The construction of the first infectious clone JFH-1 speeds up the research on hepatitis C virus (HCV). However, Huh7 cell line was the only highly permissive cell line for HCV infection and only a few clones were ful...The construction of the first infectious clone JFH-1 speeds up the research on hepatitis C virus (HCV). However, Huh7 cell line was the only highly permissive cell line for HCV infection and only a few clones were fully permissive. In this study, two different fully permissive clones of Huh7 cells, Huh7.5.1 and Huh7-Lunet-CD81 (Lunet-CD81) cells were compared for their responses upon HCV infection. The virus replication level was found slightly higher in Huh7.5.1 cells than that in Lunet-CD81 cells. Viability of Huh7.5.1 cells but not of Lunet-CD81 cells was reduced significantly after HCV infection. Further analysis showed that the cell cycle of infected Huh7.5.1 cells was arrested at G1 phase. The G1/S transition was blocked by HCV infection in Huh7.5.1 cells as shown by the cell cycle synchronization analysis. Genes related to cell cycle regulation was modified by HCV infection and gene interaction analysis in GeneSpring GX in Direct Interactions mode highlighted 31 genes. In conclusion, the responses of those two cell lines were different upon HCV infection. HCV infection blocked G1/S transition and cell cycle progress, thus reduced the cell viability in Huh7.5.1 cells but not in Lunet-CD81 cells. Lunet-CD81 cells might be suitable for long term infection studies of HCV.展开更多
The so-called emotional education is the education of cultivating students＇ social emotion, promoting their self-control ability and promoting students to correctly cope with the relationship between themselves and s...The so-called emotional education is the education of cultivating students＇ social emotion, promoting their self-control ability and promoting students to correctly cope with the relationship between themselves and surrounding environment. In college English teaching, implementing emotional education not only can teach knowledge to students, but also can help others to form perfect personality, to promote all-round development of students. However, fi＇om the present situation, in college English teaching, it generally exists ＂focusing more on knowledge and less on emotion＂ phenomenon, which doesn＇t conform to the requirement of quality education. In college English teaching, conducting emotional education is necessary and imminent.展开更多
基金supported partly by grants of National Nature Science Foundation of China (grant 31200135)
文摘The construction of the first infectious clone JFH-1 speeds up the research on hepatitis C virus (HCV). However, Huh7 cell line was the only highly permissive cell line for HCV infection and only a few clones were fully permissive. In this study, two different fully permissive clones of Huh7 cells, Huh7.5.1 and Huh7-Lunet-CD81 (Lunet-CD81) cells were compared for their responses upon HCV infection. The virus replication level was found slightly higher in Huh7.5.1 cells than that in Lunet-CD81 cells. Viability of Huh7.5.1 cells but not of Lunet-CD81 cells was reduced significantly after HCV infection. Further analysis showed that the cell cycle of infected Huh7.5.1 cells was arrested at G1 phase. The G1/S transition was blocked by HCV infection in Huh7.5.1 cells as shown by the cell cycle synchronization analysis. Genes related to cell cycle regulation was modified by HCV infection and gene interaction analysis in GeneSpring GX in Direct Interactions mode highlighted 31 genes. In conclusion, the responses of those two cell lines were different upon HCV infection. HCV infection blocked G1/S transition and cell cycle progress, thus reduced the cell viability in Huh7.5.1 cells but not in Lunet-CD81 cells. Lunet-CD81 cells might be suitable for long term infection studies of HCV.
文摘The so-called emotional education is the education of cultivating students＇ social emotion, promoting their self-control ability and promoting students to correctly cope with the relationship between themselves and surrounding environment. In college English teaching, implementing emotional education not only can teach knowledge to students, but also can help others to form perfect personality, to promote all-round development of students. However, fi＇om the present situation, in college English teaching, it generally exists ＂focusing more on knowledge and less on emotion＂ phenomenon, which doesn＇t conform to the requirement of quality education. In college English teaching, conducting emotional education is necessary and imminent.
