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胎儿皮肤与成人皮肤的形态结构及表皮干细胞分子标志物的比较 被引量:1
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作者 吴永胜 陈晓蓉 《安徽医科大学学报》 CAS 北大核心 2016年第12期1781-1784,共4页
目的探讨胎儿皮肤与成人皮肤的形态结构及表皮干细胞分子标志物的差异,为后续皮肤功能的研究及皮肤移植提供理论依据。方法取胎儿背部与成人腿部皮肤,标本取材后立即用10%中性甲醛固定,石蜡包埋。所有标本经石蜡切片,HE染色,观察皮肤表... 目的探讨胎儿皮肤与成人皮肤的形态结构及表皮干细胞分子标志物的差异,为后续皮肤功能的研究及皮肤移植提供理论依据。方法取胎儿背部与成人腿部皮肤,标本取材后立即用10%中性甲醛固定,石蜡包埋。所有标本经石蜡切片,HE染色,观察皮肤表皮、真皮和皮肤附属器的形态结构;应用免疫组织化学法检测皮肤组织中表皮干细胞标志物p63、β1整合素和角蛋白19(K19)的表达。结果胎儿皮肤与成人皮肤表皮、真皮和皮肤附属器结构不同,胎儿表皮由2~3层上皮细胞组成,基底层细胞呈立方形,核圆,染色深,未发现颗粒层细胞,真皮乳头状突起不明显;成人全层皮肤明显厚于胎儿皮肤,尤其是成人皮肤的真皮层明显较胎儿真皮发达,成人皮肤真皮乳头状突起明显,成人表皮由6~7层上皮细胞组成,基底层细胞呈立方形或柱形,细胞核染色较深,呈卵圆形,居中,有明显的颗粒层,但细胞轮廓不清晰,胞质内可见蓝色颗粒。经测定胎儿皮肤表达的p63、β1整合素及K19的平均光密度值高于成人(P〈0.01)。结论胎儿皮肤和成人皮肤表皮、真皮和皮肤附属器形态结构存在差异,表皮干细胞分子标志物表达也存在差异。 展开更多
关键词 胎儿皮肤 成人皮肤 表皮干细胞 形态结构 免疫组织化学
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盐酸丁卡因喷雾剂及护理干预在成人急性皮肤挫擦伤清创术中的应用
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作者 黄国梅 刘丽瑜 车素云 《实用临床医学(江西)》 CAS 2016年第11期92-93,95,共3页
目的探讨盐酸丁卡因喷雾剂及护理干预在成人急性皮肤挫擦伤清创术中的应用效果。方法抽取门急诊132例行清创术治疗的急性皮肤挫擦伤成人患者,根据就诊先后顺序分为研究组与对照组,各66例。对照组采用生理盐水及常规护理干预,研究组采用... 目的探讨盐酸丁卡因喷雾剂及护理干预在成人急性皮肤挫擦伤清创术中的应用效果。方法抽取门急诊132例行清创术治疗的急性皮肤挫擦伤成人患者,根据就诊先后顺序分为研究组与对照组,各66例。对照组采用生理盐水及常规护理干预,研究组采用盐酸丁卡因喷雾剂及心理、饮食护理。对比治疗前后疼痛评分变化情况及护理满意度。结果护理治疗后,研究组疼痛评分明显少于对照组[(3.1±1.1)分比(5.9±2.3)分,P<0.05];研究组护理总满意度高于对照组(96.97%比83.33%,P<0.05)。研究组创面止血时间、创面继发感染率、愈合时间均明显低于对照组(P<0.05)。结论应用盐酸丁卡因喷雾剂治疗成人急性皮肤挫擦伤效果确切,且实施心理及饮食干预可有效缓解患者疼痛感,提高护理满意度。 展开更多
关键词 盐酸丁卡因喷雾剂 成人急性皮肤挫擦伤 镇痛 护理
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聚左旋乳酸-聚乙二醇聚合物对成人皮肤成纤维细胞增殖影响
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作者 周业松 余奕文 +2 位作者 陈丽媛 央金 毛钰婷 《中文科技期刊数据库(全文版)医药卫生》 2019年第3期69-71,共3页
体外培养正常成人皮肤成纤维细胞(原代),并对成纤维细胞进行鉴定,观察聚左旋乳酸-聚乙二醇聚合物(PLLA-PEG)对正常成人皮肤成纤维细胞增殖以及I型胶原蛋白合成的影响,探讨其对成人皮肤成纤维细胞增殖的潜在作用。方法:体外培养成人正常... 体外培养正常成人皮肤成纤维细胞(原代),并对成纤维细胞进行鉴定,观察聚左旋乳酸-聚乙二醇聚合物(PLLA-PEG)对正常成人皮肤成纤维细胞增殖以及I型胶原蛋白合成的影响,探讨其对成人皮肤成纤维细胞增殖的潜在作用。方法:体外培养成人正常皮肤成纤维细胞,利用MTT和ELISA分别检测不同浓度PLLA-PEG对成人皮肤成纤维细胞活性、I型胶原蛋白合成影响。结果:经PLLA-PEG作用后细胞数量明显增加,并且在一定范围内随PLLA-PEG的增加而增多,同时促进细胞分泌及合成I型胶原蛋白。结论:PLLA-PEG能刺激人皮肤成纤维细胞的生长及增殖,刺激成纤维细胞分泌I型胶原蛋白,是一种有效的除皱、紧肤、补水的产品。 展开更多
关键词 聚左旋乳酸-聚乙二醇 PLLA-PEG 成人皮肤成纤维细胞 I型胶原蛋白 增殖及合成
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体外扩增源于成人头皮组织的真皮细胞具有多向分化潜能
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作者 周倩 张群 +4 位作者 冷雪 俎廷建 秦静 温洁 吴训伟 《智慧健康》 2017年第3期1-5,共5页
目的探讨来源于成人头皮组织的真皮细胞经体外培养扩增后其向成脂、神经、成骨及成肌方向分化的潜能,为皮肤创伤修复种子细胞的新来源提供依据。方法取胚胎及成人头皮组织分离真皮细胞。利用维持细胞多功能性的培养基传代培养,取第4代... 目的探讨来源于成人头皮组织的真皮细胞经体外培养扩增后其向成脂、神经、成骨及成肌方向分化的潜能,为皮肤创伤修复种子细胞的新来源提供依据。方法取胚胎及成人头皮组织分离真皮细胞。利用维持细胞多功能性的培养基传代培养,取第4代真皮细胞免疫荧光染色,通过检测波形蛋白和角蛋白的表达进行细胞鉴定。对真皮细胞进行定向分化诱导并用免疫荧光染色检测各方向分化指标蛋白,通过和源于胚胎头皮组织的真皮细胞比较,来分析其多向分化潜能。结果原代细胞分离后24h贴壁,细胞形态多为梭形或多角形,可多次传代且传代后细胞形态均一稳定。免疫荧光染色结果显示该细胞仅表达波形蛋白(vimentin)不表达角质蛋白(Pan-CK);源于成人及胚胎头皮的真皮细胞均能诱导向成脂、成骨、成神经及成肌细胞方向分化,尽管与源于胚胎组织的细胞相比,成人真皮细胞分化效率相对较低。结论源于头皮分离的成人真皮细胞经培养扩增后能诱导分化成不同组织细胞,因而具有多向分化潜能。由于成人头皮组织相对容易获得,其来源广泛,因而可成为皮肤组织工程研究以及临床应用的理想种子细胞。 展开更多
关键词 成人皮肤组织 真皮细胞 波形蛋白 角蛋白 多向分化
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白癜风患者生活质量调查与分析 被引量:2
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作者 徐智广 王芳玲 王欣 《河南中医》 2012年第10期1309-1311,共3页
目的:研究影响白癜风患者生活质量的因素,提高人们对白癜风患者生活质量的关注。方法:采用世界卫生组织生活质量测定量表简表(WHOQOL-BREF)中文版和成人皮肤病生活质量指标问卷(DLQI),对河北省沧州中西医结合医院皮肤科就诊,符合纳入标... 目的:研究影响白癜风患者生活质量的因素,提高人们对白癜风患者生活质量的关注。方法:采用世界卫生组织生活质量测定量表简表(WHOQOL-BREF)中文版和成人皮肤病生活质量指标问卷(DLQI),对河北省沧州中西医结合医院皮肤科就诊,符合纳入标准的160位患者进行生活质量测查。结果:①WHOQOL-BREF得分,生理领域、心理领域低于常模;社会关系领域、环境领域高于常模。②DLQI评分,平均(5.76±4.58)分。白癜风患者DLQI得分主要分布在2~5,6~11两个阶段,有13.13%的患者得分达12分以上。结论:①白癜风患者的生理和心理状态较差,大部分患者对健康状况主观感觉不满意。心理方面,主要是对疾病和相貌的担心。白癜风患者的社会支持并未因为患病而减退。②暴露部位皮疹较严重影响患者的生活质量。女性患者在生理感受方面较男性更敏感,更注重形象,白癜风严重影响了女性患者着装、穿戴的选择。暴露部位的白癜风患者尴尬或沮丧感觉明显,严重影响社交、外出或娱乐活动,进而,影响患者生活质量。白癜风对21~30岁患者朋友关系影响较大。白癜风对无家族史患者在社交、外出或娱乐活动方面影响更大。 展开更多
关键词 白癜风 生活质量测定量表简表中文版 成人皮肤病生活质量指标问卷
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Mechanistic Insights of Cells in Porous Scaffolds via Integrated Culture Technologies
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作者 Christopher Michael Gabbott ] Tao Sun 《Journal of Life Sciences》 2017年第4期163-175,共13页
This research aimed to combine 3 cell and tissue culture technologies to obtain mechanistic insights of cells in porous scaffolds. When cultivated on 2D (2-dimensional) surfaces, HDFs (human dermal fibroblasts) be... This research aimed to combine 3 cell and tissue culture technologies to obtain mechanistic insights of cells in porous scaffolds. When cultivated on 2D (2-dimensional) surfaces, HDFs (human dermal fibroblasts) behaved individually and had no strict requirement on seeding density for proliferation; while HaCat cells relied heavily on initial densities for proliferation and colony formation, which was facilitated when co-cultured with HDFs. Experiments using a 3D CCIS (3-dimensional cell culture and imaging system) indicated that HDFs colonised openpores of varying sizes (125-420 ~tm) on modular substrates via bridge structures; while HaCat cells formed aperture structures and only colonised small pores (125 txm). When co-cultured, HDFs not only facilitated HaCat attachment on the substrates, but also coordinated with HaCat cells to colonise open pores of varying sizes via bridge and aperture structures. Based on these observations, a 2-stage strategy for the culture of HDFs and HaCat cells on porous scaffolds was proposed and applied successfully on a cellulosic scaffold. This research demonstrated that cell colonisation in scaffolds was dependent on multiple factors; while the integrated 2D&3D culture technologies and the 3D CCIS was an effective and efficient approach to obtain mechanistic insights of their influences on tissue regeneration. 展开更多
关键词 Porous scaffold cell colonisation mechanistic understanding 2D cell culture 3D tissue culture scale-down design.
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Treatment of Cough and Dyspnea due to Acute Bronchitis by Plaster for Cough and Dyspnea——A Report of 735 Cases
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作者 陈振甫 周文秀 +2 位作者 高举先 孙江桥 周永生 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2002年第1期5-8,共4页
In the light of the theory of treating the internal disease externally, an externally used plaster for treating cough and dyspnea due to acute bronchitis (Ke Chuan Yi Tie Kang [symbol: see text]-[symbol: see text]) wa... In the light of the theory of treating the internal disease externally, an externally used plaster for treating cough and dyspnea due to acute bronchitis (Ke Chuan Yi Tie Kang [symbol: see text]-[symbol: see text]) was successfully applied to 735 cases of acute bronchitis (the treatment group), with the other 423 cases treated with routine western drugs as controls. The results showed that the cure rate in the treatment group was significantly higher than that in the control group (P 展开更多
关键词 Acupuncture Points Acute Disease Administration Cutaneous Adolescent Adult BRONCHITIS CHILD Child Preschool Comparative Study COUGH Drugs Chinese Herbal DYSPNEA Female Humans Infant Male Middle Aged
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Intramedullary nailing of clavicular midshaft fractures in adults using titanium elastic nail 被引量:6
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作者 CHEN Qing-yu KOU Dong-quan CHENG Xiao-jie ZHANG Wei WANG Wei LIN Zhang-qin CHENG Shao-wen SHEN Yue YING Xiao-zhou PENG Lei LU Chuan-zhu 《Chinese Journal of Traumatology》 CAS 2011年第5期269-276,共8页
Objective: Studies showed elastic stable intramedullary nailing (ESIN) of displaced midclavicular fractures has excellent outcomes, as well as high complication rates and specific problems. The aim was to discuss E... Objective: Studies showed elastic stable intramedullary nailing (ESIN) of displaced midclavicular fractures has excellent outcomes, as well as high complication rates and specific problems. The aim was to discuss ESIN of midshaft clavicular fractures.Methods: Totally 60 eligible patients (aged 18-63 years) were randomized to either ESIN group or non-operative group between January 2007 and May 2008. Clavicular shortening was measured after trauma and osseous consolidation.Radiographic union and complications were assessed. Function analysis including Constant shoulder scores and disabilities of the arm, shoulder and hand (DASH) scores were performed after a 15-month follow-up.Results: ESIN led to a signifcantly shorter time to union, especially for simple fractures. In ESIN group, all patients got fracture union, of which 5 cases had medial skin irritation and 1 patient needed revision surgery because of implant failure. In the nonoperative group, there were 3 nonunion cases and 2 symptomatic malunions developed requiring corrective osteotomy. At 15 months after intramedullary stabilization, patients in the ESIN group were more satisfied with the appearance of the shoulder and overall outcome, and they benefited a lot from the great improvement of post-traumatic clavicular shortening. Furthermore,DASH scores were lower and Constant scores were significantly higher in contrast to the non-operative group.Conclusion: ESIN is a safe minimally invasive surgical technique with lower complication rate, faster return to daily activities, excellent cosmetic and better functional results,restoration of clavicular length for treating mid-shaft clavicular fractures, resulting in high overall satisfaction, which can be regard as an alternative to plate fixation or nonoperative treatment of mid-shaft clavicular fractures. 展开更多
关键词 CLAVICLE Fracture fixation intramedullary Outcome assessment
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Construction of the subtractive cDNA library of injured adult and fetal rabbit skins 被引量:1
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作者 张波 刘大维 +3 位作者 王正国 朱佩芳 周继红 蒋建新 《Chinese Journal of Traumatology》 CAS 2004年第1期13-17,共5页
Objective: Early gestational mammalian fetuses possess the amazing ability to heal cutaneous wounds in a scarless fashion. Over the past years, scientists have been working to decipher the mechanisms underlying this r... Objective: Early gestational mammalian fetuses possess the amazing ability to heal cutaneous wounds in a scarless fashion. Over the past years, scientists have been working to decipher the mechanisms underlying this regenerative repair. The remarkable phenotypic differences between fetal and adult healings behoves us to learn their characteristics in genetics, which represents potentially important mechanisms involved in wound repair observed in fetal versus adult tissues. In this sense, it is reasonable to construct subtractive cDNA library for future research. Methods: Middle laparotomy and hysterotomy were performed on pregnant rabbits at 20 day gestation to expose the fetal back, and a longitudinal incision through the skin was made on the back of the fetus. The traumatized fetal skin was harvested 12 hours post operation, the fetus control and traumatized adult skin specimens were taken at the same time. dscDNA was synthesized from total RNA of skin samples with SMART technology. Taking one of the three samples as Tester respectively and the other two as Drivers, we obtained 1 forward and 2 reverse hybridization products. After being amplified with selective polymerase chain reaction, the products were inserted into a vector, and then transferred into E.coli HB101. The colonies were screened afterwards. Results: The wounded fetuses were alive for a long time even after birth. Every determinant step, such as RNA isolation, cDNA synthesis, Rsa I digestion, adaptor ligation and hybridization, was well operated. Subtractive efficiency identification demonstrated that the suppression subtractive hybridization (SSH) was successful. Insertion into vector and transferring to E.coli were satisfactory. Conclusions: Instead of classic SSH, an improved SSH with 2 Drivers was applied for the experiment. Results confirmed that the improved program was reasonable and correct in both theory and practice. The subtractive cDNA library we have obtained is going to be used for future researches to reveal scarless healing related gene(s) and its (their) expression. 展开更多
关键词 Wound healing CICATRIX Gene library Suppression subtractive hybridization genes
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