人工感染仔猪猪生殖与呼吸综合征病毒的核酸分布规律

根据猪生殖与呼吸综合征病毒（PRRsV）美洲株ATCCVR-2332保守区ORF6和ORF7的基因序列，设计了1条37bp的寡核苷酸探针，末端用生物素标记。应用原位杂交方法对人工感染PRRSVSC-1株的28日龄仔猪进行了病毒核酸分布规律的研究。结果显示，感染后7～28d于肝、脾、肺、肾、扁桃体、胸腺、肺门淋巴结、肠和大脑检测到阳性杂交信号，其中肺门淋巴结的阳性信号一直较强，脾的阳性信号逐渐增强，肺的阳性信号较弱，心脏未见阳性杂交信号。

感染仔猪粪肠球菌不同分离株的鉴定及毒力基因检测

从近年来河南省各地感染发病猪群的肠球菌分离株中,选取来源不同且具有代表性的5株分离菌进行了包括致病性和毒力基因测定在内的系统鉴定。结果表明,引起本次河南省仔猪感染发病的病原体为粪肠球菌。各地分离菌的形态、培养特性与以及对极端环境的耐受性上表现较为一致,而对各种糖的发酵上存在着的差异;对药物万古霉素、替考拉宁、利福平和氨苄西林敏感,而对临床常用药物红霉素、卡那霉素和四环素完全耐药;经16S rRNA测定,它们与粪肠球菌ATCC29212同源性在99.6%～99.8%之间,与GenBank公布的NC_004668、AJ301831的核苷酸同源性为99.7%～99.9%和99.5%～99.7%;通过对它们2种毒力表型和携带的6种主要毒力基因以及与对小鼠的LD50测定,发现5株粪肠球菌携带毒力基因不尽相同,携带全部6种毒力基因的HE1和HE5的致病力最强,而仅携带4种毒力基因的HE41致病力最小。用HE1和HE5分离菌对20日龄的断奶仔猪分别进行攻毒,2菌株均能引起仔猪的感染发病。

猪繁殖与呼吸综合征和仔猪副伤寒混合感染的诊断和防制

2014年3月5日,新疆北屯市某养猪户从石河子购进断奶仔猪58头,饲喂新疆乌鲁木齐正大公司生产的乳猪料,未做任何免疫工作,前期生长良好.4月19日该猪群发生呼吸急促,耳朵、臀部等处皮肤出现蓝紫色,拉黄绿色腥臭的稀粪,体温高为特征的疫病,畜主发现病情后曾用青霉素、链霉素、磺胺嘧啶注射液进行治疗,均无疗效,病死猪数量迅速增加,发病数为42头,发病率为72.4％ （42/58）,死亡数为29头,死亡率为50％（29/58）,病程6d.

仔猪呼吸道病混合感染的诊治报告

广西某瘦肉型猪场发生一起以断奶仔猪发热、流鼻涕、咳嗽、死亡率高为主要特征的严重呼吸道疾病,根据流行病学、临床症状,病理变化和实验室检查,诊断为多种病原引起的呼吸道综合征。采用氟苯尼考、克林霉素、头孢拉定和中草药相结合的疗法,有效的控制了疾病的蔓延。结果:有效率达90%,治愈率达82.3%。

猪混合感染猪瘟和仔猪副伤寒的诊断及疗效观察

奇台县西地乡两家养殖户,喂养的152头猪于2004年1月26日前后分别开始发病,并陆续死亡,根据流行病学调查,临床症状观察,病理解剖变化和实验室检查,确诊为猪瘟和仔猪副伤寒混合感染,立即采取措施,于2月12日基本控制了该病。

Piglet Diarrhea and Technology Transfers to Prevent E. coli Infection at Small-Holder Pig Farms in Binh Dinh, Vietnam

The study aims at finding alternative interventions for the prevention ofE. coli infection in piglets so as to boost farmers＇ benefits and improve environment in the area. Data were collected from one experimental group consisting of 210 small pig holder farms and one control group of 70 farms. All of 280 litters and sows were analyzed and the techniques of early vaccination at 60-80 d of pregnant （time 1） were applied and repeated after 10 d with E. coli vaccine to produce antibodies through milk. The percentage of piglets with diarrhea caused by E. coli significantly decreased from 75.7% to 18.9%. Injection of E. coli vaccine for the pregnant sows created antibodies for young piglets through colostrums. The treatment of E. coli cost 45,420 Vietnam Dong （VND） per litter in the experimental group and that was 156,000 VND/litter in the control group. Use of betel-bokashi （bio-product as plant antibiotics and Lactobacillus） to treat piglet diarrhea was proved safe and effective. Litter size and average weight at birth and weaning were improved significantly （P 〈 0.05）. The average weaned weight of piglets and sows were 11.2 kg and 118.6 kg in the experimental group, compared to 9.4 kg and 89.9 kg in the control group, respectively. Piglets in the experimental group had good health, no diarrhea and a shorter weaning time （2 d） and mating interval 2.22 days earlier than those in the control group. The average profit of 210 households was significantly higher in the experiment group： feed-cost was much lower than that in the control group. Small-holder farms implementing the experiments of applying the techniques received more financial profit, which was 1,502,000 VND per year per sow, while those who did not apply the techniques gained only 223,000 VND per year per sow.

浅析如何鉴别、治疗仔猪混合感染（猪瘟病毒、大肠杆菌）

猪仔的混合感染的鉴定需要在气候变化的环境下进行分析，主要针对猪仔在气候条件急剧变化下发生的过激反应等进行鉴别，对猪体内的大肠杆菌的繁殖导致的猪仔的免疫力降低等情况，对免疫力降低导致的猪瘟等混合型感染的情况进行检验和检测。检测的手段一般采用实验室化验的方法，经过综合的检验检测鉴别大肠杆菌和猪瘟病毒，从而有针对性地采取有利措施对病情加以控制。