颈动脉体瘤（carotid body tumor,CBT）是一种临床较为少见、来源于颈动脉体化学感受器的肿瘤[1]。虽然放射治疗对CBT有一定的效果[2],但手术仍是目前治疗CBT的唯一有效方法[3]。理想的手术方式是将瘤体完整地从颈动脉壁上剥离并保全颈...颈动脉体瘤（carotid body tumor,CBT）是一种临床较为少见、来源于颈动脉体化学感受器的肿瘤[1]。虽然放射治疗对CBT有一定的效果[2],但手术仍是目前治疗CBT的唯一有效方法[3]。理想的手术方式是将瘤体完整地从颈动脉壁上剥离并保全颈动脉的连续性和完整性。对于肿瘤体积较大、瘤体与动脉壁粘连紧密的CBT,手术单纯剥离较为困难,往往需要切除颈动脉并行血管重建[4-5]。2009-2014年我科共手术治疗29例CBT患者,均成功切除了肿瘤并保全了颈动脉的连续性和完整性,取得了较好效果,现报告如下。展开更多
AIM: To determine how glucocorticoids (GCs) may affect the growth and chemosensitivity of common carcinoma cells.METHODS: The effect of dexamethasone (DEX) on growth and chemosensitivity was assessed in 14 carcinoma c...AIM: To determine how glucocorticoids (GCs) may affect the growth and chemosensitivity of common carcinoma cells.METHODS: The effect of dexamethasone (DEX) on growth and chemosensitivity was assessed in 14 carcinoma cell lines. The function of GC receptors (GR) was assessed by MMTV reporter assay. Overexpression of GR was done by in vitro transfection and expression of a GR-expressing vector. Immunohistochemical stain of tissues and cells were done by PA1-511A, an anti-GR monoclonal antibody.RESULTS: DEX inhibited cell growth of four (MCF-7, MCF-7/MXR1, MCF-7/TPT300, and HeLa), increased cisplatin cytotoxicity of one (SiHa), and decreased cisplatin cytotoxicity of two (H460 and Hep3B) cell lines. The GR content of the seven cell lines affected by DEX was significantly higher than those of the seven cell lines unaffected by DEX(5.2±2.5×104 sites/cell vs 1.3±1.4×104 sites/cell, P= 0.005).Only two DEX-unresponsive cell lines (NPC-TW01 and NPCTW04) contained high GR amounts in the range (1.9-8.1×104sites/cell) of the seven DEX-responsive cell lines. The GR function of NPC-TW01 and NPC-TW04, however, was found to be impaired. The importance of high cellular amount of GR in mediating DEX susceptibility of the cells was further exemplified by GR dose-dependent drug resistance to cisplatin of AGS, a cell line with low GR content and was unaffected by DEX before transfection of GR-expressing vector. Immunohistochemical studies of human cancer tissues showed that 5 of the 45 (11.1%) breast cancer and 43 of the 85 (50.6%) non-small cell lung cancer had high GR contents at the ranges of the GC-responsive carcinoma cell lines.CONCLUSION: The growth and chemosensitivity of human carcinomas with high GR contents may be affected by GC. However, in light of the heterogeneous and even contradictive effects of GC on these cells, routine examination of GR contents of human carcinoma tissues may not be clinically useful until other markers that help predict the ultimate effect of GC on individual patients are identified.展开更多
基金National Natural Science Foundation of China(Nos.31520103915,31730090,and 31322053)the Hubei Provincial Natural Science Foundation of China(No.2018CFA020)
文摘颈动脉体瘤（carotid body tumor,CBT）是一种临床较为少见、来源于颈动脉体化学感受器的肿瘤[1]。虽然放射治疗对CBT有一定的效果[2],但手术仍是目前治疗CBT的唯一有效方法[3]。理想的手术方式是将瘤体完整地从颈动脉壁上剥离并保全颈动脉的连续性和完整性。对于肿瘤体积较大、瘤体与动脉壁粘连紧密的CBT,手术单纯剥离较为困难,往往需要切除颈动脉并行血管重建[4-5]。2009-2014年我科共手术治疗29例CBT患者,均成功切除了肿瘤并保全了颈动脉的连续性和完整性,取得了较好效果,现报告如下。
基金Supported by grants from the National Science Council No.NSC 93-2314-B-002-006, Taiwan, and grants from National Taiwan University Hospital 91-N006
文摘AIM: To determine how glucocorticoids (GCs) may affect the growth and chemosensitivity of common carcinoma cells.METHODS: The effect of dexamethasone (DEX) on growth and chemosensitivity was assessed in 14 carcinoma cell lines. The function of GC receptors (GR) was assessed by MMTV reporter assay. Overexpression of GR was done by in vitro transfection and expression of a GR-expressing vector. Immunohistochemical stain of tissues and cells were done by PA1-511A, an anti-GR monoclonal antibody.RESULTS: DEX inhibited cell growth of four (MCF-7, MCF-7/MXR1, MCF-7/TPT300, and HeLa), increased cisplatin cytotoxicity of one (SiHa), and decreased cisplatin cytotoxicity of two (H460 and Hep3B) cell lines. The GR content of the seven cell lines affected by DEX was significantly higher than those of the seven cell lines unaffected by DEX(5.2±2.5×104 sites/cell vs 1.3±1.4×104 sites/cell, P= 0.005).Only two DEX-unresponsive cell lines (NPC-TW01 and NPCTW04) contained high GR amounts in the range (1.9-8.1×104sites/cell) of the seven DEX-responsive cell lines. The GR function of NPC-TW01 and NPC-TW04, however, was found to be impaired. The importance of high cellular amount of GR in mediating DEX susceptibility of the cells was further exemplified by GR dose-dependent drug resistance to cisplatin of AGS, a cell line with low GR content and was unaffected by DEX before transfection of GR-expressing vector. Immunohistochemical studies of human cancer tissues showed that 5 of the 45 (11.1%) breast cancer and 43 of the 85 (50.6%) non-small cell lung cancer had high GR contents at the ranges of the GC-responsive carcinoma cell lines.CONCLUSION: The growth and chemosensitivity of human carcinomas with high GR contents may be affected by GC. However, in light of the heterogeneous and even contradictive effects of GC on these cells, routine examination of GR contents of human carcinoma tissues may not be clinically useful until other markers that help predict the ultimate effect of GC on individual patients are identified.
