Objectives: To determine the susceptibilities of M.hominis and U. urealyticum to fluoroquinolones forthe instruction of reasonable clinical application ofantibiotics.Method: The susceptibilities of M. hominis and U.ur...Objectives: To determine the susceptibilities of M.hominis and U. urealyticum to fluoroquinolones forthe instruction of reasonable clinical application ofantibiotics.Method: The susceptibilities of M. hominis and U.urealyticum to six fluoroquinolones were determinedby the broth dilution method.Results: Sparfloxacin and gatifloxacin were veryactive with MIC50S of 0.03125 and 0.25 μg/ml againstM. hominis, 0.25 and 0.5 μg/ml against U. urealyticum,respectively. Levofloxacin and ofloxacin had MIC50S of1 μg/ml and 2 μg/ml, respectively against both species.Norfloxacin was less effective against both species at16 and 32 μg/ml. Ciprofloxacin was unusual in thatthe MIC50S varied fourfold between species, with 2 μg/ml against M. hominis and 8 μg/ml against U.urealyticum.Conclusions: The results can provide useful infor-mation for selecting fluoroquinolones for treatmentof urogenital mycoplasma infections.展开更多
This paper presents a comparative visualization strategy of slope failure susceptibility maps for analyzing different types of simultaneous occurrences of slope failures. Through the SEM (structural equation modeling...This paper presents a comparative visualization strategy of slope failure susceptibility maps for analyzing different types of simultaneous occurrences of slope failures. Through the SEM (structural equation modeling), slope failure susceptibility maps are produced by using causal factors (i.e., geographical information, satellite remotely sensed data). As for a conventional pair-wise comparative procedure, the differences between susceptibility maps are delineated on difference maps, that can be, however, applied for evaluating differences only between pairs of susceptibility maps. One of the strong requirements from specialists working on slope stability evaluation is a comparative and visualization strategy of susceptibility maps with respect to "different types of simultaneous slope failures", for which the discussion is insufficient in the previous research activities for constructing the quantitative models for slope failure hazard mapping. As a measure, a color composite map based on susceptibility maps has been produced. The combination of assigning susceptibility maps to RGB-color planes is determined based on an index of "NCCT (normalized correlated color temperature)" which represents the relationship between chromaticity and human visual perception. Through the cases examined, the result indicates that the proposed color composite map, as a heuristic visualization strategy, is useful for simultaneously evaluating the hazardous areas affected by "different types of slope failures".展开更多
Influence factors of frozen soil blastability are analyzed which mainly conclude the strain energy coefficient, tensile strength, compressive strength, longitudinal wave velocity and transverse wave velocity. Accordin...Influence factors of frozen soil blastability are analyzed which mainly conclude the strain energy coefficient, tensile strength, compressive strength, longitudinal wave velocity and transverse wave velocity. According to the principle of perceptron neural network, at first the index factors are standardized by the aid of the efficient function theory, then the blastability of frozen sand at -7, -12 and -17 ℃ are classified three categories. Through adjusting the weight value and threshold value, we can obtain that the clay blastability at -7 ℃ is close to the sand blastability at -12 ℃, they belong to the second category, the clay blastability at -12 ℃ is close to the sand blastability at -17 ℃, thus they are divided into the third category.展开更多
Neutralizing antibodies are considered to be an important protective parameter used in HIV-1 vaccine evaluation. However, the exact role that neutralizing antibodies plays in controlling the disease progression of HIV...Neutralizing antibodies are considered to be an important protective parameter used in HIV-1 vaccine evaluation. However, the exact role that neutralizing antibodies plays in controlling the disease progression of HIV-1 infected peoples is still undetermined. In this paper, we compared the protective function of the neutralizing antibody response in the plasma from LTNP and TP against clade B and clade C pseudoviruses. No difference in the neutralizing activities between the plasma from LTNP and TP was found, which was consistent with the most recent reports. In addition, no correlations between the titer or breadth and CD4+ or viral load in HIV-1 infected individuals were found. The protective roles played by neutralizing antibodies in controlling disease progression of HIV-1 infected people need to be considered in a new viewpoint.展开更多
In underwater target detection, the bottom reverberation has some of the same properties as the target echo, which has a great impact on the performance. It is essential to study the difference between target echo and...In underwater target detection, the bottom reverberation has some of the same properties as the target echo, which has a great impact on the performance. It is essential to study the difference between target echo and reverberation. In this paper, based on the unique advantage of human listening ability on objects distinction, the Gammatone filter is taken as the auditory model. In addition, time-frequency perception features and auditory spectral features are extracted for active sonar target echo and bottom reverberation separation. The features of the experimental data have good concentration characteristics in the same class and have a large amount of differences between different classes, which shows that this method can effectively distinguish between the target echo and reverberation.展开更多
To Integrate the capacity of sensing, communication, computing, and actuating, one of the compelling technological advances of these years has been the appearance of distributed wireless sensor network (DSN) for infor...To Integrate the capacity of sensing, communication, computing, and actuating, one of the compelling technological advances of these years has been the appearance of distributed wireless sensor network (DSN) for information gathering tasks. In order to save the energy, multi-hop routing between the sensor nodes and the sink node is necessary because of limited resource. In addition, the unpredictable conditional factors make the sensor nodes unreliable. In this paper, the reliability of routing designed for sensor network and some dependability issues of DSN, such as MTTF (mean time to failure) and the probability of connectivity between the sensor nodes and the sink node are analyzed. Unfortunately, we could not obtain the accurate result for the arbitrary network topology, which is #P-hard problem. And the reliability analysis of restricted topologies clustering-based is given. The method proposed in this paper will show us a constructive idea about how to place energy-constrained sensor nodes in the network efficiently from the prospective of reliability.展开更多
Fasciola hepatica,an endemic parasite in Turkey,is still a very rare cause of cholestasis worldwide.Through ingestion of contaminated water plants like watercress,humans can become the definitive host of this parasite...Fasciola hepatica,an endemic parasite in Turkey,is still a very rare cause of cholestasis worldwide.Through ingestion of contaminated water plants like watercress,humans can become the definitive host of this parasite.Cholestatic symptoms may be sudden but in some cases they may be preceeded by a long period of fever,eosinophilia and vague gastrointestinal symptoms.We report a woman with cholangitis symptoms of sudden onset which was proved to be due to Fasciola hepatica infestation by an endoscopic retrograde cholangiography.展开更多
Objective: The present study was designed to investigate whether quercetin exerts increasing chemosensitivity on human lung adenocarcinoma cells when quercetin combined with cisplatin (DDP) and vincristine (VCR) ...Objective: The present study was designed to investigate whether quercetin exerts increasing chemosensitivity on human lung adenocarcinoma cells when quercetin combined with cisplatin (DDP) and vincristine (VCR) in vitro respectively and its possible antitumor mechanism. To provide experimental proof for clinical combination application. Methods: Using intermittent administration of high dose VCR, human lung adenocarcinoma sensitive cell line (A549/S) was induced to VCR- resistant human lung adenocarcinoma cell line (A549NCR). MTT assay was adapted for examing the 50% inhibition (IC50) value of DDP and VCR on A549/S and A549/VCR when quercetin combined with DDP and VCR respectively. Results: IC50 of DDP on A549/S and A549/VCR was 10.18 and 12.35 mg/L, and the IC50 of VCR on the two cell lines was 1.21 and 12.77 rag/L, respectively. The resistance fold of A549/VCR on VCR and DDP was 10.55 and 121, respectively. When quercetin at concentration of 50, 100 and 200 pmol/L in combination with DDP and VCR respectively, the IC50 of DDP and VCR on A549/S and A549/VCR were obvious decreased (P 〈 0.05 - P 〈 0.01). Conclusion: The experiment results suggested that quercetin could increase the chemosensitivity and partly revise the resistance of A549NCR.展开更多
AIM:To assess the rigorous relationship between human leukocyte antigens(HLA)-DR alleles and outcomes of hepatitis B virus(HBV) infections by means of metaanalysis.METHODS:Medline/PubMed,EMBASE,CNKI and VIP were searc...AIM:To assess the rigorous relationship between human leukocyte antigens(HLA)-DR alleles and outcomes of hepatitis B virus(HBV) infections by means of metaanalysis.METHODS:Medline/PubMed,EMBASE,CNKI and VIP were searched to identify relevant studies.Study quality was evaluated using the Newcastle-Ottawa Scale.Odds ratios(OR) and 95% confidence interval(95% CI) were pooled using Stata 11.0.Subgroup analyses were performed by ethnicity.Heterogeneity and publication bias analyses were performed to validate the credibility.RESULTS:A total of 2609 patients with chronic hepatitis B and 2606 controls spontaneously recovering from prior HBV infection were included.Meta-analysis showed that HLA-DR*04(OR = 0.72,95% CI:0.60-0.85) and DR*13(OR = 0.27,95% CI:0.19-0.37) alleles were significantly associated with HBV clearance while patients carrying HLA-DR*03(OR = 1.47,95% CI:1.16-1.87) or DR*07(OR = 1.59,95% CI:1.24-2.03) alleles had a significantly increased risk of chronic HBV persistence.For the HLA-DR*01 polymorphism,a significantly association with HBV clearance was found in Chinese Han group(OR = 0.48,95% CI:0.26-0.86),but not found in other ethnic groups(P = 0.191).For other polymorphisms,no association with the HBV infection outcome was found.CONCLUSION:HLA-DR*04 and DR*13 alleles may be the protective factors for HBV clearance and HLADR*03,and DR*07 alleles may be the risk factors for HBV persistence.展开更多
A new method for determining the freshness of harvested carp using nuclear imaging technology is presented.This research attempted to determine the freshness of carp by measuring the radioactive area increase (RAI) va...A new method for determining the freshness of harvested carp using nuclear imaging technology is presented.This research attempted to determine the freshness of carp by measuring the radioactive area increase (RAI) value of fish.The carp samples were maintained under refrigerated condition for 12d, and RAI and total volatile base nitrogen (T-VBN)values were determined during the storage. The results showed that the RAI value increased with the T-VBN content duringthe storage. Also, there is a significant correlation between T-VBN content and RAI value of the carp kept in refrigeratedcondition. The results of this study suggest that the RAI value could be utilized to evaluate rapidly the freshness of carp.展开更多
Conventional farming-pastoral ecotones methods of delineating were not quantitative and could not fully show their spatial distribution. The present paper attempts to develop quantitative methods for mapping farming-...Conventional farming-pastoral ecotones methods of delineating were not quantitative and could not fully show their spatial distribution. The present paper attempts to develop quantitative methods for mapping farming-pastoral ecotones in China. Nine indicators, related to temperature, precipitation and altitude aspects, were selected to quantify ecological susceptibility of vegetation (crops and forage). Methods of analytic hierarchy process (AHP) and expert score ranking combined with fuzzy set theory were applied to assign the weight for each indicator and to define the membership functions. The geographic information system (GIS) was used to manage the spatial database and conduct the spatial analysis. According to the spatial calculation of evaluation model integrated with GIS, the ecological susceptibility of vegetation (crops and forage) was mapped. Three different zones, pastoral area, farming-pastoral ecotones and farming area, were classified by spatial cluster analysis and the maximum likelihood classification for the numeric map of vegetation ecological susceptibility by GIS. This map was validated by the economic statistical result based on the ratio of the output value from animal husbandry in total output value of agriculture by the National Bureau of Statistics in China, indicating that the mapping of the farming-pastoral ecotones may be accepted.展开更多
Sense of touch is one of the important information from environment for human to live in daily life. Haptic interface is a hot topic in virtual reality but almost all of the devices focus on fingers and hands as targe...Sense of touch is one of the important information from environment for human to live in daily life. Haptic interface is a hot topic in virtual reality but almost all of the devices focus on fingers and hands as targets. In this paper, we focus on the foot haptic device with magnetic flied sensitive elastomer (MSE). We developed a haptic unit used as a magnetic field generator for MSE and contact point of foot haptic device. MSE samples mixed with 80 wt% carbonyl iron particles were prepared and evaluated with the developed magnet. Experimental results show that the mechanical property of the haptic unit can be modeled with the adjustable friction element. This property has a good advantage for the haptic unit.展开更多
A novel avian influenza A(H7N9) virus recently emerged in the Yangtze River delta and caused diseases, often severe, in over 130 people. This H7N9 virus appeared to infect humans with greater ease than previous avian ...A novel avian influenza A(H7N9) virus recently emerged in the Yangtze River delta and caused diseases, often severe, in over 130 people. This H7N9 virus appeared to infect humans with greater ease than previous avian influenza virus subtypes such as H5N1 and H9N2. While there are other potential explanations for this large number of human infections with an avian influenza virus, we investigated whether a lack of conserved T-cell epitopes between endemic H1N1 and H3N2 influenza viruses and the novel H7N9 virus contributes to this observation. Here we demonstrate that a number of T cell epitopes are conserved between endemic H1N1 and H3N2 viruses and H7N9 virus. Most of these conserved epitopes are from viral internal proteins. The extent of conservation between endemic human seasonal influenza and avian influenza H7N9 was comparable to that with the highly pathogenic avian influenza H5N1. Thus, the ease of inter-species transmission of H7N9 viruses(compared with avian H5N1 viruses) cannot be attributed to the lack of conservation of such T cell epitopes. On the contrary, our findings predict significant T-cell based cross-reactions in the human population to the novel H7N9 virus. Our findings also have implications for H7N9 virus vaccine design.展开更多
The object of the research is to compare the model performance and explain the error source of original logistic regression landslide susceptibility model(abbreviated as or-LRLSM) and landslide ratio-based logistic re...The object of the research is to compare the model performance and explain the error source of original logistic regression landslide susceptibility model(abbreviated as or-LRLSM) and landslide ratio-based logistic regression landslide susceptibility model(abbreviated as lr-LRLSM) in the Chishan watershed with a serious landslide disaster after 2009 Typhoon Morakot. The landslide inventory induced by 2009 Typhoon Morakot in South Taiwan is the main research material, while the Chishan watershed is the research area. Six variables, including elevation, slope, aspect, geological formation, accumulated rainfall, and bank erosion, were included in the two models. The performance of lr-LRLSM is better than that of or-LRLSM. The Cox & Snell R2, Nagelkerke R2 value, and the area under the relative operating characteristic curve(abbreviated as AUC) of lrLRLSM is larger than those of or-LRLSM, and the average correct ratio for the lr-LRLSM to predict landslide or non-landslide is larger than that of orLRLSM by 5.0%. The increase of the average correct ratio(abbreviated as ACR) difference from or-LRLSM to lr-LRLSM shows in slope, revised accumulated rainfall, aspect, geological formation and bank erosion variables, and only light decreases in elevation variable. The error sources of continuous variables in building the or-LRLSM is the dissimilarity between the distribution of landslide ratio and production of coefficient and characteristic values, while those of categorical variables is due to low correlation of landslide ratio and the coefficient value of each parameter. Using the classification of landslide ratio as the database to build logistic regression landslide susceptibility model(abbreviated as LRLSM) can revise the errors. The comparison of or-LRLSM and lr-LRLSM in the Chishan watershed also shows that building the landslide susceptibility model(abbreviated as LSM) by using lr-LRLSM is practical and of better performance than that by using the or-LRLSM.展开更多
We have performed a comparative theoretical study on the adsorption of nitric oxide (NO) on Zn12012 and Mgt2012 nanocages in terms of their energetic, geometric, and electronic properties. It has been found that NO ...We have performed a comparative theoretical study on the adsorption of nitric oxide (NO) on Zn12012 and Mgt2012 nanocages in terms of their energetic, geometric, and electronic properties. It has been found that NO adsorption on the MgO nanocage is energetically more favorable than that on the ZnO one. In contrast to the ZnO nanocage, HOMO-LUMO energy gap (Eg) of MgO one is dramatically decreased in the presence of NO molecule so that it is transformed from an intrinsic semiconductor (Eg≈5.00 eV) to a p-type one (Eg≈1.93 eV). We have predicted that electronic and conductance properties of the Mg12012 nanocage are sensitive toward NO molecule, thus it may be potential candidate in detection of NO molecules.展开更多
In DNA-guided hepatitis B treatment, viral load is insufficient, and requires other viral markers for treatment of hepatitis B patients as in patients with acute exacerbation of chronic hepatitis B, end-stage renal di...In DNA-guided hepatitis B treatment, viral load is insufficient, and requires other viral markers for treatment of hepatitis B patients as in patients with acute exacerbation of chronic hepatitis B, end-stage renal disease on dialysis, human immunodeficiency virus co-infected patients. There are exceptions to this rule: a residual level hepatitis B virus (HBV) DNA at 24 wk predicts beneficial outcome and reduced resistance at i year. The genotypic viral resistance to antiviral agents and occult HBV infection can be determined by HBV-DNA levels.展开更多
Ambient Assisted Living(AAL) is becoming an important research field. Many technologies have emerged related with pervasive computing vision, which can give support for AAL. One of the most reliable approaches is base...Ambient Assisted Living(AAL) is becoming an important research field. Many technologies have emerged related with pervasive computing vision, which can give support for AAL. One of the most reliable approaches is based on wireless sensor networks(WSNs). In this paper, we propose a coverage-aware unequal clustering protocol with load separation(CUCPLS) for data gathering of AAL applications based on WSNs. Firstly, the coverage overlap factor for nodes is introduced that accounts for the degree of target nodes covered. In addition, to balance the intra-cluster and inter-cluster energy consumptions, different competition radiuses of CHs are computed theoretically in different rings, and smaller clusters are formed near the sink. Moreover, two CHs are selected in each cluster for load separation to alleviate the substantial energy consumption difference between a single CH and its member nodes. Furthermore, a backoff waiting time is adopted during the selection of the two CHs to reduce the number of control messages employed. Simulation results demonstrate that the CUCPLS not only can achieve better coverage performance, but also balance the energy consumption of a network and prolong network lifetime.展开更多
Objective: To investigate the prevalence ofpenicillinase-producing Neisseria gonorrhoeae (PPNG) inWuxi. Methods: Nested PCR (nPCR) was used to test for thepresence of β-lactamase DNA in urethral or cervical swabsspec...Objective: To investigate the prevalence ofpenicillinase-producing Neisseria gonorrhoeae (PPNG) inWuxi. Methods: Nested PCR (nPCR) was used to test for thepresence of β-lactamase DNA in urethral or cervical swabsspecimens from 217 patients with gonorrhea. OnenPCR-positive product was randomly selected for DNAsequencing. Results: PPNG was found in 60 of all 217 specimens(27.65%), and in 33.87% (42/124) of male and 19.35%(18/93) of female cases. The nPCR product was identical toa known clinical PPNG strain. Conclusion: The prevalence of PPNG in STD patients inWuxi is alarmingly high, and this must be taken intoconsideration for successful treatment and prevention ofgonorrhea infections.展开更多
基金Financially supported by a grant from the Education Com-mittee of Hunan Province (No.ooAoo9)
文摘Objectives: To determine the susceptibilities of M.hominis and U. urealyticum to fluoroquinolones forthe instruction of reasonable clinical application ofantibiotics.Method: The susceptibilities of M. hominis and U.urealyticum to six fluoroquinolones were determinedby the broth dilution method.Results: Sparfloxacin and gatifloxacin were veryactive with MIC50S of 0.03125 and 0.25 μg/ml againstM. hominis, 0.25 and 0.5 μg/ml against U. urealyticum,respectively. Levofloxacin and ofloxacin had MIC50S of1 μg/ml and 2 μg/ml, respectively against both species.Norfloxacin was less effective against both species at16 and 32 μg/ml. Ciprofloxacin was unusual in thatthe MIC50S varied fourfold between species, with 2 μg/ml against M. hominis and 8 μg/ml against U.urealyticum.Conclusions: The results can provide useful infor-mation for selecting fluoroquinolones for treatmentof urogenital mycoplasma infections.
文摘This paper presents a comparative visualization strategy of slope failure susceptibility maps for analyzing different types of simultaneous occurrences of slope failures. Through the SEM (structural equation modeling), slope failure susceptibility maps are produced by using causal factors (i.e., geographical information, satellite remotely sensed data). As for a conventional pair-wise comparative procedure, the differences between susceptibility maps are delineated on difference maps, that can be, however, applied for evaluating differences only between pairs of susceptibility maps. One of the strong requirements from specialists working on slope stability evaluation is a comparative and visualization strategy of susceptibility maps with respect to "different types of simultaneous slope failures", for which the discussion is insufficient in the previous research activities for constructing the quantitative models for slope failure hazard mapping. As a measure, a color composite map based on susceptibility maps has been produced. The combination of assigning susceptibility maps to RGB-color planes is determined based on an index of "NCCT (normalized correlated color temperature)" which represents the relationship between chromaticity and human visual perception. Through the cases examined, the result indicates that the proposed color composite map, as a heuristic visualization strategy, is useful for simultaneously evaluating the hazardous areas affected by "different types of slope failures".
文摘Influence factors of frozen soil blastability are analyzed which mainly conclude the strain energy coefficient, tensile strength, compressive strength, longitudinal wave velocity and transverse wave velocity. According to the principle of perceptron neural network, at first the index factors are standardized by the aid of the efficient function theory, then the blastability of frozen sand at -7, -12 and -17 ℃ are classified three categories. Through adjusting the weight value and threshold value, we can obtain that the clay blastability at -7 ℃ is close to the sand blastability at -12 ℃, they belong to the second category, the clay blastability at -12 ℃ is close to the sand blastability at -17 ℃, thus they are divided into the third category.
基金supported in part by the National Key S&T Special Projects on Major Infectious Diseases (Grant No. 2008ZX10001-002, 2008ZX10001-012)the National Natural Science Foundation of China (No. 30700706)
文摘Neutralizing antibodies are considered to be an important protective parameter used in HIV-1 vaccine evaluation. However, the exact role that neutralizing antibodies plays in controlling the disease progression of HIV-1 infected peoples is still undetermined. In this paper, we compared the protective function of the neutralizing antibody response in the plasma from LTNP and TP against clade B and clade C pseudoviruses. No difference in the neutralizing activities between the plasma from LTNP and TP was found, which was consistent with the most recent reports. In addition, no correlations between the titer or breadth and CD4+ or viral load in HIV-1 infected individuals were found. The protective roles played by neutralizing antibodies in controlling disease progression of HIV-1 infected people need to be considered in a new viewpoint.
基金the National Natural Science Foundation of China
文摘In underwater target detection, the bottom reverberation has some of the same properties as the target echo, which has a great impact on the performance. It is essential to study the difference between target echo and reverberation. In this paper, based on the unique advantage of human listening ability on objects distinction, the Gammatone filter is taken as the auditory model. In addition, time-frequency perception features and auditory spectral features are extracted for active sonar target echo and bottom reverberation separation. The features of the experimental data have good concentration characteristics in the same class and have a large amount of differences between different classes, which shows that this method can effectively distinguish between the target echo and reverberation.
基金This work was supported by National Defence Advanced Research Fund .Serial No.5141604010HT0117
文摘To Integrate the capacity of sensing, communication, computing, and actuating, one of the compelling technological advances of these years has been the appearance of distributed wireless sensor network (DSN) for information gathering tasks. In order to save the energy, multi-hop routing between the sensor nodes and the sink node is necessary because of limited resource. In addition, the unpredictable conditional factors make the sensor nodes unreliable. In this paper, the reliability of routing designed for sensor network and some dependability issues of DSN, such as MTTF (mean time to failure) and the probability of connectivity between the sensor nodes and the sink node are analyzed. Unfortunately, we could not obtain the accurate result for the arbitrary network topology, which is #P-hard problem. And the reliability analysis of restricted topologies clustering-based is given. The method proposed in this paper will show us a constructive idea about how to place energy-constrained sensor nodes in the network efficiently from the prospective of reliability.
文摘Fasciola hepatica,an endemic parasite in Turkey,is still a very rare cause of cholestasis worldwide.Through ingestion of contaminated water plants like watercress,humans can become the definitive host of this parasite.Cholestatic symptoms may be sudden but in some cases they may be preceeded by a long period of fever,eosinophilia and vague gastrointestinal symptoms.We report a woman with cholangitis symptoms of sudden onset which was proved to be due to Fasciola hepatica infestation by an endoscopic retrograde cholangiography.
基金Supported by a grant from the Health Department of Jiangxi Province for Science Technical Program Foundation(No.2005-22A)
文摘Objective: The present study was designed to investigate whether quercetin exerts increasing chemosensitivity on human lung adenocarcinoma cells when quercetin combined with cisplatin (DDP) and vincristine (VCR) in vitro respectively and its possible antitumor mechanism. To provide experimental proof for clinical combination application. Methods: Using intermittent administration of high dose VCR, human lung adenocarcinoma sensitive cell line (A549/S) was induced to VCR- resistant human lung adenocarcinoma cell line (A549NCR). MTT assay was adapted for examing the 50% inhibition (IC50) value of DDP and VCR on A549/S and A549/VCR when quercetin combined with DDP and VCR respectively. Results: IC50 of DDP on A549/S and A549/VCR was 10.18 and 12.35 mg/L, and the IC50 of VCR on the two cell lines was 1.21 and 12.77 rag/L, respectively. The resistance fold of A549/VCR on VCR and DDP was 10.55 and 121, respectively. When quercetin at concentration of 50, 100 and 200 pmol/L in combination with DDP and VCR respectively, the IC50 of DDP and VCR on A549/S and A549/VCR were obvious decreased (P 〈 0.05 - P 〈 0.01). Conclusion: The experiment results suggested that quercetin could increase the chemosensitivity and partly revise the resistance of A549NCR.
基金Supported by The National Natural Science Foundation of China,No.30972598National Basic Research Program of China (973 Program),No.2007CB512903the State Key Project Specialized for Infectious Diseases,No.2008ZX10002-007
文摘AIM:To assess the rigorous relationship between human leukocyte antigens(HLA)-DR alleles and outcomes of hepatitis B virus(HBV) infections by means of metaanalysis.METHODS:Medline/PubMed,EMBASE,CNKI and VIP were searched to identify relevant studies.Study quality was evaluated using the Newcastle-Ottawa Scale.Odds ratios(OR) and 95% confidence interval(95% CI) were pooled using Stata 11.0.Subgroup analyses were performed by ethnicity.Heterogeneity and publication bias analyses were performed to validate the credibility.RESULTS:A total of 2609 patients with chronic hepatitis B and 2606 controls spontaneously recovering from prior HBV infection were included.Meta-analysis showed that HLA-DR*04(OR = 0.72,95% CI:0.60-0.85) and DR*13(OR = 0.27,95% CI:0.19-0.37) alleles were significantly associated with HBV clearance while patients carrying HLA-DR*03(OR = 1.47,95% CI:1.16-1.87) or DR*07(OR = 1.59,95% CI:1.24-2.03) alleles had a significantly increased risk of chronic HBV persistence.For the HLA-DR*01 polymorphism,a significantly association with HBV clearance was found in Chinese Han group(OR = 0.48,95% CI:0.26-0.86),but not found in other ethnic groups(P = 0.191).For other polymorphisms,no association with the HBV infection outcome was found.CONCLUSION:HLA-DR*04 and DR*13 alleles may be the protective factors for HBV clearance and HLADR*03,and DR*07 alleles may be the risk factors for HBV persistence.
文摘A new method for determining the freshness of harvested carp using nuclear imaging technology is presented.This research attempted to determine the freshness of carp by measuring the radioactive area increase (RAI) value of fish.The carp samples were maintained under refrigerated condition for 12d, and RAI and total volatile base nitrogen (T-VBN)values were determined during the storage. The results showed that the RAI value increased with the T-VBN content duringthe storage. Also, there is a significant correlation between T-VBN content and RAI value of the carp kept in refrigeratedcondition. The results of this study suggest that the RAI value could be utilized to evaluate rapidly the freshness of carp.
基金supported by the National Western Special Project (Project No. 2003BA901A20)
文摘Conventional farming-pastoral ecotones methods of delineating were not quantitative and could not fully show their spatial distribution. The present paper attempts to develop quantitative methods for mapping farming-pastoral ecotones in China. Nine indicators, related to temperature, precipitation and altitude aspects, were selected to quantify ecological susceptibility of vegetation (crops and forage). Methods of analytic hierarchy process (AHP) and expert score ranking combined with fuzzy set theory were applied to assign the weight for each indicator and to define the membership functions. The geographic information system (GIS) was used to manage the spatial database and conduct the spatial analysis. According to the spatial calculation of evaluation model integrated with GIS, the ecological susceptibility of vegetation (crops and forage) was mapped. Three different zones, pastoral area, farming-pastoral ecotones and farming area, were classified by spatial cluster analysis and the maximum likelihood classification for the numeric map of vegetation ecological susceptibility by GIS. This map was validated by the economic statistical result based on the ratio of the output value from animal husbandry in total output value of agriculture by the National Bureau of Statistics in China, indicating that the mapping of the farming-pastoral ecotones may be accepted.
文摘Sense of touch is one of the important information from environment for human to live in daily life. Haptic interface is a hot topic in virtual reality but almost all of the devices focus on fingers and hands as targets. In this paper, we focus on the foot haptic device with magnetic flied sensitive elastomer (MSE). We developed a haptic unit used as a magnetic field generator for MSE and contact point of foot haptic device. MSE samples mixed with 80 wt% carbonyl iron particles were prepared and evaluated with the developed magnet. Experimental results show that the mechanical property of the haptic unit can be modeled with the adjustable friction element. This property has a good advantage for the haptic unit.
基金supported in part by General Research Fund, Research Grants Council of Hong Kong (HKU 780113M)Area of Excellence program (AoE/M-12/06)+1 种基金University Grants Committee of Hong Kong SARResearch Fund for the Control of Infectious Diseases, Hong Kong SAR government (11100742)
文摘A novel avian influenza A(H7N9) virus recently emerged in the Yangtze River delta and caused diseases, often severe, in over 130 people. This H7N9 virus appeared to infect humans with greater ease than previous avian influenza virus subtypes such as H5N1 and H9N2. While there are other potential explanations for this large number of human infections with an avian influenza virus, we investigated whether a lack of conserved T-cell epitopes between endemic H1N1 and H3N2 influenza viruses and the novel H7N9 virus contributes to this observation. Here we demonstrate that a number of T cell epitopes are conserved between endemic H1N1 and H3N2 viruses and H7N9 virus. Most of these conserved epitopes are from viral internal proteins. The extent of conservation between endemic human seasonal influenza and avian influenza H7N9 was comparable to that with the highly pathogenic avian influenza H5N1. Thus, the ease of inter-species transmission of H7N9 viruses(compared with avian H5N1 viruses) cannot be attributed to the lack of conservation of such T cell epitopes. On the contrary, our findings predict significant T-cell based cross-reactions in the human population to the novel H7N9 virus. Our findings also have implications for H7N9 virus vaccine design.
基金Ministry of Science and Technology in Taiwan for providing budget for my project (project number:NSC 103-2313-B-035-001)
文摘The object of the research is to compare the model performance and explain the error source of original logistic regression landslide susceptibility model(abbreviated as or-LRLSM) and landslide ratio-based logistic regression landslide susceptibility model(abbreviated as lr-LRLSM) in the Chishan watershed with a serious landslide disaster after 2009 Typhoon Morakot. The landslide inventory induced by 2009 Typhoon Morakot in South Taiwan is the main research material, while the Chishan watershed is the research area. Six variables, including elevation, slope, aspect, geological formation, accumulated rainfall, and bank erosion, were included in the two models. The performance of lr-LRLSM is better than that of or-LRLSM. The Cox & Snell R2, Nagelkerke R2 value, and the area under the relative operating characteristic curve(abbreviated as AUC) of lrLRLSM is larger than those of or-LRLSM, and the average correct ratio for the lr-LRLSM to predict landslide or non-landslide is larger than that of orLRLSM by 5.0%. The increase of the average correct ratio(abbreviated as ACR) difference from or-LRLSM to lr-LRLSM shows in slope, revised accumulated rainfall, aspect, geological formation and bank erosion variables, and only light decreases in elevation variable. The error sources of continuous variables in building the or-LRLSM is the dissimilarity between the distribution of landslide ratio and production of coefficient and characteristic values, while those of categorical variables is due to low correlation of landslide ratio and the coefficient value of each parameter. Using the classification of landslide ratio as the database to build logistic regression landslide susceptibility model(abbreviated as LRLSM) can revise the errors. The comparison of or-LRLSM and lr-LRLSM in the Chishan watershed also shows that building the landslide susceptibility model(abbreviated as LSM) by using lr-LRLSM is practical and of better performance than that by using the or-LRLSM.
文摘We have performed a comparative theoretical study on the adsorption of nitric oxide (NO) on Zn12012 and Mgt2012 nanocages in terms of their energetic, geometric, and electronic properties. It has been found that NO adsorption on the MgO nanocage is energetically more favorable than that on the ZnO one. In contrast to the ZnO nanocage, HOMO-LUMO energy gap (Eg) of MgO one is dramatically decreased in the presence of NO molecule so that it is transformed from an intrinsic semiconductor (Eg≈5.00 eV) to a p-type one (Eg≈1.93 eV). We have predicted that electronic and conductance properties of the Mg12012 nanocage are sensitive toward NO molecule, thus it may be potential candidate in detection of NO molecules.
文摘In DNA-guided hepatitis B treatment, viral load is insufficient, and requires other viral markers for treatment of hepatitis B patients as in patients with acute exacerbation of chronic hepatitis B, end-stage renal disease on dialysis, human immunodeficiency virus co-infected patients. There are exceptions to this rule: a residual level hepatitis B virus (HBV) DNA at 24 wk predicts beneficial outcome and reduced resistance at i year. The genotypic viral resistance to antiviral agents and occult HBV infection can be determined by HBV-DNA levels.
基金supported by the National Nature Science Foundation of China (61170169, 61170168)
文摘Ambient Assisted Living(AAL) is becoming an important research field. Many technologies have emerged related with pervasive computing vision, which can give support for AAL. One of the most reliable approaches is based on wireless sensor networks(WSNs). In this paper, we propose a coverage-aware unequal clustering protocol with load separation(CUCPLS) for data gathering of AAL applications based on WSNs. Firstly, the coverage overlap factor for nodes is introduced that accounts for the degree of target nodes covered. In addition, to balance the intra-cluster and inter-cluster energy consumptions, different competition radiuses of CHs are computed theoretically in different rings, and smaller clusters are formed near the sink. Moreover, two CHs are selected in each cluster for load separation to alleviate the substantial energy consumption difference between a single CH and its member nodes. Furthermore, a backoff waiting time is adopted during the selection of the two CHs to reduce the number of control messages employed. Simulation results demonstrate that the CUCPLS not only can achieve better coverage performance, but also balance the energy consumption of a network and prolong network lifetime.
文摘Objective: To investigate the prevalence ofpenicillinase-producing Neisseria gonorrhoeae (PPNG) inWuxi. Methods: Nested PCR (nPCR) was used to test for thepresence of β-lactamase DNA in urethral or cervical swabsspecimens from 217 patients with gonorrhea. OnenPCR-positive product was randomly selected for DNAsequencing. Results: PPNG was found in 60 of all 217 specimens(27.65%), and in 33.87% (42/124) of male and 19.35%(18/93) of female cases. The nPCR product was identical toa known clinical PPNG strain. Conclusion: The prevalence of PPNG in STD patients inWuxi is alarmingly high, and this must be taken intoconsideration for successful treatment and prevention ofgonorrhea infections.