[目的]研究和解利湿方对大鼠酒精性慢性胰腺炎(ACP)及胰腺基质金属蛋白酶-2(matrix metal prote-ase-2,MMP-2)及其组织抑制因子(tissue inhibitors of metal protease-1,TIMP)-1、TIMP-2蛋白表达的影响及相关机制。[方法]Lieber-DeCarl...[目的]研究和解利湿方对大鼠酒精性慢性胰腺炎(ACP)及胰腺基质金属蛋白酶-2(matrix metal prote-ase-2,MMP-2)及其组织抑制因子(tissue inhibitors of metal protease-1,TIMP)-1、TIMP-2蛋白表达的影响及相关机制。[方法]Lieber-DeCarli酒精流质饮食联合尾静脉注射脂多糖制备大鼠ACP模型。在不同浓度(2.170g/ml、1.085g/ml、0.543g/ml)的和解利湿方治疗4周后,采用苏木精-伊红和天狼猩红染色观察胰组织病理变化,West-ern blot法检测MMP-2、TIMP-1和TIMP-2蛋白的表达。[结果]与模型对照大鼠比较,高剂量的和解利湿方减少了大鼠酒精性慢性胰腺炎炎症及纤维化病变,并增加MMP-2蛋白的表达(P<0.05),减少TIMP-1(P<0.05)和TIMP-2(P<0.01)蛋白的表达,增加MMP-2和TIMP-1的比值(P<0.01)。[结论]高剂量的和解利湿方对ACP胰腺纤维化可能有一定的治疗作用,其机制可能与抗炎、调节胰腺组织MMP-2、TIMP-1和TIMP-2蛋白的表达有关。展开更多
AIM:To assess the effectiveness of and complications associated with polyvinyl alcohol (PVA) and gelatin sponge particles embolization of splenic artery pseudoaneurysm complicating chronic alcoholic pancreatitis. METH...AIM:To assess the effectiveness of and complications associated with polyvinyl alcohol (PVA) and gelatin sponge particles embolization of splenic artery pseudoaneurysm complicating chronic alcoholic pancreatitis. METHODS: A 42-year-old man with splenic artery pseudoaneurysm formation secondary to chronic alcoholic pancreatitis was admitted. We used PVA and gelatin sponge particles embolization of splenic artery pseudoaneurysm by super-selective embolization techniques. RESULTS: The splenic artery pseudoaneurysm was successfully controlled with splenic embolization. The patient was discharged in 9 d with complete recovery. CONCLUSION: This case confirms that superselective transcatheter embolization by PVA and gelatin sponge particles may represent an effective treatment for pseudoaneurysm caused by chronic alcoholic pancreatitis in the absence of other therapeutic alternatives.展开更多
文摘[目的]研究和解利湿方对大鼠酒精性慢性胰腺炎(ACP)及胰腺基质金属蛋白酶-2(matrix metal prote-ase-2,MMP-2)及其组织抑制因子(tissue inhibitors of metal protease-1,TIMP)-1、TIMP-2蛋白表达的影响及相关机制。[方法]Lieber-DeCarli酒精流质饮食联合尾静脉注射脂多糖制备大鼠ACP模型。在不同浓度(2.170g/ml、1.085g/ml、0.543g/ml)的和解利湿方治疗4周后,采用苏木精-伊红和天狼猩红染色观察胰组织病理变化,West-ern blot法检测MMP-2、TIMP-1和TIMP-2蛋白的表达。[结果]与模型对照大鼠比较,高剂量的和解利湿方减少了大鼠酒精性慢性胰腺炎炎症及纤维化病变,并增加MMP-2蛋白的表达(P<0.05),减少TIMP-1(P<0.05)和TIMP-2(P<0.01)蛋白的表达,增加MMP-2和TIMP-1的比值(P<0.01)。[结论]高剂量的和解利湿方对ACP胰腺纤维化可能有一定的治疗作用,其机制可能与抗炎、调节胰腺组织MMP-2、TIMP-1和TIMP-2蛋白的表达有关。
文摘AIM:To assess the effectiveness of and complications associated with polyvinyl alcohol (PVA) and gelatin sponge particles embolization of splenic artery pseudoaneurysm complicating chronic alcoholic pancreatitis. METHODS: A 42-year-old man with splenic artery pseudoaneurysm formation secondary to chronic alcoholic pancreatitis was admitted. We used PVA and gelatin sponge particles embolization of splenic artery pseudoaneurysm by super-selective embolization techniques. RESULTS: The splenic artery pseudoaneurysm was successfully controlled with splenic embolization. The patient was discharged in 9 d with complete recovery. CONCLUSION: This case confirms that superselective transcatheter embolization by PVA and gelatin sponge particles may represent an effective treatment for pseudoaneurysm caused by chronic alcoholic pancreatitis in the absence of other therapeutic alternatives.
