旋流火焰燃烧温度场二维吸收光谱诊断

针对旋流火焰的复杂流场结构,结合可调谐二极管吸收光谱技术(tunable diode laser absorption spectroscopy , TDLAS)和多光谱层析成像术(hyperspectral tomography , HT),发展了具有空间分辨能力的二维吸收光谱测量技术(tunable diode laser absorption tomography , TDLAT),实现了甲烷/空气旋流火焰不同高度的二维温度场测量.该TDLAT系统吸收波长为7 185.6 , 7 444.3 , 6 807.8和7 466.3 cm -1 四线,采用分时-直接吸收探测策略,测量频率2.5 kHz ,采用13×13路正交光路(空间分辨率7 mm),采用模拟退火算法进行数据重建.经与理论计算结果对比分析,重建结果真实地反映了旋流火焰温度场的二维分布.

成像光谱技术中机上实时数据压缩方法研究

根据成像光谱仪的特点用一系列串行的一维压缩实现二维图像压缩.提出了在机上对原始数据从光谱方向进行实时压缩的“二真值线性预测方法”.该法可做到压缩/复原后光谱特征信息不丢失.实验表明,对大多数光谱数据在1%的重建精度下,至少可获得2:1的压缩比.从总体而言,压缩后原始数据率可降低3～4倍.文中还对压缩数据的编码和误差进行了讨论.

扫描多光谱显微成像

文本描述了一种新的显微成像方法,它把光学扫描显微成像技术与成像多光谱技术巧妙地结合在一起,形成扫描多光谱显微成像技术.文中叙述了系统的结构及工作原理,介绍了所采用的图像处理系统,给出了实验结果,并进行了分析讨论.

Applications of multiplexed immunohistochemistry/immunofluorescence and multispectral imaging technology in the field of tumor immunotherapy

Multiplexed immunohistochemistry/fluorescence(mIHC/IF)in combination with multispectral unmixing is a novel multitarget histopathological staining and imaging technique.By simultaneously revealing expression level and spatial information for up to eight biomarkers in situ,in addition to a nuclear stain within a single formalin-fixed paraffin-embedded(FFPE)tissue section,this technology can analyze the phenotype,abundance,morphology and intercellular relationship of cells while providing statistically significant results.In recent years,technical improvements have brought new insight into mIHC/IF and multispectral imaging approaches,which have been successfully applied in the field of cancer immunotherapy,specifically in regard to tumor microenvironment research,immunotherapy drug discovery,and prognostic and metastatic risk evaluation.This review highlights the principle,workflow,advantages and disadvantages of the technology,and discusses the latest applications of mIHC/IF-based imaging technology in the field of TME-related research and immunotherapy drug discovery.