Schistosomiasis is a serious public health problem in the middle-lower Yangtze River Basin in China. Study of spatial variation of snail distribution that is related to microgeographic factors can help to choose perti...Schistosomiasis is a serious public health problem in the middle-lower Yangtze River Basin in China. Study of spatial variation of snail distribution that is related to microgeographic factors can help to choose pertinent measures for snail extinguishment and environment rebuilding. This paper studied the theoretical architecture of weights-of-evidence approach. The case study was made for spatial relation between the occurrence of infected snails and geographic factor combinations in Waijiazhou marshland of Poyang Lake region in China. The multievidence data came from the geographical factor combinations by crossing operation of vegetation coverage grade layer, cattle route distance grade layer, and special environment layer (181 combinations in total) in GIS. The calculation of weight contrast index shows that high vegetation coverage, cattle route distance of <45 meters, and special geographic factor "ground depression" had direct spatial relation with the occurrence of infected snails. The verification by crossing operation in GIS indicated 72.45% of the infected snails concentrated on the areas of positive weight contrast index (sequenced in an order of weight contrast index from high to low), demonstrating the high efficiency of the model established in finding infected snails according to the geographic factor combinations that can be explicitly discerned in the study area.展开更多
Monochamus alternatus, the main vector beetles of invasive pinewood nematode, has established a symbiotic relationship with a native ectotrophic fungal symbiont, Sporothrix sp. 1, in China. The immune response ofM. al...Monochamus alternatus, the main vector beetles of invasive pinewood nematode, has established a symbiotic relationship with a native ectotrophic fungal symbiont, Sporothrix sp. 1, in China. The immune response ofM. alternatus to S. sp. 1 in the coexistence of beetles and fungi is, however, unknown. Here, we report that immune responses ofM. alternatus pupae to infection caused by ectotrophic symbiotic fungus S. sp. 1 and entomopathogenic fungus Beauveria bassiana differ significantly. The S. sp. 1 did not kill the beetles while B. bassiana killed all upon injection. The transcriptome results showed that the numbers of differentially expressed genes in M. aIternatus infected with S. sp. 1 were 2-fold less than those infected with B. bassiana at 48 hours post infection. It was noticed that Toll and IMD pathways played a leading role in the beetle＇s immune system when infected by symbiotic fungus, but upon infection by entomopathogenic fimgus, only the Toll pathway gets triggered actively. Furthermore, the beetles could tolerate the infection of symbiotic fungi by retracing their Toll and IMD pathways at 48 h. This study provided a comprehensive sequence resource ofM. alternatus transcriptome for further study of the immune interactions between host and associated fungi.展开更多
基金Supported by a the National Natural Science Fundation of China (No. 30590370)the Research Project "Spatial Simulation of Schistosomiasis Susceptible Areas in the Poyang Lake Region" Sponsored by Science Research Plan 2007 of Jiangxi Normal University (Natural Science Category)
文摘Schistosomiasis is a serious public health problem in the middle-lower Yangtze River Basin in China. Study of spatial variation of snail distribution that is related to microgeographic factors can help to choose pertinent measures for snail extinguishment and environment rebuilding. This paper studied the theoretical architecture of weights-of-evidence approach. The case study was made for spatial relation between the occurrence of infected snails and geographic factor combinations in Waijiazhou marshland of Poyang Lake region in China. The multievidence data came from the geographical factor combinations by crossing operation of vegetation coverage grade layer, cattle route distance grade layer, and special environment layer (181 combinations in total) in GIS. The calculation of weight contrast index shows that high vegetation coverage, cattle route distance of <45 meters, and special geographic factor "ground depression" had direct spatial relation with the occurrence of infected snails. The verification by crossing operation in GIS indicated 72.45% of the infected snails concentrated on the areas of positive weight contrast index (sequenced in an order of weight contrast index from high to low), demonstrating the high efficiency of the model established in finding infected snails according to the geographic factor combinations that can be explicitly discerned in the study area.
基金supported by the Forestry Industry Research Special Funds for Public Welfare Project(201204501)National Key Plan for Scientific Research and Development of China(2016YFC1200604,2016YFD0500300)+2 种基金the High Technology Research and Development Program(HTRDP)of China(2014AA020529)National Natural Science Foundation of China(31572272,31370650,31402013,31221091,31672291,L1524009)the CAS Key Research Projects of the Frontier Science(QYZDBSSW-SMC014),and CAS(2015-SM-C-02)
文摘Monochamus alternatus, the main vector beetles of invasive pinewood nematode, has established a symbiotic relationship with a native ectotrophic fungal symbiont, Sporothrix sp. 1, in China. The immune response ofM. alternatus to S. sp. 1 in the coexistence of beetles and fungi is, however, unknown. Here, we report that immune responses ofM. alternatus pupae to infection caused by ectotrophic symbiotic fungus S. sp. 1 and entomopathogenic fungus Beauveria bassiana differ significantly. The S. sp. 1 did not kill the beetles while B. bassiana killed all upon injection. The transcriptome results showed that the numbers of differentially expressed genes in M. aIternatus infected with S. sp. 1 were 2-fold less than those infected with B. bassiana at 48 hours post infection. It was noticed that Toll and IMD pathways played a leading role in the beetle＇s immune system when infected by symbiotic fungus, but upon infection by entomopathogenic fimgus, only the Toll pathway gets triggered actively. Furthermore, the beetles could tolerate the infection of symbiotic fungi by retracing their Toll and IMD pathways at 48 h. This study provided a comprehensive sequence resource ofM. alternatus transcriptome for further study of the immune interactions between host and associated fungi.
