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懒猴属的核糖体DNA变异及其种间分化关系 被引量:11
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作者 王文 宿兵 +5 位作者 兰宏 刘瑞清 朱春玲 佴文惠 陈玉泽 张亚平 《Zoological Research》 CAS CSCD 1996年第1期89-93,共5页
用15种限制性内切酶和人28S、18SrDNA探针构建了懒猴属各物种核糖体DNA重复单位的限制性内切酶图谱。在进化速率较高的非转录间隔区,在大、中、小懒猴中分别定位了23、24、24个酶切位点。大懒猴与中懒猴有13个... 用15种限制性内切酶和人28S、18SrDNA探针构建了懒猴属各物种核糖体DNA重复单位的限制性内切酶图谱。在进化速率较高的非转录间隔区,在大、中、小懒猴中分别定位了23、24、24个酶切位点。大懒猴与中懒猴有13个位点不同,与小懒猴有14个位点不同,而中、小懒猴间则只有一个位点的差异。经过计算,大懒猴与中懒猴的遗传距离值为12.65%,与小懒猴的差异为14.24%,中、小懒猴间的差异则仅为0.71%。结合形态学、细胞学、线粒体DNA和前人在其它动物类群中rDNA变异的数据表明,懒猴属内似只有两个有效物种,即大懒猴和小懒猴,中懒猴与小懒猴的分化至多到半种级别。 展开更多
关键词 灵长目 懒猴属 核糖体DNA 种间分化
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Molecular Phylogeny of Slow Lorises (Nycticebus) Revealed by D-loop Sequences and Complete Cytochrome b Gene Sequences of Mitochondrial DNA
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作者 陈静华 Paul CRO W +2 位作者 成岛悦雄 张红卫 张亚平 《Zoological Research》 CAS CSCD 北大核心 2004年第4期292-297,共6页
Partial sequences of the D-loop and the complete sequences of cytochrome b gene (1 140 bp) of the slow lorises (genus Nycticebus) were undertaken to investigate evolutionary relationships among species of Nycticebus.S... Partial sequences of the D-loop and the complete sequences of cytochrome b gene (1 140 bp) of the slow lorises (genus Nycticebus) were undertaken to investigate evolutionary relationships among species of Nycticebus.Sequence analysis results consistently provide new taxonomy evidence at the DNA level for supporting Ratajszczak and Groves’ viewpoint that N.intermedus is merely the adult of N.pygmaeus (Ratajszczak,1998;Groves,1971).Phylogenetic analysis was performed by means of the combined data and these two separate sequences data,respectively,by using various methods,supporting the same topology,in which genus Nycticebus was formed of two clusters.The first cluster was composed of N.pygmaeus,and the second cluster of N.coucang.It also could provide a new molecular genetic evidence to support the view that the genus comprises two species:N.coucang and N.pygmaeus. 展开更多
关键词 Nycticebus Mitochondiral DNA Cytochrome b gene D-LOOP Molecular phylogeny
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