以嵩口镇为例浅谈历史村镇“慢更新”

由于当今社会历史村镇具有保护价值但却现状没落,亟待更新与保护。以嵩口镇为例,简述驻村建设,提倡使用历史村镇"慢更新"的方法,解决历史村镇走向衰落或个性流失或失去活力等状况。

怀旧视角下老城旧街的复兴及其价值认同——以武昌昙华林街区的“慢更新”为例

城市旧街区空间的复兴可归因于怀旧心理与行为,其嵌入于快速城市化背景下的现实环境和约束条件,形构了街区空间复兴(文本)与现代城市时空结构、社会文化变迁(语境)之间的深层次交互关系。以武昌老城昙华林街区"慢更新"为例,分析了这一过程中老街区历史性与地方性的价值重构现象,认为其具有推动环境认同与文化认同的功能,有利于社会整合,同时也转变了空间更新的范式,促进了多样性空间的生成。从怀旧空间生产中的原真性与主体性角度提出了城市旧街区复兴中存在的问题及其解决路径。

Inhibition of lung cancer stem cells self-renewal and tumorigenicity by lentivirus-delivered Bmi1 shRNA

Objective： The aim of the study was to observe the effect of Bmil reduction on the self-renewal and tumorigenicity ability of lung cancer stem cells （LCSCs） in human lung adenocarcinoma. Methods： Human lung adenocarcinoma cells A549 were consecutively passaged in NOD/SCID mice treated with Paclitaxel weekly. The proportions of LCSCs in A549 cells and the cells from the third passage （A549-3rd） were compared. The expression of Bmil in LCSCs was silenced by intratumoral injection with lentivirus-delivered Broil small hairpin RNA （shRNA）. RT-PCR and Western blot were used to test the mRNA and protein expressions of Broil in LCSCs. The protein level of p16INK4A was analyzed by Western blotting. The self- renewal and tumorigenicity ability of LCSCs were evaluated by counting the sphere formation rate in serum-free medium and the tumor formation rate in NOD/SCID mice. Results： In vivo passaging ofA549 cells under chemotherapy pressure enriched for LCSCs. The expression of Broil in LCSCs increased. Down-regulation of Bmil by RNA interference resulted in reduced self-renewal and tumorigenicity ability of LCSCs and paralleled the increased expression of p16INK4A, a Bmil target. Conclu- sion： Broil regulates self-renewal and tumorigenicity of LCSCs by silencing some target genes, including p16INK4A.

历史文化街区两种更新模式的“城市融入”性探讨

本文通过对"快更新"、"慢更新"两种历史文化街区更新模式在空间、文化、功能三个层面上的城市融入性的差异对比研究,分析两者各自的优缺点,探究在两者间取得平衡的可能性,为现代城市化阶段下的历史文化街区保护发展提供建议。