穴位埋线关元穴、肾俞穴对慢性前列腺炎大鼠IgG和血清Zn的影响

目的:探讨简易埋线关元穴和肾俞穴对琼脂所致慢性非细菌性前列腺炎大鼠的前列腺匀浆IgG和血清Zn的影响。方法:将40只雄性SD大鼠定时定量喂养1周后,随机分为假手术组、模型对照组、肾俞组、关元组,共4组,每组10只,除假手术组外,其余各组大鼠均予以注射琼脂溶液建立模型;假手术组于同样部位、以同样方法注入氯化钠溶液。造模24 h后每7天埋线1次,连续4周。28 d后取材,采用单项免疫扩散法检测各组大鼠前列腺匀浆中IgG水平;原子吸收分光光度法检测各组大鼠前列腺血清Zn含量。结果:与假手术组相比,模型对照组的大鼠小便浑浊、活动度低、精神状态差,前列腺组织有水肿充血、粘连和硬结,前列腺匀浆中IgG含量增高,血清Zn含量降低,差异有统计学意义(P<0.05);与模型对照组对比,肾俞组和关元组的大鼠活动度、饮水量增高,小便浑浊度降低,前列腺组织仅见软性结节、粘连减少,前列腺匀浆中的IgG含量均降低,Zn含量均升高,差异有统计学意义(P<0.05)。结论:简易埋线肾俞穴和关元穴可以改善大鼠小便浑浊度和前列腺组织粘连程度,可能与降低慢性前列腺炎大鼠匀浆IgG的含量、升高血清Zn的含量有关。

前列清颗粒对大鼠慢性细菌性前列腺炎的作用

目的:研究前列清颗粒对慢性细菌性前列腺炎模型大鼠的治疗作用。方法:采用大肠埃希氏菌致慢性细菌性前列腺炎大鼠模型,设前列清颗粒高、中、低三个剂量组以及前列泰胶囊对照组、模型对照组和空白对照组,灌胃给药或蒸馏水。45天后处死大鼠,取全血、前列腺液、前列腺组织,检测全血和前列腺液中白细胞数、前列腺液中卵磷脂小体密度,并对前列腺组织进行病理学观察。结果:与模型组比较,前列清颗粒10.4、5.2、2.6g/kg均能明显降低模型大鼠的前列腺液中白细胞数及前列腺指数(P<0.05,P<0.01);前列清颗粒10.4、5.2g/kg能明显升高模型组大鼠卵磷脂小体密度(P<0.05),前列清颗粒10.4g/kg能明显降低模型组大鼠全血中白细胞数,减轻大鼠前列腺的病理改变(P<0.05)。结论:前列清颗粒对模型大鼠慢性细菌性前列腺炎有明显改善作用。

Effects of different Chinese herbal prescriptions on cytokines in autoimmune prostatitis rats

OBJECTIVE:To observe and compare the effects of Chinese herbal prescriptions for promoting blood circulation,clearing heat,removing toxicity,and dispersing stagnated liver-Qi on cytokines in model rats with experimental autoimmune prostatitis(EAP) to provide an experimental basis for the use of Chinese herbal prescriptions in the treatment of chronic prostatitis.METHODS:One-hundred and ten male Wistar rats were randomly divided into 11 groups:blank group;model group;Huoxuehuayu(promoting blood circulation to remove blood stasis) high,middle,and low dose groups;Qingrejiedu(clearing heat and removing toxicity) high,middle,and low dose groups;and Shuganliqi(dispersing stagnated liver-Qi) high,middle,and low dose groups.Except the blank group,rats in all groups were injected subcutaneously in multiple points on days 0 and 30 with prostatic protein extractive solution(60 mg/ml_),and intraperitoneally injected with diphtheria-pertussis and tetanus vaccine(DPT vaccine) to establish the EAP model.Model rats were administrated high,middle,and low doses of Chinese herbal prescriptions and were sacrificed after 4 weeks.Pathological changes in the prostate gland were observed with HE staining and changes in serum interleukin-6(IL-6),interleukin-8(IL-8),and prostaglandin E_2(PGE_2) levels were detected with enzyme-linked immunosorbent assay.RESULTS:Compared with the blank group,serum PGE_2,IL-6,and IL-8 levels in the model group were significantly higher(P < 0.05).Compared with the model group,serum PGE_2,IL-6,and IL-8 levels in the Qingrejiedu low dose and middle dose groups were significantly lower(P < 0.05),with the lower dose having a more obvious effect.Serum PGE_2,IL-6,and IL-8 levels in the Huoxuehuayu high dose group(P < 0.05),IL-6 and IL-8 levels in the Huoxuehuayu middle dose group(P < 0.05),and the IL-8level in the Huoxuehuayu low dose group were significantly lower than those in the model group(P < 0.05).There were significant differences in PGE_2 and IL-6 levels among the different dose groups of Shuganliqi drugs(P < 0.05).Compared with the model group,serum PGE_(2/) IL-6,and IL-8levels in the Shuganliqi high dose group(P <0.05) and IL-8 level in the Shuganliqi low dose group were significantly lower(P < 0.05),while the Shuganliqi middle dose group did not change significantly(P > 0.05).CONCLUSION:Therefore,in TCM treatment of autoimmune prostatitis,different treatment methods should select different doses.For prescriptions that clear heat and remove toxicity,low doses should be used.For prescriptions that promote blood circulation to remove blood stasis and for prescriptions that disperse stagnated liver-Qi,high doses should be used.