筛选和鉴定水稻卷叶突变体是研究叶片形态建成机理的前提。本研究利用甲基磺酸乙酯对常规粳稻秀水09进行诱变,获得了一个稳定遗传的类感干尖线虫卷叶突变体（nematode infestation mimic rolling leaf mutant,nir）。与野生型相比,nir...筛选和鉴定水稻卷叶突变体是研究叶片形态建成机理的前提。本研究利用甲基磺酸乙酯对常规粳稻秀水09进行诱变,获得了一个稳定遗传的类感干尖线虫卷叶突变体（nematode infestation mimic rolling leaf mutant,nir）。与野生型相比,nir全生育期叶尖卷曲、枯萎,表型与干尖线虫侵染症状相似。此外,突变体植株矮小,叶片表面较光滑,有效分蘖数少。采用nir与秀水09杂交的F2群体进行遗传分析,结果表明nir的类感干尖线虫卷叶性状受一对隐性核基因控制。利用nir与籼稻93-11杂交获得的F2群体,采用分子标记技术将目的基因最终定位在水稻第二染色体2~88w和2~82w之间,遗传距离为1.1 c M,物理距离245 kb。研究结果表明,nir很可能是一个尚未报道的新基因,进一步克隆nir基因有可能揭示与现有报道的卷叶基因不同的卷叶调控机理,为筛选理想株型、培育高产水稻品种提供理论基础和供试材料。展开更多
[Objective] The aim was to explore the feasibility of using single spectrum image to classify crops based on multi-spectral image and Decision Tree Method. [Method] Taking the typical agriculture plantation area in Hu...[Objective] The aim was to explore the feasibility of using single spectrum image to classify crops based on multi-spectral image and Decision Tree Method. [Method] Taking the typical agriculture plantation area in Hulunbeier area, according to field measured spectrum data, the optimum time of main crops, barley, wheat, rapeseed, based on crops spectrum characteristics, by dint of decision-making tree method, and considering spectral matching method, classification of crops was studied such as SAM. [Result] By dint of Landsat TM image gained in the first half of August, based on geographic and atmospheric proof-reading, decision-making tree was constructed. Plantation information about wheat, barley, and rapeseed and plantation grassland was extracted successfully. The general classification accuracy reached 86.90%. Kappa coefficient was 0.831 1. [Conclusion] Taking typical spectrum image as data source, and applying Decision Tree Method to get crops type＇s information had fine application future.展开更多
In order to recognize people＇s annoyance emotions in the working environment and evaluate emotional well- being, emotional speech in a work environment is induced to obtain adequate samples of emotional speech, and a...In order to recognize people＇s annoyance emotions in the working environment and evaluate emotional well- being, emotional speech in a work environment is induced to obtain adequate samples of emotional speech, and a Mandarin database with two thousands samples is built. In searching for annoyance-type emotion features, the prosodic feature and the voice quality feature parameters of the emotional statements are extracted first. Then an improved back propagation （BP） neural network based on the shuffled frog leaping algorithm （SFLA） is proposed to recognize the emotion. The recognition capability of the BP, radical basis function （RBF） and the SFLA neural networks are compared experimentally. The results show that the recognition ratio of the SFLA neural network is 4. 7% better than that of the BP neural network and 4. 3% better than that of the RBF neural network. The experimental results demonstrate that the random initial data trained by the SFLA can optimize the connection weights and thresholds of the neural network, speed up the convergence and improve the recognition rate.展开更多
Objectives: To determine the susceptibilities of M.hominis and U. urealyticum to fluoroquinolones forthe instruction of reasonable clinical application ofantibiotics.Method: The susceptibilities of M. hominis and U.ur...Objectives: To determine the susceptibilities of M.hominis and U. urealyticum to fluoroquinolones forthe instruction of reasonable clinical application ofantibiotics.Method: The susceptibilities of M. hominis and U.urealyticum to six fluoroquinolones were determinedby the broth dilution method.Results: Sparfloxacin and gatifloxacin were veryactive with MIC50S of 0.03125 and 0.25 μg/ml againstM. hominis, 0.25 and 0.5 μg/ml against U. urealyticum,respectively. Levofloxacin and ofloxacin had MIC50S of1 μg/ml and 2 μg/ml, respectively against both species.Norfloxacin was less effective against both species at16 and 32 μg/ml. Ciprofloxacin was unusual in thatthe MIC50S varied fourfold between species, with 2 μg/ml against M. hominis and 8 μg/ml against U.urealyticum.Conclusions: The results can provide useful infor-mation for selecting fluoroquinolones for treatmentof urogenital mycoplasma infections.展开更多
文摘筛选和鉴定水稻卷叶突变体是研究叶片形态建成机理的前提。本研究利用甲基磺酸乙酯对常规粳稻秀水09进行诱变,获得了一个稳定遗传的类感干尖线虫卷叶突变体（nematode infestation mimic rolling leaf mutant,nir）。与野生型相比,nir全生育期叶尖卷曲、枯萎,表型与干尖线虫侵染症状相似。此外,突变体植株矮小,叶片表面较光滑,有效分蘖数少。采用nir与秀水09杂交的F2群体进行遗传分析,结果表明nir的类感干尖线虫卷叶性状受一对隐性核基因控制。利用nir与籼稻93-11杂交获得的F2群体,采用分子标记技术将目的基因最终定位在水稻第二染色体2~88w和2~82w之间,遗传距离为1.1 c M,物理距离245 kb。研究结果表明,nir很可能是一个尚未报道的新基因,进一步克隆nir基因有可能揭示与现有报道的卷叶基因不同的卷叶调控机理,为筛选理想株型、培育高产水稻品种提供理论基础和供试材料。
基金Supported by the Open Subject of Key Lab of Resources Remote-sensing and Digital Agriculture in Agricultural Department(RDA1008)~~
文摘[Objective] The aim was to explore the feasibility of using single spectrum image to classify crops based on multi-spectral image and Decision Tree Method. [Method] Taking the typical agriculture plantation area in Hulunbeier area, according to field measured spectrum data, the optimum time of main crops, barley, wheat, rapeseed, based on crops spectrum characteristics, by dint of decision-making tree method, and considering spectral matching method, classification of crops was studied such as SAM. [Result] By dint of Landsat TM image gained in the first half of August, based on geographic and atmospheric proof-reading, decision-making tree was constructed. Plantation information about wheat, barley, and rapeseed and plantation grassland was extracted successfully. The general classification accuracy reached 86.90%. Kappa coefficient was 0.831 1. [Conclusion] Taking typical spectrum image as data source, and applying Decision Tree Method to get crops type＇s information had fine application future.
基金The National Natural Science Foundation of China(No.61375028,61301219)China Postdoctoral Science Foundation(No.2012M520973)the Scientific Research Funds of Nanjing Institute of Technology(No.ZKJ201202)
文摘In order to recognize people＇s annoyance emotions in the working environment and evaluate emotional well- being, emotional speech in a work environment is induced to obtain adequate samples of emotional speech, and a Mandarin database with two thousands samples is built. In searching for annoyance-type emotion features, the prosodic feature and the voice quality feature parameters of the emotional statements are extracted first. Then an improved back propagation （BP） neural network based on the shuffled frog leaping algorithm （SFLA） is proposed to recognize the emotion. The recognition capability of the BP, radical basis function （RBF） and the SFLA neural networks are compared experimentally. The results show that the recognition ratio of the SFLA neural network is 4. 7% better than that of the BP neural network and 4. 3% better than that of the RBF neural network. The experimental results demonstrate that the random initial data trained by the SFLA can optimize the connection weights and thresholds of the neural network, speed up the convergence and improve the recognition rate.
基金Financially supported by a grant from the Education Com-mittee of Hunan Province (No.ooAoo9)
文摘Objectives: To determine the susceptibilities of M.hominis and U. urealyticum to fluoroquinolones forthe instruction of reasonable clinical application ofantibiotics.Method: The susceptibilities of M. hominis and U.urealyticum to six fluoroquinolones were determinedby the broth dilution method.Results: Sparfloxacin and gatifloxacin were veryactive with MIC50S of 0.03125 and 0.25 μg/ml againstM. hominis, 0.25 and 0.5 μg/ml against U. urealyticum,respectively. Levofloxacin and ofloxacin had MIC50S of1 μg/ml and 2 μg/ml, respectively against both species.Norfloxacin was less effective against both species at16 and 32 μg/ml. Ciprofloxacin was unusual in thatthe MIC50S varied fourfold between species, with 2 μg/ml against M. hominis and 8 μg/ml against U.urealyticum.Conclusions: The results can provide useful infor-mation for selecting fluoroquinolones for treatmentof urogenital mycoplasma infections.
