sensors are malicious, the detection accuracy is above 90% and the false alarm ratio is nearly only 1.8%.

Mobile robot localization algorithm based on multi-sensor information fusion

In order to effectively reduce the uncertainty error of mobile robot localization with a single sensor and improve the accuracy and robustness of robot localization and mapping,a mobile robot localization algorithm based on multi-sensor information fusion(MSIF)was proposed.In this paper,simultaneous localization and mapping(SLAM)was realized on the basis of laser Rao-Blackwellized particle filter(RBPF)-SLAM algorithm and graph-based optimization theory was used to constrain and optimize the pose estimation results of Monte Carlo localization.The feature point extraction and quadrilateral closed loop matching algorithm based on oriented FAST and rotated BRIEF(ORB)were improved aiming at the problems of generous calculation and low tracking accuracy in visual information processing by means of the three-dimensional(3D)point feature in binocular visual reconstruction environment.Factor graph model was used for the information fusion under the maximum posterior probability criterion for laser RBPF-SLAM localization and binocular visual localization.The results of simulation and experiment indicate that localization accuracy of the above-mentioned method is higher than that of traditional RBPF-SLAM algorithm and general improved algorithms,and the effectiveness and usefulness of the proposed method are verified.

A NEW BIOGENIC SULFIDE CHEMICAL SENSOR FOR MARINE ENVIRONMENTAL MONITORING AND SURVEY

A new convenient sulfide electrochemical sensor for marine environmental in situ monitoring and real time survey was developed. The new sensor based on a solid Ag 2S membrane electrode has outstanding chemical sensitivity and stability. It responds to the activity of sulfide ions according to a Nernstian slope of -31mV/decade. The sensor can be used to determine the total concentration of sulfides ( C T) by calibrating the pH value of the solution to a standard pH. The practical measurement range for total sulfide concentration is 0.1-10 mg/L in seawater. The sensor has a very low potential drift (<4mV) during two months in 0.1 mg/L sulfide seawater. This paper describes the preparation of the sensitive membrane and some main properties of the sensor.

Three-dimensionally ordered macroporous perovskite materials for environmental applications

Three-dimensionally ordered macroporous(3DOM)perovskite materials have attracted the interest from researchers worldwide due to their unique macroporous structure,flexible composition,tailorable physicochemical property,high stability and biocompatibility.In particular,they were widely used in environmental field,such as photocatalysis,catalytic combustion,catalytic oxidation and sensors.In this review,the recent progresses in the synthesis of 3DOM perovskite materials and their environmental applications are summarized.The advantages and the promoting mechanisms of 3DOM perovskite materials for different applications are discussed in detail.Subsequently,the challenges and perspectives on the topic are proposed.

Involvement of autophagy in alcoholic liver injury and hepatitis C pathogenesis

This review describes the principal pathways of macroautophagy (i.e. autophagy), microautophagy and chaperone-mediated autophagy as they are currently known to occur in mammalian cells. Because of its crucial role as an accessory digestive organ, the liver has a particularly robust autophagic activity that is sensitive to changes in plasma and dietary components. Ethanol consumption causes major changes in hepatic protein and lipid metabolism and both are regulated by autophagy, which is significantly affected by hepatic ethanol metabolism. Ethanol exposure enhances autophagosome formation in liver cells, but suppresses lysosome function. Excessive ethanol consumption synergizes with hepatitis C virus (HCV) to exacerbate liver injury, as alcohol-consuming HCV patients frequently have a longer course of infection and more severe manifestations of chronic hepatitis than abstinent HCV patients. Alcohol-elicited exacerbation of HCV infection pathogenesis is related to modulation by ethanol metabolism of HCV replication. Additionally, as part of this mechanism, autophagic proteins have been shown to regulate viral (HCV) replication and their intracel-lular accumulation. Because ethanol induces autophagosome expression, enhanced levels of autophagic proteins may enhance HCV infectivity in liver cells of alcoholics and heavy drinkers.

The performance of nano multicomponent cocatalyst in the improvement of catalytic sensor of methane

Based on the deficiency of catalytic elements in methane sensors such as sintering,activity decrease and surface area reduction at high temperature, three differentnano vectors Ce-Zr-Al_2O_3, Ce-Al_2O_3, and Zr-Al_2O_3 were prepared via sol-gel technique inthe experiment.BET surface area, catalytic activity and thermal stability were tested andcompared.It is found from the experiment that the Ce-doped Al_2O_3 vector possesseshigher catalytic activity than pure Al_2O_3 vector.Zr-doped Al_2O_3 vector can enhance thethermal stability of methane sensors.Ce-Zr-Al solid solution can be obtained by the presenceof Ce and Zr doped with Al_2O_3.The reaction activity and thermal stability of catalyticsensors were improved because of the unique synergy effect from Ce-Zr-O.Among themixed cocatalysts, Ce-Zr-O was reported to be an excellent cocatalyst material.The performanceof methane sensors can be improved significantly via the modification ofCe-Zr-Al_2O_3 vector.

Fluorescence Enhancement of Polyamine Derivatives of 1,8-Naphthalimide with Transition Metal Ions

A series of fluorescent chemosensors 1-3 were synthesized to detect transition metal ions. At the room temperature, fluorescence intensities of these chemosensors in acetonitrile without transition metal ions were found to be very weak, due to the process of the efficient intramolecular photoinduced electron transfer （PET）. However, after addition of the transition metal ions, the chemoscnsor 1-3 exhibits obvious fluorescence enhancement. Moreover, the intensity of the fluorescence emission of chemosensors increases significantly in the presence of Zn^2＋ and Cd^2＋. The fluorescent chemosensors with different polyamine as receptors show diverse affinity abilities to the transition metal ions and signal the receptor-metal ion interaction by the intensity change of fluorescence emission.

COLLABORATIVE TRACKING VIA PARTICLE FILTER IN WIRELESS SENSOR NETWORKS

Target tracking is one of the main applications of wireless sensor networks. Optimized computation and energy dissipation are critical requirements to save the limited resource of the sensor nodes. A framework and analysis for collaborative tracking via particle filter are presented in this paper. Collaborative tracking is implemented through sensor selection, and results of tracking are propagated among sensor nodes. In order to save communication resources, a new Gaussian sum particle filter, called Gaussian sum quasi particle filter, to perform the target tracking is presented, in which only mean and covariance of mixands need to be communicated. Based on the Gaussian sum quasi particle filter, a sensor selection criterion is proposed, which is computationally much simpler than other sensor selection criterions. Simulation results show that the proposed method works well for target tracking.

New amperometric glucose biosensor by entrapping glucose oxidase into chitosan/nanoporous ZrO_2/multiwalled carbon nanotubes nanocomposite film

A new nanocomposite material for construction of glucose biosensor was prepared. The biosensor was formed by entrapping glucose oxidase(Gox) into chitosan/nanoporous ZrO2/multiwalled carbon nanotubes nanocomposite film. In this biosensing thin film, the multiwalled carbon nanotubes can effectively catalyze hydrogen peroxide and nanoporous ZrO2 can enhance the stability of the immobilized enzyme. The resulting biosensor provides a very effective matrix for the immobilization of glucose oxidase and exhibits a wide linear response range from 8 μmol/L to 3 mmol/L with a correlation coefficient of 0.994 for the detection of glucose. And the response time and detection limit of the biosensor are determined to be 6 s and 3.5 μmol/L, respectively. Another attractive characteristic is that the biosensor is inexpensive, stable and reliable.

Fuzzy Neural Network Model of 4-CBA Concentration for Industrial Purified Terephthalic Acid Oxidation Process

A fuzzy neural network (FNN) model is developed to predict the 4-CBA concentration of the oxidation unit in purified terephthalic acid process. Several technologies are used to deal with the process data before modeling.First,a set of preliminary input variables is selected according to prior knowledge and experience. Secondly,a method based on the maximum correlation coefficient is proposed to detect the dead time between the process variables and response variables. Finally, the fuzzy curve method is used to reduce the unimportant input variables.The simulation results based on industrial data show that the relative error range of the FNN model is narrower than that of the American Oil Company (AMOCO) model. Furthermore, the FNN model can predict the trend of the 4-CBA concentration more accurately.

Study of a high-temperature and high-pressure FBG sensor with Al_2O_3 thin-wall tube substrate

A fiber Bragg grating(FBG) high-temperature and high pressure sensor has been designed and fabricated by using the Al2O3 thin-wall tube as a substrate.The test results show that the sensor can withstand a pressure range of 0-45 MPa and a temperature range of -10-300 ℃,and has a pressure sensitivity of 0.0426 nm/MPa and a temperature sensitivity of 0.0112 nm /

Alumina Photonic Crystals with Defect Modes for Sensor Application

One-dimensional alumina photonic crystals with defect modes were successfully fabricated through inserting a constant voltage waveform into the periodic voltage signals. The trans-mission spectra show that the thickness of defects plays a key role in determining the trans-mittance of defect modes. When the thickness was ？180 nm, an obvious defect mode with the high transmittance of 55% and a narrow full width at half maximum of 18 nm was observed in the original photonic band gaps. The defect mode shifted linearly with the increasing of refractive index of the analytes infiltrated into pores, indicating its potential application in chemical sensing or bio-sensing.