不同频度电针治疗大鼠慢性神经源性痛的疗效比较

Chronic pain, especially the chronic neuropathic pain syndrome characterized by long duration, slow recovery and difficulty to control in clinic practice makes it more serious physically and psychologically. Nowadays, the field of chro nic neuropathic pain has been grasping more and more attention. The present stud y aims at investigating the optimal interval parameter of the electroacupunctur e (EA) treatment. L 5/L 6 nerve ligation model was used to assess the effect o f EA on neuropathic pain. Mechanical allodynia was assessed by 50% paw withdrawal t hreshold and cold induced ongoing pain was detected by the number of paw lifts within 5 minutes when free rat was put on to 5±1 ℃ cold plate. Han’s Acupoint Nerve Stimulator (HANS) was connected to needles inserted into acupo ints "Jiaji"(EX B 2/L 5) and "Weizhong" (BL 40) on both sides. Other parame ters were: intensity of 0.5～ 1 mA, 1～2 mA , ten minutes each; frequency of 2 H z; and 0.6 ms in pulse width. Different interval parameter was Q 1 day (one tr eatment/day), Q 2 day, Q 3 day and Q 4 day. Results: Optimal interval pla ys a critical r ole for the analgesic effect of electroacupuncture. The interval of Q 1 day ha s a declining analgesic effect or even not any effect. The other 3 groups have accumulative effect and long lasting analgesic effect (24 hours for mechanical allodynia and 1 week for cold induced ongoing pain). Compared with the control, there is a significant difference (P<0.01 or 0.001). Among these 4 in tervals, Q 3 day group is the best. Longer lasting analgesic effect following this interval choice (48 hours for mechanical allodynia and 10 days for cold i nduced ongoing pain). Amongst the 4 interval parameters, Q 3 day group has don e better effect than the others(P<0.01 or 0.001). The results indicate that one EA treatment every 3 days is the optimal interval. And it may have som ething to do with the clinical practice.

电针对慢性神经源性痛大鼠痛行为反应和脊髓背角NOS的影响

目的:观察慢性神经源性痛时的痛行为反应和脊髓背角NOS活性变化以及电针对它们的影响作用。方法:SD大鼠72只分为对照组(n=24,假手术)、模型组(n=24,手术)、电针组(n=24,手术+电针)。慢性神经源性痛模型参照Mosconi氏的大鼠坐骨神经慢性压迫法制作。电针取“环跳”和“阳陵泉”穴区。痛阈测定采用热辐射测痛法,分别于术后第2、4、7、14、28 d进行实验观察,NOS活性观察用NADPH-d酶组织化学法。结果:慢性神经源性痛时,大鼠抬腿潜伏期(痛阈值)降低,经多次电针后,随着电针次数的增加抬腿潜伏期也逐渐升高(P<0.05)。脊髓背角的NOS活性,慢性痛大鼠明显降低,电针后又明显回升(P<0.05)。结论:慢性神经源性痛时,热痛敏的行为学表现与NOS活性的下调变化相一致,而电针对慢性神经源性痛的抑制及对脊髓背角NOS活性的表达升高有一定的作用。

电针对大鼠慢性神经源性痛的治疗作用

目的 观察大鼠慢性神经源性痛时的抬腿潜伏期变化及电针对其治疗作用。方法 72只SD大鼠制作慢性神经源性痛模型 ,随机分为对照组、慢性疼痛组、电针治疗组各 2 4只。电针取坐骨神经压迫侧环跳、阳陵泉穴。痛阈测定采用热辐射测痛法 ,术前、术后d2、d4、d7、d14、d2 8测后肢抬腿潜伏期 ,电针组并于每次电针前后各测 1次抬腿潜伏期。结果 电针治疗组术后d2开始抬腿潜伏期缩短 ,与慢性疼痛组基本相似。随电针次数增加 ,抬腿潜伏期逐渐延长 ,到d14坐骨神经压迫侧与慢性疼痛组相比明显延长 (P <0 .0 5 )。d2 8已显著延长 ,但仍低于实验对照组假手术侧 (P <0 .0 5 )。结论 多次电针后对坐骨神经压迫引起的慢性神经源性痛大鼠的热痛敏具有明显的逆转作用。

邵氏点穴位注射复方青藤碱注射液治疗慢性脊神经后支源性下腰痛临床观察

目的:观察比较电针正脊法、穴位注射法治疗慢性脊神经后支源性下腰痛的临床疗效。方法:将60例患者随机分为2组各30例,针正组采用电针加正脊治疗,穴注组在针正组基础上加用邵氏点穴位注射复方青藤碱注射液治疗。观察2组临床疗效及疼痛视觉模拟评分法(VAS)评分情况。结果:治疗后,2组VAS评分分别与治疗前比较,差异有显著性或非常显著性意义(P<0.05,P<0.01),治疗后2组VAS评分比较,差异有显著性意义(P<0.05)。2组优良率比较,差异有显著性意义(P<0.05)。结论:与电针正脊法治疗慢性脊神经后支源性下腰痛比较,加用邵氏点穴位注射复方青藤碱注射液治疗有较佳的临床疗效。